Molecular typing by random amplification of polymorphic DNA (RAPD) and detection of virulence genes of Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum
Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum is the causative agent of fowl typhoid in chickens, outbreaks of which have devastated poultry populations in Korea since 1992. In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined u...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2006, Vol.68(12), pp.1321-1326 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | Jin, J.D.(Kangwon National Univ., Chunchon (Korea R.)) Lee, D.S Shin, E.K Kim, S.J Jung, R Hahn, T.W |
| description | Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum is the causative agent of fowl typhoid in chickens, outbreaks of which have devastated poultry populations in Korea since 1992. In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined using the random amplified polymorphic DNA (RAPD) method. Of 13 arbitrary primers screened initially, the primer designated as universal rice primer-6 (URP-6) was selected for subsequent typing assays because it produced a distinctive and reproducible DNA fingerprint for a S. Gallinarum reference strain. URP-6-based RAPD analysis assigned 30 S. Gallinarum isolates into 6 types, with 26 isolates (86.6%) belonging to 2 major RAPD types. The distribution of virulence genes in S. Gallinarum isolates was examined by Southern hybridization. All tested isolates had the invasion gene, invA, the virulence plasmid gene, spvB, and the S. Enteritidis fimbrial gene, sefC. The distribution of virulence genes among S. Gallinarum isolates did not correlate with any specific RAPD type. |
| doi_str_mv | 10.1292/jvms.68.1321 |
| format | Article |
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In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined using the random amplified polymorphic DNA (RAPD) method. Of 13 arbitrary primers screened initially, the primer designated as universal rice primer-6 (URP-6) was selected for subsequent typing assays because it produced a distinctive and reproducible DNA fingerprint for a S. Gallinarum reference strain. URP-6-based RAPD analysis assigned 30 S. Gallinarum isolates into 6 types, with 26 isolates (86.6%) belonging to 2 major RAPD types. The distribution of virulence genes in S. Gallinarum isolates was examined by Southern hybridization. All tested isolates had the invasion gene, invA, the virulence plasmid gene, spvB, and the S. Enteritidis fimbrial gene, sefC. The distribution of virulence genes among S. Gallinarum isolates did not correlate with any specific RAPD type.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.68.1321</identifier><identifier>PMID: 17213701</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Bacterial Typing Techniques - methods ; CHICKENS ; GENE ; GENES ; Genes, Bacterial - genetics ; Genetic Variation ; GENOTIPOS ; GENOTYPE ; GENOTYPES ; Oryza sativa ; PATHOGENICITY ; PATOGENICIDAD ; POLLO ; POULET ; POUVOIR PATHOGENE ; Random Amplified Polymorphic DNA Technique - veterinary ; RAPD ; Salmonella enterica ; Salmonella enterica - classification ; Salmonella enterica - genetics ; Salmonella enterica - pathogenicity ; SALMONELLA GALLINARUM ; SALMONERA GALLINARUM ; universal rice primers ; Virulence - genetics ; Virulence Factors - genetics ; virulence gene</subject><ispartof>Journal of Veterinary Medical Science, 2006, Vol.68(12), pp.1321-1326</ispartof><rights>2006 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-5437ed50e8f92676edd804f8b1fa91282cbf036fefc500310ffd6f9b0ab1bea63</citedby><cites>FETCH-LOGICAL-c691t-5437ed50e8f92676edd804f8b1fa91282cbf036fefc500310ffd6f9b0ab1bea63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17213701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jin, J.D.(Kangwon National Univ., Chunchon (Korea R.))</creatorcontrib><creatorcontrib>Lee, D.S</creatorcontrib><creatorcontrib>Shin, E.K</creatorcontrib><creatorcontrib>Kim, S.J</creatorcontrib><creatorcontrib>Jung, R</creatorcontrib><creatorcontrib>Hahn, T.W</creatorcontrib><title>Molecular typing by random amplification of polymorphic DNA (RAPD) and detection of virulence genes of Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum is the causative agent of fowl typhoid in chickens, outbreaks of which have devastated poultry populations in Korea since 1992. In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined using the random amplified polymorphic DNA (RAPD) method. Of 13 arbitrary primers screened initially, the primer designated as universal rice primer-6 (URP-6) was selected for subsequent typing assays because it produced a distinctive and reproducible DNA fingerprint for a S. Gallinarum reference strain. URP-6-based RAPD analysis assigned 30 S. Gallinarum isolates into 6 types, with 26 isolates (86.6%) belonging to 2 major RAPD types. The distribution of virulence genes in S. Gallinarum isolates was examined by Southern hybridization. All tested isolates had the invasion gene, invA, the virulence plasmid gene, spvB, and the S. Enteritidis fimbrial gene, sefC. The distribution of virulence genes among S. Gallinarum isolates did not correlate with any specific RAPD type.</description><subject>Bacterial Typing Techniques - methods</subject><subject>CHICKENS</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetic Variation</subject><subject>GENOTIPOS</subject><subject>GENOTYPE</subject><subject>GENOTYPES</subject><subject>Oryza sativa</subject><subject>PATHOGENICITY</subject><subject>PATOGENICIDAD</subject><subject>POLLO</subject><subject>POULET</subject><subject>POUVOIR PATHOGENE</subject><subject>Random Amplified Polymorphic DNA Technique - veterinary</subject><subject>RAPD</subject><subject>Salmonella enterica</subject><subject>Salmonella enterica - classification</subject><subject>Salmonella enterica - genetics</subject><subject>Salmonella enterica - pathogenicity</subject><subject>SALMONELLA GALLINARUM</subject><subject>SALMONERA GALLINARUM</subject><subject>universal rice primers</subject><subject>Virulence - genetics</subject><subject>Virulence Factors - genetics</subject><subject>virulence gene</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl2LEzEUhgdR3Lp6560SEETB1nxMJ8ll6eqqrLr4cT1kMifdlEwyJjOF_i7_oBlat7A3epPAm4eHc8hbFE8JXhAq6dvtrkuLSiwIo-ReMSOs5HNeMnm_mGFJqjmnS3xWPEppizElZSUfFmeEU8I4JrPi9-fgQI9ORTTse-s3qNmjqHwbOqS63lljtRps8CgY1Ae370Lsb6xGF19W6NW31fXFa5Rp1MIA-i-3s3F04DWgDXhIU_RduS54cE4h8APEbEVpbFIP2mbilEEMuzzMpXLOehXHDjX2TvK4eGCUS_DkeJ8XP9-_-7H-ML_6evlxvbqa60qSYb4sGYd2iUEYSSteQdsKXBrREKMkoYLqxmBWGTB6iTEj2Ji2MrLBqiENqIqdFy8P3j6GXyOkoe5s0tMOHsKY6kpQIUpO_wlSTJZcVOX_gAwzyjL44g64DWP0eds6f6HgmAsuM_XmQOkYUopg6j7aTsV9TXA9laOeypHnrKdyZPz5UTo2HbQn-NiGDKwPwDYNagO3gIqD1Q5ONno8J-3tq75RsQafLc8OFqNCrTbRpvrTNcWYY1xKSdgfpGvZpQ</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Jin, J.D.(Kangwon National Univ., Chunchon (Korea R.))</creator><creator>Lee, D.S</creator><creator>Shin, E.K</creator><creator>Kim, S.J</creator><creator>Jung, R</creator><creator>Hahn, T.W</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Molecular typing by random amplification of polymorphic DNA (RAPD) and detection of virulence genes of Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum</title><author>Jin, J.D.(Kangwon National Univ., Chunchon (Korea R.)) ; Lee, D.S ; Shin, E.K ; Kim, S.J ; Jung, R ; Hahn, T.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c691t-5437ed50e8f92676edd804f8b1fa91282cbf036fefc500310ffd6f9b0ab1bea63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacterial Typing Techniques - methods</topic><topic>CHICKENS</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetic Variation</topic><topic>GENOTIPOS</topic><topic>GENOTYPE</topic><topic>GENOTYPES</topic><topic>Oryza sativa</topic><topic>PATHOGENICITY</topic><topic>PATOGENICIDAD</topic><topic>POLLO</topic><topic>POULET</topic><topic>POUVOIR PATHOGENE</topic><topic>Random Amplified Polymorphic DNA Technique - veterinary</topic><topic>RAPD</topic><topic>Salmonella enterica</topic><topic>Salmonella enterica - classification</topic><topic>Salmonella enterica - genetics</topic><topic>Salmonella enterica - pathogenicity</topic><topic>SALMONELLA GALLINARUM</topic><topic>SALMONERA GALLINARUM</topic><topic>universal rice primers</topic><topic>Virulence - genetics</topic><topic>Virulence Factors - genetics</topic><topic>virulence gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, J.D.(Kangwon National Univ., Chunchon (Korea R.))</creatorcontrib><creatorcontrib>Lee, D.S</creatorcontrib><creatorcontrib>Shin, E.K</creatorcontrib><creatorcontrib>Kim, S.J</creatorcontrib><creatorcontrib>Jung, R</creatorcontrib><creatorcontrib>Hahn, T.W</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, J.D.(Kangwon National Univ., Chunchon (Korea R.))</au><au>Lee, D.S</au><au>Shin, E.K</au><au>Kim, S.J</au><au>Jung, R</au><au>Hahn, T.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular typing by random amplification of polymorphic DNA (RAPD) and detection of virulence genes of Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>68</volume><issue>12</issue><spage>1321</spage><epage>1326</epage><pages>1321-1326</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum is the causative agent of fowl typhoid in chickens, outbreaks of which have devastated poultry populations in Korea since 1992. In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined using the random amplified polymorphic DNA (RAPD) method. Of 13 arbitrary primers screened initially, the primer designated as universal rice primer-6 (URP-6) was selected for subsequent typing assays because it produced a distinctive and reproducible DNA fingerprint for a S. Gallinarum reference strain. URP-6-based RAPD analysis assigned 30 S. Gallinarum isolates into 6 types, with 26 isolates (86.6%) belonging to 2 major RAPD types. The distribution of virulence genes in S. Gallinarum isolates was examined by Southern hybridization. All tested isolates had the invasion gene, invA, the virulence plasmid gene, spvB, and the S. Enteritidis fimbrial gene, sefC. The distribution of virulence genes among S. Gallinarum isolates did not correlate with any specific RAPD type.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>17213701</pmid><doi>10.1292/jvms.68.1321</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Veterinary Medical Science, 2006, Vol.68(12), pp.1321-1326 |
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| subjects | Bacterial Typing Techniques - methods CHICKENS GENE GENES Genes, Bacterial - genetics Genetic Variation GENOTIPOS GENOTYPE GENOTYPES Oryza sativa PATHOGENICITY PATOGENICIDAD POLLO POULET POUVOIR PATHOGENE Random Amplified Polymorphic DNA Technique - veterinary RAPD Salmonella enterica Salmonella enterica - classification Salmonella enterica - genetics Salmonella enterica - pathogenicity SALMONELLA GALLINARUM SALMONERA GALLINARUM universal rice primers Virulence - genetics Virulence Factors - genetics virulence gene |
| title | Molecular typing by random amplification of polymorphic DNA (RAPD) and detection of virulence genes of Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum |
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