In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn, Macrobrachium rosenbergii (de Man), in Taiwan
Between January and March 2006, suspected outbreaks of white tail disease were observed in post‐larvae and juveniles of Macrobrachium rosenbergii in hatcheries and nursery ponds at Kaohsiung and Pingtung Counties in southern Taiwan. Pathognomonic lesions showed the presence of large oval and/or irre...
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| Veröffentlicht in: | Journal of fish diseases 2006-11, Vol.29 (11), p.665-671 |
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| container_title | Journal of fish diseases |
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| creator | Hsieh, C-Y Wu, Z-B Tung, M-C Tu, C Lo, S-P Chang, T-C Chang, C-D Chen, S-C Hsieh, Y-C Tsai, S-S |
| description | Between January and March 2006, suspected outbreaks of white tail disease were observed in post‐larvae and juveniles of Macrobrachium rosenbergii in hatcheries and nursery ponds at Kaohsiung and Pingtung Counties in southern Taiwan. Pathognomonic lesions showed the presence of large oval and/or irregular basophilic, cytoplasmic inclusion bodies in the infected muscles and hepatopancreas. Reverse transcriptase polymerase chain reaction (RT‐PCR) assay and in situ hybridization (ISH) revealed evidence of M. rosenbergii nodavirus (MrNV) infection but did not detect extra small virus. Phylogenetic analysis indicated that there were very high identities between nucleotide sequences among six strains obtained in this study (99.5–100%), moderate identities with Caribbean and Indian strains (98.2–98.6%), but slightly lower identity with a Chinese strain (95.2%). This is the first confirmation of MrNV in giant freshwater prawns, using an RT‐PCR and ISH, in Taiwan. |
| doi_str_mv | 10.1111/j.1365-2761.2006.00762.x |
| format | Article |
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Pathognomonic lesions showed the presence of large oval and/or irregular basophilic, cytoplasmic inclusion bodies in the infected muscles and hepatopancreas. Reverse transcriptase polymerase chain reaction (RT‐PCR) assay and in situ hybridization (ISH) revealed evidence of M. rosenbergii nodavirus (MrNV) infection but did not detect extra small virus. Phylogenetic analysis indicated that there were very high identities between nucleotide sequences among six strains obtained in this study (99.5–100%), moderate identities with Caribbean and Indian strains (98.2–98.6%), but slightly lower identity with a Chinese strain (95.2%). This is the first confirmation of MrNV in giant freshwater prawns, using an RT‐PCR and ISH, in Taiwan.</description><identifier>ISSN: 0140-7775</identifier><identifier>EISSN: 1365-2761</identifier><identifier>DOI: 10.1111/j.1365-2761.2006.00762.x</identifier><identifier>PMID: 17169113</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Abdominal Muscles - pathology ; Animals ; detection ; DNA-Directed RNA Polymerases - genetics ; Freshwater ; giant freshwater prawn ; Hepatopancreas - pathology ; In Situ Hybridization ; Macrobrachium rosenbergii ; Macrobrachium rosenbergii nodavirus (MrNV) ; Molecular Sequence Data ; Nodaviridae - classification ; Nodaviridae - genetics ; Nodaviridae - isolation & purification ; Nodaviridae - pathogenicity ; Nodavirus ; Palaemonidae - virology ; Phylogeny ; polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - chemistry ; Sequence Analysis, DNA ; Taiwan</subject><ispartof>Journal of fish diseases, 2006-11, Vol.29 (11), p.665-671</ispartof><rights>2006 The Authors. Journal compilation 2006 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4622-fcc571aa1d1590260af417a45f57ae0971823a72d886071154a82ed8eb438f773</citedby><cites>FETCH-LOGICAL-c4622-fcc571aa1d1590260af417a45f57ae0971823a72d886071154a82ed8eb438f773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2761.2006.00762.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2761.2006.00762.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17169113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsieh, C-Y</creatorcontrib><creatorcontrib>Wu, Z-B</creatorcontrib><creatorcontrib>Tung, M-C</creatorcontrib><creatorcontrib>Tu, C</creatorcontrib><creatorcontrib>Lo, S-P</creatorcontrib><creatorcontrib>Chang, T-C</creatorcontrib><creatorcontrib>Chang, C-D</creatorcontrib><creatorcontrib>Chen, S-C</creatorcontrib><creatorcontrib>Hsieh, Y-C</creatorcontrib><creatorcontrib>Tsai, S-S</creatorcontrib><title>In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn, Macrobrachium rosenbergii (de Man), in Taiwan</title><title>Journal of fish diseases</title><addtitle>J Fish Dis</addtitle><description>Between January and March 2006, suspected outbreaks of white tail disease were observed in post‐larvae and juveniles of Macrobrachium rosenbergii in hatcheries and nursery ponds at Kaohsiung and Pingtung Counties in southern Taiwan. Pathognomonic lesions showed the presence of large oval and/or irregular basophilic, cytoplasmic inclusion bodies in the infected muscles and hepatopancreas. Reverse transcriptase polymerase chain reaction (RT‐PCR) assay and in situ hybridization (ISH) revealed evidence of M. rosenbergii nodavirus (MrNV) infection but did not detect extra small virus. Phylogenetic analysis indicated that there were very high identities between nucleotide sequences among six strains obtained in this study (99.5–100%), moderate identities with Caribbean and Indian strains (98.2–98.6%), but slightly lower identity with a Chinese strain (95.2%). This is the first confirmation of MrNV in giant freshwater prawns, using an RT‐PCR and ISH, in Taiwan.</description><subject>Abdominal Muscles - pathology</subject><subject>Animals</subject><subject>detection</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Freshwater</subject><subject>giant freshwater prawn</subject><subject>Hepatopancreas - pathology</subject><subject>In Situ Hybridization</subject><subject>Macrobrachium rosenbergii</subject><subject>Macrobrachium rosenbergii nodavirus (MrNV)</subject><subject>Molecular Sequence Data</subject><subject>Nodaviridae - classification</subject><subject>Nodaviridae - genetics</subject><subject>Nodaviridae - isolation & purification</subject><subject>Nodaviridae - pathogenicity</subject><subject>Nodavirus</subject><subject>Palaemonidae - virology</subject><subject>Phylogeny</subject><subject>polymerase chain reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - chemistry</subject><subject>Sequence Analysis, DNA</subject><subject>Taiwan</subject><issn>0140-7775</issn><issn>1365-2761</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdFu0zAUhiMEYt3gFZDFBWLSEnyc2E6k3aBCy9iAaSri0jpJnNWldYqd0JZH4Wlx1mpIXAC-seXzfb9k_1FEgCYQ1qtFAqngMZMCEkapSCiVgiXbB9HofvAwGlHIaCyl5EfRsfcLSkFyEI-jI5AgCoB0FP28sMSbrifzXelMbX5gZ1pL0NbkZhZfj29IrTtd3V22DfmAlWtLh9Xc9CviWq9tqd2tMcS2NX43rvfEWHJr0HakcdrPN9hpR9YON_bsL_rLWoepPT0b9BmaDdon0aMGl14_Pewn0efJ29n4XXz1aXoxfn0VV5lgLG6qiktAhBp4QZmg2GQgMeMNl6hpISFnKUpW57mgEoBnmDNd57rM0ryRMj2JXuxz16791mvfqZXxlV4u0eq290rkjKdA6T9BKLiAIs8C-PwPcNH2zoZHKEZ5VqShwwDleyh8ifdON2rtzArdTgFVQ8tqoYYy1VCmGlpWdy2rbVCfHfL7cqXr3-Kh1gCc74GNWerdfwer95M34RD0eK8b3-ntvY7uqxIylVx9-ThVk-yaycvpTF2mvwAvO8S3</recordid><startdate>200611</startdate><enddate>200611</enddate><creator>Hsieh, C-Y</creator><creator>Wu, Z-B</creator><creator>Tung, M-C</creator><creator>Tu, C</creator><creator>Lo, S-P</creator><creator>Chang, T-C</creator><creator>Chang, C-D</creator><creator>Chen, S-C</creator><creator>Hsieh, Y-C</creator><creator>Tsai, S-S</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200611</creationdate><title>In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn, Macrobrachium rosenbergii (de Man), in Taiwan</title><author>Hsieh, C-Y ; Wu, Z-B ; Tung, M-C ; Tu, C ; Lo, S-P ; Chang, T-C ; Chang, C-D ; Chen, S-C ; Hsieh, Y-C ; Tsai, S-S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4622-fcc571aa1d1590260af417a45f57ae0971823a72d886071154a82ed8eb438f773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Abdominal Muscles - pathology</topic><topic>Animals</topic><topic>detection</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Freshwater</topic><topic>giant freshwater prawn</topic><topic>Hepatopancreas - pathology</topic><topic>In Situ Hybridization</topic><topic>Macrobrachium rosenbergii</topic><topic>Macrobrachium rosenbergii nodavirus (MrNV)</topic><topic>Molecular Sequence Data</topic><topic>Nodaviridae - classification</topic><topic>Nodaviridae - genetics</topic><topic>Nodaviridae - isolation & purification</topic><topic>Nodaviridae - pathogenicity</topic><topic>Nodavirus</topic><topic>Palaemonidae - virology</topic><topic>Phylogeny</topic><topic>polymerase chain reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - chemistry</topic><topic>Sequence Analysis, DNA</topic><topic>Taiwan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsieh, C-Y</creatorcontrib><creatorcontrib>Wu, Z-B</creatorcontrib><creatorcontrib>Tung, M-C</creatorcontrib><creatorcontrib>Tu, C</creatorcontrib><creatorcontrib>Lo, S-P</creatorcontrib><creatorcontrib>Chang, T-C</creatorcontrib><creatorcontrib>Chang, C-D</creatorcontrib><creatorcontrib>Chen, S-C</creatorcontrib><creatorcontrib>Hsieh, Y-C</creatorcontrib><creatorcontrib>Tsai, S-S</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fish diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsieh, C-Y</au><au>Wu, Z-B</au><au>Tung, M-C</au><au>Tu, C</au><au>Lo, S-P</au><au>Chang, T-C</au><au>Chang, C-D</au><au>Chen, S-C</au><au>Hsieh, Y-C</au><au>Tsai, S-S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn, Macrobrachium rosenbergii (de Man), in Taiwan</atitle><jtitle>Journal of fish diseases</jtitle><addtitle>J Fish Dis</addtitle><date>2006-11</date><risdate>2006</risdate><volume>29</volume><issue>11</issue><spage>665</spage><epage>671</epage><pages>665-671</pages><issn>0140-7775</issn><eissn>1365-2761</eissn><abstract>Between January and March 2006, suspected outbreaks of white tail disease were observed in post‐larvae and juveniles of Macrobrachium rosenbergii in hatcheries and nursery ponds at Kaohsiung and Pingtung Counties in southern Taiwan. Pathognomonic lesions showed the presence of large oval and/or irregular basophilic, cytoplasmic inclusion bodies in the infected muscles and hepatopancreas. Reverse transcriptase polymerase chain reaction (RT‐PCR) assay and in situ hybridization (ISH) revealed evidence of M. rosenbergii nodavirus (MrNV) infection but did not detect extra small virus. Phylogenetic analysis indicated that there were very high identities between nucleotide sequences among six strains obtained in this study (99.5–100%), moderate identities with Caribbean and Indian strains (98.2–98.6%), but slightly lower identity with a Chinese strain (95.2%). This is the first confirmation of MrNV in giant freshwater prawns, using an RT‐PCR and ISH, in Taiwan.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17169113</pmid><doi>10.1111/j.1365-2761.2006.00762.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of fish diseases, 2006-11, Vol.29 (11), p.665-671 |
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| subjects | Abdominal Muscles - pathology Animals detection DNA-Directed RNA Polymerases - genetics Freshwater giant freshwater prawn Hepatopancreas - pathology In Situ Hybridization Macrobrachium rosenbergii Macrobrachium rosenbergii nodavirus (MrNV) Molecular Sequence Data Nodaviridae - classification Nodaviridae - genetics Nodaviridae - isolation & purification Nodaviridae - pathogenicity Nodavirus Palaemonidae - virology Phylogeny polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - chemistry Sequence Analysis, DNA Taiwan |
| title | In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn, Macrobrachium rosenbergii (de Man), in Taiwan |
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