Homologous peptide of connective tissue growth factor ameliorates epithelial to mesenchymal transition of tubular epithelial cells
The hallmark of failing renal transplants is tubular atrophy and interstitial fibrosis. The cytokine connective tissue growth factor (CTGF or CCN2) plays an important role in epithelial–mesenchymal transition (EMT) of tubular epithelial cells (TECs). A unique domain within CTGF (IRTPKISKPIKFELSG) wh...
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| Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 2006-10, Vol.36 (1), p.35-44 |
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| creator | Shi, Yujun Tu, Zhidan Wang, Wei Li, Qing Ye, Feng Wang, Jinjing Qiu, Jing Zhang, Li Bu, Hong Li, Youping |
| description | The hallmark of failing renal transplants is tubular atrophy and interstitial fibrosis. The cytokine connective tissue growth factor (CTGF or CCN2) plays an important role in epithelial–mesenchymal transition (EMT) of tubular epithelial cells (TECs). A unique domain within CTGF (IRTPKISKPIKFELSG) which binds to its potential receptor integrin αvβ3 has been identified. This study was carried out to further characterize a synthetic hexadeca-peptide (P2) homologous to this domain and to determine its effect on CTGF-mediated solid phase cell adhesion, EMT induction and fibrogenesis in rat renal NRK-52E cells. Results showed that both P2 and recombinant CTGF bound to NRK-52E cells. Unlike CTGF, P2 had little effect on EMT induction including cytoskeleton remodeling and expression of α-smooth muscle actin (α-SMA) and E-cadherin, nor did it have effect on fibrogenic induction including alternation of extracellular matrix (ECM) proteins, collagen type I and IV at gene and protein levels. All data showed that P2 bound preferably on the surface of NRK-52E cells and inhibited the effect of CTGF on EMT induction and cell fibrogenesis, probably by occupying the binding sites of CTGF within its potential receptors. Therefore, P2 may be used as a potential anti-fibrotic agent. |
| doi_str_mv | 10.1016/j.cyto.2006.10.009 |
| format | Article |
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The cytokine connective tissue growth factor (CTGF or CCN2) plays an important role in epithelial–mesenchymal transition (EMT) of tubular epithelial cells (TECs). A unique domain within CTGF (IRTPKISKPIKFELSG) which binds to its potential receptor integrin αvβ3 has been identified. This study was carried out to further characterize a synthetic hexadeca-peptide (P2) homologous to this domain and to determine its effect on CTGF-mediated solid phase cell adhesion, EMT induction and fibrogenesis in rat renal NRK-52E cells. Results showed that both P2 and recombinant CTGF bound to NRK-52E cells. Unlike CTGF, P2 had little effect on EMT induction including cytoskeleton remodeling and expression of α-smooth muscle actin (α-SMA) and E-cadherin, nor did it have effect on fibrogenic induction including alternation of extracellular matrix (ECM) proteins, collagen type I and IV at gene and protein levels. All data showed that P2 bound preferably on the surface of NRK-52E cells and inhibited the effect of CTGF on EMT induction and cell fibrogenesis, probably by occupying the binding sites of CTGF within its potential receptors. Therefore, P2 may be used as a potential anti-fibrotic agent.</description><identifier>ISSN: 1043-4666</identifier><identifier>EISSN: 1096-0023</identifier><identifier>DOI: 10.1016/j.cyto.2006.10.009</identifier><identifier>PMID: 17161611</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Actins - genetics ; Actins - metabolism ; Amino Acid Sequence ; Animals ; Cadherins - metabolism ; Cell Differentiation - drug effects ; Cell Line ; Collagen Type I - genetics ; Collagen Type I - metabolism ; Collagen Type IV - genetics ; Collagen Type IV - metabolism ; Connective Tissue Growth Factor ; Cytoskeleton - metabolism ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Epithelial–mesenchymal transition ; Fibrosis ; Humans ; Immediate-Early Proteins - chemistry ; Immediate-Early Proteins - pharmacology ; Integrin alphaVbeta3 - metabolism ; Intercellular Signaling Peptides and Proteins - chemistry ; Intercellular Signaling Peptides and Proteins - pharmacology ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - metabolism ; Molecular Sequence Data ; Muscle, Smooth - metabolism ; Peptide ; Peptide Fragments - chemical synthesis ; Peptide Fragments - chemistry ; Peptide Fragments - pharmacology ; Protein Binding ; Protein Subunits - metabolism ; Rats ; Spectrometry, Mass, Electrospray Ionization ; Transcription, Genetic - genetics ; Tubular epithelial cells</subject><ispartof>Cytokine (Philadelphia, Pa.), 2006-10, Vol.36 (1), p.35-44</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-80f4b6d5a072cbcc2f41bda7fb85d694097ed3286b26e91d77f71366f9f9531b3</citedby><cites>FETCH-LOGICAL-c385t-80f4b6d5a072cbcc2f41bda7fb85d694097ed3286b26e91d77f71366f9f9531b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1043466606002997$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17161611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Yujun</creatorcontrib><creatorcontrib>Tu, Zhidan</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Li, Qing</creatorcontrib><creatorcontrib>Ye, Feng</creatorcontrib><creatorcontrib>Wang, Jinjing</creatorcontrib><creatorcontrib>Qiu, Jing</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Bu, Hong</creatorcontrib><creatorcontrib>Li, Youping</creatorcontrib><title>Homologous peptide of connective tissue growth factor ameliorates epithelial to mesenchymal transition of tubular epithelial cells</title><title>Cytokine (Philadelphia, Pa.)</title><addtitle>Cytokine</addtitle><description>The hallmark of failing renal transplants is tubular atrophy and interstitial fibrosis. The cytokine connective tissue growth factor (CTGF or CCN2) plays an important role in epithelial–mesenchymal transition (EMT) of tubular epithelial cells (TECs). A unique domain within CTGF (IRTPKISKPIKFELSG) which binds to its potential receptor integrin αvβ3 has been identified. This study was carried out to further characterize a synthetic hexadeca-peptide (P2) homologous to this domain and to determine its effect on CTGF-mediated solid phase cell adhesion, EMT induction and fibrogenesis in rat renal NRK-52E cells. Results showed that both P2 and recombinant CTGF bound to NRK-52E cells. Unlike CTGF, P2 had little effect on EMT induction including cytoskeleton remodeling and expression of α-smooth muscle actin (α-SMA) and E-cadherin, nor did it have effect on fibrogenic induction including alternation of extracellular matrix (ECM) proteins, collagen type I and IV at gene and protein levels. All data showed that P2 bound preferably on the surface of NRK-52E cells and inhibited the effect of CTGF on EMT induction and cell fibrogenesis, probably by occupying the binding sites of CTGF within its potential receptors. Therefore, P2 may be used as a potential anti-fibrotic agent.</description><subject>Actins - genetics</subject><subject>Actins - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cadherins - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Collagen Type I - genetics</subject><subject>Collagen Type I - metabolism</subject><subject>Collagen Type IV - genetics</subject><subject>Collagen Type IV - metabolism</subject><subject>Connective Tissue Growth Factor</subject><subject>Cytoskeleton - metabolism</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial–mesenchymal transition</subject><subject>Fibrosis</subject><subject>Humans</subject><subject>Immediate-Early Proteins - chemistry</subject><subject>Immediate-Early Proteins - pharmacology</subject><subject>Integrin alphaVbeta3 - metabolism</subject><subject>Intercellular Signaling Peptides and Proteins - chemistry</subject><subject>Intercellular Signaling Peptides and Proteins - pharmacology</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Smooth - metabolism</subject><subject>Peptide</subject><subject>Peptide Fragments - chemical synthesis</subject><subject>Peptide Fragments - chemistry</subject><subject>Peptide Fragments - pharmacology</subject><subject>Protein Binding</subject><subject>Protein Subunits - metabolism</subject><subject>Rats</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Transcription, Genetic - genetics</subject><subject>Tubular epithelial cells</subject><issn>1043-4666</issn><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-P1CAYh4nRuOvoF_BgOHnryJ8WSuJls1HXZBMveiaUvuwwaUsFumaufnIhM4l70nCA983DL_A-CL2lZE8JFR-Oe3vKYc8IEaWxJ0Q9Q9eUKNEQwvjzem550wohrtCrlI6kEFzKl-iKSirKotfo912YwxQewpbwCmv2I-DgsA3LAjb7R8DZp7QBfojhVz5gZ2wOEZsZJh-iyZAwrD4fSmkmnAOeIcFiD6e5ltEsyWcflpqZt2GbTHzKW5im9Bq9cGZK8Oay79CPz5--394199--fL29uW8s77vc9MS1gxg7QySzg7XMtXQYjXRD341CtURJGDnrxcAEKDpK6STlQjjlVMfpwHfo_Tl3jeHnBinr2af6ArNA-b4WfbncKfVfkFHGFVNtAdkZtDGkFMHpNfrZxJOmRFdF-qirIl0V1V4VsEPvLunbMMP498rFSQE-ngEow3j0EHWyvswURh-LEz0G_6_8P0pUpk8</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Shi, Yujun</creator><creator>Tu, Zhidan</creator><creator>Wang, Wei</creator><creator>Li, Qing</creator><creator>Ye, Feng</creator><creator>Wang, Jinjing</creator><creator>Qiu, Jing</creator><creator>Zhang, Li</creator><creator>Bu, Hong</creator><creator>Li, Youping</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>Homologous peptide of connective tissue growth factor ameliorates epithelial to mesenchymal transition of tubular epithelial cells</title><author>Shi, Yujun ; Tu, Zhidan ; Wang, Wei ; Li, Qing ; Ye, Feng ; Wang, Jinjing ; Qiu, Jing ; Zhang, Li ; Bu, Hong ; Li, Youping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-80f4b6d5a072cbcc2f41bda7fb85d694097ed3286b26e91d77f71366f9f9531b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Actins - genetics</topic><topic>Actins - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Cadherins - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Collagen Type I - genetics</topic><topic>Collagen Type I - metabolism</topic><topic>Collagen Type IV - genetics</topic><topic>Collagen Type IV - metabolism</topic><topic>Connective Tissue Growth Factor</topic><topic>Cytoskeleton - metabolism</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial–mesenchymal transition</topic><topic>Fibrosis</topic><topic>Humans</topic><topic>Immediate-Early Proteins - chemistry</topic><topic>Immediate-Early Proteins - pharmacology</topic><topic>Integrin alphaVbeta3 - metabolism</topic><topic>Intercellular Signaling Peptides and Proteins - chemistry</topic><topic>Intercellular Signaling Peptides and Proteins - pharmacology</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Smooth - metabolism</topic><topic>Peptide</topic><topic>Peptide Fragments - chemical synthesis</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Fragments - pharmacology</topic><topic>Protein Binding</topic><topic>Protein Subunits - metabolism</topic><topic>Rats</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Transcription, Genetic - genetics</topic><topic>Tubular epithelial cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Yujun</creatorcontrib><creatorcontrib>Tu, Zhidan</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Li, Qing</creatorcontrib><creatorcontrib>Ye, Feng</creatorcontrib><creatorcontrib>Wang, Jinjing</creatorcontrib><creatorcontrib>Qiu, Jing</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Bu, Hong</creatorcontrib><creatorcontrib>Li, Youping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cytokine (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Yujun</au><au>Tu, Zhidan</au><au>Wang, Wei</au><au>Li, Qing</au><au>Ye, Feng</au><au>Wang, Jinjing</au><au>Qiu, Jing</au><au>Zhang, Li</au><au>Bu, Hong</au><au>Li, Youping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Homologous peptide of connective tissue growth factor ameliorates epithelial to mesenchymal transition of tubular epithelial cells</atitle><jtitle>Cytokine (Philadelphia, Pa.)</jtitle><addtitle>Cytokine</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>36</volume><issue>1</issue><spage>35</spage><epage>44</epage><pages>35-44</pages><issn>1043-4666</issn><eissn>1096-0023</eissn><abstract>The hallmark of failing renal transplants is tubular atrophy and interstitial fibrosis. The cytokine connective tissue growth factor (CTGF or CCN2) plays an important role in epithelial–mesenchymal transition (EMT) of tubular epithelial cells (TECs). A unique domain within CTGF (IRTPKISKPIKFELSG) which binds to its potential receptor integrin αvβ3 has been identified. This study was carried out to further characterize a synthetic hexadeca-peptide (P2) homologous to this domain and to determine its effect on CTGF-mediated solid phase cell adhesion, EMT induction and fibrogenesis in rat renal NRK-52E cells. Results showed that both P2 and recombinant CTGF bound to NRK-52E cells. Unlike CTGF, P2 had little effect on EMT induction including cytoskeleton remodeling and expression of α-smooth muscle actin (α-SMA) and E-cadherin, nor did it have effect on fibrogenic induction including alternation of extracellular matrix (ECM) proteins, collagen type I and IV at gene and protein levels. All data showed that P2 bound preferably on the surface of NRK-52E cells and inhibited the effect of CTGF on EMT induction and cell fibrogenesis, probably by occupying the binding sites of CTGF within its potential receptors. Therefore, P2 may be used as a potential anti-fibrotic agent.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>17161611</pmid><doi>10.1016/j.cyto.2006.10.009</doi><tpages>10</tpages></addata></record> |
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| subjects | Actins - genetics Actins - metabolism Amino Acid Sequence Animals Cadherins - metabolism Cell Differentiation - drug effects Cell Line Collagen Type I - genetics Collagen Type I - metabolism Collagen Type IV - genetics Collagen Type IV - metabolism Connective Tissue Growth Factor Cytoskeleton - metabolism Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism Epithelial–mesenchymal transition Fibrosis Humans Immediate-Early Proteins - chemistry Immediate-Early Proteins - pharmacology Integrin alphaVbeta3 - metabolism Intercellular Signaling Peptides and Proteins - chemistry Intercellular Signaling Peptides and Proteins - pharmacology Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Molecular Sequence Data Muscle, Smooth - metabolism Peptide Peptide Fragments - chemical synthesis Peptide Fragments - chemistry Peptide Fragments - pharmacology Protein Binding Protein Subunits - metabolism Rats Spectrometry, Mass, Electrospray Ionization Transcription, Genetic - genetics Tubular epithelial cells |
| title | Homologous peptide of connective tissue growth factor ameliorates epithelial to mesenchymal transition of tubular epithelial cells |
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