Expression Profiling of Human Donor Lungs to Understand Primary Graft Dysfunction After Lung Transplantation
Lung transplantation is the treatment of choice for end‐stage pulmonary diseases. A limited donor supply has resulted in 4000 patients on the waiting list. Currently, 10–20% of donor organs offered for transplantation are deemed suitable under the selection criteria, of which 15–25% fail due to prim...
Gespeichert in:
| Veröffentlicht in: | American journal of transplantation 2007-10, Vol.7 (10), p.2396-2405 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2405 |
|---|---|
| container_issue | 10 |
| container_start_page | 2396 |
| container_title | American journal of transplantation |
| container_volume | 7 |
| creator | Ray, M. Dharmarajan, S. Freudenberg, J. Zhang, W. Patterson, G. A. |
| description | Lung transplantation is the treatment of choice for end‐stage pulmonary diseases. A limited donor supply has resulted in 4000 patients on the waiting list. Currently, 10–20% of donor organs offered for transplantation are deemed suitable under the selection criteria, of which 15–25% fail due to primary graft dysfunction (PGD). This has spawned efforts to re‐examine the current selection criteria as well as search for alternative donor lungs selection criteria. In this study, we attempt to further our understanding of PGD by observing the changes in gene expression across donor lungs that developed PGD versus those that did not. From our analysis, we have obtained differentially expressed transcripts that were involved in signaling, apoptosis and stress‐activated pathways. Results also indicate that metallothionein 3 was over expressed in lungs that didn't develop PGD. This is the first such attempt to perform expression profiling of actual human lungs used for transplantation, for the identification of a molecular signature for PGD.
Analysis of gene expression differences between human donor lungs that develop primary graft dysfunction and those that do not identified genes and biological pathways that are highly correlated with primary graft dysfunction. |
| doi_str_mv | 10.1111/j.1600-6143.2007.01918.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68259984</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68259984</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5138-56c38022e4ce00f40477c062496d9ec22fd8d16255ea7d7838dea94f18a69e03</originalsourceid><addsrcrecordid>eNqNkE1PwjAYgBujEUT_gulFb5tvu6_u4IEAgoZED3huateSkdFiu0X4926OwNVe-ibv8349CGECIWnf0yYkKUCQkjgKKUAWAskJC_cXaHhKXJ7iKBmgG-83ACSjjF6jAclYnCRZNETVbL9zyvvSGvzhrC6r0qyx1XjRbIXBU2usw8vGrD2uLf40hXK-FqZo4XIr3AHPndA1nh68boysuzZjXau-Bq-cMH5XCVOLLnWLrrSovLo7_iO0epmtJotg-T5_nYyXgUxIxIIklREDSlUsFYCOIc4yCSmN87TIlaRUF6wgKU0SJbIiYxErlMhjTZhIcwXRCD32bXfOfjfK13xbeqmqdg9lG89TRpM8Z3ELsh6UznrvlOa7_ipOgHei-YZ3Dnnnk3ei-Z9ovm9L748zmq-tKs6FR7Mt8HAEhJei0q0JWfozlwNj0J47Qs8991NW6vDvBfj4bdVF0S_L_5nV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68259984</pqid></control><display><type>article</type><title>Expression Profiling of Human Donor Lungs to Understand Primary Graft Dysfunction After Lung Transplantation</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Ray, M. ; Dharmarajan, S. ; Freudenberg, J. ; Zhang, W. ; Patterson, G. A.</creator><creatorcontrib>Ray, M. ; Dharmarajan, S. ; Freudenberg, J. ; Zhang, W. ; Patterson, G. A.</creatorcontrib><description>Lung transplantation is the treatment of choice for end‐stage pulmonary diseases. A limited donor supply has resulted in 4000 patients on the waiting list. Currently, 10–20% of donor organs offered for transplantation are deemed suitable under the selection criteria, of which 15–25% fail due to primary graft dysfunction (PGD). This has spawned efforts to re‐examine the current selection criteria as well as search for alternative donor lungs selection criteria. In this study, we attempt to further our understanding of PGD by observing the changes in gene expression across donor lungs that developed PGD versus those that did not. From our analysis, we have obtained differentially expressed transcripts that were involved in signaling, apoptosis and stress‐activated pathways. Results also indicate that metallothionein 3 was over expressed in lungs that didn't develop PGD. This is the first such attempt to perform expression profiling of actual human lungs used for transplantation, for the identification of a molecular signature for PGD.
Analysis of gene expression differences between human donor lungs that develop primary graft dysfunction and those that do not identified genes and biological pathways that are highly correlated with primary graft dysfunction.</description><identifier>ISSN: 1600-6135</identifier><identifier>EISSN: 1600-6143</identifier><identifier>DOI: 10.1111/j.1600-6143.2007.01918.x</identifier><identifier>PMID: 17845573</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Biological and medical sciences ; Cadaver ; Cause of Death ; Female ; Gene Expression ; Gene Expression Profiling ; Gene pathway analysis ; human donor lungs ; Humans ; lung transplantation ; Lung Transplantation - pathology ; Male ; Medical sciences ; microarray gene expression ; Oligonucleotide Array Sequence Analysis ; Postoperative Complications - pathology ; primary graft dysfunction ; Retrospective Studies ; RNA - genetics ; RNA - isolation & purification ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tissue Donors - statistics & numerical data ; Transcription, Genetic ; Treatment Failure</subject><ispartof>American journal of transplantation, 2007-10, Vol.7 (10), p.2396-2405</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5138-56c38022e4ce00f40477c062496d9ec22fd8d16255ea7d7838dea94f18a69e03</citedby><cites>FETCH-LOGICAL-c5138-56c38022e4ce00f40477c062496d9ec22fd8d16255ea7d7838dea94f18a69e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-6143.2007.01918.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-6143.2007.01918.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19088051$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17845573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ray, M.</creatorcontrib><creatorcontrib>Dharmarajan, S.</creatorcontrib><creatorcontrib>Freudenberg, J.</creatorcontrib><creatorcontrib>Zhang, W.</creatorcontrib><creatorcontrib>Patterson, G. A.</creatorcontrib><title>Expression Profiling of Human Donor Lungs to Understand Primary Graft Dysfunction After Lung Transplantation</title><title>American journal of transplantation</title><addtitle>Am J Transplant</addtitle><description>Lung transplantation is the treatment of choice for end‐stage pulmonary diseases. A limited donor supply has resulted in 4000 patients on the waiting list. Currently, 10–20% of donor organs offered for transplantation are deemed suitable under the selection criteria, of which 15–25% fail due to primary graft dysfunction (PGD). This has spawned efforts to re‐examine the current selection criteria as well as search for alternative donor lungs selection criteria. In this study, we attempt to further our understanding of PGD by observing the changes in gene expression across donor lungs that developed PGD versus those that did not. From our analysis, we have obtained differentially expressed transcripts that were involved in signaling, apoptosis and stress‐activated pathways. Results also indicate that metallothionein 3 was over expressed in lungs that didn't develop PGD. This is the first such attempt to perform expression profiling of actual human lungs used for transplantation, for the identification of a molecular signature for PGD.
Analysis of gene expression differences between human donor lungs that develop primary graft dysfunction and those that do not identified genes and biological pathways that are highly correlated with primary graft dysfunction.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cadaver</subject><subject>Cause of Death</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gene pathway analysis</subject><subject>human donor lungs</subject><subject>Humans</subject><subject>lung transplantation</subject><subject>Lung Transplantation - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>microarray gene expression</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Postoperative Complications - pathology</subject><subject>primary graft dysfunction</subject><subject>Retrospective Studies</subject><subject>RNA - genetics</subject><subject>RNA - isolation & purification</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Tissue Donors - statistics & numerical data</subject><subject>Transcription, Genetic</subject><subject>Treatment Failure</subject><issn>1600-6135</issn><issn>1600-6143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1PwjAYgBujEUT_gulFb5tvu6_u4IEAgoZED3huateSkdFiu0X4926OwNVe-ibv8349CGECIWnf0yYkKUCQkjgKKUAWAskJC_cXaHhKXJ7iKBmgG-83ACSjjF6jAclYnCRZNETVbL9zyvvSGvzhrC6r0qyx1XjRbIXBU2usw8vGrD2uLf40hXK-FqZo4XIr3AHPndA1nh68boysuzZjXau-Bq-cMH5XCVOLLnWLrrSovLo7_iO0epmtJotg-T5_nYyXgUxIxIIklREDSlUsFYCOIc4yCSmN87TIlaRUF6wgKU0SJbIiYxErlMhjTZhIcwXRCD32bXfOfjfK13xbeqmqdg9lG89TRpM8Z3ELsh6UznrvlOa7_ipOgHei-YZ3Dnnnk3ei-Z9ovm9L748zmq-tKs6FR7Mt8HAEhJei0q0JWfozlwNj0J47Qs8991NW6vDvBfj4bdVF0S_L_5nV</recordid><startdate>200710</startdate><enddate>200710</enddate><creator>Ray, M.</creator><creator>Dharmarajan, S.</creator><creator>Freudenberg, J.</creator><creator>Zhang, W.</creator><creator>Patterson, G. A.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200710</creationdate><title>Expression Profiling of Human Donor Lungs to Understand Primary Graft Dysfunction After Lung Transplantation</title><author>Ray, M. ; Dharmarajan, S. ; Freudenberg, J. ; Zhang, W. ; Patterson, G. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5138-56c38022e4ce00f40477c062496d9ec22fd8d16255ea7d7838dea94f18a69e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cadaver</topic><topic>Cause of Death</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gene pathway analysis</topic><topic>human donor lungs</topic><topic>Humans</topic><topic>lung transplantation</topic><topic>Lung Transplantation - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>microarray gene expression</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Postoperative Complications - pathology</topic><topic>primary graft dysfunction</topic><topic>Retrospective Studies</topic><topic>RNA - genetics</topic><topic>RNA - isolation & purification</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Tissue Donors - statistics & numerical data</topic><topic>Transcription, Genetic</topic><topic>Treatment Failure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ray, M.</creatorcontrib><creatorcontrib>Dharmarajan, S.</creatorcontrib><creatorcontrib>Freudenberg, J.</creatorcontrib><creatorcontrib>Zhang, W.</creatorcontrib><creatorcontrib>Patterson, G. A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ray, M.</au><au>Dharmarajan, S.</au><au>Freudenberg, J.</au><au>Zhang, W.</au><au>Patterson, G. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression Profiling of Human Donor Lungs to Understand Primary Graft Dysfunction After Lung Transplantation</atitle><jtitle>American journal of transplantation</jtitle><addtitle>Am J Transplant</addtitle><date>2007-10</date><risdate>2007</risdate><volume>7</volume><issue>10</issue><spage>2396</spage><epage>2405</epage><pages>2396-2405</pages><issn>1600-6135</issn><eissn>1600-6143</eissn><abstract>Lung transplantation is the treatment of choice for end‐stage pulmonary diseases. A limited donor supply has resulted in 4000 patients on the waiting list. Currently, 10–20% of donor organs offered for transplantation are deemed suitable under the selection criteria, of which 15–25% fail due to primary graft dysfunction (PGD). This has spawned efforts to re‐examine the current selection criteria as well as search for alternative donor lungs selection criteria. In this study, we attempt to further our understanding of PGD by observing the changes in gene expression across donor lungs that developed PGD versus those that did not. From our analysis, we have obtained differentially expressed transcripts that were involved in signaling, apoptosis and stress‐activated pathways. Results also indicate that metallothionein 3 was over expressed in lungs that didn't develop PGD. This is the first such attempt to perform expression profiling of actual human lungs used for transplantation, for the identification of a molecular signature for PGD.
Analysis of gene expression differences between human donor lungs that develop primary graft dysfunction and those that do not identified genes and biological pathways that are highly correlated with primary graft dysfunction.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17845573</pmid><doi>10.1111/j.1600-6143.2007.01918.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1600-6135 |
| ispartof | American journal of transplantation, 2007-10, Vol.7 (10), p.2396-2405 |
| issn | 1600-6135 1600-6143 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68259984 |
| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences Cadaver Cause of Death Female Gene Expression Gene Expression Profiling Gene pathway analysis human donor lungs Humans lung transplantation Lung Transplantation - pathology Male Medical sciences microarray gene expression Oligonucleotide Array Sequence Analysis Postoperative Complications - pathology primary graft dysfunction Retrospective Studies RNA - genetics RNA - isolation & purification Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tissue Donors - statistics & numerical data Transcription, Genetic Treatment Failure |
| title | Expression Profiling of Human Donor Lungs to Understand Primary Graft Dysfunction After Lung Transplantation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T11%3A20%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20Profiling%20of%20Human%20Donor%20Lungs%20to%20Understand%20Primary%20Graft%20Dysfunction%20After%20Lung%20Transplantation&rft.jtitle=American%20journal%20of%20transplantation&rft.au=Ray,%20M.&rft.date=2007-10&rft.volume=7&rft.issue=10&rft.spage=2396&rft.epage=2405&rft.pages=2396-2405&rft.issn=1600-6135&rft.eissn=1600-6143&rft_id=info:doi/10.1111/j.1600-6143.2007.01918.x&rft_dat=%3Cproquest_cross%3E68259984%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68259984&rft_id=info:pmid/17845573&rfr_iscdi=true |