Analysis of synaptic ultrastructure without fixative using high-pressure freezing and tomography

Electron microscopy allows the analysis of synaptic ultrastructure and its modifications during learning or in pathological conditions. However, conventional electron microscopy uses aldehyde fixatives that alter the morphology of the synapse by changing osmolarity and collapsing its molecular compo...

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Veröffentlicht in:	The European journal of neuroscience 2006-12, Vol.24 (12), p.3463-3474
Hauptverfasser:	Rostaing, Philippe, Real, Eleonore, Siksou, Léa, Lechaire, Jean-Pierre, Boudier, Thomas, Boeckers, Tobias M., Gertler, Frank, Gundelfinger, Eckart D., Triller, Antoine, Marty, Serge
Format:	Artikel
Sprache:	eng
Schlagworte:	actin cytoskeleton Aldehydes - pharmacology Animals Animals, Newborn Calcium-Calmodulin-Dependent Protein Kinase Type 2 Calcium-Calmodulin-Dependent Protein Kinases - metabolism Cortactin - metabolism Cryopreservation - methods dendritic spines Freezing hippocampus Hippocampus - ultrastructure Microscopy, Electron, Transmission - methods Microscopy, Immunoelectron - methods postsynaptic density rat Rats Receptors, Glutamate - metabolism Synapses - drug effects Synapses - metabolism Synapses - ultrastructure Tissue Fixation - methods Tomography
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