An improved rapid genotyping method for the study of beta-2 adrenergic receptor gene polymorphisms using single tube allele specific multiplex PCR
Summary Background: Seventeen single nucleotide polymorphisms (SNPs) have been identified so far, within the beta‐2 receptor (β2 AR) gene. The presence of so many SNPs within the β2 AR gene causes a problem, for those studying β2 AR pharmacogenetics, in relation to which SNPs to choose. Most of the...
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| Veröffentlicht in: | Journal of clinical pharmacy and therapeutics 2006-12, Vol.31 (6), p.637-640 |
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| container_title | Journal of clinical pharmacy and therapeutics |
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| creator | Zilfalil, B. A. Hoh, B. P. Nizam, M. Z. Liza-Sharmini, A. T. Teh, L. K. Ismail, R. |
| description | Summary
Background: Seventeen single nucleotide polymorphisms (SNPs) have been identified so far, within the beta‐2 receptor (β2 AR) gene. The presence of so many SNPs within the β2 AR gene causes a problem, for those studying β2 AR pharmacogenetics, in relation to which SNPs to choose. Most of the work has focused on the three common SNPs within the coding block (alleles 16, 27 and 164) and the techniques developed have been for these three functionally important alleles.
Objective: We report an improved polymerase chain reaction (PCR)‐based method for the simultaneous detection of five functionally important β2 AR alleles, namely beta 16A/G, beta utr−20C/T, beta 27C/G, beta utr−47C/T and beta 164C/T.
Methods: Genomic DNA was used as a template for duplex and triplex PCR to detect the polymorphic sites of the five alleles.
Result: DNA sequencing analysis confirmed the specificity of this PCR method.
Conclusion: This simplified single‐tube multiplexed PCR assay provides an easier, faster and more cost‐effective method than those available for studying the specified polymorphisms of the β2AR gene. |
| doi_str_mv | 10.1111/j.1365-2710.2006.00771.x |
| format | Article |
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Background: Seventeen single nucleotide polymorphisms (SNPs) have been identified so far, within the beta‐2 receptor (β2 AR) gene. The presence of so many SNPs within the β2 AR gene causes a problem, for those studying β2 AR pharmacogenetics, in relation to which SNPs to choose. Most of the work has focused on the three common SNPs within the coding block (alleles 16, 27 and 164) and the techniques developed have been for these three functionally important alleles.
Objective: We report an improved polymerase chain reaction (PCR)‐based method for the simultaneous detection of five functionally important β2 AR alleles, namely beta 16A/G, beta utr−20C/T, beta 27C/G, beta utr−47C/T and beta 164C/T.
Methods: Genomic DNA was used as a template for duplex and triplex PCR to detect the polymorphic sites of the five alleles.
Result: DNA sequencing analysis confirmed the specificity of this PCR method.
Conclusion: This simplified single‐tube multiplexed PCR assay provides an easier, faster and more cost‐effective method than those available for studying the specified polymorphisms of the β2AR gene.</description><identifier>ISSN: 0269-4727</identifier><identifier>EISSN: 1365-2710</identifier><identifier>DOI: 10.1111/j.1365-2710.2006.00771.x</identifier><identifier>PMID: 17176369</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>beta2-adrenergic receptor gene (β2 AR) ; Biological and medical sciences ; genetic polymorphism ; Genotype ; Medical sciences ; multiplex PCR ; Pharmacology. Drug treatments ; Polymerase Chain Reaction - methods ; Polymorphism, Genetic ; Receptors, Adrenergic, beta-2 - genetics</subject><ispartof>Journal of clinical pharmacy and therapeutics, 2006-12, Vol.31 (6), p.637-640</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4851-19046d6514752cd5fad34858a86c4d1a80ab84f53b3fdae231d2e072cec1aadb3</citedby><cites>FETCH-LOGICAL-c4851-19046d6514752cd5fad34858a86c4d1a80ab84f53b3fdae231d2e072cec1aadb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2710.2006.00771.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2710.2006.00771.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18314399$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17176369$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zilfalil, B. A.</creatorcontrib><creatorcontrib>Hoh, B. P.</creatorcontrib><creatorcontrib>Nizam, M. Z.</creatorcontrib><creatorcontrib>Liza-Sharmini, A. T.</creatorcontrib><creatorcontrib>Teh, L. K.</creatorcontrib><creatorcontrib>Ismail, R.</creatorcontrib><title>An improved rapid genotyping method for the study of beta-2 adrenergic receptor gene polymorphisms using single tube allele specific multiplex PCR</title><title>Journal of clinical pharmacy and therapeutics</title><addtitle>J Clin Pharm Ther</addtitle><description>Summary
Background: Seventeen single nucleotide polymorphisms (SNPs) have been identified so far, within the beta‐2 receptor (β2 AR) gene. The presence of so many SNPs within the β2 AR gene causes a problem, for those studying β2 AR pharmacogenetics, in relation to which SNPs to choose. Most of the work has focused on the three common SNPs within the coding block (alleles 16, 27 and 164) and the techniques developed have been for these three functionally important alleles.
Objective: We report an improved polymerase chain reaction (PCR)‐based method for the simultaneous detection of five functionally important β2 AR alleles, namely beta 16A/G, beta utr−20C/T, beta 27C/G, beta utr−47C/T and beta 164C/T.
Methods: Genomic DNA was used as a template for duplex and triplex PCR to detect the polymorphic sites of the five alleles.
Result: DNA sequencing analysis confirmed the specificity of this PCR method.
Conclusion: This simplified single‐tube multiplexed PCR assay provides an easier, faster and more cost‐effective method than those available for studying the specified polymorphisms of the β2AR gene.</description><subject>beta2-adrenergic receptor gene (β2 AR)</subject><subject>Biological and medical sciences</subject><subject>genetic polymorphism</subject><subject>Genotype</subject><subject>Medical sciences</subject><subject>multiplex PCR</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Genetic</subject><subject>Receptors, Adrenergic, beta-2 - genetics</subject><issn>0269-4727</issn><issn>1365-2710</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1z0zAQhjUMDA2Fv8DoAjcHfdiWc-DQCVDodKDQAEeNLK0TBfkDSYb4b_CLkUmmvaKDtKN93tVqX4QwJUua1qv9kvKyyJhIF4yQckmIEHR5eIAWd4mHaEFYucpywcQZehLCniRSMP4YnVFBRcnL1QL9ueiwbQff_wKDvRqswVvo-jgNttviFuKuN7jpPY47wCGOZsJ9g2uIKmNYGQ8d-K3V2IOGISYuqQEPvZva3g87G9qAxzDXmjcHOI41YOUcpDgMoG2T1O3ooh0cHPDN-stT9KhRLsCz03mOvr57u1m_z64_XX5YX1xnOq8KmtEVyUtTFjQXBdOmaJThKVGpqtS5oaoiqq7ypuA1b4wCxqlhQATToKlSpubn6OWxbvr9zxFClK0NGpxTHfRjkGXFiirnqwRWR1D7PgQPjRy8bZWfJCVy9kPu5Tx2OY9dzn7If37IQ5I-P70x1i2Ye-HJgAS8OAEqaOUarzptwz1XcZpamLnXR-63dTD9dwPyan2zSVHSZ0e9DREOd3rlf8hScFHI7x8v5Zv89hv_vLmVV_wv7bO4lw</recordid><startdate>200612</startdate><enddate>200612</enddate><creator>Zilfalil, B. A.</creator><creator>Hoh, B. P.</creator><creator>Nizam, M. Z.</creator><creator>Liza-Sharmini, A. T.</creator><creator>Teh, L. K.</creator><creator>Ismail, R.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200612</creationdate><title>An improved rapid genotyping method for the study of beta-2 adrenergic receptor gene polymorphisms using single tube allele specific multiplex PCR</title><author>Zilfalil, B. A. ; Hoh, B. P. ; Nizam, M. Z. ; Liza-Sharmini, A. T. ; Teh, L. K. ; Ismail, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4851-19046d6514752cd5fad34858a86c4d1a80ab84f53b3fdae231d2e072cec1aadb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>beta2-adrenergic receptor gene (β2 AR)</topic><topic>Biological and medical sciences</topic><topic>genetic polymorphism</topic><topic>Genotype</topic><topic>Medical sciences</topic><topic>multiplex PCR</topic><topic>Pharmacology. Drug treatments</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Genetic</topic><topic>Receptors, Adrenergic, beta-2 - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zilfalil, B. A.</creatorcontrib><creatorcontrib>Hoh, B. P.</creatorcontrib><creatorcontrib>Nizam, M. Z.</creatorcontrib><creatorcontrib>Liza-Sharmini, A. T.</creatorcontrib><creatorcontrib>Teh, L. K.</creatorcontrib><creatorcontrib>Ismail, R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical pharmacy and therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zilfalil, B. A.</au><au>Hoh, B. P.</au><au>Nizam, M. Z.</au><au>Liza-Sharmini, A. T.</au><au>Teh, L. K.</au><au>Ismail, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved rapid genotyping method for the study of beta-2 adrenergic receptor gene polymorphisms using single tube allele specific multiplex PCR</atitle><jtitle>Journal of clinical pharmacy and therapeutics</jtitle><addtitle>J Clin Pharm Ther</addtitle><date>2006-12</date><risdate>2006</risdate><volume>31</volume><issue>6</issue><spage>637</spage><epage>640</epage><pages>637-640</pages><issn>0269-4727</issn><eissn>1365-2710</eissn><abstract>Summary
Background: Seventeen single nucleotide polymorphisms (SNPs) have been identified so far, within the beta‐2 receptor (β2 AR) gene. The presence of so many SNPs within the β2 AR gene causes a problem, for those studying β2 AR pharmacogenetics, in relation to which SNPs to choose. Most of the work has focused on the three common SNPs within the coding block (alleles 16, 27 and 164) and the techniques developed have been for these three functionally important alleles.
Objective: We report an improved polymerase chain reaction (PCR)‐based method for the simultaneous detection of five functionally important β2 AR alleles, namely beta 16A/G, beta utr−20C/T, beta 27C/G, beta utr−47C/T and beta 164C/T.
Methods: Genomic DNA was used as a template for duplex and triplex PCR to detect the polymorphic sites of the five alleles.
Result: DNA sequencing analysis confirmed the specificity of this PCR method.
Conclusion: This simplified single‐tube multiplexed PCR assay provides an easier, faster and more cost‐effective method than those available for studying the specified polymorphisms of the β2AR gene.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17176369</pmid><doi>10.1111/j.1365-2710.2006.00771.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | beta2-adrenergic receptor gene (β2 AR) Biological and medical sciences genetic polymorphism Genotype Medical sciences multiplex PCR Pharmacology. Drug treatments Polymerase Chain Reaction - methods Polymorphism, Genetic Receptors, Adrenergic, beta-2 - genetics |
| title | An improved rapid genotyping method for the study of beta-2 adrenergic receptor gene polymorphisms using single tube allele specific multiplex PCR |
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