Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique
Abstract Vibrio cholerae O1 strains that are hybrids between the classical and El Tor biotypes were isolated during two consecutive years (2004–2005) from diarrheal patients in Mozambique. Similar variants isolated in Bangladesh and recently isolated El Tor strains were analyzed for genetic diversit...
Gespeichert in:
| Veröffentlicht in: | International journal of medical microbiology 2007-10, Vol.297 (6), p.443-449 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 449 |
|---|---|
| container_issue | 6 |
| container_start_page | 443 |
| container_title | International journal of medical microbiology |
| container_volume | 297 |
| creator | Ansaruzzaman, Mohammad Bhuiyan, Nurul A Safa, Ashrafus Sultana, Marzia Mcuamule, Arminda Mondlane, Catarina Wang, Xuan-Yi Deen, Jacqueline L von Seidlein, Lorenz Clemens, John D Lucas, Marcelino Sack, David A Balakrish Nair, Gopinath |
| description | Abstract Vibrio cholerae O1 strains that are hybrids between the classical and El Tor biotypes were isolated during two consecutive years (2004–2005) from diarrheal patients in Mozambique. Similar variants isolated in Bangladesh and recently isolated El Tor strains were analyzed for genetic diversity. Pulsed-field gel electrophoresis (PFGE) analysis using the restriction enzyme NotI , resulted in 18–21 bands showed five closely related PFGE patterns that were distributed similarly in both years (2004–2005) among the 80 strains tested in Mozambique. Overall based on the PFGE patterns the hybrids indicated an El Tor lineage. The restriction patterns of whole-chromosomal DNA grouped the hybrid strains from Mozambique into a separate cluster from Bangladeshi clinical and environmental hybrid strains. A high molecular weight band of 398 kb that contain rstR allele of the classical type was detected from all hybrid strains, which was absent in all conventional classical and El Tor strains. This band could be designated as a marker for the hybrid strains. This study suggests that hybrid strains from Mozambique are closely related to each other, different from Bangladeshi hybrid strains that are diverse in nature and all hybrid strains differed markedly from conventional classical and El Tor strains. |
| doi_str_mv | 10.1016/j.ijmm.2007.01.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68278747</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S1438422107000471</els_id><sourcerecordid>68278747</sourcerecordid><originalsourceid>FETCH-LOGICAL-c440t-b62e30532433bc17c50f95ccc6d797c9e687d8a7dbf0be4bfeb5f00d4faf8d2d3</originalsourceid><addsrcrecordid>eNqFks2L1TAUxYsozof-Ay4kK3etNx9t-kAEHcZRGJmFo9uQJje-1LYZk7yR518_Ke-B4EJXuVx-5wbOOVX1gkJDgXavx8aP89wwANkAbQA2j6pT2tG-hg7k4zIL3teCMXpSnaU0AgDb8O5pdUKlkG3bitMqX-GC2Rti_T3G5POeBEcuJ3IbIkk5ar-kdfPND9EHYrZhwqiR3FDyy-ct2e7L3pIVzIn4FCad0RIXw0ze6-X7pC2mLdGLJZ_Dbz0P_ucOn1VPnJ4SPj--59XXD5e3Fx_r65urTxfvrmsjBOR66BhyaDkTnA-GStOC27TGmM7KjTQb7Hppey3t4GBAMTgcWgdghdOut8zy8-rV4e5dDOXblNXsk8Fp0guGXVJdz2RfrPgvWCymXPa8gOwAmhhSiujUXfSzjntFQa2hqFGtoawKqYCqEkoRvTxe3w0z2j-SYwoFeHMAsJhx7zGqZDwuBq2PaLKywf_7_tu_5Gbyizd6-oF7TGPYxaXYrKhKTIH6stZibQXI0gghKX8AWRC0Jg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20013783</pqid></control><display><type>article</type><title>Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Ansaruzzaman, Mohammad ; Bhuiyan, Nurul A ; Safa, Ashrafus ; Sultana, Marzia ; Mcuamule, Arminda ; Mondlane, Catarina ; Wang, Xuan-Yi ; Deen, Jacqueline L ; von Seidlein, Lorenz ; Clemens, John D ; Lucas, Marcelino ; Sack, David A ; Balakrish Nair, Gopinath</creator><creatorcontrib>Ansaruzzaman, Mohammad ; Bhuiyan, Nurul A ; Safa, Ashrafus ; Sultana, Marzia ; Mcuamule, Arminda ; Mondlane, Catarina ; Wang, Xuan-Yi ; Deen, Jacqueline L ; von Seidlein, Lorenz ; Clemens, John D ; Lucas, Marcelino ; Sack, David A ; Balakrish Nair, Gopinath</creatorcontrib><description>Abstract Vibrio cholerae O1 strains that are hybrids between the classical and El Tor biotypes were isolated during two consecutive years (2004–2005) from diarrheal patients in Mozambique. Similar variants isolated in Bangladesh and recently isolated El Tor strains were analyzed for genetic diversity. Pulsed-field gel electrophoresis (PFGE) analysis using the restriction enzyme NotI , resulted in 18–21 bands showed five closely related PFGE patterns that were distributed similarly in both years (2004–2005) among the 80 strains tested in Mozambique. Overall based on the PFGE patterns the hybrids indicated an El Tor lineage. The restriction patterns of whole-chromosomal DNA grouped the hybrid strains from Mozambique into a separate cluster from Bangladeshi clinical and environmental hybrid strains. A high molecular weight band of 398 kb that contain rstR allele of the classical type was detected from all hybrid strains, which was absent in all conventional classical and El Tor strains. This band could be designated as a marker for the hybrid strains. This study suggests that hybrid strains from Mozambique are closely related to each other, different from Bangladeshi hybrid strains that are diverse in nature and all hybrid strains differed markedly from conventional classical and El Tor strains.</description><identifier>ISSN: 1438-4221</identifier><identifier>EISSN: 1618-0607</identifier><identifier>DOI: 10.1016/j.ijmm.2007.01.009</identifier><identifier>PMID: 17475554</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Bacterial Proteins - genetics ; Bangladesh ; Cholera - epidemiology ; Cholera - microbiology ; Classical ; Cluster Analysis ; Deoxyribonucleases, Type II Site-Specific - metabolism ; DNA ; DNA Fingerprinting ; DNA, Bacterial - genetics ; El Tor ; Electrophoresis, Gel, Pulsed-Field ; Genetic Markers ; Genotype ; Humans ; Hybrid ; Infectious Disease ; Medical Education ; Molecular Epidemiology ; Mozambique ; PCR ; PFGE ; Polymorphism, Genetic ; Repressor Proteins - genetics ; V. cholerae O1 ; Vibrio cholerae ; Vibrio cholerae O1 - genetics ; Vibrio cholerae O1 - isolation & purification</subject><ispartof>International journal of medical microbiology, 2007-10, Vol.297 (6), p.443-449</ispartof><rights>Elsevier GmbH</rights><rights>2007 Elsevier GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-b62e30532433bc17c50f95ccc6d797c9e687d8a7dbf0be4bfeb5f00d4faf8d2d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijmm.2007.01.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17475554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ansaruzzaman, Mohammad</creatorcontrib><creatorcontrib>Bhuiyan, Nurul A</creatorcontrib><creatorcontrib>Safa, Ashrafus</creatorcontrib><creatorcontrib>Sultana, Marzia</creatorcontrib><creatorcontrib>Mcuamule, Arminda</creatorcontrib><creatorcontrib>Mondlane, Catarina</creatorcontrib><creatorcontrib>Wang, Xuan-Yi</creatorcontrib><creatorcontrib>Deen, Jacqueline L</creatorcontrib><creatorcontrib>von Seidlein, Lorenz</creatorcontrib><creatorcontrib>Clemens, John D</creatorcontrib><creatorcontrib>Lucas, Marcelino</creatorcontrib><creatorcontrib>Sack, David A</creatorcontrib><creatorcontrib>Balakrish Nair, Gopinath</creatorcontrib><title>Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique</title><title>International journal of medical microbiology</title><addtitle>Int J Med Microbiol</addtitle><description>Abstract Vibrio cholerae O1 strains that are hybrids between the classical and El Tor biotypes were isolated during two consecutive years (2004–2005) from diarrheal patients in Mozambique. Similar variants isolated in Bangladesh and recently isolated El Tor strains were analyzed for genetic diversity. Pulsed-field gel electrophoresis (PFGE) analysis using the restriction enzyme NotI , resulted in 18–21 bands showed five closely related PFGE patterns that were distributed similarly in both years (2004–2005) among the 80 strains tested in Mozambique. Overall based on the PFGE patterns the hybrids indicated an El Tor lineage. The restriction patterns of whole-chromosomal DNA grouped the hybrid strains from Mozambique into a separate cluster from Bangladeshi clinical and environmental hybrid strains. A high molecular weight band of 398 kb that contain rstR allele of the classical type was detected from all hybrid strains, which was absent in all conventional classical and El Tor strains. This band could be designated as a marker for the hybrid strains. This study suggests that hybrid strains from Mozambique are closely related to each other, different from Bangladeshi hybrid strains that are diverse in nature and all hybrid strains differed markedly from conventional classical and El Tor strains.</description><subject>Bacterial Proteins - genetics</subject><subject>Bangladesh</subject><subject>Cholera - epidemiology</subject><subject>Cholera - microbiology</subject><subject>Classical</subject><subject>Cluster Analysis</subject><subject>Deoxyribonucleases, Type II Site-Specific - metabolism</subject><subject>DNA</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - genetics</subject><subject>El Tor</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Genetic Markers</subject><subject>Genotype</subject><subject>Humans</subject><subject>Hybrid</subject><subject>Infectious Disease</subject><subject>Medical Education</subject><subject>Molecular Epidemiology</subject><subject>Mozambique</subject><subject>PCR</subject><subject>PFGE</subject><subject>Polymorphism, Genetic</subject><subject>Repressor Proteins - genetics</subject><subject>V. cholerae O1</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae O1 - genetics</subject><subject>Vibrio cholerae O1 - isolation & purification</subject><issn>1438-4221</issn><issn>1618-0607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2L1TAUxYsozof-Ay4kK3etNx9t-kAEHcZRGJmFo9uQJje-1LYZk7yR518_Ke-B4EJXuVx-5wbOOVX1gkJDgXavx8aP89wwANkAbQA2j6pT2tG-hg7k4zIL3teCMXpSnaU0AgDb8O5pdUKlkG3bitMqX-GC2Rti_T3G5POeBEcuJ3IbIkk5ar-kdfPND9EHYrZhwqiR3FDyy-ct2e7L3pIVzIn4FCad0RIXw0ze6-X7pC2mLdGLJZ_Dbz0P_ucOn1VPnJ4SPj--59XXD5e3Fx_r65urTxfvrmsjBOR66BhyaDkTnA-GStOC27TGmM7KjTQb7Hppey3t4GBAMTgcWgdghdOut8zy8-rV4e5dDOXblNXsk8Fp0guGXVJdz2RfrPgvWCymXPa8gOwAmhhSiujUXfSzjntFQa2hqFGtoawKqYCqEkoRvTxe3w0z2j-SYwoFeHMAsJhx7zGqZDwuBq2PaLKywf_7_tu_5Gbyizd6-oF7TGPYxaXYrKhKTIH6stZibQXI0gghKX8AWRC0Jg</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Ansaruzzaman, Mohammad</creator><creator>Bhuiyan, Nurul A</creator><creator>Safa, Ashrafus</creator><creator>Sultana, Marzia</creator><creator>Mcuamule, Arminda</creator><creator>Mondlane, Catarina</creator><creator>Wang, Xuan-Yi</creator><creator>Deen, Jacqueline L</creator><creator>von Seidlein, Lorenz</creator><creator>Clemens, John D</creator><creator>Lucas, Marcelino</creator><creator>Sack, David A</creator><creator>Balakrish Nair, Gopinath</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique</title><author>Ansaruzzaman, Mohammad ; Bhuiyan, Nurul A ; Safa, Ashrafus ; Sultana, Marzia ; Mcuamule, Arminda ; Mondlane, Catarina ; Wang, Xuan-Yi ; Deen, Jacqueline L ; von Seidlein, Lorenz ; Clemens, John D ; Lucas, Marcelino ; Sack, David A ; Balakrish Nair, Gopinath</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-b62e30532433bc17c50f95ccc6d797c9e687d8a7dbf0be4bfeb5f00d4faf8d2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bangladesh</topic><topic>Cholera - epidemiology</topic><topic>Cholera - microbiology</topic><topic>Classical</topic><topic>Cluster Analysis</topic><topic>Deoxyribonucleases, Type II Site-Specific - metabolism</topic><topic>DNA</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - genetics</topic><topic>El Tor</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Genetic Markers</topic><topic>Genotype</topic><topic>Humans</topic><topic>Hybrid</topic><topic>Infectious Disease</topic><topic>Medical Education</topic><topic>Molecular Epidemiology</topic><topic>Mozambique</topic><topic>PCR</topic><topic>PFGE</topic><topic>Polymorphism, Genetic</topic><topic>Repressor Proteins - genetics</topic><topic>V. cholerae O1</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae O1 - genetics</topic><topic>Vibrio cholerae O1 - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ansaruzzaman, Mohammad</creatorcontrib><creatorcontrib>Bhuiyan, Nurul A</creatorcontrib><creatorcontrib>Safa, Ashrafus</creatorcontrib><creatorcontrib>Sultana, Marzia</creatorcontrib><creatorcontrib>Mcuamule, Arminda</creatorcontrib><creatorcontrib>Mondlane, Catarina</creatorcontrib><creatorcontrib>Wang, Xuan-Yi</creatorcontrib><creatorcontrib>Deen, Jacqueline L</creatorcontrib><creatorcontrib>von Seidlein, Lorenz</creatorcontrib><creatorcontrib>Clemens, John D</creatorcontrib><creatorcontrib>Lucas, Marcelino</creatorcontrib><creatorcontrib>Sack, David A</creatorcontrib><creatorcontrib>Balakrish Nair, Gopinath</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ansaruzzaman, Mohammad</au><au>Bhuiyan, Nurul A</au><au>Safa, Ashrafus</au><au>Sultana, Marzia</au><au>Mcuamule, Arminda</au><au>Mondlane, Catarina</au><au>Wang, Xuan-Yi</au><au>Deen, Jacqueline L</au><au>von Seidlein, Lorenz</au><au>Clemens, John D</au><au>Lucas, Marcelino</au><au>Sack, David A</au><au>Balakrish Nair, Gopinath</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique</atitle><jtitle>International journal of medical microbiology</jtitle><addtitle>Int J Med Microbiol</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>297</volume><issue>6</issue><spage>443</spage><epage>449</epage><pages>443-449</pages><issn>1438-4221</issn><eissn>1618-0607</eissn><abstract>Abstract Vibrio cholerae O1 strains that are hybrids between the classical and El Tor biotypes were isolated during two consecutive years (2004–2005) from diarrheal patients in Mozambique. Similar variants isolated in Bangladesh and recently isolated El Tor strains were analyzed for genetic diversity. Pulsed-field gel electrophoresis (PFGE) analysis using the restriction enzyme NotI , resulted in 18–21 bands showed five closely related PFGE patterns that were distributed similarly in both years (2004–2005) among the 80 strains tested in Mozambique. Overall based on the PFGE patterns the hybrids indicated an El Tor lineage. The restriction patterns of whole-chromosomal DNA grouped the hybrid strains from Mozambique into a separate cluster from Bangladeshi clinical and environmental hybrid strains. A high molecular weight band of 398 kb that contain rstR allele of the classical type was detected from all hybrid strains, which was absent in all conventional classical and El Tor strains. This band could be designated as a marker for the hybrid strains. This study suggests that hybrid strains from Mozambique are closely related to each other, different from Bangladeshi hybrid strains that are diverse in nature and all hybrid strains differed markedly from conventional classical and El Tor strains.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>17475554</pmid><doi>10.1016/j.ijmm.2007.01.009</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1438-4221 |
| ispartof | International journal of medical microbiology, 2007-10, Vol.297 (6), p.443-449 |
| issn | 1438-4221 1618-0607 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68278747 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacterial Proteins - genetics Bangladesh Cholera - epidemiology Cholera - microbiology Classical Cluster Analysis Deoxyribonucleases, Type II Site-Specific - metabolism DNA DNA Fingerprinting DNA, Bacterial - genetics El Tor Electrophoresis, Gel, Pulsed-Field Genetic Markers Genotype Humans Hybrid Infectious Disease Medical Education Molecular Epidemiology Mozambique PCR PFGE Polymorphism, Genetic Repressor Proteins - genetics V. cholerae O1 Vibrio cholerae Vibrio cholerae O1 - genetics Vibrio cholerae O1 - isolation & purification |
| title | Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T18%3A29%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20diversity%20of%20El%20Tor%20strains%20of%20Vibrio%20cholerae%20O1%20with%20hybrid%20traits%20isolated%20from%20Bangladesh%20and%20Mozambique&rft.jtitle=International%20journal%20of%20medical%20microbiology&rft.au=Ansaruzzaman,%20Mohammad&rft.date=2007-10-01&rft.volume=297&rft.issue=6&rft.spage=443&rft.epage=449&rft.pages=443-449&rft.issn=1438-4221&rft.eissn=1618-0607&rft_id=info:doi/10.1016/j.ijmm.2007.01.009&rft_dat=%3Cproquest_cross%3E68278747%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20013783&rft_id=info:pmid/17475554&rft_els_id=1_s2_0_S1438422107000471&rfr_iscdi=true |