Nanometer-Scale Immobilization of Polysaccharides on Hydrophobic Polymer Plates in Supercritical Fluoroform/Water Emulsions
Hydrophilic polysaccharides such as dextran and hyaluronan were immobilized on a hydrophobic polystyrene (PSt) plate by a nanometer-scale surface penetration method in the emulsion of aqueous solutions in supercritical fluoroform (scCHF3). Since a supercritical fluid has high diffusiveness, water em...
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Veröffentlicht in: | Biomacromolecules 2007-09, Vol.8 (9), p.2815-2820 |
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creator | Mori, Toshiaki Sekine, Yoshimi Hasegawa, Mirei Okahata, Yoshio |
description | Hydrophilic polysaccharides such as dextran and hyaluronan were immobilized on a hydrophobic polystyrene (PSt) plate by a nanometer-scale surface penetration method in the emulsion of aqueous solutions in supercritical fluoroform (scCHF3). Since a supercritical fluid has high diffusiveness, water emulsions of polysaccharides can penetrate into the polymer surface. Dextran was surface-penetrated by two different methods: (1) the penetration of sucrose as a glucose donor and then the enzymatic polymerization to dextran near the surface catalyzed by dextransucrase, and (2) the direct penetration of dextran polymer into the PSt plate. The contact angle for water of the dextran-penetrated PSt plate was decreased to 78° from 95° of the untreated plate. The surface coverage and the penetration depth of polysaccharides could be obtained to be 10−30% and 10−20 nm, respectively, by X-ray photoelectron spectroscopy. These values could be controlled by the pressure of scCHF3. The transparency of the PSt dish did not change after the dextran penetration. Dextrans on the PSt plate could be elongated enzymatically by dextransucrase in the presence of sucrose as a glucose donor, and be detected by the enzyme-linked biotin−avidin assay. When anionic hyaluronan was surface-penetrated on the PSt plate instead of the neutral dextran, the plate showed the specific adhesion for human T-cells having hyaluronan receptors. |
doi_str_mv | 10.1021/bm7005467 |
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Since a supercritical fluid has high diffusiveness, water emulsions of polysaccharides can penetrate into the polymer surface. Dextran was surface-penetrated by two different methods: (1) the penetration of sucrose as a glucose donor and then the enzymatic polymerization to dextran near the surface catalyzed by dextransucrase, and (2) the direct penetration of dextran polymer into the PSt plate. The contact angle for water of the dextran-penetrated PSt plate was decreased to 78° from 95° of the untreated plate. The surface coverage and the penetration depth of polysaccharides could be obtained to be 10−30% and 10−20 nm, respectively, by X-ray photoelectron spectroscopy. These values could be controlled by the pressure of scCHF3. The transparency of the PSt dish did not change after the dextran penetration. Dextrans on the PSt plate could be elongated enzymatically by dextransucrase in the presence of sucrose as a glucose donor, and be detected by the enzyme-linked biotin−avidin assay. When anionic hyaluronan was surface-penetrated on the PSt plate instead of the neutral dextran, the plate showed the specific adhesion for human T-cells having hyaluronan receptors.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/bm7005467</identifier><identifier>PMID: 17715963</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Applied sciences ; Cells, Immobilized ; Chlorofluorocarbons - chemistry ; Dextrans - chemistry ; Emulsions - chemistry ; Exact sciences and technology ; Fluorocarbons - chemistry ; Humans ; Hyaluronic Acid - chemistry ; Hydrophobic and Hydrophilic Interactions ; Nanostructures ; Organic polymers ; Physicochemistry of polymers ; Polymers - chemistry ; Polysaccharides - chemistry ; Properties and characterization ; Special properties (catalyst, reagent or carrier) ; Sucrose - chemistry ; T-Lymphocytes - chemistry ; T-Lymphocytes - physiology ; Water - chemistry</subject><ispartof>Biomacromolecules, 2007-09, Vol.8 (9), p.2815-2820</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a409t-e38e35428577ca219e3fe474da23898d4cdff5d6c0e17c6aee846781442132093</citedby><cites>FETCH-LOGICAL-a409t-e38e35428577ca219e3fe474da23898d4cdff5d6c0e17c6aee846781442132093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bm7005467$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bm7005467$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19070604$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17715963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mori, Toshiaki</creatorcontrib><creatorcontrib>Sekine, Yoshimi</creatorcontrib><creatorcontrib>Hasegawa, Mirei</creatorcontrib><creatorcontrib>Okahata, Yoshio</creatorcontrib><title>Nanometer-Scale Immobilization of Polysaccharides on Hydrophobic Polymer Plates in Supercritical Fluoroform/Water Emulsions</title><title>Biomacromolecules</title><addtitle>Biomacromolecules</addtitle><description>Hydrophilic polysaccharides such as dextran and hyaluronan were immobilized on a hydrophobic polystyrene (PSt) plate by a nanometer-scale surface penetration method in the emulsion of aqueous solutions in supercritical fluoroform (scCHF3). Since a supercritical fluid has high diffusiveness, water emulsions of polysaccharides can penetrate into the polymer surface. Dextran was surface-penetrated by two different methods: (1) the penetration of sucrose as a glucose donor and then the enzymatic polymerization to dextran near the surface catalyzed by dextransucrase, and (2) the direct penetration of dextran polymer into the PSt plate. The contact angle for water of the dextran-penetrated PSt plate was decreased to 78° from 95° of the untreated plate. The surface coverage and the penetration depth of polysaccharides could be obtained to be 10−30% and 10−20 nm, respectively, by X-ray photoelectron spectroscopy. These values could be controlled by the pressure of scCHF3. The transparency of the PSt dish did not change after the dextran penetration. Dextrans on the PSt plate could be elongated enzymatically by dextransucrase in the presence of sucrose as a glucose donor, and be detected by the enzyme-linked biotin−avidin assay. When anionic hyaluronan was surface-penetrated on the PSt plate instead of the neutral dextran, the plate showed the specific adhesion for human T-cells having hyaluronan receptors.</description><subject>Applied sciences</subject><subject>Cells, Immobilized</subject><subject>Chlorofluorocarbons - chemistry</subject><subject>Dextrans - chemistry</subject><subject>Emulsions - chemistry</subject><subject>Exact sciences and technology</subject><subject>Fluorocarbons - chemistry</subject><subject>Humans</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Nanostructures</subject><subject>Organic polymers</subject><subject>Physicochemistry of polymers</subject><subject>Polymers - chemistry</subject><subject>Polysaccharides - chemistry</subject><subject>Properties and characterization</subject><subject>Special properties (catalyst, reagent or carrier)</subject><subject>Sucrose - chemistry</subject><subject>T-Lymphocytes - chemistry</subject><subject>T-Lymphocytes - physiology</subject><subject>Water - chemistry</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1LAzEQhoMoflQP_gHZi4KHtUk22ewepVgtiBaqeFzS7CxNSTY12T1U_7yxLfbiaYaZh3eYB6FLgu8IpmQ4twJjznJxgE4Jp3nKckwPNz1PhSjFCToLYYkxLjPGj9EJEYLwMs9O0feLbJ2FDnw6U9JAMrHWzbXRX7LTrk1ck0ydWQep1EJ6XUNI4vRpXXu3WkRQbdYWfDI1sotb3SazfgVeed3pmJiMTe-8a5y3w49I-OTB9ibE7HCOjhppAlzs6gC9jx_eRk_p8-vjZHT_nEqGyy6FrICMM1pwIZSkpISsASZYLWlWlEXNVN00vM4VBiJULgGKqKIgjFGS0fjyAN1sc1feffYQusrqoMAY2YLrQ5UXlFPBSARvt6DyLgQPTbXy2kq_rgiufk1Xf6Yje7UL7ecW6j25UxuB6x0gQ_TQeNkqHfZciQXOMdtzUoVq6XrfRhf_HPwBmp-TKw</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Mori, Toshiaki</creator><creator>Sekine, Yoshimi</creator><creator>Hasegawa, Mirei</creator><creator>Okahata, Yoshio</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070901</creationdate><title>Nanometer-Scale Immobilization of Polysaccharides on Hydrophobic Polymer Plates in Supercritical Fluoroform/Water Emulsions</title><author>Mori, Toshiaki ; Sekine, Yoshimi ; Hasegawa, Mirei ; Okahata, Yoshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a409t-e38e35428577ca219e3fe474da23898d4cdff5d6c0e17c6aee846781442132093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Applied sciences</topic><topic>Cells, Immobilized</topic><topic>Chlorofluorocarbons - chemistry</topic><topic>Dextrans - chemistry</topic><topic>Emulsions - chemistry</topic><topic>Exact sciences and technology</topic><topic>Fluorocarbons - chemistry</topic><topic>Humans</topic><topic>Hyaluronic Acid - chemistry</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Nanostructures</topic><topic>Organic polymers</topic><topic>Physicochemistry of polymers</topic><topic>Polymers - chemistry</topic><topic>Polysaccharides - chemistry</topic><topic>Properties and characterization</topic><topic>Special properties (catalyst, reagent or carrier)</topic><topic>Sucrose - chemistry</topic><topic>T-Lymphocytes - chemistry</topic><topic>T-Lymphocytes - physiology</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mori, Toshiaki</creatorcontrib><creatorcontrib>Sekine, Yoshimi</creatorcontrib><creatorcontrib>Hasegawa, Mirei</creatorcontrib><creatorcontrib>Okahata, Yoshio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomacromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mori, Toshiaki</au><au>Sekine, Yoshimi</au><au>Hasegawa, Mirei</au><au>Okahata, Yoshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanometer-Scale Immobilization of Polysaccharides on Hydrophobic Polymer Plates in Supercritical Fluoroform/Water Emulsions</atitle><jtitle>Biomacromolecules</jtitle><addtitle>Biomacromolecules</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>8</volume><issue>9</issue><spage>2815</spage><epage>2820</epage><pages>2815-2820</pages><issn>1525-7797</issn><eissn>1526-4602</eissn><abstract>Hydrophilic polysaccharides such as dextran and hyaluronan were immobilized on a hydrophobic polystyrene (PSt) plate by a nanometer-scale surface penetration method in the emulsion of aqueous solutions in supercritical fluoroform (scCHF3). Since a supercritical fluid has high diffusiveness, water emulsions of polysaccharides can penetrate into the polymer surface. Dextran was surface-penetrated by two different methods: (1) the penetration of sucrose as a glucose donor and then the enzymatic polymerization to dextran near the surface catalyzed by dextransucrase, and (2) the direct penetration of dextran polymer into the PSt plate. The contact angle for water of the dextran-penetrated PSt plate was decreased to 78° from 95° of the untreated plate. The surface coverage and the penetration depth of polysaccharides could be obtained to be 10−30% and 10−20 nm, respectively, by X-ray photoelectron spectroscopy. These values could be controlled by the pressure of scCHF3. The transparency of the PSt dish did not change after the dextran penetration. Dextrans on the PSt plate could be elongated enzymatically by dextransucrase in the presence of sucrose as a glucose donor, and be detected by the enzyme-linked biotin−avidin assay. When anionic hyaluronan was surface-penetrated on the PSt plate instead of the neutral dextran, the plate showed the specific adhesion for human T-cells having hyaluronan receptors.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17715963</pmid><doi>10.1021/bm7005467</doi><tpages>6</tpages></addata></record> |
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subjects | Applied sciences Cells, Immobilized Chlorofluorocarbons - chemistry Dextrans - chemistry Emulsions - chemistry Exact sciences and technology Fluorocarbons - chemistry Humans Hyaluronic Acid - chemistry Hydrophobic and Hydrophilic Interactions Nanostructures Organic polymers Physicochemistry of polymers Polymers - chemistry Polysaccharides - chemistry Properties and characterization Special properties (catalyst, reagent or carrier) Sucrose - chemistry T-Lymphocytes - chemistry T-Lymphocytes - physiology Water - chemistry |
title | Nanometer-Scale Immobilization of Polysaccharides on Hydrophobic Polymer Plates in Supercritical Fluoroform/Water Emulsions |
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