cDNA cloning and tissue expression of plasma lysozyme in the eastern oyster, Crassostrea virginica
The cDNA sequence of a 17,861 Da lysozyme first purified from plasma of eastern oysters ( Crassostrea virginica) was identified and its complete amino acid sequence deduced. The amino acid sequence of the plasma lysozyme, designated cv-lysozyme 1, contained both a unique and a conserved region when...
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| Veröffentlicht in: | Fish & Shellfish Immunology 2007-11, Vol.23 (5), p.957-968 |
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| creator | Itoh, Naoki Xue, QingGang Li, Yanli Cooper, Richard K. La Peyre, Jerome F. |
| description | The cDNA sequence of a 17,861
Da lysozyme first purified from plasma of eastern oysters (
Crassostrea virginica) was identified and its complete amino acid sequence deduced. The amino acid sequence of the plasma lysozyme, designated cv-lysozyme 1, contained both a unique and a conserved region when compared to the amino acid sequences of other bivalve lysozymes.
In situ hybridisation located cv-lysozyme 1 gene expression in mantle and gill cells in standard histological sections. Quantitative real-time RT-PCR detected cv-lysozyme 1 expression in all organs examined and circulating haemocytes. The number of cv-lysozyme 1 mRNA transcripts was particularly high in mantles and labial palps suggesting those organs are the main sites of cv-lysozyme 1 synthesis. Cv-lysozyme 1 enzyme activity measured by lysing
Micrococcus lysodeikticus bacteria and expressed in units per gram tissue was highest in mantles, labial palps and gills. Most cv-lysozyme 1 enzyme activity in oysters was found in plasma. Cv-lysozyme 1 main organs of synthesis, its abundance in plasma and its strong antimicrobial properties suggest its main role is in oyster host defences. |
| doi_str_mv | 10.1016/j.fsi.2007.03.006 |
| format | Article |
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Da lysozyme first purified from plasma of eastern oysters (
Crassostrea virginica) was identified and its complete amino acid sequence deduced. The amino acid sequence of the plasma lysozyme, designated cv-lysozyme 1, contained both a unique and a conserved region when compared to the amino acid sequences of other bivalve lysozymes.
In situ hybridisation located cv-lysozyme 1 gene expression in mantle and gill cells in standard histological sections. Quantitative real-time RT-PCR detected cv-lysozyme 1 expression in all organs examined and circulating haemocytes. The number of cv-lysozyme 1 mRNA transcripts was particularly high in mantles and labial palps suggesting those organs are the main sites of cv-lysozyme 1 synthesis. Cv-lysozyme 1 enzyme activity measured by lysing
Micrococcus lysodeikticus bacteria and expressed in units per gram tissue was highest in mantles, labial palps and gills. Most cv-lysozyme 1 enzyme activity in oysters was found in plasma. Cv-lysozyme 1 main organs of synthesis, its abundance in plasma and its strong antimicrobial properties suggest its main role is in oyster host defences.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.fsi.2007.03.006</identifier><identifier>PMID: 17703954</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Bivalve mollusc ; Bivalvia ; cDNA ; Cloning, Molecular ; Crassostrea - enzymology ; Crassostrea virginica ; Enzyme activity ; Gene expression ; Gene Expression Regulation ; Hemocytes - enzymology ; i-type lysozyme ; In situ hybridisation ; In Situ Hybridization - veterinary ; Invertebrate immunity ; Marine ; Micrococcus lysodeikticus ; Molecular Sequence Data ; Muramidase - chemistry ; Muramidase - genetics ; Oyster ; Quantitative real-time RT-PCR ; RNA, Messenger - metabolism ; Sequence Alignment ; Sequence Homology, Amino Acid</subject><ispartof>Fish & Shellfish Immunology, 2007-11, Vol.23 (5), p.957-968</ispartof><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-a8cd726b88beb296642bd4f44e92d881ec169c2968ada4d36fc63e021543fbb53</citedby><cites>FETCH-LOGICAL-c382t-a8cd726b88beb296642bd4f44e92d881ec169c2968ada4d36fc63e021543fbb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fsi.2007.03.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17703954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Itoh, Naoki</creatorcontrib><creatorcontrib>Xue, QingGang</creatorcontrib><creatorcontrib>Li, Yanli</creatorcontrib><creatorcontrib>Cooper, Richard K.</creatorcontrib><creatorcontrib>La Peyre, Jerome F.</creatorcontrib><title>cDNA cloning and tissue expression of plasma lysozyme in the eastern oyster, Crassostrea virginica</title><title>Fish & Shellfish Immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>The cDNA sequence of a 17,861
Da lysozyme first purified from plasma of eastern oysters (
Crassostrea virginica) was identified and its complete amino acid sequence deduced. The amino acid sequence of the plasma lysozyme, designated cv-lysozyme 1, contained both a unique and a conserved region when compared to the amino acid sequences of other bivalve lysozymes.
In situ hybridisation located cv-lysozyme 1 gene expression in mantle and gill cells in standard histological sections. Quantitative real-time RT-PCR detected cv-lysozyme 1 expression in all organs examined and circulating haemocytes. The number of cv-lysozyme 1 mRNA transcripts was particularly high in mantles and labial palps suggesting those organs are the main sites of cv-lysozyme 1 synthesis. Cv-lysozyme 1 enzyme activity measured by lysing
Micrococcus lysodeikticus bacteria and expressed in units per gram tissue was highest in mantles, labial palps and gills. Most cv-lysozyme 1 enzyme activity in oysters was found in plasma. Cv-lysozyme 1 main organs of synthesis, its abundance in plasma and its strong antimicrobial properties suggest its main role is in oyster host defences.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Bivalve mollusc</subject><subject>Bivalvia</subject><subject>cDNA</subject><subject>Cloning, Molecular</subject><subject>Crassostrea - enzymology</subject><subject>Crassostrea virginica</subject><subject>Enzyme activity</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Hemocytes - enzymology</subject><subject>i-type lysozyme</subject><subject>In situ hybridisation</subject><subject>In Situ Hybridization - veterinary</subject><subject>Invertebrate immunity</subject><subject>Marine</subject><subject>Micrococcus lysodeikticus</subject><subject>Molecular Sequence Data</subject><subject>Muramidase - chemistry</subject><subject>Muramidase - genetics</subject><subject>Oyster</subject><subject>Quantitative real-time RT-PCR</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><issn>1050-4648</issn><issn>1095-9947</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS1ERcvAD2CDvGJFUr_i2GJVTXlUqmADa8uxb4pHSTz4ZqoOv55EMxI7WJ0r3e-cxTmEvOGs5ozr613dY6oFY23NZM2YfkauOLNNZa1qn693wyqllbkkLxF3bCGkZi_IJW9bJm2jrkgXbr_e0DDkKU0P1E-RzgnxABSe9gUQU55o7ul-8Dh6Ohwx_z6OQNNE558L5HGGshDHVd_TbfGIGecCnj6m8pCmFPwrctH7AeH1WTfkx6eP37dfqvtvn--2N_dVkEbMlTchtkJ3xnTQCau1El1UvVJgRTSGQ-DahuVhfPQqSt0HLYEJ3ijZd10jN-TdKXdf8q8D4OzGhAGGwU-QD-i0ES3Xrf0vKFgrjLJ8AfkJDCUjFujdvqTRl6PjzK0LuJ1bFnDrAo5Jt_a7IW_P4YduhPjXca58AT6cAFi6eExQHIYEU4CYCoTZxZz-Ef8H8OqXow</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Itoh, Naoki</creator><creator>Xue, QingGang</creator><creator>Li, Yanli</creator><creator>Cooper, Richard K.</creator><creator>La Peyre, Jerome F.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>cDNA cloning and tissue expression of plasma lysozyme in the eastern oyster, Crassostrea virginica</title><author>Itoh, Naoki ; Xue, QingGang ; Li, Yanli ; Cooper, Richard K. ; La Peyre, Jerome F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-a8cd726b88beb296642bd4f44e92d881ec169c2968ada4d36fc63e021543fbb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Bivalve mollusc</topic><topic>Bivalvia</topic><topic>cDNA</topic><topic>Cloning, Molecular</topic><topic>Crassostrea - enzymology</topic><topic>Crassostrea virginica</topic><topic>Enzyme activity</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Hemocytes - enzymology</topic><topic>i-type lysozyme</topic><topic>In situ hybridisation</topic><topic>In Situ Hybridization - veterinary</topic><topic>Invertebrate immunity</topic><topic>Marine</topic><topic>Micrococcus lysodeikticus</topic><topic>Molecular Sequence Data</topic><topic>Muramidase - chemistry</topic><topic>Muramidase - genetics</topic><topic>Oyster</topic><topic>Quantitative real-time RT-PCR</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Itoh, Naoki</creatorcontrib><creatorcontrib>Xue, QingGang</creatorcontrib><creatorcontrib>Li, Yanli</creatorcontrib><creatorcontrib>Cooper, Richard K.</creatorcontrib><creatorcontrib>La Peyre, Jerome F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & Shellfish Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Itoh, Naoki</au><au>Xue, QingGang</au><au>Li, Yanli</au><au>Cooper, Richard K.</au><au>La Peyre, Jerome F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA cloning and tissue expression of plasma lysozyme in the eastern oyster, Crassostrea virginica</atitle><jtitle>Fish & Shellfish Immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>23</volume><issue>5</issue><spage>957</spage><epage>968</epage><pages>957-968</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><eissn>1365-2567</eissn><abstract>The cDNA sequence of a 17,861
Da lysozyme first purified from plasma of eastern oysters (
Crassostrea virginica) was identified and its complete amino acid sequence deduced. The amino acid sequence of the plasma lysozyme, designated cv-lysozyme 1, contained both a unique and a conserved region when compared to the amino acid sequences of other bivalve lysozymes.
In situ hybridisation located cv-lysozyme 1 gene expression in mantle and gill cells in standard histological sections. Quantitative real-time RT-PCR detected cv-lysozyme 1 expression in all organs examined and circulating haemocytes. The number of cv-lysozyme 1 mRNA transcripts was particularly high in mantles and labial palps suggesting those organs are the main sites of cv-lysozyme 1 synthesis. Cv-lysozyme 1 enzyme activity measured by lysing
Micrococcus lysodeikticus bacteria and expressed in units per gram tissue was highest in mantles, labial palps and gills. Most cv-lysozyme 1 enzyme activity in oysters was found in plasma. Cv-lysozyme 1 main organs of synthesis, its abundance in plasma and its strong antimicrobial properties suggest its main role is in oyster host defences.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>17703954</pmid><doi>10.1016/j.fsi.2007.03.006</doi><tpages>12</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Bivalve mollusc Bivalvia cDNA Cloning, Molecular Crassostrea - enzymology Crassostrea virginica Enzyme activity Gene expression Gene Expression Regulation Hemocytes - enzymology i-type lysozyme In situ hybridisation In Situ Hybridization - veterinary Invertebrate immunity Marine Micrococcus lysodeikticus Molecular Sequence Data Muramidase - chemistry Muramidase - genetics Oyster Quantitative real-time RT-PCR RNA, Messenger - metabolism Sequence Alignment Sequence Homology, Amino Acid |
| title | cDNA cloning and tissue expression of plasma lysozyme in the eastern oyster, Crassostrea virginica |
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