Dynamic Visualization of Thrombopoiesis Within Bone Marrow
Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic p...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2007-09, Vol.317 (5845), p.1767-1770 |
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| creator | Junt, Tobias Schulze, Harald Chen, Zhao Massberg, Steffen Goerge, Tobias Krueger, Andreas Wagner, Denisa D Graf, Thomas Italiano, Joseph E. Jr Shivdasani, Ramesh A von Andrian, Ulrich H |
| description | Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis. |
| doi_str_mv | 10.1126/science.1146304 |
| format | Article |
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Jr ; Shivdasani, Ramesh A ; von Andrian, Ulrich H</creator><creatorcontrib>Junt, Tobias ; Schulze, Harald ; Chen, Zhao ; Massberg, Steffen ; Goerge, Tobias ; Krueger, Andreas ; Wagner, Denisa D ; Graf, Thomas ; Italiano, Joseph E. Jr ; Shivdasani, Ramesh A ; von Andrian, Ulrich H</creatorcontrib><description>Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1146304</identifier><identifier>PMID: 17885137</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Animals ; Bacterial Proteins ; Biological and medical sciences ; Blood ; Blood Platelets - cytology ; Bone marrow ; Bone Marrow - physiology ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cells, Cultured ; Cellular biology ; Fluid dynamics ; Fundamental and applied biological sciences. Psychology ; Imaging ; Luminescent Proteins ; Megakaryocytes - cytology ; Mice ; Microscopy ; Microscopy, Fluorescence, Multiphoton ; Microvessels ; Molecular and cellular biology ; Platelet Membrane Glycoprotein IIb ; Platelets ; Pseudopodia ; Rodents ; Shear Strength ; Shear stress ; Sine waves ; Swelling ; Thrombopoiesis - physiology</subject><ispartof>Science (American Association for the Advancement of Science), 2007-09, Vol.317 (5845), p.1767-1770</ispartof><rights>Copyright 2007 American Association for the Advancement of Science</rights><rights>2007 INIST-CNRS</rights><rights>Copyright © 2007, American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-6692471642816c7617091ef613fb81949cfa943188d5a2e8c8faa2245c42a64d3</citedby><cites>FETCH-LOGICAL-c526t-6692471642816c7617091ef613fb81949cfa943188d5a2e8c8faa2245c42a64d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20048436$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20048436$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19118871$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17885137$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Junt, Tobias</creatorcontrib><creatorcontrib>Schulze, Harald</creatorcontrib><creatorcontrib>Chen, Zhao</creatorcontrib><creatorcontrib>Massberg, Steffen</creatorcontrib><creatorcontrib>Goerge, Tobias</creatorcontrib><creatorcontrib>Krueger, Andreas</creatorcontrib><creatorcontrib>Wagner, Denisa D</creatorcontrib><creatorcontrib>Graf, Thomas</creatorcontrib><creatorcontrib>Italiano, Joseph E. Jr</creatorcontrib><creatorcontrib>Shivdasani, Ramesh A</creatorcontrib><creatorcontrib>von Andrian, Ulrich H</creatorcontrib><title>Dynamic Visualization of Thrombopoiesis Within Bone Marrow</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.</description><subject>Animals</subject><subject>Bacterial Proteins</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Blood Platelets - cytology</subject><subject>Bone marrow</subject><subject>Bone Marrow - physiology</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Fluid dynamics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Imaging</subject><subject>Luminescent Proteins</subject><subject>Megakaryocytes - cytology</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Microscopy, Fluorescence, Multiphoton</subject><subject>Microvessels</subject><subject>Molecular and cellular biology</subject><subject>Platelet Membrane Glycoprotein IIb</subject><subject>Platelets</subject><subject>Pseudopodia</subject><subject>Rodents</subject><subject>Shear Strength</subject><subject>Shear stress</subject><subject>Sine waves</subject><subject>Swelling</subject><subject>Thrombopoiesis - physiology</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvhzAmIkOAWOuOv2NygtAWpiAMtHC2v16ZeJfHWToTKr8fVRlTi0tNo9D7zSvMQ8hzhHSKVR8VFPzpfFy4Z8AdkhaBFqymwh2QFwGSroBMH5EkpW4CaafaYHGCnlEDWrcj7TzejHaJrfsQy2z7-sVNMY5NCc3GV07BOuxR9iaX5GaerODYf0-ibrzbn9PspeRRsX_yzZR6Sy9OTi-PP7fm3sy_HH85bJ6icWik15R1KThVK10nsQKMPEllYK9Rcu2A1Z6jURljqlVPBWkq5cJxayTfskLzd9-5yup59mcwQi_N9b0ef5mKkYgAdxXtBChxRIFTw9X_gNs15rE-YWiM0cK0rdLSHXE6lZB_MLsfB5huDYG7lm0W-WeTXi5dL7bwe_OaOX2xX4M0C2OJsH7IdXSx3nMZqobt95MWe25Yp5X85BeCKM1nzV_s82GTsr1w7Lr9TwOpBgeIC2F8XOZ4I</recordid><startdate>20070921</startdate><enddate>20070921</enddate><creator>Junt, Tobias</creator><creator>Schulze, Harald</creator><creator>Chen, Zhao</creator><creator>Massberg, Steffen</creator><creator>Goerge, Tobias</creator><creator>Krueger, Andreas</creator><creator>Wagner, Denisa D</creator><creator>Graf, Thomas</creator><creator>Italiano, Joseph E. Jr</creator><creator>Shivdasani, Ramesh A</creator><creator>von Andrian, Ulrich H</creator><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20070921</creationdate><title>Dynamic Visualization of Thrombopoiesis Within Bone Marrow</title><author>Junt, Tobias ; Schulze, Harald ; Chen, Zhao ; Massberg, Steffen ; Goerge, Tobias ; Krueger, Andreas ; Wagner, Denisa D ; Graf, Thomas ; Italiano, Joseph E. Jr ; Shivdasani, Ramesh A ; von Andrian, Ulrich H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-6692471642816c7617091ef613fb81949cfa943188d5a2e8c8faa2245c42a64d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Bacterial Proteins</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Blood Platelets - cytology</topic><topic>Bone marrow</topic><topic>Bone Marrow - physiology</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>Fluid dynamics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Imaging</topic><topic>Luminescent Proteins</topic><topic>Megakaryocytes - cytology</topic><topic>Mice</topic><topic>Microscopy</topic><topic>Microscopy, Fluorescence, Multiphoton</topic><topic>Microvessels</topic><topic>Molecular and cellular biology</topic><topic>Platelet Membrane Glycoprotein IIb</topic><topic>Platelets</topic><topic>Pseudopodia</topic><topic>Rodents</topic><topic>Shear Strength</topic><topic>Shear stress</topic><topic>Sine waves</topic><topic>Swelling</topic><topic>Thrombopoiesis - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Junt, Tobias</creatorcontrib><creatorcontrib>Schulze, Harald</creatorcontrib><creatorcontrib>Chen, Zhao</creatorcontrib><creatorcontrib>Massberg, Steffen</creatorcontrib><creatorcontrib>Goerge, Tobias</creatorcontrib><creatorcontrib>Krueger, Andreas</creatorcontrib><creatorcontrib>Wagner, Denisa D</creatorcontrib><creatorcontrib>Graf, Thomas</creatorcontrib><creatorcontrib>Italiano, Joseph E. 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Jr</au><au>Shivdasani, Ramesh A</au><au>von Andrian, Ulrich H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic Visualization of Thrombopoiesis Within Bone Marrow</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2007-09-21</date><risdate>2007</risdate><volume>317</volume><issue>5845</issue><spage>1767</spage><epage>1770</epage><pages>1767-1770</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>17885137</pmid><doi>10.1126/science.1146304</doi><tpages>4</tpages></addata></record> |
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| ispartof | Science (American Association for the Advancement of Science), 2007-09, Vol.317 (5845), p.1767-1770 |
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| source | Jstor Complete Legacy; MEDLINE; Science Magazine |
| subjects | Animals Bacterial Proteins Biological and medical sciences Blood Blood Platelets - cytology Bone marrow Bone Marrow - physiology Cell differentiation, maturation, development, hematopoiesis Cell physiology Cells, Cultured Cellular biology Fluid dynamics Fundamental and applied biological sciences. Psychology Imaging Luminescent Proteins Megakaryocytes - cytology Mice Microscopy Microscopy, Fluorescence, Multiphoton Microvessels Molecular and cellular biology Platelet Membrane Glycoprotein IIb Platelets Pseudopodia Rodents Shear Strength Shear stress Sine waves Swelling Thrombopoiesis - physiology |
| title | Dynamic Visualization of Thrombopoiesis Within Bone Marrow |
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