Differential effects of insulin-like growth factors on scratch wound repair in respiratory epithelial cells
Insulin-like growth factors (IGFs) I and II, being potent promoters of cellular growth and differentiation, were investigated for their effectiveness in improving the rate of scratch closure in human respiratory epithelium in vitro. Human epithelial cell lines from the nasal, bronchial, and tracheal...
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| Veröffentlicht in: | American journal of rhinology 2006-11, Vol.20 (6), p.652-657 |
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| creator | Adams, Damian H McIntosh, David Wormald, Peter-John Cowin, Allison J |
| description | Insulin-like growth factors (IGFs) I and II, being potent promoters of cellular growth and differentiation, were investigated for their effectiveness in improving the rate of scratch closure in human respiratory epithelium in vitro.
Human epithelial cell lines from the nasal, bronchial, and tracheal regions were analyzed for their response to IGF-I and IGF-II, in a confluent monolayer scratch assay. IGF-binding proteins (IGFBPs) produced by certain cells are able to reduce the effectiveness of the IGFs. Consequently, the analogues LongR3 IGF-I, Des1-3 IGF-I and Arg3 IGF-I were investigated also because of their lower affinity for the IGFBPs, while still retaining unaffected affinity for the IGF-I receptor.
All growth factors that were analyzed significantly improved the rate of scratch closure in bronchial and tracheal epithelial cells (p < or = 0.05). In comparison, scratch closure was markedly slower in nasal epithelial cells and IGF-I was the most effective growth factor at effecting scratch closure in these cells. The IGF-I analogues did not significantly improve scratch closure compared with IGF-I, despite the presence of IGFBP-3 in nasal, bronchial, and tracheal epithelial cells.
Addition of IGF-I to wounded nasal epithelial cells increases the rate of scratch closure and therefore may have potential for improving the healing of the nasal mucosa. |
| doi_str_mv | 10.2500/ajr.2006.20.2916 |
| format | Article |
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Human epithelial cell lines from the nasal, bronchial, and tracheal regions were analyzed for their response to IGF-I and IGF-II, in a confluent monolayer scratch assay. IGF-binding proteins (IGFBPs) produced by certain cells are able to reduce the effectiveness of the IGFs. Consequently, the analogues LongR3 IGF-I, Des1-3 IGF-I and Arg3 IGF-I were investigated also because of their lower affinity for the IGFBPs, while still retaining unaffected affinity for the IGF-I receptor.
All growth factors that were analyzed significantly improved the rate of scratch closure in bronchial and tracheal epithelial cells (p < or = 0.05). In comparison, scratch closure was markedly slower in nasal epithelial cells and IGF-I was the most effective growth factor at effecting scratch closure in these cells. The IGF-I analogues did not significantly improve scratch closure compared with IGF-I, despite the presence of IGFBP-3 in nasal, bronchial, and tracheal epithelial cells.
Addition of IGF-I to wounded nasal epithelial cells increases the rate of scratch closure and therefore may have potential for improving the healing of the nasal mucosa.</description><identifier>ISSN: 1050-6586</identifier><identifier>ISSN: 1945-8924</identifier><identifier>EISSN: 1539-6290</identifier><identifier>EISSN: 1945-8932</identifier><identifier>DOI: 10.2500/ajr.2006.20.2916</identifier><identifier>PMID: 17181112</identifier><language>eng</language><publisher>United States: SAGE PUBLICATIONS, INC</publisher><subject>Cell Line ; Cell Movement - drug effects ; Epithelial Cells - drug effects ; Epithelial Cells - pathology ; Humans ; Insulin-Like Growth Factor I - pharmacology ; Insulin-Like Growth Factor II - pharmacology ; Respiratory Mucosa - drug effects ; Respiratory Mucosa - injuries ; Respiratory Mucosa - pathology ; Wound Healing - drug effects ; Wounds and Injuries - drug therapy ; Wounds and Injuries - metabolism ; Wounds and Injuries - pathology</subject><ispartof>American journal of rhinology, 2006-11, Vol.20 (6), p.652-657</ispartof><rights>Copyright OceanSide Publications Nov 1, 2006</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c324t-4b67e4c69add29f55ff5bc79d2a0a42259f40d795d7af63c00cc179e6a08cd13</citedby><cites>FETCH-LOGICAL-c324t-4b67e4c69add29f55ff5bc79d2a0a42259f40d795d7af63c00cc179e6a08cd13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17181112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adams, Damian H</creatorcontrib><creatorcontrib>McIntosh, David</creatorcontrib><creatorcontrib>Wormald, Peter-John</creatorcontrib><creatorcontrib>Cowin, Allison J</creatorcontrib><title>Differential effects of insulin-like growth factors on scratch wound repair in respiratory epithelial cells</title><title>American journal of rhinology</title><addtitle>Am J Rhinol</addtitle><description>Insulin-like growth factors (IGFs) I and II, being potent promoters of cellular growth and differentiation, were investigated for their effectiveness in improving the rate of scratch closure in human respiratory epithelium in vitro.
Human epithelial cell lines from the nasal, bronchial, and tracheal regions were analyzed for their response to IGF-I and IGF-II, in a confluent monolayer scratch assay. IGF-binding proteins (IGFBPs) produced by certain cells are able to reduce the effectiveness of the IGFs. Consequently, the analogues LongR3 IGF-I, Des1-3 IGF-I and Arg3 IGF-I were investigated also because of their lower affinity for the IGFBPs, while still retaining unaffected affinity for the IGF-I receptor.
All growth factors that were analyzed significantly improved the rate of scratch closure in bronchial and tracheal epithelial cells (p < or = 0.05). In comparison, scratch closure was markedly slower in nasal epithelial cells and IGF-I was the most effective growth factor at effecting scratch closure in these cells. The IGF-I analogues did not significantly improve scratch closure compared with IGF-I, despite the presence of IGFBP-3 in nasal, bronchial, and tracheal epithelial cells.
Addition of IGF-I to wounded nasal epithelial cells increases the rate of scratch closure and therefore may have potential for improving the healing of the nasal mucosa.</description><subject>Cell Line</subject><subject>Cell Movement - drug effects</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - pathology</subject><subject>Humans</subject><subject>Insulin-Like Growth Factor I - pharmacology</subject><subject>Insulin-Like Growth Factor II - pharmacology</subject><subject>Respiratory Mucosa - drug effects</subject><subject>Respiratory Mucosa - injuries</subject><subject>Respiratory Mucosa - pathology</subject><subject>Wound Healing - drug effects</subject><subject>Wounds and Injuries - drug therapy</subject><subject>Wounds and Injuries - metabolism</subject><subject>Wounds and Injuries - pathology</subject><issn>1050-6586</issn><issn>1945-8924</issn><issn>1539-6290</issn><issn>1945-8932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkc9LwzAUx4Mobk7vniR48Nb5kjZpc5T5EwZedg9ZmrhsXVOTlrH_3hQHgpeXL7zP98sjX4RuCcwpA3hU2zCnADyNORWEn6EpYbnIOBVwnjQwyDir-ARdxbgFICWtyCWakJJUhBA6RbtnZ60Jpu2darBJWvcRe4tdG4fGtVnjdgZ_BX_oN9gq3fuQ1i2OOqheb_DBD22Ng-mUC8mTVOxcWvlwxKZz_cY0Y7A2TROv0YVVTTQ3p3eGVq8vq8V7tvx8-1g8LTOd06LPijUvTaG5UHVNhWXMWrbWpaipAlVQyoQtoC4Fq0tlea4BtCalMFxBpWuSz9DDb2wX_PdgYi_3Lo4HqNb4IUpeUU5L4Am8_wdu_RDadJqkOSSqIkWC4BfSwccYjJVdcHsVjpKAHEuQqQQ5lpCGHEtIlrtT7rDem_rPcPr1_Aep0YSa</recordid><startdate>200611</startdate><enddate>200611</enddate><creator>Adams, Damian H</creator><creator>McIntosh, David</creator><creator>Wormald, Peter-John</creator><creator>Cowin, Allison J</creator><general>SAGE PUBLICATIONS, INC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>200611</creationdate><title>Differential effects of insulin-like growth factors on scratch wound repair in respiratory epithelial cells</title><author>Adams, Damian H ; McIntosh, David ; Wormald, Peter-John ; Cowin, Allison J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c324t-4b67e4c69add29f55ff5bc79d2a0a42259f40d795d7af63c00cc179e6a08cd13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Cell Line</topic><topic>Cell Movement - drug effects</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - pathology</topic><topic>Humans</topic><topic>Insulin-Like Growth Factor I - pharmacology</topic><topic>Insulin-Like Growth Factor II - pharmacology</topic><topic>Respiratory Mucosa - drug effects</topic><topic>Respiratory Mucosa - injuries</topic><topic>Respiratory Mucosa - pathology</topic><topic>Wound Healing - drug effects</topic><topic>Wounds and Injuries - drug therapy</topic><topic>Wounds and Injuries - metabolism</topic><topic>Wounds and Injuries - pathology</topic><toplevel>online_resources</toplevel><creatorcontrib>Adams, Damian H</creatorcontrib><creatorcontrib>McIntosh, David</creatorcontrib><creatorcontrib>Wormald, Peter-John</creatorcontrib><creatorcontrib>Cowin, Allison J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of rhinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adams, Damian H</au><au>McIntosh, David</au><au>Wormald, Peter-John</au><au>Cowin, Allison J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential effects of insulin-like growth factors on scratch wound repair in respiratory epithelial cells</atitle><jtitle>American journal of rhinology</jtitle><addtitle>Am J Rhinol</addtitle><date>2006-11</date><risdate>2006</risdate><volume>20</volume><issue>6</issue><spage>652</spage><epage>657</epage><pages>652-657</pages><issn>1050-6586</issn><issn>1945-8924</issn><eissn>1539-6290</eissn><eissn>1945-8932</eissn><abstract>Insulin-like growth factors (IGFs) I and II, being potent promoters of cellular growth and differentiation, were investigated for their effectiveness in improving the rate of scratch closure in human respiratory epithelium in vitro.
Human epithelial cell lines from the nasal, bronchial, and tracheal regions were analyzed for their response to IGF-I and IGF-II, in a confluent monolayer scratch assay. IGF-binding proteins (IGFBPs) produced by certain cells are able to reduce the effectiveness of the IGFs. Consequently, the analogues LongR3 IGF-I, Des1-3 IGF-I and Arg3 IGF-I were investigated also because of their lower affinity for the IGFBPs, while still retaining unaffected affinity for the IGF-I receptor.
All growth factors that were analyzed significantly improved the rate of scratch closure in bronchial and tracheal epithelial cells (p < or = 0.05). In comparison, scratch closure was markedly slower in nasal epithelial cells and IGF-I was the most effective growth factor at effecting scratch closure in these cells. The IGF-I analogues did not significantly improve scratch closure compared with IGF-I, despite the presence of IGFBP-3 in nasal, bronchial, and tracheal epithelial cells.
Addition of IGF-I to wounded nasal epithelial cells increases the rate of scratch closure and therefore may have potential for improving the healing of the nasal mucosa.</abstract><cop>United States</cop><pub>SAGE PUBLICATIONS, INC</pub><pmid>17181112</pmid><doi>10.2500/ajr.2006.20.2916</doi><tpages>6</tpages></addata></record> |
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| subjects | Cell Line Cell Movement - drug effects Epithelial Cells - drug effects Epithelial Cells - pathology Humans Insulin-Like Growth Factor I - pharmacology Insulin-Like Growth Factor II - pharmacology Respiratory Mucosa - drug effects Respiratory Mucosa - injuries Respiratory Mucosa - pathology Wound Healing - drug effects Wounds and Injuries - drug therapy Wounds and Injuries - metabolism Wounds and Injuries - pathology |
| title | Differential effects of insulin-like growth factors on scratch wound repair in respiratory epithelial cells |
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