COX-independent Antineoplastic Effects of Sulindac in Oral Cancer are Mediated by Survivin Down-regulation
Background: Cyclooxygenase (COX) inhibitors are reported to exert anti-proliferative and pro-apoptotic effects on cancer. The effects of COX inhibitors on oral squamous cell carcinoma (SCC) and survivin expression were assessed. Materials and Methods: Primary oral SCC were analyzed immunohistochemic...
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| Veröffentlicht in: | Anticancer research 2006-11, Vol.26 (6B), p.4103-4113 |
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| creator | SCHEPER, Mark A SAUK, John J NIKITAKIS, Nikolaos G |
| description | Background: Cyclooxygenase (COX) inhibitors are reported to exert anti-proliferative and pro-apoptotic effects on cancer.
The effects of COX inhibitors on oral squamous cell carcinoma (SCC) and survivin expression were assessed. Materials and Methods:
Primary oral SCC were analyzed immunohistochemically, and oral SCC cell lines were assessed using RT-PCR, Western blot, cell
proliferation and apoptosis assays, following treatment with various COX inhibitors, SiRNA against survivin, or survivin forced
expression. Results: Survivin was expressed in all studied tumors. SiRNA against survivin or treatment with sulindac, but
not other COX inhibitors, decreased survivin expression and tumor cell proliferation and increased apoptosis. Forced expression
of survivin attenuated sulindac's effects. Conclusion: Survivin is implicated as a marker and treatment target in oral SCC,
inhibition of which causes reduction of cell proliferation and induction of apoptosis. Down-regulation of survivin expression
by sulindac provides an explanation for its antineoplastic effects. |
| format | Article |
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The effects of COX inhibitors on oral squamous cell carcinoma (SCC) and survivin expression were assessed. Materials and Methods:
Primary oral SCC were analyzed immunohistochemically, and oral SCC cell lines were assessed using RT-PCR, Western blot, cell
proliferation and apoptosis assays, following treatment with various COX inhibitors, SiRNA against survivin, or survivin forced
expression. Results: Survivin was expressed in all studied tumors. SiRNA against survivin or treatment with sulindac, but
not other COX inhibitors, decreased survivin expression and tumor cell proliferation and increased apoptosis. Forced expression
of survivin attenuated sulindac's effects. Conclusion: Survivin is implicated as a marker and treatment target in oral SCC,
inhibition of which causes reduction of cell proliferation and induction of apoptosis. Down-regulation of survivin expression
by sulindac provides an explanation for its antineoplastic effects.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 17201121</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Apoptosis - drug effects ; Base Sequence ; Biological and medical sciences ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Cell Proliferation - drug effects ; Cyclooxygenase Inhibitors - pharmacology ; DNA Primers ; Down-Regulation - drug effects ; Humans ; Inhibitor of Apoptosis Proteins ; Medical sciences ; Microtubule-Associated Proteins - antagonists & inhibitors ; Microtubule-Associated Proteins - metabolism ; Mouth Neoplasms - metabolism ; Mouth Neoplasms - pathology ; Neoplasm Proteins - antagonists & inhibitors ; Neoplasm Proteins - metabolism ; Oligonucleotide Array Sequence Analysis ; Otorhinolaryngology. Stomatology ; RNA, Small Interfering ; Sulindac - pharmacology ; Tumors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>Anticancer research, 2006-11, Vol.26 (6B), p.4103-4113</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18360806$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17201121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SCHEPER, Mark A</creatorcontrib><creatorcontrib>SAUK, John J</creatorcontrib><creatorcontrib>NIKITAKIS, Nikolaos G</creatorcontrib><title>COX-independent Antineoplastic Effects of Sulindac in Oral Cancer are Mediated by Survivin Down-regulation</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Cyclooxygenase (COX) inhibitors are reported to exert anti-proliferative and pro-apoptotic effects on cancer.
The effects of COX inhibitors on oral squamous cell carcinoma (SCC) and survivin expression were assessed. Materials and Methods:
Primary oral SCC were analyzed immunohistochemically, and oral SCC cell lines were assessed using RT-PCR, Western blot, cell
proliferation and apoptosis assays, following treatment with various COX inhibitors, SiRNA against survivin, or survivin forced
expression. Results: Survivin was expressed in all studied tumors. SiRNA against survivin or treatment with sulindac, but
not other COX inhibitors, decreased survivin expression and tumor cell proliferation and increased apoptosis. Forced expression
of survivin attenuated sulindac's effects. Conclusion: Survivin is implicated as a marker and treatment target in oral SCC,
inhibition of which causes reduction of cell proliferation and induction of apoptosis. Down-regulation of survivin expression
by sulindac provides an explanation for its antineoplastic effects.</description><subject>Apoptosis - drug effects</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Proliferation - drug effects</subject><subject>Cyclooxygenase Inhibitors - pharmacology</subject><subject>DNA Primers</subject><subject>Down-Regulation - drug effects</subject><subject>Humans</subject><subject>Inhibitor of Apoptosis Proteins</subject><subject>Medical sciences</subject><subject>Microtubule-Associated Proteins - antagonists & inhibitors</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Mouth Neoplasms - metabolism</subject><subject>Mouth Neoplasms - pathology</subject><subject>Neoplasm Proteins - antagonists & inhibitors</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>RNA, Small Interfering</subject><subject>Sulindac - pharmacology</subject><subject>Tumors</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0MtOwzAQBVALgWgp_ALyBnaRHDuxk2UpT6moC0BiF02ScevKeWAnrfr3WKKom_Hm6HrunJFprPI4Uqlg52TKeMoixVg6IVfebxmTMs_EJZnEirM45vGUbBer78i0NfYYRjvQeTuYFrvegh9MRZ-0xmrwtNP0Y7QBQkVNS1cOLF1AW6Gj4JC-Y21gwJqWh-DczuwCeuz2beRwPVoYTNdekwsN1uPN8Z2Rr-enz8VrtFy9vC3my2jDFRsiLus6rKYypSXoSoqMaZQ6wzThqUCdoKq4ikUokKsSE52nLC9TyBMOkColZuT-L7d33c-Ifiga4yu0FkKv0Rcy4yrPQsKM3B7hWDZYF70zDbhD8X-dAO6OAHwFVrtQ2PiTy4RkGZOnHzdmvdkbh4VvwNoQKwpwXBbyoUhiJsQvX1l7ZA</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>SCHEPER, Mark A</creator><creator>SAUK, John J</creator><creator>NIKITAKIS, Nikolaos G</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20061101</creationdate><title>COX-independent Antineoplastic Effects of Sulindac in Oral Cancer are Mediated by Survivin Down-regulation</title><author>SCHEPER, Mark A ; SAUK, John J ; NIKITAKIS, Nikolaos G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h270t-26dd121787f6afc6380fe6f8e54253ef4e7c271301197be4f9509b5a942aa5773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Apoptosis - drug effects</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Proliferation - drug effects</topic><topic>Cyclooxygenase Inhibitors - pharmacology</topic><topic>DNA Primers</topic><topic>Down-Regulation - drug effects</topic><topic>Humans</topic><topic>Inhibitor of Apoptosis Proteins</topic><topic>Medical sciences</topic><topic>Microtubule-Associated Proteins - antagonists & inhibitors</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>Mouth Neoplasms - metabolism</topic><topic>Mouth Neoplasms - pathology</topic><topic>Neoplasm Proteins - antagonists & inhibitors</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>RNA, Small Interfering</topic><topic>Sulindac - pharmacology</topic><topic>Tumors</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SCHEPER, Mark A</creatorcontrib><creatorcontrib>SAUK, John J</creatorcontrib><creatorcontrib>NIKITAKIS, Nikolaos G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SCHEPER, Mark A</au><au>SAUK, John J</au><au>NIKITAKIS, Nikolaos G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>COX-independent Antineoplastic Effects of Sulindac in Oral Cancer are Mediated by Survivin Down-regulation</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>26</volume><issue>6B</issue><spage>4103</spage><epage>4113</epage><pages>4103-4113</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: Cyclooxygenase (COX) inhibitors are reported to exert anti-proliferative and pro-apoptotic effects on cancer.
The effects of COX inhibitors on oral squamous cell carcinoma (SCC) and survivin expression were assessed. Materials and Methods:
Primary oral SCC were analyzed immunohistochemically, and oral SCC cell lines were assessed using RT-PCR, Western blot, cell
proliferation and apoptosis assays, following treatment with various COX inhibitors, SiRNA against survivin, or survivin forced
expression. Results: Survivin was expressed in all studied tumors. SiRNA against survivin or treatment with sulindac, but
not other COX inhibitors, decreased survivin expression and tumor cell proliferation and increased apoptosis. Forced expression
of survivin attenuated sulindac's effects. Conclusion: Survivin is implicated as a marker and treatment target in oral SCC,
inhibition of which causes reduction of cell proliferation and induction of apoptosis. Down-regulation of survivin expression
by sulindac provides an explanation for its antineoplastic effects.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>17201121</pmid><tpages>11</tpages></addata></record> |
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| ispartof | Anticancer research, 2006-11, Vol.26 (6B), p.4103-4113 |
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| source | MEDLINE; Free E-Journal (出版社公開部分のみ） |
| subjects | Apoptosis - drug effects Base Sequence Biological and medical sciences Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Cell Proliferation - drug effects Cyclooxygenase Inhibitors - pharmacology DNA Primers Down-Regulation - drug effects Humans Inhibitor of Apoptosis Proteins Medical sciences Microtubule-Associated Proteins - antagonists & inhibitors Microtubule-Associated Proteins - metabolism Mouth Neoplasms - metabolism Mouth Neoplasms - pathology Neoplasm Proteins - antagonists & inhibitors Neoplasm Proteins - metabolism Oligonucleotide Array Sequence Analysis Otorhinolaryngology. Stomatology RNA, Small Interfering Sulindac - pharmacology Tumors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | COX-independent Antineoplastic Effects of Sulindac in Oral Cancer are Mediated by Survivin Down-regulation |
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