Expression analysis of proteases of Mycobacterium leprae in human skin lesions
Proteases are commonly involved in bacterial pathogenesis and their inhibition has represented a successful therapeutic approach to treat infectious diseases. However, there is little information on the role of proteases in the pathogenesis of Mycobacteria. Five of these genes, three coding for puta...
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| Veröffentlicht in: | Microbial pathogenesis 2007-11, Vol.43 (5), p.249-254 |
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| creator | Ribeiro-Guimarães, Michelle Lopes Tempone, Antonio Jorge Amaral, Julio Jablonski Nery, Jose Augusto Gomes Antunes, Sergio Luiz Pessolani, Maria Cristina Vidal |
| description | Proteases are commonly involved in bacterial pathogenesis and their inhibition has represented a successful therapeutic approach to treat infectious diseases. However, there is little information on the role of proteases in the pathogenesis of Mycobacteria. Five of these genes, three coding for putative secreted proteases, were selected in the present study to investigate their expression in
Mycobacterium leprae isolated from skin biopsies of multibacillary leprosy patients. Via nested-PCR, it was demonstrated that
mycP1 or ML0041,
htrA2 or ML0176,
htrA4 or ML2659,
gcp or ML0379 and
clpC or ML0235 are transcribed in vivo during the course of human infection. Moreover, the expression of Gcp in leprosy lesions was further confirmed by immunohistochemistry using a specific hyperimmune serum. This observation reinforces the potential role of mycobacterial proteases in the context of leprosy pathogenesis. |
| doi_str_mv | 10.1016/j.micpath.2007.05.011 |
| format | Article |
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Mycobacterium leprae isolated from skin biopsies of multibacillary leprosy patients. Via nested-PCR, it was demonstrated that
mycP1 or ML0041,
htrA2 or ML0176,
htrA4 or ML2659,
gcp or ML0379 and
clpC or ML0235 are transcribed in vivo during the course of human infection. Moreover, the expression of Gcp in leprosy lesions was further confirmed by immunohistochemistry using a specific hyperimmune serum. This observation reinforces the potential role of mycobacterial proteases in the context of leprosy pathogenesis.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2007.05.011</identifier><identifier>PMID: 17624714</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Oxford: Elsevier India Pvt Ltd</publisher><subject>Animals ; Antibodies, Bacterial ; Antigens, Bacterial - metabolism ; Bacterial diseases ; Bacteriology ; Biological and medical sciences ; Expression ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial - genetics ; Gene Expression Regulation, Bacterial - physiology ; Human bacterial diseases ; Humans ; Infectious diseases ; Leprosy ; Leprosy - enzymology ; Leprosy - metabolism ; Lesion ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Mycobacterium ; Mycobacterium leprae ; Mycobacterium leprae - enzymology ; Mycobacterium leprae - genetics ; Mycobacterium leprae - pathogenicity ; Peptide Hydrolases - metabolism ; Proteases ; RT-PCR ; Skin - microbiology ; Tropical bacterial diseases</subject><ispartof>Microbial pathogenesis, 2007-11, Vol.43 (5), p.249-254</ispartof><rights>2007 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-18842a479c6b41bd144f5d88c7a12c15e42041aa6041aa609cbdae2ba5d1f4003</citedby><cites>FETCH-LOGICAL-c424t-18842a479c6b41bd144f5d88c7a12c15e42041aa6041aa609cbdae2ba5d1f4003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2007.05.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19121977$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17624714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ribeiro-Guimarães, Michelle Lopes</creatorcontrib><creatorcontrib>Tempone, Antonio Jorge</creatorcontrib><creatorcontrib>Amaral, Julio Jablonski</creatorcontrib><creatorcontrib>Nery, Jose Augusto</creatorcontrib><creatorcontrib>Gomes Antunes, Sergio Luiz</creatorcontrib><creatorcontrib>Pessolani, Maria Cristina Vidal</creatorcontrib><title>Expression analysis of proteases of Mycobacterium leprae in human skin lesions</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Proteases are commonly involved in bacterial pathogenesis and their inhibition has represented a successful therapeutic approach to treat infectious diseases. However, there is little information on the role of proteases in the pathogenesis of Mycobacteria. Five of these genes, three coding for putative secreted proteases, were selected in the present study to investigate their expression in
Mycobacterium leprae isolated from skin biopsies of multibacillary leprosy patients. Via nested-PCR, it was demonstrated that
mycP1 or ML0041,
htrA2 or ML0176,
htrA4 or ML2659,
gcp or ML0379 and
clpC or ML0235 are transcribed in vivo during the course of human infection. Moreover, the expression of Gcp in leprosy lesions was further confirmed by immunohistochemistry using a specific hyperimmune serum. This observation reinforces the potential role of mycobacterial proteases in the context of leprosy pathogenesis.</description><subject>Animals</subject><subject>Antibodies, Bacterial</subject><subject>Antigens, Bacterial - metabolism</subject><subject>Bacterial diseases</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Expression</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial - genetics</subject><subject>Gene Expression Regulation, Bacterial - physiology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Leprosy</subject><subject>Leprosy - enzymology</subject><subject>Leprosy - metabolism</subject><subject>Lesion</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycobacterium</subject><subject>Mycobacterium leprae</subject><subject>Mycobacterium leprae - enzymology</subject><subject>Mycobacterium leprae - genetics</subject><subject>Mycobacterium leprae - pathogenicity</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Proteases</subject><subject>RT-PCR</subject><subject>Skin - microbiology</subject><subject>Tropical bacterial diseases</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlu1EAQQFsIRCaBTwD5Ajebqp72dkIoCgEpwAXOrXK7rPTgjS47Yv6eHsZSjrnUIr1a9JR6g5AhYPHhkA3ezbTcZxqgzCDPAPGZ2iHURYoaqudqB1WlUwMIF-pS5AAAtdnXL9UFloU2JZqd-n7zdw4s4qcxoZH6o3hJpi6Zw7QwCf9vvh3d1JBbOPh1SHqeA3Hix-R-HWhM5Hcsez6tkFfqRUe98OstX6lfn29-Xn9J737cfr3-dJc6o82SYlUZTaasXdEYbFo0psvbqnIloXaYs9FgkKjYYu2allg3lLfYGYD9lXp_3hv__LOyLHbw4rjvaeRpFVtU-ygC8Ekw3tmXkFcRzM-gC5NI4M7OwQ8UjhbBnozbg92M25NxC7mNxuPc2-3A2gzcPk5tiiPwbgNIHPVdoNF5eeRq1FiXZeQ-njmO3h48ByvO8-i49YHdYtvJP_HKP3SsoYQ</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Ribeiro-Guimarães, Michelle Lopes</creator><creator>Tempone, Antonio Jorge</creator><creator>Amaral, Julio Jablonski</creator><creator>Nery, Jose Augusto</creator><creator>Gomes Antunes, Sergio Luiz</creator><creator>Pessolani, Maria Cristina Vidal</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Expression analysis of proteases of Mycobacterium leprae in human skin lesions</title><author>Ribeiro-Guimarães, Michelle Lopes ; Tempone, Antonio Jorge ; Amaral, Julio Jablonski ; Nery, Jose Augusto ; Gomes Antunes, Sergio Luiz ; Pessolani, Maria Cristina Vidal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-18842a479c6b41bd144f5d88c7a12c15e42041aa6041aa609cbdae2ba5d1f4003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial</topic><topic>Antigens, Bacterial - metabolism</topic><topic>Bacterial diseases</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Expression</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial - genetics</topic><topic>Gene Expression Regulation, Bacterial - physiology</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Leprosy</topic><topic>Leprosy - enzymology</topic><topic>Leprosy - metabolism</topic><topic>Lesion</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycobacterium</topic><topic>Mycobacterium leprae</topic><topic>Mycobacterium leprae - enzymology</topic><topic>Mycobacterium leprae - genetics</topic><topic>Mycobacterium leprae - pathogenicity</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Proteases</topic><topic>RT-PCR</topic><topic>Skin - microbiology</topic><topic>Tropical bacterial diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ribeiro-Guimarães, Michelle Lopes</creatorcontrib><creatorcontrib>Tempone, Antonio Jorge</creatorcontrib><creatorcontrib>Amaral, Julio Jablonski</creatorcontrib><creatorcontrib>Nery, Jose Augusto</creatorcontrib><creatorcontrib>Gomes Antunes, Sergio Luiz</creatorcontrib><creatorcontrib>Pessolani, Maria Cristina Vidal</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ribeiro-Guimarães, Michelle Lopes</au><au>Tempone, Antonio Jorge</au><au>Amaral, Julio Jablonski</au><au>Nery, Jose Augusto</au><au>Gomes Antunes, Sergio Luiz</au><au>Pessolani, Maria Cristina Vidal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression analysis of proteases of Mycobacterium leprae in human skin lesions</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>43</volume><issue>5</issue><spage>249</spage><epage>254</epage><pages>249-254</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>Proteases are commonly involved in bacterial pathogenesis and their inhibition has represented a successful therapeutic approach to treat infectious diseases. 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Mycobacterium leprae isolated from skin biopsies of multibacillary leprosy patients. Via nested-PCR, it was demonstrated that
mycP1 or ML0041,
htrA2 or ML0176,
htrA4 or ML2659,
gcp or ML0379 and
clpC or ML0235 are transcribed in vivo during the course of human infection. Moreover, the expression of Gcp in leprosy lesions was further confirmed by immunohistochemistry using a specific hyperimmune serum. This observation reinforces the potential role of mycobacterial proteases in the context of leprosy pathogenesis.</abstract><cop>Oxford</cop><pub>Elsevier India Pvt Ltd</pub><pmid>17624714</pmid><doi>10.1016/j.micpath.2007.05.011</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Antibodies, Bacterial Antigens, Bacterial - metabolism Bacterial diseases Bacteriology Biological and medical sciences Expression Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - genetics Gene Expression Regulation, Bacterial - physiology Human bacterial diseases Humans Infectious diseases Leprosy Leprosy - enzymology Leprosy - metabolism Lesion Medical sciences Mice Mice, Inbred BALB C Microbiology Miscellaneous Mycobacterium Mycobacterium leprae Mycobacterium leprae - enzymology Mycobacterium leprae - genetics Mycobacterium leprae - pathogenicity Peptide Hydrolases - metabolism Proteases RT-PCR Skin - microbiology Tropical bacterial diseases |
| title | Expression analysis of proteases of Mycobacterium leprae in human skin lesions |
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