Molecular Analysis of Neurolysin Expression in the Rat and Bovine Ciliary Body
Purpose: This paper deals with the capability of the ciliary epithelium to express neurolysin, involved in the inactivation of numerous neuropeptides. Methods: Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CB...
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| Veröffentlicht in: | Current eye research 2007-01, Vol.32 (9), p.751-756 |
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| container_title | Current eye research |
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| creator | Bertazolli-Filho, Rubens Coca-Prados, Miguel Haddad, Antonio Laicine, Eduardo Miguel |
| description | Purpose: This paper deals with the capability of the ciliary epithelium to express neurolysin, involved in the inactivation of numerous neuropeptides. Methods: Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization. Results: The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium. Conclusions: These results indicate that the ciliary epithelium expresses neurolysin. |
| doi_str_mv | 10.1080/02713680701573381 |
| format | Article |
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Methods: Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization. Results: The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium. Conclusions: These results indicate that the ciliary epithelium expresses neurolysin.</description><identifier>ISSN: 0271-3683</identifier><identifier>EISSN: 1460-2202</identifier><identifier>DOI: 10.1080/02713680701573381</identifier><identifier>PMID: 17882707</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Blotting, Western ; Cattle ; Ciliary Body - cytology ; Ciliary Body - metabolism ; ciliary epithelium ; Cross Reactions ; Epithelial Cells - metabolism ; Epithelium - metabolism ; Immunochemistry ; immunocytochemistry ; Male ; Metalloendopeptidases - genetics ; Metalloendopeptidases - metabolism ; neurolysin ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; RT-PCR ; Tissue Distribution ; Western blot</subject><ispartof>Current eye research, 2007-01, Vol.32 (9), p.751-756</ispartof><rights>2007 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-765956d9e4ebc0349f164f51eb252d943632df6afc9235c1e0258abeec7adf563</citedby><cites>FETCH-LOGICAL-c404t-765956d9e4ebc0349f164f51eb252d943632df6afc9235c1e0258abeec7adf563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/02713680701573381$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/02713680701573381$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17882707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bertazolli-Filho, Rubens</creatorcontrib><creatorcontrib>Coca-Prados, Miguel</creatorcontrib><creatorcontrib>Haddad, Antonio</creatorcontrib><creatorcontrib>Laicine, Eduardo Miguel</creatorcontrib><title>Molecular Analysis of Neurolysin Expression in the Rat and Bovine Ciliary Body</title><title>Current eye research</title><addtitle>Curr Eye Res</addtitle><description>Purpose: This paper deals with the capability of the ciliary epithelium to express neurolysin, involved in the inactivation of numerous neuropeptides. Methods: Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization. Results: The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium. Conclusions: These results indicate that the ciliary epithelium expresses neurolysin.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cattle</subject><subject>Ciliary Body - cytology</subject><subject>Ciliary Body - metabolism</subject><subject>ciliary epithelium</subject><subject>Cross Reactions</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelium - metabolism</subject><subject>Immunochemistry</subject><subject>immunocytochemistry</subject><subject>Male</subject><subject>Metalloendopeptidases - genetics</subject><subject>Metalloendopeptidases - metabolism</subject><subject>neurolysin</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>RT-PCR</subject><subject>Tissue Distribution</subject><subject>Western blot</subject><issn>0271-3683</issn><issn>1460-2202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd1LwzAUxYMobk7_AF8kT75V89GmLfoyh1-gE0SfQ5besIysmUmr7r-3ZQMRYU-Xc_M7h8sJQqeUXFBSkEvCcspFQXJCs5zzgu6hIU0FSRgjbB8N-_ekA_gAHcW4IKRfpIdoQPOiYDnJh2j67B3o1qmAx7Vy62gj9gZPoQ2-VzW-_V4FiNH6GneqmQN-VQ1WdYVv_KetAU-ssyqsO1mtj9GBUS7CyXaO0Pvd7dvkIXl6uX-cjJ8SnZK0SXKRlZmoSkhhpglPS0NFajIKM5axqky54KwyQhldMp5pCoRlhZoB6FxVJhN8hM43uavgP1qIjVzaqME5VYNvoxQFK6kgPUg3oA4-xgBGroJddudKSmRfovxXYuc524a3syVUv45tax1wvQFsbXxYqi8fXCUbtXY-mKBqbaPku_Kv_tjnoFwz1yqAXPg2dN8Qd1z3A8xdkU8</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Bertazolli-Filho, Rubens</creator><creator>Coca-Prados, Miguel</creator><creator>Haddad, Antonio</creator><creator>Laicine, Eduardo Miguel</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Molecular Analysis of Neurolysin Expression in the Rat and Bovine Ciliary Body</title><author>Bertazolli-Filho, Rubens ; Coca-Prados, Miguel ; Haddad, Antonio ; Laicine, Eduardo Miguel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-765956d9e4ebc0349f164f51eb252d943632df6afc9235c1e0258abeec7adf563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cattle</topic><topic>Ciliary Body - cytology</topic><topic>Ciliary Body - metabolism</topic><topic>ciliary epithelium</topic><topic>Cross Reactions</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelium - metabolism</topic><topic>Immunochemistry</topic><topic>immunocytochemistry</topic><topic>Male</topic><topic>Metalloendopeptidases - genetics</topic><topic>Metalloendopeptidases - metabolism</topic><topic>neurolysin</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>RT-PCR</topic><topic>Tissue Distribution</topic><topic>Western blot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bertazolli-Filho, Rubens</creatorcontrib><creatorcontrib>Coca-Prados, Miguel</creatorcontrib><creatorcontrib>Haddad, Antonio</creatorcontrib><creatorcontrib>Laicine, Eduardo Miguel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bertazolli-Filho, Rubens</au><au>Coca-Prados, Miguel</au><au>Haddad, Antonio</au><au>Laicine, Eduardo Miguel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Analysis of Neurolysin Expression in the Rat and Bovine Ciliary Body</atitle><jtitle>Current eye research</jtitle><addtitle>Curr Eye Res</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>32</volume><issue>9</issue><spage>751</spage><epage>756</epage><pages>751-756</pages><issn>0271-3683</issn><eissn>1460-2202</eissn><abstract>Purpose: This paper deals with the capability of the ciliary epithelium to express neurolysin, involved in the inactivation of numerous neuropeptides. Methods: Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization. Results: The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium. Conclusions: These results indicate that the ciliary epithelium expresses neurolysin.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>17882707</pmid><doi>10.1080/02713680701573381</doi><tpages>6</tpages></addata></record> |
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| source | Taylor & Francis; MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | Animals Blotting, Western Cattle Ciliary Body - cytology Ciliary Body - metabolism ciliary epithelium Cross Reactions Epithelial Cells - metabolism Epithelium - metabolism Immunochemistry immunocytochemistry Male Metalloendopeptidases - genetics Metalloendopeptidases - metabolism neurolysin Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism RT-PCR Tissue Distribution Western blot |
| title | Molecular Analysis of Neurolysin Expression in the Rat and Bovine Ciliary Body |
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