Signals in pathological CNS extracts of ALS mice promote hMSCs neurogenic differentiation in vitro
The capability of MSCs to differentiate into neurons has been proven by many studies. Recently, other studies have cast doubt on MSCs neurogenic differentiation with non-physiological chemical inducing agents in vitro. This present study was designed to use conditioned medium to investigate whether...
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| Veröffentlicht in: | Cell biology international 2007-11, Vol.31 (11), p.1428-1435 |
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| creator | Zhao, Cui-Ping Zhang, Cheng Wang, Yi-Hua Zhou, Sheng-Nian Zhou, Chang Li, Wan-Yi Yu, Mei-Juan |
| description | The capability of MSCs to differentiate into neurons has been proven by many studies. Recently, other studies have cast doubt on MSCs neurogenic differentiation with non-physiological chemical inducing agents in vitro. This present study was designed to use conditioned medium to investigate whether signals from pathological condition of ALS were competent to induce a program of neurogenic differentiation in expanded cultures of hMSCs. Incubation of hMSCs with conditioned medium prepared from CNS extracts of ALS mice (SOD1-G93A ALS mice) resulted in a time-dependent morphological change from fibroblast-like into neuron-like, concomitant with increase in the expression of Nestin and subsequent β-tubulin III, NSE and GAP43. Moreover, signals in pathological CNS extracts of ALS mice were more effective in promoting hMSCs neurogenic differentiation than those in physiological extracts of normal adult mice. These results show that pathological condition of ALS is endowed with capacity to induce hMSCs neurogenic differentiation and hMSCs have shown a potential candidate in cellular therapy for ALS. |
| doi_str_mv | 10.1016/j.cellbi.2007.06.003 |
| format | Article |
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Recently, other studies have cast doubt on MSCs neurogenic differentiation with non-physiological chemical inducing agents in vitro. This present study was designed to use conditioned medium to investigate whether signals from pathological condition of ALS were competent to induce a program of neurogenic differentiation in expanded cultures of hMSCs. Incubation of hMSCs with conditioned medium prepared from CNS extracts of ALS mice (SOD1-G93A ALS mice) resulted in a time-dependent morphological change from fibroblast-like into neuron-like, concomitant with increase in the expression of Nestin and subsequent β-tubulin III, NSE and GAP43. Moreover, signals in pathological CNS extracts of ALS mice were more effective in promoting hMSCs neurogenic differentiation than those in physiological extracts of normal adult mice. These results show that pathological condition of ALS is endowed with capacity to induce hMSCs neurogenic differentiation and hMSCs have shown a potential candidate in cellular therapy for ALS.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1016/j.cellbi.2007.06.003</identifier><identifier>PMID: 17703959</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Amyotrophic lateral sclerosis ; Amyotrophic Lateral Sclerosis - metabolism ; Animals ; Cell Differentiation ; Central Nervous System - metabolism ; Culture Media, Conditioned - metabolism ; Differentiation ; Female ; GAP-43 Protein - metabolism ; Humans ; Intermediate Filament Proteins - metabolism ; Male ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Mesenchyme ; Mice ; Mice, Transgenic ; Nerve Tissue Proteins - metabolism ; Nestin ; Stem cells ; Superoxide Dismutase - genetics ; Tubulin - metabolism</subject><ispartof>Cell biology international, 2007-11, Vol.31 (11), p.1428-1435</ispartof><rights>2007 International Federation for Cell Biology</rights><rights>The Author(s) Journal compilation © 2007 International Federation for Cell Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4491-ac4eefdc7c0d93a2e5064dcb38a77db4b153ea189c30cf0cceb85cfd9555dae03</citedby><cites>FETCH-LOGICAL-c4491-ac4eefdc7c0d93a2e5064dcb38a77db4b153ea189c30cf0cceb85cfd9555dae03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.cellbi.2007.06.003$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.cellbi.2007.06.003$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17703959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Cui-Ping</creatorcontrib><creatorcontrib>Zhang, Cheng</creatorcontrib><creatorcontrib>Wang, Yi-Hua</creatorcontrib><creatorcontrib>Zhou, Sheng-Nian</creatorcontrib><creatorcontrib>Zhou, Chang</creatorcontrib><creatorcontrib>Li, Wan-Yi</creatorcontrib><creatorcontrib>Yu, Mei-Juan</creatorcontrib><title>Signals in pathological CNS extracts of ALS mice promote hMSCs neurogenic differentiation in vitro</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>The capability of MSCs to differentiate into neurons has been proven by many studies. Recently, other studies have cast doubt on MSCs neurogenic differentiation with non-physiological chemical inducing agents in vitro. This present study was designed to use conditioned medium to investigate whether signals from pathological condition of ALS were competent to induce a program of neurogenic differentiation in expanded cultures of hMSCs. Incubation of hMSCs with conditioned medium prepared from CNS extracts of ALS mice (SOD1-G93A ALS mice) resulted in a time-dependent morphological change from fibroblast-like into neuron-like, concomitant with increase in the expression of Nestin and subsequent β-tubulin III, NSE and GAP43. Moreover, signals in pathological CNS extracts of ALS mice were more effective in promoting hMSCs neurogenic differentiation than those in physiological extracts of normal adult mice. These results show that pathological condition of ALS is endowed with capacity to induce hMSCs neurogenic differentiation and hMSCs have shown a potential candidate in cellular therapy for ALS.</description><subject>Amyotrophic lateral sclerosis</subject><subject>Amyotrophic Lateral Sclerosis - metabolism</subject><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Central Nervous System - metabolism</subject><subject>Culture Media, Conditioned - metabolism</subject><subject>Differentiation</subject><subject>Female</subject><subject>GAP-43 Protein - metabolism</subject><subject>Humans</subject><subject>Intermediate Filament Proteins - metabolism</subject><subject>Male</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchyme</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Nestin</subject><subject>Stem cells</subject><subject>Superoxide Dismutase - genetics</subject><subject>Tubulin - metabolism</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1v1DAQhi0EoqXwDxDyiVuCvYnzwQGpjaCttCyIFPVoOePJ1ksSL7a3tP8eR1nBDXHyHJ73Hc9DyGvOUs548W6XAg5DZ9IVY2XKipSx7Ak55awWSZUJ8XSeC5EUdS1OyAvvd4xxnlfFc3LCy5JltahPSdea7aQGT81E9yrc2cFuDaiBNpuW4kNwCoKntqfn65aOBpDunR1tQHr3uW08nfDg7BYnA1SbvkeHUzAqGDvNjfcmOPuSPOvjBnx1fM_I908fb5qrZP3l8ro5XyeQ5zVPFOSIvYYSmK4ztULBilxDl1WqLHWXd1xkqHhVQ8agZwDYVQJ6XQshtEKWnZG3S2_84c8D-iBH42dHakJ78LKoVkJwISKYLyA4673DXu6dGZV7lJzJ2a3cycWtnN1KVsjoNsbeHPsP3Yj6b-goMwLvF-CXGfDxv0plc3G9WcXzYzhZwsYHfPgTVu6HLMqsFPJ2cym_XuU331pxK5vIf1h4jErvDTrpweAEqI1DCFJb8-9zfgNAwK-5</recordid><startdate>200711</startdate><enddate>200711</enddate><creator>Zhao, Cui-Ping</creator><creator>Zhang, Cheng</creator><creator>Wang, Yi-Hua</creator><creator>Zhou, Sheng-Nian</creator><creator>Zhou, Chang</creator><creator>Li, Wan-Yi</creator><creator>Yu, Mei-Juan</creator><general>Elsevier Ltd</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200711</creationdate><title>Signals in pathological CNS extracts of ALS mice promote hMSCs neurogenic differentiation in vitro</title><author>Zhao, Cui-Ping ; Zhang, Cheng ; Wang, Yi-Hua ; Zhou, Sheng-Nian ; Zhou, Chang ; Li, Wan-Yi ; Yu, Mei-Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4491-ac4eefdc7c0d93a2e5064dcb38a77db4b153ea189c30cf0cceb85cfd9555dae03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amyotrophic lateral sclerosis</topic><topic>Amyotrophic Lateral Sclerosis - metabolism</topic><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Central Nervous System - metabolism</topic><topic>Culture Media, Conditioned - metabolism</topic><topic>Differentiation</topic><topic>Female</topic><topic>GAP-43 Protein - metabolism</topic><topic>Humans</topic><topic>Intermediate Filament Proteins - metabolism</topic><topic>Male</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchyme</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Nestin</topic><topic>Stem cells</topic><topic>Superoxide Dismutase - genetics</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Cui-Ping</creatorcontrib><creatorcontrib>Zhang, Cheng</creatorcontrib><creatorcontrib>Wang, Yi-Hua</creatorcontrib><creatorcontrib>Zhou, Sheng-Nian</creatorcontrib><creatorcontrib>Zhou, Chang</creatorcontrib><creatorcontrib>Li, Wan-Yi</creatorcontrib><creatorcontrib>Yu, Mei-Juan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Cui-Ping</au><au>Zhang, Cheng</au><au>Wang, Yi-Hua</au><au>Zhou, Sheng-Nian</au><au>Zhou, Chang</au><au>Li, Wan-Yi</au><au>Yu, Mei-Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Signals in pathological CNS extracts of ALS mice promote hMSCs neurogenic differentiation in vitro</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2007-11</date><risdate>2007</risdate><volume>31</volume><issue>11</issue><spage>1428</spage><epage>1435</epage><pages>1428-1435</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>The capability of MSCs to differentiate into neurons has been proven by many studies. Recently, other studies have cast doubt on MSCs neurogenic differentiation with non-physiological chemical inducing agents in vitro. This present study was designed to use conditioned medium to investigate whether signals from pathological condition of ALS were competent to induce a program of neurogenic differentiation in expanded cultures of hMSCs. Incubation of hMSCs with conditioned medium prepared from CNS extracts of ALS mice (SOD1-G93A ALS mice) resulted in a time-dependent morphological change from fibroblast-like into neuron-like, concomitant with increase in the expression of Nestin and subsequent β-tubulin III, NSE and GAP43. Moreover, signals in pathological CNS extracts of ALS mice were more effective in promoting hMSCs neurogenic differentiation than those in physiological extracts of normal adult mice. These results show that pathological condition of ALS is endowed with capacity to induce hMSCs neurogenic differentiation and hMSCs have shown a potential candidate in cellular therapy for ALS.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>17703959</pmid><doi>10.1016/j.cellbi.2007.06.003</doi><tpages>8</tpages></addata></record> |
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| subjects | Amyotrophic lateral sclerosis Amyotrophic Lateral Sclerosis - metabolism Animals Cell Differentiation Central Nervous System - metabolism Culture Media, Conditioned - metabolism Differentiation Female GAP-43 Protein - metabolism Humans Intermediate Filament Proteins - metabolism Male Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Mesenchyme Mice Mice, Transgenic Nerve Tissue Proteins - metabolism Nestin Stem cells Superoxide Dismutase - genetics Tubulin - metabolism |
| title | Signals in pathological CNS extracts of ALS mice promote hMSCs neurogenic differentiation in vitro |
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