An imbalance in antioxidant enzymes and stress proteins in childhood asthma
The study was undertaken to examine antioxidant status and level of the major intracellular heat shock proteins (Hsps) in healthy children and children with mild and moderate asthma. Native gel assays were performed to estimate activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase...
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| Veröffentlicht in: | Clinical biochemistry 2007-10, Vol.40 (15), p.1168-1171 |
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| creator | Perišić, Tatjana Srećković, Miodrag Matić, Gordana |
| description | The study was undertaken to examine antioxidant status and level of the major intracellular heat shock proteins (Hsps) in healthy children and children with mild and moderate asthma.
Native gel assays were performed to estimate activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase (SOD), and catalase (CAT) in peripheral blood mononuclear cells (PBMC) of healthy and asthmatic children. Hsp70 and Hsp90 protein levels in PBMCs were assessed by Western blot analysis.
Moderate asthmatics displayed higher CuZnSOD/CAT activity ratio compared to healthy children, and increased Hsp90 level compared to mild asthmatics and healthy children.
With regard to the imbalance in the antioxidant enzyme activities children with moderate asthma differ from healthy children, while an increased Hsp90 expression could be associated with the disease severity, as well. |
| doi_str_mv | 10.1016/j.clinbiochem.2007.06.006 |
| format | Article |
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Native gel assays were performed to estimate activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase (SOD), and catalase (CAT) in peripheral blood mononuclear cells (PBMC) of healthy and asthmatic children. Hsp70 and Hsp90 protein levels in PBMCs were assessed by Western blot analysis.
Moderate asthmatics displayed higher CuZnSOD/CAT activity ratio compared to healthy children, and increased Hsp90 level compared to mild asthmatics and healthy children.
With regard to the imbalance in the antioxidant enzyme activities children with moderate asthma differ from healthy children, while an increased Hsp90 expression could be associated with the disease severity, as well.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/j.clinbiochem.2007.06.006</identifier><identifier>PMID: 17689515</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Antioxidants - analysis ; Asthma ; Asthma - blood ; Asthma - enzymology ; Case-Control Studies ; Catalase ; Catalase - blood ; Child ; Children ; Heat shock proteins ; Heat-Shock Proteins - blood ; HSP70 Heat-Shock Proteins - analysis ; HSP70 Heat-Shock Proteins - blood ; HSP90 Heat-Shock Proteins - analysis ; HSP90 Heat-Shock Proteins - blood ; Humans ; Leukocytes, Mononuclear - chemistry ; Leukocytes, Mononuclear - enzymology ; Superoxide dismutase ; Superoxide Dismutase - blood</subject><ispartof>Clinical biochemistry, 2007-10, Vol.40 (15), p.1168-1171</ispartof><rights>2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-3446187a716f6aa8ad2c5f764ea0213c913b6788f4b27dd8ac0506f10f60251e3</citedby><cites>FETCH-LOGICAL-c375t-3446187a716f6aa8ad2c5f764ea0213c913b6788f4b27dd8ac0506f10f60251e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.clinbiochem.2007.06.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17689515$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Perišić, Tatjana</creatorcontrib><creatorcontrib>Srećković, Miodrag</creatorcontrib><creatorcontrib>Matić, Gordana</creatorcontrib><title>An imbalance in antioxidant enzymes and stress proteins in childhood asthma</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>The study was undertaken to examine antioxidant status and level of the major intracellular heat shock proteins (Hsps) in healthy children and children with mild and moderate asthma.
Native gel assays were performed to estimate activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase (SOD), and catalase (CAT) in peripheral blood mononuclear cells (PBMC) of healthy and asthmatic children. Hsp70 and Hsp90 protein levels in PBMCs were assessed by Western blot analysis.
Moderate asthmatics displayed higher CuZnSOD/CAT activity ratio compared to healthy children, and increased Hsp90 level compared to mild asthmatics and healthy children.
With regard to the imbalance in the antioxidant enzyme activities children with moderate asthma differ from healthy children, while an increased Hsp90 expression could be associated with the disease severity, as well.</description><subject>Adolescent</subject><subject>Antioxidants - analysis</subject><subject>Asthma</subject><subject>Asthma - blood</subject><subject>Asthma - enzymology</subject><subject>Case-Control Studies</subject><subject>Catalase</subject><subject>Catalase - blood</subject><subject>Child</subject><subject>Children</subject><subject>Heat shock proteins</subject><subject>Heat-Shock Proteins - blood</subject><subject>HSP70 Heat-Shock Proteins - analysis</subject><subject>HSP70 Heat-Shock Proteins - blood</subject><subject>HSP90 Heat-Shock Proteins - analysis</subject><subject>HSP90 Heat-Shock Proteins - blood</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - chemistry</subject><subject>Leukocytes, Mononuclear - enzymology</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - blood</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1PwzAMhiMEYmPwF1C5cGtxmjZJj9PEl5jEBc5RmrhaprYZTYcYv55MmwRHTpajx7Hfh5AbChkFyu_WmWldXztvVthlOYDIgGcA_IRMqRQszSvGTskUAKq0ojlMyEUI69jmheTnZEIFl1VJyyl5mfeJ62rd6t5g4vpE96PzX87GmmD_veswxDebhHHAEJLN4Ed0fdijZuVau_LeJjqMq05fkrNGtwGvjnVG3h_u3xZP6fL18XkxX6aGiXJMWVHweKUWlDdca6ltbspG8AI15JSZirKaCymbos6FtVIbKIE3FBoOeUmRzcjt4d94zMcWw6g6Fwy2MQP6bVBcxpjAZQSrA2gGH8KAjdoMrtPDTlFQe5Nqrf6YVHuTCriKJuPs9XHJtu7Q_k4e1UVgcQAwRv10OKhgHEaL1g1oRmW9-8eaHzBCirE</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Perišić, Tatjana</creator><creator>Srećković, Miodrag</creator><creator>Matić, Gordana</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>An imbalance in antioxidant enzymes and stress proteins in childhood asthma</title><author>Perišić, Tatjana ; Srećković, Miodrag ; Matić, Gordana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-3446187a716f6aa8ad2c5f764ea0213c913b6788f4b27dd8ac0506f10f60251e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adolescent</topic><topic>Antioxidants - analysis</topic><topic>Asthma</topic><topic>Asthma - blood</topic><topic>Asthma - enzymology</topic><topic>Case-Control Studies</topic><topic>Catalase</topic><topic>Catalase - blood</topic><topic>Child</topic><topic>Children</topic><topic>Heat shock proteins</topic><topic>Heat-Shock Proteins - blood</topic><topic>HSP70 Heat-Shock Proteins - analysis</topic><topic>HSP70 Heat-Shock Proteins - blood</topic><topic>HSP90 Heat-Shock Proteins - analysis</topic><topic>HSP90 Heat-Shock Proteins - blood</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - chemistry</topic><topic>Leukocytes, Mononuclear - enzymology</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Perišić, Tatjana</creatorcontrib><creatorcontrib>Srećković, Miodrag</creatorcontrib><creatorcontrib>Matić, Gordana</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perišić, Tatjana</au><au>Srećković, Miodrag</au><au>Matić, Gordana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An imbalance in antioxidant enzymes and stress proteins in childhood asthma</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>40</volume><issue>15</issue><spage>1168</spage><epage>1171</epage><pages>1168-1171</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>The study was undertaken to examine antioxidant status and level of the major intracellular heat shock proteins (Hsps) in healthy children and children with mild and moderate asthma.
Native gel assays were performed to estimate activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase (SOD), and catalase (CAT) in peripheral blood mononuclear cells (PBMC) of healthy and asthmatic children. Hsp70 and Hsp90 protein levels in PBMCs were assessed by Western blot analysis.
Moderate asthmatics displayed higher CuZnSOD/CAT activity ratio compared to healthy children, and increased Hsp90 level compared to mild asthmatics and healthy children.
With regard to the imbalance in the antioxidant enzyme activities children with moderate asthma differ from healthy children, while an increased Hsp90 expression could be associated with the disease severity, as well.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17689515</pmid><doi>10.1016/j.clinbiochem.2007.06.006</doi><tpages>4</tpages></addata></record> |
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| ispartof | Clinical biochemistry, 2007-10, Vol.40 (15), p.1168-1171 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adolescent Antioxidants - analysis Asthma Asthma - blood Asthma - enzymology Case-Control Studies Catalase Catalase - blood Child Children Heat shock proteins Heat-Shock Proteins - blood HSP70 Heat-Shock Proteins - analysis HSP70 Heat-Shock Proteins - blood HSP90 Heat-Shock Proteins - analysis HSP90 Heat-Shock Proteins - blood Humans Leukocytes, Mononuclear - chemistry Leukocytes, Mononuclear - enzymology Superoxide dismutase Superoxide Dismutase - blood |
| title | An imbalance in antioxidant enzymes and stress proteins in childhood asthma |
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