Using strong injection solvents with 100% aqueous mobile phase in RP-LC
When samples are dissolved for HPLC analysis, organic solvents are often used to enhance the solubility of the sample components. However, when the diluent becomes significantly stronger than the mobile phase, peak shape distortions may develop during injection. This is a serious problem for weakly...
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| Veröffentlicht in: | Journal of separation science 2006-12, Vol.29 (18), p.2847-2852 |
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| container_title | Journal of separation science |
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| creator | Loeser, Eric Drumm, Patrick |
| description | When samples are dissolved for HPLC analysis, organic solvents are often used to enhance the solubility of the sample components. However, when the diluent becomes significantly stronger than the mobile phase, peak shape distortions may develop during injection. This is a serious problem for weakly retained analytes under 100% aqueous mobile phase conditions. Under these extreme conditions, even small amounts of solvent in the diluent can cause serious broadening or distortion effects. However, if the organic solvent used in the diluent elutes significantly after the analyte, the peak shape of the analyte will be relatively unaffected, even when a relatively strong solvent such as THF is used. This phenomenon is counterintuitive considering the usual practice of keeping the sample diluent as weak as possible. This report describes the potential analytical utility and limitations of this approach for the analysis of weakly retained analytes. |
| doi_str_mv | 10.1002/jssc.200600299 |
| format | Article |
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This report describes the potential analytical utility and limitations of this approach for the analysis of weakly retained analytes.</description><subject>Analytical chemistry</subject><subject>Band broadening</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Diluent</subject><subject>Exact sciences and technology</subject><subject>Injection effects</subject><subject>Other chromatographic methods</subject><subject>Peak shape abnormalities</subject><subject>Solvent strength</subject><subject>Solvents - chemistry</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPAjEUhRuj8YFuXZrZ4G6wD_paGlCU4COi0V1TSpHiMINzB5F_bwkE3bnp7U2-c-7JQeiU4AbBmF5MAFyDYiziovUOOiSC8FQz0tzd_rE4QEcAE4yJVBrvowMiGea0KQ9R5wVC_p5AVRZxhHziXRWKPIEi-_J5BckiVOMknqon9nPuizkk02IQMp_MxhZ8VCRPj2mvdYz2RjYDf7KZNfRyffXcukl7D53b1mUvdU1CdOoHQlqOBVWcecetii8dculFTCziIjkRUhOKFSHWMjtU3hLadN6PFKZDVkPna99ZWcQ8UJlpAOezzOarcEaoaKSpimBjDbqyACj9yMzKMLXl0hBsVtWZVXVmW10UnG2c54OpH_7im64iUN8AFpzNRqXNXYBfTjHJFF8Z6TW3iDUt_zlruv1-62-IdK0NUPnvrdaWH0ZIJrl5ve-Yrn6-a7-RrmmzH8e8lVU</recordid><startdate>200612</startdate><enddate>200612</enddate><creator>Loeser, Eric</creator><creator>Drumm, Patrick</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200612</creationdate><title>Using strong injection solvents with 100% aqueous mobile phase in RP-LC</title><author>Loeser, Eric ; Drumm, Patrick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4119-eb67a5062853ec5a83ec2d57e631463ec751679120811aa3ad8ea124ceef802d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analytical chemistry</topic><topic>Band broadening</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Diluent</topic><topic>Exact sciences and technology</topic><topic>Injection effects</topic><topic>Other chromatographic methods</topic><topic>Peak shape abnormalities</topic><topic>Solvent strength</topic><topic>Solvents - chemistry</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loeser, Eric</creatorcontrib><creatorcontrib>Drumm, Patrick</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loeser, Eric</au><au>Drumm, Patrick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Using strong injection solvents with 100% aqueous mobile phase in RP-LC</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Analytical chemistry Band broadening Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Diluent Exact sciences and technology Injection effects Other chromatographic methods Peak shape abnormalities Solvent strength Solvents - chemistry Spectrophotometry, Ultraviolet |
| title | Using strong injection solvents with 100% aqueous mobile phase in RP-LC |
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