Spatial and Temporal Control of Microwave Triggered Chemiluminescence: A Protein Detection Platform
We have combined the principles of microwave circuitry and antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now “trigger” chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. With this technology platform, we achie...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2007-09, Vol.79 (18), p.7042-7052 |
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| creator | Previte, Michael J. R Aslan, Kadir Geddes, Chris D |
| description | We have combined the principles of microwave circuitry and antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now “trigger” chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. With this technology platform, we achieve spatial and temporal control of enzyme and chemically catalyzed chemiluminescence reactions to achieve more than 500-fold increases in “on-demand” photon flux from chemically catalyzed chemiluminescent reactions. We also report a 6-fold increase in photon flux from HRP-catalyzed assays on disposable coverslips functionalized with HRP and placed proximal to the substrates modified with thin-film aluminum triangle disjointed “bow-tie” structures. In addition, we demonstrate the applicability of this technology to develop multiplexed or high-throughput chemiluminescent assays. We also demonstrate the clinical and biological relevance of this technology platform by affixing aluminum structures in proximity to HRP protein immobilized on nitrocellulose to improve the sensitivity for this model Western blot scheme by 50-fold. We believe analytical applications that rely on enzyme-catalyzed chemiluminescence, such as immunoassays, may greatly benefit from this new platform technology. |
| doi_str_mv | 10.1021/ac071042+ |
| format | Article |
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R ; Aslan, Kadir ; Geddes, Chris D</creator><creatorcontrib>Previte, Michael J. R ; Aslan, Kadir ; Geddes, Chris D</creatorcontrib><description>We have combined the principles of microwave circuitry and antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now “trigger” chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. With this technology platform, we achieve spatial and temporal control of enzyme and chemically catalyzed chemiluminescence reactions to achieve more than 500-fold increases in “on-demand” photon flux from chemically catalyzed chemiluminescent reactions. We also report a 6-fold increase in photon flux from HRP-catalyzed assays on disposable coverslips functionalized with HRP and placed proximal to the substrates modified with thin-film aluminum triangle disjointed “bow-tie” structures. In addition, we demonstrate the applicability of this technology to develop multiplexed or high-throughput chemiluminescent assays. We also demonstrate the clinical and biological relevance of this technology platform by affixing aluminum structures in proximity to HRP protein immobilized on nitrocellulose to improve the sensitivity for this model Western blot scheme by 50-fold. 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R</creatorcontrib><creatorcontrib>Aslan, Kadir</creatorcontrib><creatorcontrib>Geddes, Chris D</creatorcontrib><title>Spatial and Temporal Control of Microwave Triggered Chemiluminescence: A Protein Detection Platform</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>We have combined the principles of microwave circuitry and antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now “trigger” chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. With this technology platform, we achieve spatial and temporal control of enzyme and chemically catalyzed chemiluminescence reactions to achieve more than 500-fold increases in “on-demand” photon flux from chemically catalyzed chemiluminescent reactions. We also report a 6-fold increase in photon flux from HRP-catalyzed assays on disposable coverslips functionalized with HRP and placed proximal to the substrates modified with thin-film aluminum triangle disjointed “bow-tie” structures. In addition, we demonstrate the applicability of this technology to develop multiplexed or high-throughput chemiluminescent assays. We also demonstrate the clinical and biological relevance of this technology platform by affixing aluminum structures in proximity to HRP protein immobilized on nitrocellulose to improve the sensitivity for this model Western blot scheme by 50-fold. We believe analytical applications that rely on enzyme-catalyzed chemiluminescence, such as immunoassays, may greatly benefit from this new platform technology.</description><subject>Aluminum - chemistry</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>Catalysis</subject><subject>Chemical and thermal methods</subject><subject>Chemical reactions</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Glass - chemistry</subject><subject>Horseradish Peroxidase - chemistry</subject><subject>Kinetics</subject><subject>Luminescence</subject><subject>Luminescent Measurements - economics</subject><subject>Luminescent Measurements - instrumentation</subject><subject>Luminescent Measurements - methods</subject><subject>Microwaves</subject><subject>Miscellaneous</subject><subject>Proteins</subject><subject>Proteins - analysis</subject><subject>Proteins - chemistry</subject><subject>Sensitivity and Specificity</subject><subject>Surface Properties</subject><subject>Temperature</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0N1qFDEUB_Agit1WL3wBCaIiyGhOZjaZeFe29YsWF7p6GzKZk5o6M1mTmdreeetr9klM2dUFhZBDyI_DOX9CHgF7BYzDa2OZBFbxl3fIDOacFaKu-V0yY4yVBZeM7ZH9lC4YA2Ag7pM9kEKJSskZcWdrM3rTUTO0dIX9OsT8WIRhjKGjwdFTb2P4YS6RrqI_P8eILV18xd53U-8HTBYHi29ufv6ih3QZw4h-oEc4oh19GOiyM6MLsX9A7jnTJXy4rQfk89vj1eJ9cfLp3YfF4UlhKgljvq2qK1k6Ll0jLQonoGyss1a12FrDSweNaGWuc-UcKGhQAZNla6vGNk15QJ5v-q5j-D5hGnXv84hdZwYMU9Ki5pIDVBk--QdehCkOeTbNQdZSzUFl9GKDcgQpRXR6HX1v4rUGpm-T13-Sz_Txtt_U9Nju4DbpDJ5tgUnWdC6awfq0c4rVPJ_sio3zacSrv_8mftNClnKuV8szfXS65F9E_VHf-qcbb2za7fDffL8B93emuQ</recordid><startdate>20070915</startdate><enddate>20070915</enddate><creator>Previte, Michael J. 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R ; Aslan, Kadir ; Geddes, Chris D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a471t-a4c98473f27fb7ce6f613bcfcc9dedca23f1b6d723f59ff191be91073dc4bcbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aluminum - chemistry</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Blotting, Western</topic><topic>Catalysis</topic><topic>Chemical and thermal methods</topic><topic>Chemical reactions</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Glass - chemistry</topic><topic>Horseradish Peroxidase - chemistry</topic><topic>Kinetics</topic><topic>Luminescence</topic><topic>Luminescent Measurements - economics</topic><topic>Luminescent Measurements - instrumentation</topic><topic>Luminescent Measurements - methods</topic><topic>Microwaves</topic><topic>Miscellaneous</topic><topic>Proteins</topic><topic>Proteins - analysis</topic><topic>Proteins - chemistry</topic><topic>Sensitivity and Specificity</topic><topic>Surface Properties</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Previte, Michael J. R</creatorcontrib><creatorcontrib>Aslan, Kadir</creatorcontrib><creatorcontrib>Geddes, Chris D</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Previte, Michael J. R</au><au>Aslan, Kadir</au><au>Geddes, Chris D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatial and Temporal Control of Microwave Triggered Chemiluminescence: A Protein Detection Platform</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2007-09-15</date><risdate>2007</risdate><volume>79</volume><issue>18</issue><spage>7042</spage><epage>7052</epage><pages>7042-7052</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>We have combined the principles of microwave circuitry and antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now “trigger” chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. With this technology platform, we achieve spatial and temporal control of enzyme and chemically catalyzed chemiluminescence reactions to achieve more than 500-fold increases in “on-demand” photon flux from chemically catalyzed chemiluminescent reactions. We also report a 6-fold increase in photon flux from HRP-catalyzed assays on disposable coverslips functionalized with HRP and placed proximal to the substrates modified with thin-film aluminum triangle disjointed “bow-tie” structures. In addition, we demonstrate the applicability of this technology to develop multiplexed or high-throughput chemiluminescent assays. We also demonstrate the clinical and biological relevance of this technology platform by affixing aluminum structures in proximity to HRP protein immobilized on nitrocellulose to improve the sensitivity for this model Western blot scheme by 50-fold. We believe analytical applications that rely on enzyme-catalyzed chemiluminescence, such as immunoassays, may greatly benefit from this new platform technology.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17696497</pmid><doi>10.1021/ac071042+</doi><tpages>11</tpages></addata></record> |
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| subjects | Aluminum - chemistry Analytical chemistry Animals Blotting, Western Catalysis Chemical and thermal methods Chemical reactions Chemistry Exact sciences and technology Glass - chemistry Horseradish Peroxidase - chemistry Kinetics Luminescence Luminescent Measurements - economics Luminescent Measurements - instrumentation Luminescent Measurements - methods Microwaves Miscellaneous Proteins Proteins - analysis Proteins - chemistry Sensitivity and Specificity Surface Properties Temperature |
| title | Spatial and Temporal Control of Microwave Triggered Chemiluminescence: A Protein Detection Platform |
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