Contribution of SPI-4 genes to the virulence of Salmonella enterica
Abstract Salmonella pathogenicity island-4 (SPI-4) is a 27-kb region that carries six genes designated siiABCDEF. SiiC, SiiD, and SiiF form a type I secretion apparatus for the secretion of SiiE, a huge (∼600 kDa) protein contributing to the colonization of the bovine intestines. Here it is shown th...
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| Veröffentlicht in: | FEMS microbiology letters 2007-10, Vol.275 (1), p.153-159 |
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| creator | Kiss, Tünde Morgan, Eirwen Nagy, Gábor |
| description | Abstract
Salmonella pathogenicity island-4 (SPI-4) is a 27-kb region that carries six genes designated siiABCDEF. SiiC, SiiD, and SiiF form a type I secretion apparatus for the secretion of SiiE, a huge (∼600 kDa) protein contributing to the colonization of the bovine intestines. Here it is shown that loss of SPI-4 attenuates the oral virulence of Salmonella enterica serovars Typhimurium and Enteritidis in mice. Fifty percent lethal doses were elevated in both serovars upon the loss of SPI-4. Moreover, ΔSPI-4 mutants were outcompeted in systemic organs by their wild-type strains in a cochallenge model. Contribution of SPI-4 to virulence appeared less pronounced in the S. Enteritidis strain, which was justified by lower levels of the secreted protein SiiE in this strain in comparison with S. Typhimurium. Competition assays with isogenic mutants lacking individual genes of the island showed that all six genes were required for full virulence of S. Typhimurium. ΔsiiA and ΔsiiB mutants were, nevertheless, able to secrete SiiE to culture supernatants. The amount of secreted SiiE was, however, reduced in these two mutants compared with the wild-type strain. Furthermore, a down-regulation of SiiE levels is shown in structural and regulatory lipopolysaccharide mutants exhibiting the deep-rough phenotype. |
| doi_str_mv | 10.1111/j.1574-6968.2007.00871.x |
| format | Article |
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Salmonella pathogenicity island-4 (SPI-4) is a 27-kb region that carries six genes designated siiABCDEF. SiiC, SiiD, and SiiF form a type I secretion apparatus for the secretion of SiiE, a huge (∼600 kDa) protein contributing to the colonization of the bovine intestines. Here it is shown that loss of SPI-4 attenuates the oral virulence of Salmonella enterica serovars Typhimurium and Enteritidis in mice. Fifty percent lethal doses were elevated in both serovars upon the loss of SPI-4. Moreover, ΔSPI-4 mutants were outcompeted in systemic organs by their wild-type strains in a cochallenge model. Contribution of SPI-4 to virulence appeared less pronounced in the S. Enteritidis strain, which was justified by lower levels of the secreted protein SiiE in this strain in comparison with S. Typhimurium. Competition assays with isogenic mutants lacking individual genes of the island showed that all six genes were required for full virulence of S. Typhimurium. ΔsiiA and ΔsiiB mutants were, nevertheless, able to secrete SiiE to culture supernatants. The amount of secreted SiiE was, however, reduced in these two mutants compared with the wild-type strain. Furthermore, a down-regulation of SiiE levels is shown in structural and regulatory lipopolysaccharide mutants exhibiting the deep-rough phenotype.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2007.00871.x</identifier><identifier>PMID: 17711458</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Colonization ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Genes ; Genes, Bacterial - genetics ; Genomic Islands - genetics ; Intestine ; Levels ; lipopolysaccharide ; Lipopolysaccharides ; Lipopolysaccharides - chemistry ; Lipopolysaccharides - metabolism ; Mice ; Mice, Inbred BALB C ; Microbiology ; Mouth - microbiology ; Mutants ; Organs ; Pathogenicity ; Pathogens ; Phenotypes ; Proteins ; Salmonella ; Salmonella enterica ; Salmonella enteritidis - genetics ; Salmonella enteritidis - pathogenicity ; Salmonella Infections - microbiology ; Salmonella pathogenicity island‐4 ; Salmonella typhimurium - genetics ; Salmonella typhimurium - pathogenicity ; Secretion ; sii genes ; type I secretion ; Virulence ; Virulence - genetics</subject><ispartof>FEMS microbiology letters, 2007-10, Vol.275 (1), p.153-159</ispartof><rights>2007 Federation of European Microbiological Societies 2007</rights><rights>2008 INIST-CNRS</rights><rights>2007 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4681-bfb4b7fc9eae54ae10d1e3595756680fe6eb6cffa4efe3ae31c1ef6e1d02e70a3</citedby><cites>FETCH-LOGICAL-c4681-bfb4b7fc9eae54ae10d1e3595756680fe6eb6cffa4efe3ae31c1ef6e1d02e70a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2007.00871.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2007.00871.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19033294$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17711458$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kiss, Tünde</creatorcontrib><creatorcontrib>Morgan, Eirwen</creatorcontrib><creatorcontrib>Nagy, Gábor</creatorcontrib><title>Contribution of SPI-4 genes to the virulence of Salmonella enterica</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Salmonella pathogenicity island-4 (SPI-4) is a 27-kb region that carries six genes designated siiABCDEF. SiiC, SiiD, and SiiF form a type I secretion apparatus for the secretion of SiiE, a huge (∼600 kDa) protein contributing to the colonization of the bovine intestines. Here it is shown that loss of SPI-4 attenuates the oral virulence of Salmonella enterica serovars Typhimurium and Enteritidis in mice. Fifty percent lethal doses were elevated in both serovars upon the loss of SPI-4. Moreover, ΔSPI-4 mutants were outcompeted in systemic organs by their wild-type strains in a cochallenge model. Contribution of SPI-4 to virulence appeared less pronounced in the S. Enteritidis strain, which was justified by lower levels of the secreted protein SiiE in this strain in comparison with S. Typhimurium. Competition assays with isogenic mutants lacking individual genes of the island showed that all six genes were required for full virulence of S. Typhimurium. ΔsiiA and ΔsiiB mutants were, nevertheless, able to secrete SiiE to culture supernatants. The amount of secreted SiiE was, however, reduced in these two mutants compared with the wild-type strain. Furthermore, a down-regulation of SiiE levels is shown in structural and regulatory lipopolysaccharide mutants exhibiting the deep-rough phenotype.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Colonization</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genes, Bacterial - genetics</subject><subject>Genomic Islands - genetics</subject><subject>Intestine</subject><subject>Levels</subject><subject>lipopolysaccharide</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - chemistry</subject><subject>Lipopolysaccharides - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Mouth - microbiology</subject><subject>Mutants</subject><subject>Organs</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Phenotypes</subject><subject>Proteins</subject><subject>Salmonella</subject><subject>Salmonella enterica</subject><subject>Salmonella enteritidis - genetics</subject><subject>Salmonella enteritidis - pathogenicity</subject><subject>Salmonella Infections - microbiology</subject><subject>Salmonella pathogenicity island‐4</subject><subject>Salmonella typhimurium - genetics</subject><subject>Salmonella typhimurium - pathogenicity</subject><subject>Secretion</subject><subject>sii genes</subject><subject>type I secretion</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkF1rFDEUhoModtv6F8qA6N2MJx-TzIA3stgPWFGwXodM9qTOMpusyYy2_76Z7tKCRTA3CZznzXl5CCkoVDSfD5uK1kqUspVNxQBUBdAoWt2-IIvHwUuyAK6akkKrjshxShsAEAzka3JElaJU1M2CLJfBj7HvprEPvgiu-P7tqhTFDXpMxRiK8ScWv_s4DegtPszNsA0eh8EU6EeMvTWn5JUzQ8I3h_uE_Dj_fL28LFdfL66Wn1alFbKhZec60SlnWzRYC4MU1hR53daqlrIBhxI7aZ0zAh1yg5xaik4iXQNDBYafkPf7f3cx_JowjXrbJztX8RimpGXDFGeKZfDtX-AmTNHnbppxkDWTolWZavaUjSGliE7vYr818U5T0LNmvdGzTT3b1LNm_aBZ3-bo2WHB1G1x_RQ8eM3AuwNgkjWDi8bbPj1xLXDOWpG5j3vuTz_g3X8X0OdfVvmR43wfD9PuH-Hyeft7qQ-mvg</recordid><startdate>200710</startdate><enddate>200710</enddate><creator>Kiss, Tünde</creator><creator>Morgan, Eirwen</creator><creator>Nagy, Gábor</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200710</creationdate><title>Contribution of SPI-4 genes to the virulence of Salmonella enterica</title><author>Kiss, Tünde ; Morgan, Eirwen ; Nagy, Gábor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4681-bfb4b7fc9eae54ae10d1e3595756680fe6eb6cffa4efe3ae31c1ef6e1d02e70a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Colonization</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genes, Bacterial - genetics</topic><topic>Genomic Islands - genetics</topic><topic>Intestine</topic><topic>Levels</topic><topic>lipopolysaccharide</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - chemistry</topic><topic>Lipopolysaccharides - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Mouth - microbiology</topic><topic>Mutants</topic><topic>Organs</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Phenotypes</topic><topic>Proteins</topic><topic>Salmonella</topic><topic>Salmonella enterica</topic><topic>Salmonella enteritidis - genetics</topic><topic>Salmonella enteritidis - pathogenicity</topic><topic>Salmonella Infections - microbiology</topic><topic>Salmonella pathogenicity island‐4</topic><topic>Salmonella typhimurium - genetics</topic><topic>Salmonella typhimurium - pathogenicity</topic><topic>Secretion</topic><topic>sii genes</topic><topic>type I secretion</topic><topic>Virulence</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiss, Tünde</creatorcontrib><creatorcontrib>Morgan, Eirwen</creatorcontrib><creatorcontrib>Nagy, Gábor</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiss, Tünde</au><au>Morgan, Eirwen</au><au>Nagy, Gábor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contribution of SPI-4 genes to the virulence of Salmonella enterica</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2007-10</date><risdate>2007</risdate><volume>275</volume><issue>1</issue><spage>153</spage><epage>159</epage><pages>153-159</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Salmonella pathogenicity island-4 (SPI-4) is a 27-kb region that carries six genes designated siiABCDEF. SiiC, SiiD, and SiiF form a type I secretion apparatus for the secretion of SiiE, a huge (∼600 kDa) protein contributing to the colonization of the bovine intestines. Here it is shown that loss of SPI-4 attenuates the oral virulence of Salmonella enterica serovars Typhimurium and Enteritidis in mice. Fifty percent lethal doses were elevated in both serovars upon the loss of SPI-4. Moreover, ΔSPI-4 mutants were outcompeted in systemic organs by their wild-type strains in a cochallenge model. Contribution of SPI-4 to virulence appeared less pronounced in the S. Enteritidis strain, which was justified by lower levels of the secreted protein SiiE in this strain in comparison with S. Typhimurium. Competition assays with isogenic mutants lacking individual genes of the island showed that all six genes were required for full virulence of S. Typhimurium. ΔsiiA and ΔsiiB mutants were, nevertheless, able to secrete SiiE to culture supernatants. The amount of secreted SiiE was, however, reduced in these two mutants compared with the wild-type strain. Furthermore, a down-regulation of SiiE levels is shown in structural and regulatory lipopolysaccharide mutants exhibiting the deep-rough phenotype.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17711458</pmid><doi>10.1111/j.1574-6968.2007.00871.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Colonization Female Fundamental and applied biological sciences. Psychology Gene Deletion Gene Expression Regulation, Bacterial Genes Genes, Bacterial - genetics Genomic Islands - genetics Intestine Levels lipopolysaccharide Lipopolysaccharides Lipopolysaccharides - chemistry Lipopolysaccharides - metabolism Mice Mice, Inbred BALB C Microbiology Mouth - microbiology Mutants Organs Pathogenicity Pathogens Phenotypes Proteins Salmonella Salmonella enterica Salmonella enteritidis - genetics Salmonella enteritidis - pathogenicity Salmonella Infections - microbiology Salmonella pathogenicity island‐4 Salmonella typhimurium - genetics Salmonella typhimurium - pathogenicity Secretion sii genes type I secretion Virulence Virulence - genetics |
| title | Contribution of SPI-4 genes to the virulence of Salmonella enterica |
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