A sphingosine kinase 1 mutation sensitizes the myocardium to ischemia/reperfusion injury
Sphingosine kinase (SphK) is a key enzyme in the synthesis of sphingosine 1-phosphate (S1P), a bioactive sphingolipid. SphK is involved in ischemic preconditioning (IPC). To date no studies in genetically altered animals have examined the role of SphK1 in myocardial ischemia/reperfusion (IR) injury...
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| Veröffentlicht in: | Cardiovascular research 2007-10, Vol.76 (1), p.41-50 |
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| creator | Jin, Zhu-Qiu Zhang, Jianqing Huang, Yong Hoover, Holly E Vessey, Donald A Karliner, Joel S |
| description | Sphingosine kinase (SphK) is a key enzyme in the synthesis of sphingosine 1-phosphate (S1P), a bioactive sphingolipid. SphK is involved in ischemic preconditioning (IPC). To date no studies in genetically altered animals have examined the role of SphK1 in myocardial ischemia/reperfusion (IR) injury and IPC.
Wild-type and SphK1 null mouse hearts were subjected to IR (50 min global ischemia and 40 min reperfusion) in a Langendorff apparatus. IPC consisted of 2 min of global ischemia and 2 min of reperfusion for two cycles. At baseline, there were no differences in left ventricular developed pressure (LVDP), +/-dP/dtmax, and LV end-diastolic pressure (EDP) between SphK1 mutant and wild-type (WT) mouse hearts. In the mutants, total SphK enzyme activity was reduced by 44% and S1P levels were decreased by 41%. SphK1 null hearts subjected to IR exhibited more cardiac damage compared with WT: LVDP and +/-dP/dtmax decreased, LVEDP increased, and infarct size increased (n=6, P |
| doi_str_mv | 10.1016/j.cardiores.2007.05.029 |
| format | Article |
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Wild-type and SphK1 null mouse hearts were subjected to IR (50 min global ischemia and 40 min reperfusion) in a Langendorff apparatus. IPC consisted of 2 min of global ischemia and 2 min of reperfusion for two cycles. At baseline, there were no differences in left ventricular developed pressure (LVDP), +/-dP/dtmax, and LV end-diastolic pressure (EDP) between SphK1 mutant and wild-type (WT) mouse hearts. In the mutants, total SphK enzyme activity was reduced by 44% and S1P levels were decreased by 41%. SphK1 null hearts subjected to IR exhibited more cardiac damage compared with WT: LVDP and +/-dP/dtmax decreased, LVEDP increased, and infarct size increased (n=6, P<0.05). Apoptosis was markedly enhanced in SphK1 mutant IR mouse hearts. IPC was cardioprotective in WT hearts, but this protection appeared to be ineffective in SphK1 null hearts. There was no change in infarct size in the IPC+IR group compared to the IR group in the null hearts (50.1+/-5.0% vs 45.0+/-3.8%, n=6, P=NS). IPC remained ineffective in the null hearts even when the index ischemia time was shortened by 10 min.
Deletion of the SphK1 gene sensitizes the myocardium to IR injury and appears to impair the protective effect of IPC. These data provide the first genetic evidence that the SphK1-S1P pathway is a critical mediator of IPC and cell survival.</description><identifier>ISSN: 0008-6363</identifier><identifier>DOI: 10.1016/j.cardiores.2007.05.029</identifier><identifier>PMID: 17610857</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Apoptosis ; Blotting, Western - methods ; Creatine Kinase - analysis ; Creatine Kinase - metabolism ; Disease Susceptibility ; In Situ Nick-End Labeling ; Ischemic Preconditioning, Myocardial ; Lysophospholipids - metabolism ; Mice ; Mice, Knockout ; Mutation ; Myocardium - enzymology ; Myocardium - pathology ; Perfusion ; Phosphotransferases (Alcohol Group Acceptor) - analysis ; Phosphotransferases (Alcohol Group Acceptor) - genetics ; Phosphotransferases (Alcohol Group Acceptor) - metabolism ; Reperfusion Injury - enzymology ; Reperfusion Injury - pathology ; Sphingosine - analogs & derivatives ; Sphingosine - metabolism</subject><ispartof>Cardiovascular research, 2007-10, Vol.76 (1), p.41-50</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17610857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jin, Zhu-Qiu</creatorcontrib><creatorcontrib>Zhang, Jianqing</creatorcontrib><creatorcontrib>Huang, Yong</creatorcontrib><creatorcontrib>Hoover, Holly E</creatorcontrib><creatorcontrib>Vessey, Donald A</creatorcontrib><creatorcontrib>Karliner, Joel S</creatorcontrib><title>A sphingosine kinase 1 mutation sensitizes the myocardium to ischemia/reperfusion injury</title><title>Cardiovascular research</title><addtitle>Cardiovasc Res</addtitle><description>Sphingosine kinase (SphK) is a key enzyme in the synthesis of sphingosine 1-phosphate (S1P), a bioactive sphingolipid. SphK is involved in ischemic preconditioning (IPC). To date no studies in genetically altered animals have examined the role of SphK1 in myocardial ischemia/reperfusion (IR) injury and IPC.
Wild-type and SphK1 null mouse hearts were subjected to IR (50 min global ischemia and 40 min reperfusion) in a Langendorff apparatus. IPC consisted of 2 min of global ischemia and 2 min of reperfusion for two cycles. At baseline, there were no differences in left ventricular developed pressure (LVDP), +/-dP/dtmax, and LV end-diastolic pressure (EDP) between SphK1 mutant and wild-type (WT) mouse hearts. In the mutants, total SphK enzyme activity was reduced by 44% and S1P levels were decreased by 41%. SphK1 null hearts subjected to IR exhibited more cardiac damage compared with WT: LVDP and +/-dP/dtmax decreased, LVEDP increased, and infarct size increased (n=6, P<0.05). Apoptosis was markedly enhanced in SphK1 mutant IR mouse hearts. IPC was cardioprotective in WT hearts, but this protection appeared to be ineffective in SphK1 null hearts. There was no change in infarct size in the IPC+IR group compared to the IR group in the null hearts (50.1+/-5.0% vs 45.0+/-3.8%, n=6, P=NS). IPC remained ineffective in the null hearts even when the index ischemia time was shortened by 10 min.
Deletion of the SphK1 gene sensitizes the myocardium to IR injury and appears to impair the protective effect of IPC. These data provide the first genetic evidence that the SphK1-S1P pathway is a critical mediator of IPC and cell survival.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Blotting, Western - methods</subject><subject>Creatine Kinase - analysis</subject><subject>Creatine Kinase - metabolism</subject><subject>Disease Susceptibility</subject><subject>In Situ Nick-End Labeling</subject><subject>Ischemic Preconditioning, Myocardial</subject><subject>Lysophospholipids - metabolism</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Mutation</subject><subject>Myocardium - enzymology</subject><subject>Myocardium - pathology</subject><subject>Perfusion</subject><subject>Phosphotransferases (Alcohol Group Acceptor) - analysis</subject><subject>Phosphotransferases (Alcohol Group Acceptor) - genetics</subject><subject>Phosphotransferases (Alcohol Group Acceptor) - metabolism</subject><subject>Reperfusion Injury - enzymology</subject><subject>Reperfusion Injury - pathology</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - metabolism</subject><issn>0008-6363</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kDtPwzAUhT2AaCn8BfDEltR2YicZq4qXVIkFJLbITm6oS20HP4by60mhTEdX97uPcxC6pSSnhIrlLu-k77XzEHJGSJUTnhPWnKE5IaTORCGKGboMYTeVnFflBZrRSlBS82qO3lc4jFttP1zQFvCntjIAptikKKN2FgewQUf9DQHHLWBzcL_XksHRYR26LRgtlx5G8EMKxwltd8kfrtD5IPcBrk-6QG8P96_rp2zz8vi8Xm2ykZEmZr2AiisySNXTgvEGBB9KMbUYp0Jy1fWqkWUJHRAmu2ryQpnqeSEUU1BTVizQ3d_e0buvBCG2ZvoK9ntpwaXQippNNDuCNycwKQN9O3ptpD-0_1kUPzQPY68</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Jin, Zhu-Qiu</creator><creator>Zhang, Jianqing</creator><creator>Huang, Yong</creator><creator>Hoover, Holly E</creator><creator>Vessey, Donald A</creator><creator>Karliner, Joel S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>A sphingosine kinase 1 mutation sensitizes the myocardium to ischemia/reperfusion injury</title><author>Jin, Zhu-Qiu ; Zhang, Jianqing ; Huang, Yong ; Hoover, Holly E ; Vessey, Donald A ; Karliner, Joel S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p209t-d6e75b0fabd13259e65f462092516a5bcdb9a44ece02ac736312bd536b2be8123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Blotting, Western - methods</topic><topic>Creatine Kinase - analysis</topic><topic>Creatine Kinase - metabolism</topic><topic>Disease Susceptibility</topic><topic>In Situ Nick-End Labeling</topic><topic>Ischemic Preconditioning, Myocardial</topic><topic>Lysophospholipids - metabolism</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Mutation</topic><topic>Myocardium - enzymology</topic><topic>Myocardium - pathology</topic><topic>Perfusion</topic><topic>Phosphotransferases (Alcohol Group Acceptor) - analysis</topic><topic>Phosphotransferases (Alcohol Group Acceptor) - genetics</topic><topic>Phosphotransferases (Alcohol Group Acceptor) - metabolism</topic><topic>Reperfusion Injury - enzymology</topic><topic>Reperfusion Injury - pathology</topic><topic>Sphingosine - analogs & derivatives</topic><topic>Sphingosine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Zhu-Qiu</creatorcontrib><creatorcontrib>Zhang, Jianqing</creatorcontrib><creatorcontrib>Huang, Yong</creatorcontrib><creatorcontrib>Hoover, Holly E</creatorcontrib><creatorcontrib>Vessey, Donald A</creatorcontrib><creatorcontrib>Karliner, Joel S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cardiovascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, Zhu-Qiu</au><au>Zhang, Jianqing</au><au>Huang, Yong</au><au>Hoover, Holly E</au><au>Vessey, Donald A</au><au>Karliner, Joel S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sphingosine kinase 1 mutation sensitizes the myocardium to ischemia/reperfusion injury</atitle><jtitle>Cardiovascular research</jtitle><addtitle>Cardiovasc Res</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>76</volume><issue>1</issue><spage>41</spage><epage>50</epage><pages>41-50</pages><issn>0008-6363</issn><abstract>Sphingosine kinase (SphK) is a key enzyme in the synthesis of sphingosine 1-phosphate (S1P), a bioactive sphingolipid. SphK is involved in ischemic preconditioning (IPC). To date no studies in genetically altered animals have examined the role of SphK1 in myocardial ischemia/reperfusion (IR) injury and IPC.
Wild-type and SphK1 null mouse hearts were subjected to IR (50 min global ischemia and 40 min reperfusion) in a Langendorff apparatus. IPC consisted of 2 min of global ischemia and 2 min of reperfusion for two cycles. At baseline, there were no differences in left ventricular developed pressure (LVDP), +/-dP/dtmax, and LV end-diastolic pressure (EDP) between SphK1 mutant and wild-type (WT) mouse hearts. In the mutants, total SphK enzyme activity was reduced by 44% and S1P levels were decreased by 41%. SphK1 null hearts subjected to IR exhibited more cardiac damage compared with WT: LVDP and +/-dP/dtmax decreased, LVEDP increased, and infarct size increased (n=6, P<0.05). Apoptosis was markedly enhanced in SphK1 mutant IR mouse hearts. IPC was cardioprotective in WT hearts, but this protection appeared to be ineffective in SphK1 null hearts. There was no change in infarct size in the IPC+IR group compared to the IR group in the null hearts (50.1+/-5.0% vs 45.0+/-3.8%, n=6, P=NS). IPC remained ineffective in the null hearts even when the index ischemia time was shortened by 10 min.
Deletion of the SphK1 gene sensitizes the myocardium to IR injury and appears to impair the protective effect of IPC. These data provide the first genetic evidence that the SphK1-S1P pathway is a critical mediator of IPC and cell survival.</abstract><cop>England</cop><pmid>17610857</pmid><doi>10.1016/j.cardiores.2007.05.029</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Apoptosis Blotting, Western - methods Creatine Kinase - analysis Creatine Kinase - metabolism Disease Susceptibility In Situ Nick-End Labeling Ischemic Preconditioning, Myocardial Lysophospholipids - metabolism Mice Mice, Knockout Mutation Myocardium - enzymology Myocardium - pathology Perfusion Phosphotransferases (Alcohol Group Acceptor) - analysis Phosphotransferases (Alcohol Group Acceptor) - genetics Phosphotransferases (Alcohol Group Acceptor) - metabolism Reperfusion Injury - enzymology Reperfusion Injury - pathology Sphingosine - analogs & derivatives Sphingosine - metabolism |
| title | A sphingosine kinase 1 mutation sensitizes the myocardium to ischemia/reperfusion injury |
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