Ocular Surface Expression and In Vitro Activity of Antimicrobial Peptides
Purpose: Human ocular surface epithelia express four antimicrobial peptides (APs): β -defensin (hBD) 1-3 and LL-37. Here the expression of additional APs (hBD 4-6, HE2β 1; histatin-1, -3; liver expressed antimicrobial peptide-1, -2; macrophage inflammatory protein (MIP)-3α, and thymosin (T)β -4) was...
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| Veröffentlicht in: | Current eye research 2007-01, Vol.32 (7-8), p.595-609 |
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| creator | Huang, Ling C. Jean, Daniele Proske, Rita J. Reins, Rose Y. McDermott, Alison M. |
| description | Purpose: Human ocular surface epithelia express four antimicrobial peptides (APs): β -defensin (hBD) 1-3 and LL-37. Here the expression of additional APs (hBD 4-6, HE2β 1; histatin-1, -3; liver expressed antimicrobial peptide-1, -2; macrophage inflammatory protein (MIP)-3α, and thymosin (T)β -4) was sought and activity against common ocular pathogens studied. Methods: AP expression was determined in human corneal and conjunctival epithelial cells (HCEC, HCjEC) by RT-PCR and in corneal sections by immunostaining. Antimicrobial assays were performed to assess peptide (hBD 1-3, LL-37, MIP-3α, and Tβ 4) activity against Pseudomonas aeruginosa (PA), Staphylococcus aureus (SA), and Staphylococcus epidermidis (SE) in the presence of NaCl or tears. Results: HCEC and HCjEC expressed MIP-3α and Tβ 4. hBD 1-3, MIP-3α, and Tβ 4 showed activity against PA. hBD-3 had potent activity against SA and SE, whereas hBD-2, MIP-3α and Tβ 4 had moderate activity and hBD-1 had none. NaCl markedly attenuated, and tears almost completely inhibited the activity of hBD 1-2 and Tβ 4, but not that of hBD-3. Conclusions: The ocular surface epithelia additionally express MIP-3α and Tβ 4 which have moderate antimicrobial activity. The current data support a role for hBD-3 as an antimicrobial peptide in vivo, but call in to question the effectiveness of some other APs. However, further study is required to conclusively elucidate the physiological role of each AP. |
| doi_str_mv | 10.1080/02713680701446653 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_68268988</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68268988</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-ee7591fe913a18d8c4996b33709a195b595b2b7dc6d154c6bf70af83aab1c433</originalsourceid><addsrcrecordid>eNp9kE-LFDEQxYMo7rj6AbxITt5ak86fTqMIw7LqwMIKLl5DdTpxsqSTMUmvzre3hxnURdhDKEj93quqh9BLSt5Qoshb0naUSUU6QjmXUrBHaEW5JE3bkvYxWh36zQKwM_SslFtCDh_8KTqjnRItVWyFNtdmDpDx1zk7MBZf_tplW4pPEUMc8Sbib77mhNem-jtf9zg5vI7VT97kNHgI-IvdVT_a8hw9cRCKfXGq5-jm4-XNxefm6vrT5mJ91Rgu-tpY24meOttTBlSNyvC-lwNjHemB9mIQy2uHbjRypIIbObiOgFMMYKCGM3aOPhxtd_Mw2dHYWDMEvct-grzXCby-34l-q7-nO91yRgQVi8Hrk0FOP2Zbqp58MTYEiDbNRUvVStUrtYD0CC6XlpKt-zOEEn3IX_-X_6J59e92fxWnwBfg_RHw0aU8wc-Uw6gr7EPKLkM0vmj2kP-7e_KthVC3BrLVt2nOcQn-ge1-AzKPpqA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68268988</pqid></control><display><type>article</type><title>Ocular Surface Expression and In Vitro Activity of Antimicrobial Peptides</title><source>MEDLINE</source><source>Taylor & Francis Medical Library - CRKN</source><source>Taylor & Francis Journals Complete</source><creator>Huang, Ling C. ; Jean, Daniele ; Proske, Rita J. ; Reins, Rose Y. ; McDermott, Alison M.</creator><creatorcontrib>Huang, Ling C. ; Jean, Daniele ; Proske, Rita J. ; Reins, Rose Y. ; McDermott, Alison M.</creatorcontrib><description>Purpose: Human ocular surface epithelia express four antimicrobial peptides (APs): β -defensin (hBD) 1-3 and LL-37. Here the expression of additional APs (hBD 4-6, HE2β 1; histatin-1, -3; liver expressed antimicrobial peptide-1, -2; macrophage inflammatory protein (MIP)-3α, and thymosin (T)β -4) was sought and activity against common ocular pathogens studied. Methods: AP expression was determined in human corneal and conjunctival epithelial cells (HCEC, HCjEC) by RT-PCR and in corneal sections by immunostaining. Antimicrobial assays were performed to assess peptide (hBD 1-3, LL-37, MIP-3α, and Tβ 4) activity against Pseudomonas aeruginosa (PA), Staphylococcus aureus (SA), and Staphylococcus epidermidis (SE) in the presence of NaCl or tears. Results: HCEC and HCjEC expressed MIP-3α and Tβ 4. hBD 1-3, MIP-3α, and Tβ 4 showed activity against PA. hBD-3 had potent activity against SA and SE, whereas hBD-2, MIP-3α and Tβ 4 had moderate activity and hBD-1 had none. NaCl markedly attenuated, and tears almost completely inhibited the activity of hBD 1-2 and Tβ 4, but not that of hBD-3. Conclusions: The ocular surface epithelia additionally express MIP-3α and Tβ 4 which have moderate antimicrobial activity. The current data support a role for hBD-3 as an antimicrobial peptide in vivo, but call in to question the effectiveness of some other APs. However, further study is required to conclusively elucidate the physiological role of each AP.</description><identifier>ISSN: 0271-3683</identifier><identifier>EISSN: 1460-2202</identifier><identifier>DOI: 10.1080/02713680701446653</identifier><identifier>PMID: 17852183</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Adult ; Antimicrobial peptides ; beta-Defensins - genetics ; beta-Defensins - metabolism ; beta-Defensins - pharmacology ; cathelicidin ; Cell Culture Techniques ; Chemokine CCL20 ; Chemokines, CC - genetics ; Chemokines, CC - metabolism ; Chemokines, CC - pharmacology ; Colony Count, Microbial ; Conjunctiva - drug effects ; Conjunctiva - metabolism ; corneal epithelium ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Epithelium, Corneal - drug effects ; Epithelium, Corneal - metabolism ; Female ; Gene Expression - physiology ; Humans ; Interleukin-1beta - pharmacology ; Macrophage Inflammatory Proteins - genetics ; Macrophage Inflammatory Proteins - metabolism ; Macrophage Inflammatory Proteins - pharmacology ; Male ; Middle Aged ; MIP-3α/CCL20 ; ocular surface ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Sodium Chloride - pharmacology ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus epidermidis - drug effects ; Tears - physiology ; Thymosin - genetics ; Thymosin - metabolism ; Thymosin - pharmacology ; thymosin β-4 ; Tumor Necrosis Factor-alpha - pharmacology ; β-defensins</subject><ispartof>Current eye research, 2007-01, Vol.32 (7-8), p.595-609</ispartof><rights>2007 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-ee7591fe913a18d8c4996b33709a195b595b2b7dc6d154c6bf70af83aab1c433</citedby><cites>FETCH-LOGICAL-c459t-ee7591fe913a18d8c4996b33709a195b595b2b7dc6d154c6bf70af83aab1c433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/02713680701446653$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/02713680701446653$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17852183$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Ling C.</creatorcontrib><creatorcontrib>Jean, Daniele</creatorcontrib><creatorcontrib>Proske, Rita J.</creatorcontrib><creatorcontrib>Reins, Rose Y.</creatorcontrib><creatorcontrib>McDermott, Alison M.</creatorcontrib><title>Ocular Surface Expression and In Vitro Activity of Antimicrobial Peptides</title><title>Current eye research</title><addtitle>Curr Eye Res</addtitle><description>Purpose: Human ocular surface epithelia express four antimicrobial peptides (APs): β -defensin (hBD) 1-3 and LL-37. Here the expression of additional APs (hBD 4-6, HE2β 1; histatin-1, -3; liver expressed antimicrobial peptide-1, -2; macrophage inflammatory protein (MIP)-3α, and thymosin (T)β -4) was sought and activity against common ocular pathogens studied. Methods: AP expression was determined in human corneal and conjunctival epithelial cells (HCEC, HCjEC) by RT-PCR and in corneal sections by immunostaining. Antimicrobial assays were performed to assess peptide (hBD 1-3, LL-37, MIP-3α, and Tβ 4) activity against Pseudomonas aeruginosa (PA), Staphylococcus aureus (SA), and Staphylococcus epidermidis (SE) in the presence of NaCl or tears. Results: HCEC and HCjEC expressed MIP-3α and Tβ 4. hBD 1-3, MIP-3α, and Tβ 4 showed activity against PA. hBD-3 had potent activity against SA and SE, whereas hBD-2, MIP-3α and Tβ 4 had moderate activity and hBD-1 had none. NaCl markedly attenuated, and tears almost completely inhibited the activity of hBD 1-2 and Tβ 4, but not that of hBD-3. Conclusions: The ocular surface epithelia additionally express MIP-3α and Tβ 4 which have moderate antimicrobial activity. The current data support a role for hBD-3 as an antimicrobial peptide in vivo, but call in to question the effectiveness of some other APs. However, further study is required to conclusively elucidate the physiological role of each AP.</description><subject>Adult</subject><subject>Antimicrobial peptides</subject><subject>beta-Defensins - genetics</subject><subject>beta-Defensins - metabolism</subject><subject>beta-Defensins - pharmacology</subject><subject>cathelicidin</subject><subject>Cell Culture Techniques</subject><subject>Chemokine CCL20</subject><subject>Chemokines, CC - genetics</subject><subject>Chemokines, CC - metabolism</subject><subject>Chemokines, CC - pharmacology</subject><subject>Colony Count, Microbial</subject><subject>Conjunctiva - drug effects</subject><subject>Conjunctiva - metabolism</subject><subject>corneal epithelium</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelium, Corneal - drug effects</subject><subject>Epithelium, Corneal - metabolism</subject><subject>Female</subject><subject>Gene Expression - physiology</subject><subject>Humans</subject><subject>Interleukin-1beta - pharmacology</subject><subject>Macrophage Inflammatory Proteins - genetics</subject><subject>Macrophage Inflammatory Proteins - metabolism</subject><subject>Macrophage Inflammatory Proteins - pharmacology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>MIP-3α/CCL20</subject><subject>ocular surface</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Sodium Chloride - pharmacology</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus epidermidis - drug effects</subject><subject>Tears - physiology</subject><subject>Thymosin - genetics</subject><subject>Thymosin - metabolism</subject><subject>Thymosin - pharmacology</subject><subject>thymosin β-4</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><subject>β-defensins</subject><issn>0271-3683</issn><issn>1460-2202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE-LFDEQxYMo7rj6AbxITt5ak86fTqMIw7LqwMIKLl5DdTpxsqSTMUmvzre3hxnURdhDKEj93quqh9BLSt5Qoshb0naUSUU6QjmXUrBHaEW5JE3bkvYxWh36zQKwM_SslFtCDh_8KTqjnRItVWyFNtdmDpDx1zk7MBZf_tplW4pPEUMc8Sbib77mhNem-jtf9zg5vI7VT97kNHgI-IvdVT_a8hw9cRCKfXGq5-jm4-XNxefm6vrT5mJ91Rgu-tpY24meOttTBlSNyvC-lwNjHemB9mIQy2uHbjRypIIbObiOgFMMYKCGM3aOPhxtd_Mw2dHYWDMEvct-grzXCby-34l-q7-nO91yRgQVi8Hrk0FOP2Zbqp58MTYEiDbNRUvVStUrtYD0CC6XlpKt-zOEEn3IX_-X_6J59e92fxWnwBfg_RHw0aU8wc-Uw6gr7EPKLkM0vmj2kP-7e_KthVC3BrLVt2nOcQn-ge1-AzKPpqA</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Huang, Ling C.</creator><creator>Jean, Daniele</creator><creator>Proske, Rita J.</creator><creator>Reins, Rose Y.</creator><creator>McDermott, Alison M.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070101</creationdate><title>Ocular Surface Expression and In Vitro Activity of Antimicrobial Peptides</title><author>Huang, Ling C. ; Jean, Daniele ; Proske, Rita J. ; Reins, Rose Y. ; McDermott, Alison M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-ee7591fe913a18d8c4996b33709a195b595b2b7dc6d154c6bf70af83aab1c433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Antimicrobial peptides</topic><topic>beta-Defensins - genetics</topic><topic>beta-Defensins - metabolism</topic><topic>beta-Defensins - pharmacology</topic><topic>cathelicidin</topic><topic>Cell Culture Techniques</topic><topic>Chemokine CCL20</topic><topic>Chemokines, CC - genetics</topic><topic>Chemokines, CC - metabolism</topic><topic>Chemokines, CC - pharmacology</topic><topic>Colony Count, Microbial</topic><topic>Conjunctiva - drug effects</topic><topic>Conjunctiva - metabolism</topic><topic>corneal epithelium</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelium, Corneal - drug effects</topic><topic>Epithelium, Corneal - metabolism</topic><topic>Female</topic><topic>Gene Expression - physiology</topic><topic>Humans</topic><topic>Interleukin-1beta - pharmacology</topic><topic>Macrophage Inflammatory Proteins - genetics</topic><topic>Macrophage Inflammatory Proteins - metabolism</topic><topic>Macrophage Inflammatory Proteins - pharmacology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>MIP-3α/CCL20</topic><topic>ocular surface</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Sodium Chloride - pharmacology</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus epidermidis - drug effects</topic><topic>Tears - physiology</topic><topic>Thymosin - genetics</topic><topic>Thymosin - metabolism</topic><topic>Thymosin - pharmacology</topic><topic>thymosin β-4</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><topic>β-defensins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Ling C.</creatorcontrib><creatorcontrib>Jean, Daniele</creatorcontrib><creatorcontrib>Proske, Rita J.</creatorcontrib><creatorcontrib>Reins, Rose Y.</creatorcontrib><creatorcontrib>McDermott, Alison M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Current eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Ling C.</au><au>Jean, Daniele</au><au>Proske, Rita J.</au><au>Reins, Rose Y.</au><au>McDermott, Alison M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ocular Surface Expression and In Vitro Activity of Antimicrobial Peptides</atitle><jtitle>Current eye research</jtitle><addtitle>Curr Eye Res</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>32</volume><issue>7-8</issue><spage>595</spage><epage>609</epage><pages>595-609</pages><issn>0271-3683</issn><eissn>1460-2202</eissn><abstract>Purpose: Human ocular surface epithelia express four antimicrobial peptides (APs): β -defensin (hBD) 1-3 and LL-37. Here the expression of additional APs (hBD 4-6, HE2β 1; histatin-1, -3; liver expressed antimicrobial peptide-1, -2; macrophage inflammatory protein (MIP)-3α, and thymosin (T)β -4) was sought and activity against common ocular pathogens studied. Methods: AP expression was determined in human corneal and conjunctival epithelial cells (HCEC, HCjEC) by RT-PCR and in corneal sections by immunostaining. Antimicrobial assays were performed to assess peptide (hBD 1-3, LL-37, MIP-3α, and Tβ 4) activity against Pseudomonas aeruginosa (PA), Staphylococcus aureus (SA), and Staphylococcus epidermidis (SE) in the presence of NaCl or tears. Results: HCEC and HCjEC expressed MIP-3α and Tβ 4. hBD 1-3, MIP-3α, and Tβ 4 showed activity against PA. hBD-3 had potent activity against SA and SE, whereas hBD-2, MIP-3α and Tβ 4 had moderate activity and hBD-1 had none. NaCl markedly attenuated, and tears almost completely inhibited the activity of hBD 1-2 and Tβ 4, but not that of hBD-3. Conclusions: The ocular surface epithelia additionally express MIP-3α and Tβ 4 which have moderate antimicrobial activity. The current data support a role for hBD-3 as an antimicrobial peptide in vivo, but call in to question the effectiveness of some other APs. However, further study is required to conclusively elucidate the physiological role of each AP.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>17852183</pmid><doi>10.1080/02713680701446653</doi><tpages>15</tpages></addata></record> |
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| subjects | Adult Antimicrobial peptides beta-Defensins - genetics beta-Defensins - metabolism beta-Defensins - pharmacology cathelicidin Cell Culture Techniques Chemokine CCL20 Chemokines, CC - genetics Chemokines, CC - metabolism Chemokines, CC - pharmacology Colony Count, Microbial Conjunctiva - drug effects Conjunctiva - metabolism corneal epithelium Epithelial Cells - drug effects Epithelial Cells - metabolism Epithelium, Corneal - drug effects Epithelium, Corneal - metabolism Female Gene Expression - physiology Humans Interleukin-1beta - pharmacology Macrophage Inflammatory Proteins - genetics Macrophage Inflammatory Proteins - metabolism Macrophage Inflammatory Proteins - pharmacology Male Middle Aged MIP-3α/CCL20 ocular surface Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sodium Chloride - pharmacology Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus epidermidis - drug effects Tears - physiology Thymosin - genetics Thymosin - metabolism Thymosin - pharmacology thymosin β-4 Tumor Necrosis Factor-alpha - pharmacology β-defensins |
| title | Ocular Surface Expression and In Vitro Activity of Antimicrobial Peptides |
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