Molecular indices of apoptosis activation in elastase-induced aneurysms after embolization with platinum coils
Even though endovascular coils have been widely used for the treatment of intracranial aneurysms, the cellular and molecular responses of aneurysms to the coils after embolization remain poorly understood. The aim of the present study was to understand the mechanism of apoptosis in aneurysms emboliz...
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| Veröffentlicht in: | Stroke (1970) 2007-10, Vol.38 (10), p.2787-2794 |
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| creator | KADIRVEL, Ramanathan DING, Yong-Hong DAYING DAI LEWIS, Debra A CLOFT, Harry J KALLMES, David F |
| description | Even though endovascular coils have been widely used for the treatment of intracranial aneurysms, the cellular and molecular responses of aneurysms to the coils after embolization remain poorly understood. The aim of the present study was to understand the mechanism of apoptosis in aneurysms embolized with platinum coils in the rabbit model of elastase-induced aneurysms.
Elastase-induced saccular aneurysms were created at the origin of the right common carotid artery in 30 rabbits. Aneurysms were allowed to mature for 8 weeks, after which 20 aneurysms were embolized with platinum coils by endovascular means. After 2 (n=10) and 4 (n=10) weeks of implantation, aneurysm samples harboring coils were harvested for apoptotic studies. The remaining 10 uncoiled aneurysms were used as controls; additional controls included the left common carotid artery, which had not undergone any surgical procedure. Control samples were harvested at 12 weeks after aneurysm creation.
Expression of procaspases-3, -8, and -9 was elevated in coiled aneurysms embolized with platinum coils at both time points when compared with uncoiled aneurysms and the left common carotid artery. Cleaved caspases-3, -8, and -9 were found to be expressed only at 4 weeks after embolization. Cells within the aneurysm cavity were terminal dUTP nick end-labeling-positive at 4 weeks only. These apoptotic cells were identified as smooth muscle actin-positive cells. Expression of tumor necrosis-alpha was high in coiled aneurysms when compared with controls. There was no significant difference in the expression of Fas ligand among groups. Decreased expression of antiapoptotic proteins Bcl-2 and phospho-Bad, as well as increased expression of proapoptotic proteins Bax and Bid, was observed in coiled aneurysms at both time points.
Activation of apoptosis in aneurysms after embolization with platinum coils is induced by both tumor necrosis factor-alpha-mediated extrinsic and Bcl-2-mediated intrinsic pathways. |
| doi_str_mv | 10.1161/STROKEAHA.107.486738 |
| format | Article |
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Elastase-induced saccular aneurysms were created at the origin of the right common carotid artery in 30 rabbits. Aneurysms were allowed to mature for 8 weeks, after which 20 aneurysms were embolized with platinum coils by endovascular means. After 2 (n=10) and 4 (n=10) weeks of implantation, aneurysm samples harboring coils were harvested for apoptotic studies. The remaining 10 uncoiled aneurysms were used as controls; additional controls included the left common carotid artery, which had not undergone any surgical procedure. Control samples were harvested at 12 weeks after aneurysm creation.
Expression of procaspases-3, -8, and -9 was elevated in coiled aneurysms embolized with platinum coils at both time points when compared with uncoiled aneurysms and the left common carotid artery. Cleaved caspases-3, -8, and -9 were found to be expressed only at 4 weeks after embolization. Cells within the aneurysm cavity were terminal dUTP nick end-labeling-positive at 4 weeks only. These apoptotic cells were identified as smooth muscle actin-positive cells. Expression of tumor necrosis-alpha was high in coiled aneurysms when compared with controls. There was no significant difference in the expression of Fas ligand among groups. Decreased expression of antiapoptotic proteins Bcl-2 and phospho-Bad, as well as increased expression of proapoptotic proteins Bax and Bid, was observed in coiled aneurysms at both time points.
Activation of apoptosis in aneurysms after embolization with platinum coils is induced by both tumor necrosis factor-alpha-mediated extrinsic and Bcl-2-mediated intrinsic pathways.</description><identifier>ISSN: 0039-2499</identifier><identifier>EISSN: 1524-4628</identifier><identifier>DOI: 10.1161/STROKEAHA.107.486738</identifier><identifier>PMID: 17717314</identifier><identifier>CODEN: SJCCA7</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Animals ; Apoptosis - physiology ; Biological and medical sciences ; Carotid Artery, Common - pathology ; Caspase 3 - metabolism ; Caspase 8 - metabolism ; Caspase 9 - metabolism ; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges ; Disease Models, Animal ; Diseases of the nervous system ; Embolization, Therapeutic - instrumentation ; Embolization, Therapeutic - methods ; Fibroblasts - metabolism ; Fibroblasts - pathology ; Fundamental and applied biological sciences. Psychology ; In Situ Nick-End Labeling ; Intracranial Aneurysm - metabolism ; Intracranial Aneurysm - pathology ; Intracranial Aneurysm - therapy ; Medical sciences ; Neurology ; Pancreatic Elastase ; Platinum ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Rabbits ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Tumor Necrosis Factor-alpha - metabolism ; Vascular diseases and vascular malformations of the nervous system ; Vertebrates: nervous system and sense organs</subject><ispartof>Stroke (1970), 2007-10, Vol.38 (10), p.2787-2794</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-f0b5797b1e8b1e8504b80a708ff940de09f55b3f75f520ea52f85eecc63927b13</citedby><cites>FETCH-LOGICAL-c418t-f0b5797b1e8b1e8504b80a708ff940de09f55b3f75f520ea52f85eecc63927b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,3676,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19127679$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17717314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KADIRVEL, Ramanathan</creatorcontrib><creatorcontrib>DING, Yong-Hong</creatorcontrib><creatorcontrib>DAYING DAI</creatorcontrib><creatorcontrib>LEWIS, Debra A</creatorcontrib><creatorcontrib>CLOFT, Harry J</creatorcontrib><creatorcontrib>KALLMES, David F</creatorcontrib><title>Molecular indices of apoptosis activation in elastase-induced aneurysms after embolization with platinum coils</title><title>Stroke (1970)</title><addtitle>Stroke</addtitle><description>Even though endovascular coils have been widely used for the treatment of intracranial aneurysms, the cellular and molecular responses of aneurysms to the coils after embolization remain poorly understood. The aim of the present study was to understand the mechanism of apoptosis in aneurysms embolized with platinum coils in the rabbit model of elastase-induced aneurysms.
Elastase-induced saccular aneurysms were created at the origin of the right common carotid artery in 30 rabbits. Aneurysms were allowed to mature for 8 weeks, after which 20 aneurysms were embolized with platinum coils by endovascular means. After 2 (n=10) and 4 (n=10) weeks of implantation, aneurysm samples harboring coils were harvested for apoptotic studies. The remaining 10 uncoiled aneurysms were used as controls; additional controls included the left common carotid artery, which had not undergone any surgical procedure. Control samples were harvested at 12 weeks after aneurysm creation.
Expression of procaspases-3, -8, and -9 was elevated in coiled aneurysms embolized with platinum coils at both time points when compared with uncoiled aneurysms and the left common carotid artery. Cleaved caspases-3, -8, and -9 were found to be expressed only at 4 weeks after embolization. Cells within the aneurysm cavity were terminal dUTP nick end-labeling-positive at 4 weeks only. These apoptotic cells were identified as smooth muscle actin-positive cells. Expression of tumor necrosis-alpha was high in coiled aneurysms when compared with controls. There was no significant difference in the expression of Fas ligand among groups. Decreased expression of antiapoptotic proteins Bcl-2 and phospho-Bad, as well as increased expression of proapoptotic proteins Bax and Bid, was observed in coiled aneurysms at both time points.
Activation of apoptosis in aneurysms after embolization with platinum coils is induced by both tumor necrosis factor-alpha-mediated extrinsic and Bcl-2-mediated intrinsic pathways.</description><subject>Animals</subject><subject>Apoptosis - physiology</subject><subject>Biological and medical sciences</subject><subject>Carotid Artery, Common - pathology</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase 8 - metabolism</subject><subject>Caspase 9 - metabolism</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Disease Models, Animal</subject><subject>Diseases of the nervous system</subject><subject>Embolization, Therapeutic - instrumentation</subject><subject>Embolization, Therapeutic - methods</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - pathology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Situ Nick-End Labeling</subject><subject>Intracranial Aneurysm - metabolism</subject><subject>Intracranial Aneurysm - pathology</subject><subject>Intracranial Aneurysm - therapy</subject><subject>Medical sciences</subject><subject>Neurology</subject><subject>Pancreatic Elastase</subject><subject>Platinum</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Rabbits</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Vascular diseases and vascular malformations of the nervous system</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0039-2499</issn><issn>1524-4628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFFvFCEQx4nR2PP0GxjDi33bExZY4PHSVGusadLW5w3LDRHDLiuzq6mfvjR3sQ-EMPz-M5MfIe8523He8U9397c33y73V_sdZ3onTaeFeUE2XLWykV1rXpINY8I2rbT2jLxB_MUYa4VRr8kZ15prweWGTN9zAr8mV2icDtED0hyom_O8ZIxInV_iH7fEPNV_Csnh4hCayq4eDtRNsJYHHCsYFigUxiGn-O8Y-BuXn3RO9TGtI_U5JnxLXgWXEN6d7i358fny_uKqub758vVif914yc3SBDYobfXAwTwdxeRgmNPMhGAlOwCzQalBBK2Cahk41QajALzvhG1rTGzJ-bHvXPLvFXDpx4geUqoL5xX7zgje6qpjS-QR9CUjFgj9XOLoykPPWf_kuf_vuVZ0f_RcYx9O_ddhhMNz6CS2Ah9PgEPvUihu8hGfOVvHd9qKR9AxiXU</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>KADIRVEL, Ramanathan</creator><creator>DING, Yong-Hong</creator><creator>DAYING DAI</creator><creator>LEWIS, Debra A</creator><creator>CLOFT, Harry J</creator><creator>KALLMES, David F</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>Molecular indices of apoptosis activation in elastase-induced aneurysms after embolization with platinum coils</title><author>KADIRVEL, Ramanathan ; DING, Yong-Hong ; DAYING DAI ; LEWIS, Debra A ; CLOFT, Harry J ; KALLMES, David F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-f0b5797b1e8b1e8504b80a708ff940de09f55b3f75f520ea52f85eecc63927b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Apoptosis - physiology</topic><topic>Biological and medical sciences</topic><topic>Carotid Artery, Common - pathology</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase 8 - metabolism</topic><topic>Caspase 9 - metabolism</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Disease Models, Animal</topic><topic>Diseases of the nervous system</topic><topic>Embolization, Therapeutic - instrumentation</topic><topic>Embolization, Therapeutic - methods</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - pathology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Situ Nick-End Labeling</topic><topic>Intracranial Aneurysm - metabolism</topic><topic>Intracranial Aneurysm - pathology</topic><topic>Intracranial Aneurysm - therapy</topic><topic>Medical sciences</topic><topic>Neurology</topic><topic>Pancreatic Elastase</topic><topic>Platinum</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Rabbits</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Vascular diseases and vascular malformations of the nervous system</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KADIRVEL, Ramanathan</creatorcontrib><creatorcontrib>DING, Yong-Hong</creatorcontrib><creatorcontrib>DAYING DAI</creatorcontrib><creatorcontrib>LEWIS, Debra A</creatorcontrib><creatorcontrib>CLOFT, Harry J</creatorcontrib><creatorcontrib>KALLMES, David F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Stroke (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KADIRVEL, Ramanathan</au><au>DING, Yong-Hong</au><au>DAYING DAI</au><au>LEWIS, Debra A</au><au>CLOFT, Harry J</au><au>KALLMES, David F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular indices of apoptosis activation in elastase-induced aneurysms after embolization with platinum coils</atitle><jtitle>Stroke (1970)</jtitle><addtitle>Stroke</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>38</volume><issue>10</issue><spage>2787</spage><epage>2794</epage><pages>2787-2794</pages><issn>0039-2499</issn><eissn>1524-4628</eissn><coden>SJCCA7</coden><abstract>Even though endovascular coils have been widely used for the treatment of intracranial aneurysms, the cellular and molecular responses of aneurysms to the coils after embolization remain poorly understood. The aim of the present study was to understand the mechanism of apoptosis in aneurysms embolized with platinum coils in the rabbit model of elastase-induced aneurysms.
Elastase-induced saccular aneurysms were created at the origin of the right common carotid artery in 30 rabbits. Aneurysms were allowed to mature for 8 weeks, after which 20 aneurysms were embolized with platinum coils by endovascular means. After 2 (n=10) and 4 (n=10) weeks of implantation, aneurysm samples harboring coils were harvested for apoptotic studies. The remaining 10 uncoiled aneurysms were used as controls; additional controls included the left common carotid artery, which had not undergone any surgical procedure. Control samples were harvested at 12 weeks after aneurysm creation.
Expression of procaspases-3, -8, and -9 was elevated in coiled aneurysms embolized with platinum coils at both time points when compared with uncoiled aneurysms and the left common carotid artery. Cleaved caspases-3, -8, and -9 were found to be expressed only at 4 weeks after embolization. Cells within the aneurysm cavity were terminal dUTP nick end-labeling-positive at 4 weeks only. These apoptotic cells were identified as smooth muscle actin-positive cells. Expression of tumor necrosis-alpha was high in coiled aneurysms when compared with controls. There was no significant difference in the expression of Fas ligand among groups. Decreased expression of antiapoptotic proteins Bcl-2 and phospho-Bad, as well as increased expression of proapoptotic proteins Bax and Bid, was observed in coiled aneurysms at both time points.
Activation of apoptosis in aneurysms after embolization with platinum coils is induced by both tumor necrosis factor-alpha-mediated extrinsic and Bcl-2-mediated intrinsic pathways.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>17717314</pmid><doi>10.1161/STROKEAHA.107.486738</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; American Heart Association Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Journals@Ovid Complete; Alma/SFX Local Collection |
| subjects | Animals Apoptosis - physiology Biological and medical sciences Carotid Artery, Common - pathology Caspase 3 - metabolism Caspase 8 - metabolism Caspase 9 - metabolism Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Disease Models, Animal Diseases of the nervous system Embolization, Therapeutic - instrumentation Embolization, Therapeutic - methods Fibroblasts - metabolism Fibroblasts - pathology Fundamental and applied biological sciences. Psychology In Situ Nick-End Labeling Intracranial Aneurysm - metabolism Intracranial Aneurysm - pathology Intracranial Aneurysm - therapy Medical sciences Neurology Pancreatic Elastase Platinum Proto-Oncogene Proteins c-bcl-2 - metabolism Rabbits Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Tumor Necrosis Factor-alpha - metabolism Vascular diseases and vascular malformations of the nervous system Vertebrates: nervous system and sense organs |
| title | Molecular indices of apoptosis activation in elastase-induced aneurysms after embolization with platinum coils |
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