Extending Jak2V617F and MplW515 Mutation Analysis to Single Hematopoietic Colonies and B and T Lymphocytes

JAK2V617F and MPLW515L/K are myeloproliferative disorder (MPD)‐associated mutations. We genotyped 552 individual hematopoietic colonies obtained by CD34+ cell culture from 16 affected patients (13 JAK2V617F and 3 MPLW515L/K) to determine (a) the proportion of colonies harboring a particular mutation...

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Veröffentlicht in:	Stem cells (Dayton, Ohio) Ohio), 2007-09, Vol.25 (9), p.2358-2362
Hauptverfasser:	Pardanani, Animesh, Lasho, Terra L., Finke, Christy, Mesa, Ruben A., Hogan, William J., Ketterling, Rhett P., Gilliland, Dwight Gary, Tefferi, Ayalew
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution B-Lymphocytes - cytology B-Lymphocytes - metabolism Base Sequence Cells, Cultured Clone Cells - metabolism Colony formation DNA Mutational Analysis Hematopoiesis - genetics Hematopoietic progenitor cells Human CD34 and CD43 cells Humans JAK kinase Janus Kinase 2 - genetics Janus Kinase 2 - metabolism Myelopoiesis Point Mutation Polycythemia Vera - genetics Primary Myelofibrosis - genetics Receptors, Thrombopoietin - genetics Receptors, Thrombopoietin - metabolism T-Lymphocytes - cytology T-Lymphocytes - metabolism Tryptophan - genetics Valine - genetics
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