Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis
In higher plants, cellulose is synthesized at the plasma membrane by the cellulose synthase (CESA) complex. The catalytic core of the complex is believed to be composed of three types of CESA subunits. Indirect evidence suggests that the complex associated with primary wall cellulose deposition cons...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2007-09, Vol.104 (39), p.15566-15571 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Persson, Staffan Paredez, Alexander Carroll, Andrew Palsdottir, Hildur Doblin, Monika Poindexter, Patricia Khitrov, Natalie Auer, Manfred Somerville, Chris R |
| description | In higher plants, cellulose is synthesized at the plasma membrane by the cellulose synthase (CESA) complex. The catalytic core of the complex is believed to be composed of three types of CESA subunits. Indirect evidence suggests that the complex associated with primary wall cellulose deposition consists of CESA1, -3, and -6 in Arabidopsis thaliana. However, phenotypes associated with mutations in two of these genes, CESA1 and -6, suggest unequal contribution by the different CESAs to overall enzymatic activity of the complex. We present evidence that the primary complex requires three unique types of components, CESA1-, CESA3-, and CESA6-related, for activity. Removal of any of these components results in gametophytic lethality due to pollen defects, demonstrating that primary-wall cellulose synthesis is necessary for pollen development. We also show that the CESA6-related CESAs are partially functionally redundant. |
| doi_str_mv | 10.1073/pnas.0706592104 |
| format | Article |
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The catalytic core of the complex is believed to be composed of three types of CESA subunits. Indirect evidence suggests that the complex associated with primary wall cellulose deposition consists of CESA1, -3, and -6 in Arabidopsis thaliana. However, phenotypes associated with mutations in two of these genes, CESA1 and -6, suggest unequal contribution by the different CESAs to overall enzymatic activity of the complex. We present evidence that the primary complex requires three unique types of components, CESA1-, CESA3-, and CESA6-related, for activity. Removal of any of these components results in gametophytic lethality due to pollen defects, demonstrating that primary-wall cellulose synthesis is necessary for pollen development. We also show that the CESA6-related CESAs are partially functionally redundant.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0706592104</identifier><identifier>PMID: 17878302</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis thaliana ; Biological Sciences ; carbohydrate metabolism ; Catalysis ; Cell Wall - enzymology ; Cell walls ; Cellulose ; Cellulose - chemistry ; Complementary DNA ; Flowers & plants ; gametogenesis ; gametophytic lethals ; Gene Expression Regulation, Plant ; Genetic mutation ; Genetic Techniques ; Genetics ; Genotype ; Genotype & phenotype ; Glucosyltransferases - chemistry ; hexosyltransferases ; Hypocotyls ; Light ; Microscopy, Electron ; Models, Genetic ; mutants ; Mutation ; Phenotype ; Phenotypes ; plant fertility ; Plant roots ; Plants ; Pollen ; Pollen - metabolism ; pollen defects ; Polymers ; Protein Isoforms ; Seedlings</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2007-09, Vol.104 (39), p.15566-15571</ispartof><rights>Copyright 2007 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Sep 25, 2007</rights><rights>2007 by The National Academy of Sciences of the USA 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-26803a9adae269511711da188add04b8ac7047c0ca177618a983acd865b10a6b3</citedby><cites>FETCH-LOGICAL-c554t-26803a9adae269511711da188add04b8ac7047c0ca177618a983acd865b10a6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/104/39.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25449175$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25449175$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17878302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Persson, Staffan</creatorcontrib><creatorcontrib>Paredez, Alexander</creatorcontrib><creatorcontrib>Carroll, Andrew</creatorcontrib><creatorcontrib>Palsdottir, Hildur</creatorcontrib><creatorcontrib>Doblin, Monika</creatorcontrib><creatorcontrib>Poindexter, Patricia</creatorcontrib><creatorcontrib>Khitrov, Natalie</creatorcontrib><creatorcontrib>Auer, Manfred</creatorcontrib><creatorcontrib>Somerville, Chris R</creatorcontrib><title>Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>In higher plants, cellulose is synthesized at the plasma membrane by the cellulose synthase (CESA) complex. The catalytic core of the complex is believed to be composed of three types of CESA subunits. Indirect evidence suggests that the complex associated with primary wall cellulose deposition consists of CESA1, -3, and -6 in Arabidopsis thaliana. However, phenotypes associated with mutations in two of these genes, CESA1 and -6, suggest unequal contribution by the different CESAs to overall enzymatic activity of the complex. We present evidence that the primary complex requires three unique types of components, CESA1-, CESA3-, and CESA6-related, for activity. Removal of any of these components results in gametophytic lethality due to pollen defects, demonstrating that primary-wall cellulose synthesis is necessary for pollen development. We also show that the CESA6-related CESAs are partially functionally redundant.</description><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Biological Sciences</subject><subject>carbohydrate metabolism</subject><subject>Catalysis</subject><subject>Cell Wall - enzymology</subject><subject>Cell walls</subject><subject>Cellulose</subject><subject>Cellulose - chemistry</subject><subject>Complementary DNA</subject><subject>Flowers & plants</subject><subject>gametogenesis</subject><subject>gametophytic lethals</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genetic mutation</subject><subject>Genetic Techniques</subject><subject>Genetics</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Glucosyltransferases - chemistry</subject><subject>hexosyltransferases</subject><subject>Hypocotyls</subject><subject>Light</subject><subject>Microscopy, Electron</subject><subject>Models, Genetic</subject><subject>mutants</subject><subject>Mutation</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>plant fertility</subject><subject>Plant roots</subject><subject>Plants</subject><subject>Pollen</subject><subject>Pollen - metabolism</subject><subject>pollen defects</subject><subject>Polymers</subject><subject>Protein Isoforms</subject><subject>Seedlings</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxSMEokvhzAmIOCBxSDt2_HlBqiooSJU4QM_WxPF2vcrai52U9r_H2426wKUnW5rfe5p5r6peEzghINvTbcB8AhIE15QAe1ItCGjSCKbhabUAoLJRjLKj6kXOawDQXMHz6ohIJVULdFFtLlxwo7e1u_G9C9bVy5jqcZWcq6fgf02utnGzjcGFMdc-1NvkN5juauuGofmNw3D_m4aYXZ3vwrjCvJcM7tbdK84Sdr6P2-zzy-rZEofsXs3vcXX15fPP86_N5feLb-dnl43lnI0NFQpa1Nijo0JzQiQhPRKlsO-BdQqtBCYtWCRSCqJQqxZtrwTvCKDo2uPq0953O3Ub19uyfMLBzLubiN78Owl-Za7jjaElI05FMfgwG6RYMsij2fi8OxSDi1M2QrWEM00eBSlQRaXWBXz_H7iOUwolhcIQVspkvECne8immHNyy4eVCZhd4WZXuDkUXhRv_770wM8NF-DjDOyUBztmWm0I50KY5TQMo7sdC1s_whbkzR5Z5zGmB4ZyVtKQuwve7edLjAavk8_m6kc5sAVQBDhp2z87y9Qx</recordid><startdate>20070925</startdate><enddate>20070925</enddate><creator>Persson, Staffan</creator><creator>Paredez, Alexander</creator><creator>Carroll, Andrew</creator><creator>Palsdottir, Hildur</creator><creator>Doblin, Monika</creator><creator>Poindexter, Patricia</creator><creator>Khitrov, Natalie</creator><creator>Auer, Manfred</creator><creator>Somerville, Chris R</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070925</creationdate><title>Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis</title><author>Persson, Staffan ; 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The catalytic core of the complex is believed to be composed of three types of CESA subunits. Indirect evidence suggests that the complex associated with primary wall cellulose deposition consists of CESA1, -3, and -6 in Arabidopsis thaliana. However, phenotypes associated with mutations in two of these genes, CESA1 and -6, suggest unequal contribution by the different CESAs to overall enzymatic activity of the complex. We present evidence that the primary complex requires three unique types of components, CESA1-, CESA3-, and CESA6-related, for activity. Removal of any of these components results in gametophytic lethality due to pollen defects, demonstrating that primary-wall cellulose synthesis is necessary for pollen development. We also show that the CESA6-related CESAs are partially functionally redundant.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>17878302</pmid><doi>10.1073/pnas.0706592104</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis thaliana Biological Sciences carbohydrate metabolism Catalysis Cell Wall - enzymology Cell walls Cellulose Cellulose - chemistry Complementary DNA Flowers & plants gametogenesis gametophytic lethals Gene Expression Regulation, Plant Genetic mutation Genetic Techniques Genetics Genotype Genotype & phenotype Glucosyltransferases - chemistry hexosyltransferases Hypocotyls Light Microscopy, Electron Models, Genetic mutants Mutation Phenotype Phenotypes plant fertility Plant roots Plants Pollen Pollen - metabolism pollen defects Polymers Protein Isoforms Seedlings |
| title | Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis |
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