Specific Integrin Labeling in Living Cells Using Functionalized Nanocrystals
We present an integrin labeling method using functionalized quantum dots (QDs). Cyclic Arg‐Gly‐Asp (RGD) peptides and a biotin–streptavidin linkage are used to specifically couple individual QDs to integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucia...
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| Veröffentlicht in: | Small (Weinheim an der Bergstrasse, Germany) Germany), 2007-09, Vol.3 (9), p.1560-1565 |
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| creator | Lieleg, Oliver López-García, Mónica Semmrich, Christine Auernheimer, Jörg Kessler, Horst Bausch, Andreas R. |
| description | We present an integrin labeling method using functionalized quantum dots (QDs). Cyclic Arg‐Gly‐Asp (RGD) peptides and a biotin–streptavidin linkage are used to specifically couple individual QDs to integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter to ensure specific binding to individual αvβ3 integrins of osteoblast cells. Despite blinking, the position of single QDs is tracked with nanometer precision and localized diffusive behavior is observed. We show that blinking events do not prevent the acquisition of quantitative parameters from the QD trajectories.
Twinkle, twinkle, little dot: Quantum dots (QDs) functionalized by cyclic Arg‐Gly‐Asp (cRGD) peptides using a biotin–streptavidin linkage can specifically label integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter that ensures specific binding to individual αvβ3 integrins of osteoblast cells (see picture). Despite blinking, the position of single QDs is tracked with nanometer precision. |
| doi_str_mv | 10.1002/smll.200700148 |
| format | Article |
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Twinkle, twinkle, little dot: Quantum dots (QDs) functionalized by cyclic Arg‐Gly‐Asp (cRGD) peptides using a biotin–streptavidin linkage can specifically label integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter that ensures specific binding to individual αvβ3 integrins of osteoblast cells (see picture). Despite blinking, the position of single QDs is tracked with nanometer precision.</description><identifier>ISSN: 1613-6810</identifier><identifier>EISSN: 1613-6829</identifier><identifier>DOI: 10.1002/smll.200700148</identifier><identifier>PMID: 17705315</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Animals ; bioimaging ; cells ; Cells, Cultured ; Crystallization - methods ; Integrin alphaVbeta3 - chemistry ; Integrin alphaVbeta3 - metabolism ; labeling ; Macromolecular Substances - chemistry ; Materials Testing ; Microscopy, Fluorescence - methods ; Molecular Conformation ; Nanotechnology - methods ; Oligopeptides - chemistry ; Oligopeptides - metabolism ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Particle Size ; peptides ; Quantum Dots ; Rats ; Spectrometry, Fluorescence - methods ; Staining and Labeling - methods ; Surface Properties</subject><ispartof>Small (Weinheim an der Bergstrasse, Germany), 2007-09, Vol.3 (9), p.1560-1565</ispartof><rights>Copyright © 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4128-a36b7d6433b6a864bee1e2f46a7fe61c2fdfe54417a902ee0cfa2f5f4a024fc63</citedby><cites>FETCH-LOGICAL-c4128-a36b7d6433b6a864bee1e2f46a7fe61c2fdfe54417a902ee0cfa2f5f4a024fc63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fsmll.200700148$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fsmll.200700148$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17705315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lieleg, Oliver</creatorcontrib><creatorcontrib>López-García, Mónica</creatorcontrib><creatorcontrib>Semmrich, Christine</creatorcontrib><creatorcontrib>Auernheimer, Jörg</creatorcontrib><creatorcontrib>Kessler, Horst</creatorcontrib><creatorcontrib>Bausch, Andreas R.</creatorcontrib><title>Specific Integrin Labeling in Living Cells Using Functionalized Nanocrystals</title><title>Small (Weinheim an der Bergstrasse, Germany)</title><addtitle>Small</addtitle><description>We present an integrin labeling method using functionalized quantum dots (QDs). Cyclic Arg‐Gly‐Asp (RGD) peptides and a biotin–streptavidin linkage are used to specifically couple individual QDs to integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter to ensure specific binding to individual αvβ3 integrins of osteoblast cells. Despite blinking, the position of single QDs is tracked with nanometer precision and localized diffusive behavior is observed. We show that blinking events do not prevent the acquisition of quantitative parameters from the QD trajectories.
Twinkle, twinkle, little dot: Quantum dots (QDs) functionalized by cyclic Arg‐Gly‐Asp (cRGD) peptides using a biotin–streptavidin linkage can specifically label integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter that ensures specific binding to individual αvβ3 integrins of osteoblast cells (see picture). Despite blinking, the position of single QDs is tracked with nanometer precision.</description><subject>Animals</subject><subject>bioimaging</subject><subject>cells</subject><subject>Cells, Cultured</subject><subject>Crystallization - methods</subject><subject>Integrin alphaVbeta3 - chemistry</subject><subject>Integrin alphaVbeta3 - metabolism</subject><subject>labeling</subject><subject>Macromolecular Substances - chemistry</subject><subject>Materials Testing</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Molecular Conformation</subject><subject>Nanotechnology - methods</subject><subject>Oligopeptides - chemistry</subject><subject>Oligopeptides - metabolism</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Particle Size</subject><subject>peptides</subject><subject>Quantum Dots</subject><subject>Rats</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Staining and Labeling - methods</subject><subject>Surface Properties</subject><issn>1613-6810</issn><issn>1613-6829</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPwzAQhC0E4n3liHLilrJ-xE6OqKKAFF4qCImL5bhrZEiTEqdA-fUkalW4cZo5fDPaHUKOKAwoADsN07IcMAAFQEW6QXappDyWKcs2157CDtkL4RWAUybUNtmhSkHCabJL8vEMrXfeRldViy-Nr6LcFFj66iXqvf_o3RDLMkSPofejeWVbX1em9N84iW5MVdtmEVpThgOy5TrBw5Xuk8fR-cPwMs5vL66GZ3lsBWVpbLgs1EQKzgtpUikKRIrMCWmUQ0ktcxOHiRBUmQwYIlhnmEucMMCEs5Lvk5Nl76yp3-cYWj31wXY3mgrredDd-wkT8n-QA2SUpmkHDpagbeoQGnR61vipaRaagu6H1v3Qej10FzheNc-LKU5-8dWyHZAtgU9f4uKfOj2-zvO_5fEy60OLX-usad60VFwl-unmQjOeqfHzHdP3_AeBzJn4</recordid><startdate>20070903</startdate><enddate>20070903</enddate><creator>Lieleg, Oliver</creator><creator>López-García, Mónica</creator><creator>Semmrich, Christine</creator><creator>Auernheimer, Jörg</creator><creator>Kessler, Horst</creator><creator>Bausch, Andreas R.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20070903</creationdate><title>Specific Integrin Labeling in Living Cells Using Functionalized Nanocrystals</title><author>Lieleg, Oliver ; López-García, Mónica ; Semmrich, Christine ; Auernheimer, Jörg ; Kessler, Horst ; Bausch, Andreas R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4128-a36b7d6433b6a864bee1e2f46a7fe61c2fdfe54417a902ee0cfa2f5f4a024fc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>bioimaging</topic><topic>cells</topic><topic>Cells, Cultured</topic><topic>Crystallization - methods</topic><topic>Integrin alphaVbeta3 - chemistry</topic><topic>Integrin alphaVbeta3 - metabolism</topic><topic>labeling</topic><topic>Macromolecular Substances - chemistry</topic><topic>Materials Testing</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Molecular Conformation</topic><topic>Nanotechnology - methods</topic><topic>Oligopeptides - chemistry</topic><topic>Oligopeptides - metabolism</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Particle Size</topic><topic>peptides</topic><topic>Quantum Dots</topic><topic>Rats</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Staining and Labeling - methods</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lieleg, Oliver</creatorcontrib><creatorcontrib>López-García, Mónica</creatorcontrib><creatorcontrib>Semmrich, Christine</creatorcontrib><creatorcontrib>Auernheimer, Jörg</creatorcontrib><creatorcontrib>Kessler, Horst</creatorcontrib><creatorcontrib>Bausch, Andreas R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Small (Weinheim an der Bergstrasse, Germany)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lieleg, Oliver</au><au>López-García, Mónica</au><au>Semmrich, Christine</au><au>Auernheimer, Jörg</au><au>Kessler, Horst</au><au>Bausch, Andreas R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific Integrin Labeling in Living Cells Using Functionalized Nanocrystals</atitle><jtitle>Small (Weinheim an der Bergstrasse, Germany)</jtitle><addtitle>Small</addtitle><date>2007-09-03</date><risdate>2007</risdate><volume>3</volume><issue>9</issue><spage>1560</spage><epage>1565</epage><pages>1560-1565</pages><issn>1613-6810</issn><eissn>1613-6829</eissn><abstract>We present an integrin labeling method using functionalized quantum dots (QDs). Cyclic Arg‐Gly‐Asp (RGD) peptides and a biotin–streptavidin linkage are used to specifically couple individual QDs to integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter to ensure specific binding to individual αvβ3 integrins of osteoblast cells. Despite blinking, the position of single QDs is tracked with nanometer precision and localized diffusive behavior is observed. We show that blinking events do not prevent the acquisition of quantitative parameters from the QD trajectories.
Twinkle, twinkle, little dot: Quantum dots (QDs) functionalized by cyclic Arg‐Gly‐Asp (cRGD) peptides using a biotin–streptavidin linkage can specifically label integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter that ensures specific binding to individual αvβ3 integrins of osteoblast cells (see picture). Despite blinking, the position of single QDs is tracked with nanometer precision.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>17705315</pmid><doi>10.1002/smll.200700148</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals bioimaging cells Cells, Cultured Crystallization - methods Integrin alphaVbeta3 - chemistry Integrin alphaVbeta3 - metabolism labeling Macromolecular Substances - chemistry Materials Testing Microscopy, Fluorescence - methods Molecular Conformation Nanotechnology - methods Oligopeptides - chemistry Oligopeptides - metabolism Osteoblasts - cytology Osteoblasts - metabolism Particle Size peptides Quantum Dots Rats Spectrometry, Fluorescence - methods Staining and Labeling - methods Surface Properties |
| title | Specific Integrin Labeling in Living Cells Using Functionalized Nanocrystals |
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