EcR-A Expression in the Brain and Ovary of the Honeybee (Apis mellifera L.)
We previously demonstrated that six genes involved in ecdysteroid signaling are expressed preferentially in Kenyon-cell subtypes in the mushroom bodies of the honeybee (Apis mellifera L.). To further examine the possible involvement of ecdysteroid signaling in honeybee brain function, we isolated a...
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| Veröffentlicht in: | Zoological Science 2007-06, Vol.24 (6), p.596-603 |
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| creator | Takeuchi, Hideaki Paul, Rajib Kumar Matsuzaka, Emiko Kubo, Takeo |
| description | We previously demonstrated that six genes involved in ecdysteroid signaling are expressed preferentially in Kenyon-cell subtypes in the mushroom bodies of the honeybee (Apis mellifera L.). To further examine the possible involvement of ecdysteroid signaling in honeybee brain function, we isolated a cDNA for the A isoform of the ecdysone receptor gene homolog AmEcR-A and analyzed its expression in the brain. In situ hybridization revealed that AmEcR-A is expressed selectively in the small-type Kenyon cells of the mushroom bodies in the worker and queen brain, like AmE74 and AmHR38, suggesting a possible association of these gene products. Analysis of AmEcR-A expression in queen and worker abdomens demonstrated that AmEcR-A is strongly expressed in nurse cells of the queen ovary, suggesting that ecdysteroid and ecdysteroid signaling have roles in oogenesis. Our present results further support the possible involvement of ecdysteroid signaling in brain function, as well as in regulating queen reproductive physiology in the adult honeybee. |
| doi_str_mv | 10.2108/zsj.24.596 |
| format | Article |
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To further examine the possible involvement of ecdysteroid signaling in honeybee brain function, we isolated a cDNA for the A isoform of the ecdysone receptor gene homolog AmEcR-A and analyzed its expression in the brain. In situ hybridization revealed that AmEcR-A is expressed selectively in the small-type Kenyon cells of the mushroom bodies in the worker and queen brain, like AmE74 and AmHR38, suggesting a possible association of these gene products. Analysis of AmEcR-A expression in queen and worker abdomens demonstrated that AmEcR-A is strongly expressed in nurse cells of the queen ovary, suggesting that ecdysteroid and ecdysteroid signaling have roles in oogenesis. Our present results further support the possible involvement of ecdysteroid signaling in brain function, as well as in regulating queen reproductive physiology in the adult honeybee.</description><identifier>ISSN: 0289-0003</identifier><identifier>DOI: 10.2108/zsj.24.596</identifier><identifier>PMID: 17867861</identifier><language>eng</language><publisher>Japan: Zoological Society of Japan</publisher><subject>Amino Acid Sequence ; Animals ; Apis mellifera ; Bees - genetics ; Bees - metabolism ; Blotting, Northern - veterinary ; Brain - metabolism ; Cloning, Molecular ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; ecdysone receptor ; ecdysteroid ; Female ; gene expression ; Gene Expression Regulation ; honeybee ; In Situ Hybridization - methods ; In Situ Hybridization - veterinary ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Insect Proteins - metabolism ; mushroom body ; Organ Specificity ; ORIGINAL ARTICLES ; ovary ; Ovary - metabolism ; Receptors, Steroid - chemistry ; Receptors, Steroid - genetics ; Receptors, Steroid - metabolism ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; Sequence Alignment - veterinary ; Signal Transduction</subject><ispartof>Zoological Science, 2007-06, Vol.24 (6), p.596-603</ispartof><rights>2007 Zoological Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b472t-99e3d1f8581f02f35b24287620025afec15aba0a00d70077ff3e22247297ef5d3</citedby><cites>FETCH-LOGICAL-b472t-99e3d1f8581f02f35b24287620025afec15aba0a00d70077ff3e22247297ef5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.2108/zsj.24.596$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,776,780,26955,27901,27902,52338</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17867861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takeuchi, Hideaki</creatorcontrib><creatorcontrib>Paul, Rajib Kumar</creatorcontrib><creatorcontrib>Matsuzaka, Emiko</creatorcontrib><creatorcontrib>Kubo, Takeo</creatorcontrib><title>EcR-A Expression in the Brain and Ovary of the Honeybee (Apis mellifera L.)</title><title>Zoological Science</title><addtitle>Zoolog Sci</addtitle><description>We previously demonstrated that six genes involved in ecdysteroid signaling are expressed preferentially in Kenyon-cell subtypes in the mushroom bodies of the honeybee (Apis mellifera L.). To further examine the possible involvement of ecdysteroid signaling in honeybee brain function, we isolated a cDNA for the A isoform of the ecdysone receptor gene homolog AmEcR-A and analyzed its expression in the brain. In situ hybridization revealed that AmEcR-A is expressed selectively in the small-type Kenyon cells of the mushroom bodies in the worker and queen brain, like AmE74 and AmHR38, suggesting a possible association of these gene products. Analysis of AmEcR-A expression in queen and worker abdomens demonstrated that AmEcR-A is strongly expressed in nurse cells of the queen ovary, suggesting that ecdysteroid and ecdysteroid signaling have roles in oogenesis. Our present results further support the possible involvement of ecdysteroid signaling in brain function, as well as in regulating queen reproductive physiology in the adult honeybee.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Apis mellifera</subject><subject>Bees - genetics</subject><subject>Bees - metabolism</subject><subject>Blotting, Northern - veterinary</subject><subject>Brain - metabolism</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>ecdysone receptor</subject><subject>ecdysteroid</subject><subject>Female</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>honeybee</subject><subject>In Situ Hybridization - methods</subject><subject>In Situ Hybridization - veterinary</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>mushroom body</subject><subject>Organ Specificity</subject><subject>ORIGINAL ARTICLES</subject><subject>ovary</subject><subject>Ovary - metabolism</subject><subject>Receptors, Steroid - chemistry</subject><subject>Receptors, Steroid - genetics</subject><subject>Receptors, Steroid - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>Sequence Alignment - veterinary</subject><subject>Signal Transduction</subject><issn>0289-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1LwzAUhnOhuDm98QdIrnQKrSfpR9LLOaYTBwPR65C2J9ixtjPZxPnrzVzRO4VAQvLkPclzCDljEHIG8ubTLUIeh0mWHpA-cJkFABD1yLFzCwAmWcKOSI8JmfrB-uRxUjwFIzr5WFl0rmobWjV0_Yr01mq_0k1J5-_abmlrvrenbYPbHJEOR6vK0RqXy8qg1XQWXp2QQ6OXDk-7eUBe7ibP42kwm98_jEezII8FXwdZhlHJjEwkM8BNlOQ85lKkHIAn2mDBEp1r0AClABDCmAg55_5uJtAkZTQgF_vclW3fNujWqq5c4V-iG2w3TqWSy4hJ-S_IIWY8iYQHr_dgYVvnLBq1slXtv60YqJ1X5b0qHivv1cPnXeomr7H8RTupHrjsgArtz_GuGWrXDJ8DKXRRwz2ZV60X-1fVL-H2i3I</recordid><startdate>200706</startdate><enddate>200706</enddate><creator>Takeuchi, Hideaki</creator><creator>Paul, Rajib Kumar</creator><creator>Matsuzaka, Emiko</creator><creator>Kubo, Takeo</creator><general>Zoological Society of Japan</general><general>UniBio Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200706</creationdate><title>EcR-A Expression in the Brain and Ovary of the Honeybee (Apis mellifera L.)</title><author>Takeuchi, Hideaki ; Paul, Rajib Kumar ; Matsuzaka, Emiko ; Kubo, Takeo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b472t-99e3d1f8581f02f35b24287620025afec15aba0a00d70077ff3e22247297ef5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Apis mellifera</topic><topic>Bees - genetics</topic><topic>Bees - metabolism</topic><topic>Blotting, Northern - veterinary</topic><topic>Brain - metabolism</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>ecdysone receptor</topic><topic>ecdysteroid</topic><topic>Female</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>honeybee</topic><topic>In Situ Hybridization - methods</topic><topic>In Situ Hybridization - veterinary</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>mushroom body</topic><topic>Organ Specificity</topic><topic>ORIGINAL ARTICLES</topic><topic>ovary</topic><topic>Ovary - metabolism</topic><topic>Receptors, Steroid - chemistry</topic><topic>Receptors, Steroid - genetics</topic><topic>Receptors, Steroid - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>Sequence Alignment - veterinary</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takeuchi, Hideaki</creatorcontrib><creatorcontrib>Paul, Rajib Kumar</creatorcontrib><creatorcontrib>Matsuzaka, Emiko</creatorcontrib><creatorcontrib>Kubo, Takeo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Zoological Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takeuchi, Hideaki</au><au>Paul, Rajib Kumar</au><au>Matsuzaka, Emiko</au><au>Kubo, Takeo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EcR-A Expression in the Brain and Ovary of the Honeybee (Apis mellifera L.)</atitle><jtitle>Zoological Science</jtitle><addtitle>Zoolog Sci</addtitle><date>2007-06</date><risdate>2007</risdate><volume>24</volume><issue>6</issue><spage>596</spage><epage>603</epage><pages>596-603</pages><issn>0289-0003</issn><abstract>We previously demonstrated that six genes involved in ecdysteroid signaling are expressed preferentially in Kenyon-cell subtypes in the mushroom bodies of the honeybee (Apis mellifera L.). To further examine the possible involvement of ecdysteroid signaling in honeybee brain function, we isolated a cDNA for the A isoform of the ecdysone receptor gene homolog AmEcR-A and analyzed its expression in the brain. In situ hybridization revealed that AmEcR-A is expressed selectively in the small-type Kenyon cells of the mushroom bodies in the worker and queen brain, like AmE74 and AmHR38, suggesting a possible association of these gene products. Analysis of AmEcR-A expression in queen and worker abdomens demonstrated that AmEcR-A is strongly expressed in nurse cells of the queen ovary, suggesting that ecdysteroid and ecdysteroid signaling have roles in oogenesis. Our present results further support the possible involvement of ecdysteroid signaling in brain function, as well as in regulating queen reproductive physiology in the adult honeybee.</abstract><cop>Japan</cop><pub>Zoological Society of Japan</pub><pmid>17867861</pmid><doi>10.2108/zsj.24.596</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Apis mellifera Bees - genetics Bees - metabolism Blotting, Northern - veterinary Brain - metabolism Cloning, Molecular DNA, Complementary - chemistry DNA, Complementary - genetics ecdysone receptor ecdysteroid Female gene expression Gene Expression Regulation honeybee In Situ Hybridization - methods In Situ Hybridization - veterinary Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - metabolism mushroom body Organ Specificity ORIGINAL ARTICLES ovary Ovary - metabolism Receptors, Steroid - chemistry Receptors, Steroid - genetics Receptors, Steroid - metabolism Reverse Transcriptase Polymerase Chain Reaction - veterinary Sequence Alignment - veterinary Signal Transduction |
| title | EcR-A Expression in the Brain and Ovary of the Honeybee (Apis mellifera L.) |
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