Monitoring Translocations by M-FISH and Three-color FISH Painting Techniques：A Study of Two Radiotherapy Patients
Purpose：To compare translocation rate using either M-FISH or FISH-3 in two patients treated for head and neck cancer, with a view to retrospective dosimetry. Materials and methods：Translocation analysis was performed on peripheral blood lymphocyte cultures from blood samples taken at different times...
Gespeichert in:
| Veröffentlicht in: | JOURNAL OF RADIATION RESEARCH 2007, Vol.48 (5), p.425-434 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 434 |
|---|---|
| container_issue | 5 |
| container_start_page | 425 |
| container_title | JOURNAL OF RADIATION RESEARCH |
| container_volume | 48 |
| creator | Pouzoulet, F Roch-Lefèvre, S Giraudet, A L Vaurijoux, A Voisin, Pa Buard, V Delbos, M Bourhis, J Voisin, Ph Roy, Laurence |
| description | Purpose：To compare translocation rate using either M-FISH or FISH-3 in two patients treated for head and neck cancer, with a view to retrospective dosimetry. Materials and methods：Translocation analysis was performed on peripheral blood lymphocyte cultures from blood samples taken at different times during the radiotherapy (0 Gy, 12 Gy and 50 Gy) and a few months after the end of the treatment (follow-up). Results：Estimated translocation yield varied according to the FISH technique used. At 50 Gy and follow-up points, the translocation yields were higher with FISH-3 than with M-FISH. This difference can be attributed to three events. First, an increase in complex aberrations was observed for 50 Gy and follow-up points compared with 0 Gy and 12 Gy points. Second, at the end of treatment for patient A, involvement of chromosomes 2, 4, 12 in translocations was less than expected according to the Lucas formula. Third, a clone bearing a translocation involving a FISH-3 painted chromosome was detected. Conclusions：More translocations were detected with M-FISH than with FISH-3, and so M-FISH is expected to improve the accuracy of chromosome aberration analyses in some situations. |
| doi_str_mv | 10.1269/jrr.07013 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_proquest_miscellaneous_68315829</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68315829</sourcerecordid><originalsourceid>FETCH-LOGICAL-c631t-32e43d5d6ad59189a8af6607b5cc8ae59936e0b8b5afa2b9abe5534ff5c144103</originalsourceid><addsrcrecordid>eNpFkU2OEzEQhS0EYqJhFlwAeYXEoody-6ftZTQiZKSMQExYW263mzh07GB3g3IVzsKduALOj5hVPVV99aSqh9BrArekFur9NqVbaIDQZ2hGKFOVIrx5jmbAiqYg4Ard5OxbIFwASEJeoivSNJIr2sxQfojBjzH58A2vkwl5iNaMPoaM2wN-qBb3j0tsQofXm-RcZeMQEz41PxsfxtOas5vgf0wu__3ze44fx6k74Njj9a-Iv5jOx3HjktkfysboXRjzK_SiN0N2N5d6jb4uPqzvltXq08f7u_mqsoKSsaK1Y7TjnTAdV0QqI00vBDQtt1Yax5WiwkErW256U7fKtI5zyvqeW8IYAXqN3p19N2bQ--R3Jh10NF4v5yt97AEFyamsf5LCvj2z-xSPp4x657N1w2CCi1PWQlLCZa2eTG2KOSfX_3cmoI-B6BKIPgVS2DcX06ndue6JvLy_AIszUKbemiGGwQent3FKoXxG2-9iG1NyugZoNACTwEtRGlhdBKNMSs6hof8A9YqdLQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68315829</pqid></control><display><type>article</type><title>Monitoring Translocations by M-FISH and Three-color FISH Painting Techniques：A Study of Two Radiotherapy Patients</title><source>J-STAGE Free</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Access via Oxford University Press (Open Access Collection)</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Open Access Titles of Japan</source><source>Free Full-Text Journals in Chemistry</source><creator>Pouzoulet, F ; Roch-Lefèvre, S ; Giraudet, A L ; Vaurijoux, A ; Voisin, Pa ; Buard, V ; Delbos, M ; Bourhis, J ; Voisin, Ph ; Roy, Laurence</creator><creatorcontrib>Pouzoulet, F ; Roch-Lefèvre, S ; Giraudet, A L ; Vaurijoux, A ; Voisin, Pa ; Buard, V ; Delbos, M ; Bourhis, J ; Voisin, Ph ; Roy, Laurence ; Rue Camille Desmoulins ; Institut de Radioprotection et de Surete Nucleaire ; PR ; ; Laboratoire UPRES EA ; Laboratoire de Dosimetrie Biologique ; "Radiosensibilite des tumeurs et tissus sains"</creatorcontrib><description>Purpose：To compare translocation rate using either M-FISH or FISH-3 in two patients treated for head and neck cancer, with a view to retrospective dosimetry. Materials and methods：Translocation analysis was performed on peripheral blood lymphocyte cultures from blood samples taken at different times during the radiotherapy (0 Gy, 12 Gy and 50 Gy) and a few months after the end of the treatment (follow-up). Results：Estimated translocation yield varied according to the FISH technique used. At 50 Gy and follow-up points, the translocation yields were higher with FISH-3 than with M-FISH. This difference can be attributed to three events. First, an increase in complex aberrations was observed for 50 Gy and follow-up points compared with 0 Gy and 12 Gy points. Second, at the end of treatment for patient A, involvement of chromosomes 2, 4, 12 in translocations was less than expected according to the Lucas formula. Third, a clone bearing a translocation involving a FISH-3 painted chromosome was detected. Conclusions：More translocations were detected with M-FISH than with FISH-3, and so M-FISH is expected to improve the accuracy of chromosome aberration analyses in some situations.</description><identifier>ISSN: 0449-3060</identifier><identifier>EISSN: 1349-9157</identifier><identifier>DOI: 10.1269/jrr.07013</identifier><identifier>PMID: 17785937</identifier><language>eng</language><publisher>England: THE JAPAN RADIATION RESEARCH SOCIETY</publisher><subject>Aged ; Chromosome Painting - methods ; Head and Neck Neoplasms - genetics ; Head and Neck Neoplasms - radiotherapy ; Humans ; In Situ Hybridization, Fluorescence - methods ; Life Sciences ; Male ; Microscopy, Fluorescence, Multiphoton - methods ; Middle Aged ; Reproducibility of Results ; Sensitivity and Specificity ; Translocation, Genetic - genetics ; Translocation, Genetic - radiation effects</subject><ispartof>JOURNAL OF RADIATION RESEARCH, 2007, Vol.48 (5), p.425-434</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c631t-32e43d5d6ad59189a8af6607b5cc8ae59936e0b8b5afa2b9abe5534ff5c144103</citedby><cites>FETCH-LOGICAL-c631t-32e43d5d6ad59189a8af6607b5cc8ae59936e0b8b5afa2b9abe5534ff5c144103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,866,887,4028,27932,27933,27934</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17785937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03085382$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Pouzoulet, F</creatorcontrib><creatorcontrib>Roch-Lefèvre, S</creatorcontrib><creatorcontrib>Giraudet, A L</creatorcontrib><creatorcontrib>Vaurijoux, A</creatorcontrib><creatorcontrib>Voisin, Pa</creatorcontrib><creatorcontrib>Buard, V</creatorcontrib><creatorcontrib>Delbos, M</creatorcontrib><creatorcontrib>Bourhis, J</creatorcontrib><creatorcontrib>Voisin, Ph</creatorcontrib><creatorcontrib>Roy, Laurence</creatorcontrib><creatorcontrib>Rue Camille Desmoulins</creatorcontrib><creatorcontrib>Institut de Radioprotection et de Surete Nucleaire</creatorcontrib><creatorcontrib>PR</creatorcontrib><creatorcontrib>Laboratoire UPRES EA</creatorcontrib><creatorcontrib>Laboratoire de Dosimetrie Biologique</creatorcontrib><creatorcontrib>"Radiosensibilite des tumeurs et tissus sains"</creatorcontrib><title>Monitoring Translocations by M-FISH and Three-color FISH Painting Techniques：A Study of Two Radiotherapy Patients</title><title>JOURNAL OF RADIATION RESEARCH</title><addtitle>J Radiat Res</addtitle><description>Purpose：To compare translocation rate using either M-FISH or FISH-3 in two patients treated for head and neck cancer, with a view to retrospective dosimetry. Materials and methods：Translocation analysis was performed on peripheral blood lymphocyte cultures from blood samples taken at different times during the radiotherapy (0 Gy, 12 Gy and 50 Gy) and a few months after the end of the treatment (follow-up). Results：Estimated translocation yield varied according to the FISH technique used. At 50 Gy and follow-up points, the translocation yields were higher with FISH-3 than with M-FISH. This difference can be attributed to three events. First, an increase in complex aberrations was observed for 50 Gy and follow-up points compared with 0 Gy and 12 Gy points. Second, at the end of treatment for patient A, involvement of chromosomes 2, 4, 12 in translocations was less than expected according to the Lucas formula. Third, a clone bearing a translocation involving a FISH-3 painted chromosome was detected. Conclusions：More translocations were detected with M-FISH than with FISH-3, and so M-FISH is expected to improve the accuracy of chromosome aberration analyses in some situations.</description><subject>Aged</subject><subject>Chromosome Painting - methods</subject><subject>Head and Neck Neoplasms - genetics</subject><subject>Head and Neck Neoplasms - radiotherapy</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Microscopy, Fluorescence, Multiphoton - methods</subject><subject>Middle Aged</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Translocation, Genetic - genetics</subject><subject>Translocation, Genetic - radiation effects</subject><issn>0449-3060</issn><issn>1349-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU2OEzEQhS0EYqJhFlwAeYXEoody-6ftZTQiZKSMQExYW263mzh07GB3g3IVzsKduALOj5hVPVV99aSqh9BrArekFur9NqVbaIDQZ2hGKFOVIrx5jmbAiqYg4Ard5OxbIFwASEJeoivSNJIr2sxQfojBjzH58A2vkwl5iNaMPoaM2wN-qBb3j0tsQofXm-RcZeMQEz41PxsfxtOas5vgf0wu__3ze44fx6k74Njj9a-Iv5jOx3HjktkfysboXRjzK_SiN0N2N5d6jb4uPqzvltXq08f7u_mqsoKSsaK1Y7TjnTAdV0QqI00vBDQtt1Yax5WiwkErW256U7fKtI5zyvqeW8IYAXqN3p19N2bQ--R3Jh10NF4v5yt97AEFyamsf5LCvj2z-xSPp4x657N1w2CCi1PWQlLCZa2eTG2KOSfX_3cmoI-B6BKIPgVS2DcX06ndue6JvLy_AIszUKbemiGGwQent3FKoXxG2-9iG1NyugZoNACTwEtRGlhdBKNMSs6hof8A9YqdLQ</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Pouzoulet, F</creator><creator>Roch-Lefèvre, S</creator><creator>Giraudet, A L</creator><creator>Vaurijoux, A</creator><creator>Voisin, Pa</creator><creator>Buard, V</creator><creator>Delbos, M</creator><creator>Bourhis, J</creator><creator>Voisin, Ph</creator><creator>Roy, Laurence</creator><general>THE JAPAN RADIATION RESEARCH SOCIETY</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>2007</creationdate><title>Monitoring Translocations by M-FISH and Three-color FISH Painting Techniques：A Study of Two Radiotherapy Patients</title><author>Pouzoulet, F ; Roch-Lefèvre, S ; Giraudet, A L ; Vaurijoux, A ; Voisin, Pa ; Buard, V ; Delbos, M ; Bourhis, J ; Voisin, Ph ; Roy, Laurence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c631t-32e43d5d6ad59189a8af6607b5cc8ae59936e0b8b5afa2b9abe5534ff5c144103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aged</topic><topic>Chromosome Painting - methods</topic><topic>Head and Neck Neoplasms - genetics</topic><topic>Head and Neck Neoplasms - radiotherapy</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Microscopy, Fluorescence, Multiphoton - methods</topic><topic>Middle Aged</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Translocation, Genetic - genetics</topic><topic>Translocation, Genetic - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pouzoulet, F</creatorcontrib><creatorcontrib>Roch-Lefèvre, S</creatorcontrib><creatorcontrib>Giraudet, A L</creatorcontrib><creatorcontrib>Vaurijoux, A</creatorcontrib><creatorcontrib>Voisin, Pa</creatorcontrib><creatorcontrib>Buard, V</creatorcontrib><creatorcontrib>Delbos, M</creatorcontrib><creatorcontrib>Bourhis, J</creatorcontrib><creatorcontrib>Voisin, Ph</creatorcontrib><creatorcontrib>Roy, Laurence</creatorcontrib><creatorcontrib>Rue Camille Desmoulins</creatorcontrib><creatorcontrib>Institut de Radioprotection et de Surete Nucleaire</creatorcontrib><creatorcontrib>PR</creatorcontrib><creatorcontrib>Laboratoire UPRES EA</creatorcontrib><creatorcontrib>Laboratoire de Dosimetrie Biologique</creatorcontrib><creatorcontrib>"Radiosensibilite des tumeurs et tissus sains"</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>JOURNAL OF RADIATION RESEARCH</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pouzoulet, F</au><au>Roch-Lefèvre, S</au><au>Giraudet, A L</au><au>Vaurijoux, A</au><au>Voisin, Pa</au><au>Buard, V</au><au>Delbos, M</au><au>Bourhis, J</au><au>Voisin, Ph</au><au>Roy, Laurence</au><aucorp>Rue Camille Desmoulins</aucorp><aucorp>Institut de Radioprotection et de Surete Nucleaire</aucorp><aucorp>PR</aucorp><aucorp>Laboratoire UPRES EA</aucorp><aucorp>Laboratoire de Dosimetrie Biologique</aucorp><aucorp>"Radiosensibilite des tumeurs et tissus sains"</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring Translocations by M-FISH and Three-color FISH Painting Techniques：A Study of Two Radiotherapy Patients</atitle><jtitle>JOURNAL OF RADIATION RESEARCH</jtitle><addtitle>J Radiat Res</addtitle><date>2007</date><risdate>2007</risdate><volume>48</volume><issue>5</issue><spage>425</spage><epage>434</epage><pages>425-434</pages><issn>0449-3060</issn><eissn>1349-9157</eissn><abstract>Purpose：To compare translocation rate using either M-FISH or FISH-3 in two patients treated for head and neck cancer, with a view to retrospective dosimetry. Materials and methods：Translocation analysis was performed on peripheral blood lymphocyte cultures from blood samples taken at different times during the radiotherapy (0 Gy, 12 Gy and 50 Gy) and a few months after the end of the treatment (follow-up). Results：Estimated translocation yield varied according to the FISH technique used. At 50 Gy and follow-up points, the translocation yields were higher with FISH-3 than with M-FISH. This difference can be attributed to three events. First, an increase in complex aberrations was observed for 50 Gy and follow-up points compared with 0 Gy and 12 Gy points. Second, at the end of treatment for patient A, involvement of chromosomes 2, 4, 12 in translocations was less than expected according to the Lucas formula. Third, a clone bearing a translocation involving a FISH-3 painted chromosome was detected. Conclusions：More translocations were detected with M-FISH than with FISH-3, and so M-FISH is expected to improve the accuracy of chromosome aberration analyses in some situations.</abstract><cop>England</cop><pub>THE JAPAN RADIATION RESEARCH SOCIETY</pub><pmid>17785937</pmid><doi>10.1269/jrr.07013</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0449-3060 |
| ispartof | JOURNAL OF RADIATION RESEARCH, 2007, Vol.48 (5), p.425-434 |
| issn | 0449-3060 1349-9157 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68315829 |
| source | J-STAGE Free; MEDLINE; DOAJ Directory of Open Access Journals; Access via Oxford University Press (Open Access Collection); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | Aged Chromosome Painting - methods Head and Neck Neoplasms - genetics Head and Neck Neoplasms - radiotherapy Humans In Situ Hybridization, Fluorescence - methods Life Sciences Male Microscopy, Fluorescence, Multiphoton - methods Middle Aged Reproducibility of Results Sensitivity and Specificity Translocation, Genetic - genetics Translocation, Genetic - radiation effects |
| title | Monitoring Translocations by M-FISH and Three-color FISH Painting Techniques：A Study of Two Radiotherapy Patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-02T19%3A28%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Monitoring%20Translocations%20by%20M-FISH%20and%20Three-color%20FISH%20Painting%20Techniques%EF%BC%9AA%20Study%20of%20Two%20Radiotherapy%20Patients&rft.jtitle=JOURNAL%20OF%20RADIATION%20RESEARCH&rft.au=Pouzoulet,%20F&rft.aucorp=Rue%20Camille%20Desmoulins&rft.date=2007&rft.volume=48&rft.issue=5&rft.spage=425&rft.epage=434&rft.pages=425-434&rft.issn=0449-3060&rft.eissn=1349-9157&rft_id=info:doi/10.1269/jrr.07013&rft_dat=%3Cproquest_hal_p%3E68315829%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68315829&rft_id=info:pmid/17785937&rfr_iscdi=true |