Neutrophils in induced sputum from healthy children: Role of interleukin-8 and oxidative stress
Summary Background It is unclear why the neutrophil differential count in induced sputum (IS) from normal children is highly variable. Since levels of neutrophil chemoattractant cytokines and oxidative stress are determinants of airway neutrophilia in animal models, we sought to determine the associ...
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| Veröffentlicht in: | Respiratory medicine 2007-10, Vol.101 (10), p.2108-2112 |
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| description | Summary Background It is unclear why the neutrophil differential count in induced sputum (IS) from normal children is highly variable. Since levels of neutrophil chemoattractant cytokines and oxidative stress are determinants of airway neutrophilia in animal models, we sought to determine the association between IS neutrophils from healthy children and (i) interleukin-8 (IL-8) and (ii) the oxidative stress marker 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG). Methods IS was done using hypertonic saline. The proportion (differential %) and the absolute number of IS neutrophils were determined by light microscopy. IS IL-8 and 8-oxodG were determined using ELISA. Spearman's rank correlation (Rs) was used to assess relationship between variables. Results Adequate IS samples for analysis were obtained from 64/114 healthy children. The median (interquartile range) neutrophil differential count ( n =64) was 20.6% (5.67–56), and absolute neutrophil count ( n =53) was 0.11×106 (0.01–0.77). Both the % neutrophils, and the absolute neutrophil count were associated with IL-8 (Rs=0.67, p |
| doi_str_mv | 10.1016/j.rmed.2007.05.018 |
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Since levels of neutrophil chemoattractant cytokines and oxidative stress are determinants of airway neutrophilia in animal models, we sought to determine the association between IS neutrophils from healthy children and (i) interleukin-8 (IL-8) and (ii) the oxidative stress marker 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG). Methods IS was done using hypertonic saline. The proportion (differential %) and the absolute number of IS neutrophils were determined by light microscopy. IS IL-8 and 8-oxodG were determined using ELISA. Spearman's rank correlation (Rs) was used to assess relationship between variables. Results Adequate IS samples for analysis were obtained from 64/114 healthy children. The median (interquartile range) neutrophil differential count ( n =64) was 20.6% (5.67–56), and absolute neutrophil count ( n =53) was 0.11×106 (0.01–0.77). Both the % neutrophils, and the absolute neutrophil count were associated with IL-8 (Rs=0.67, p <0.0001 and 0.60, p <0.0001, respectively), but there was no association between IS neutrophil variables and 8-oxodG ( n =40). The repeatability (intraclass correlation coefficient—Ri) of the neutrophil differential count was 0.58 ( n =15). Conclusions The variation in the proportion and number of neutrophils in the lower airway of healthy children is associated with IL-8, but not with oxidative stress. The IS neutrophil differential count in healthy children is relatively stable over several months.</description><identifier>ISSN: 0954-6111</identifier><identifier>EISSN: 1532-3064</identifier><identifier>DOI: 10.1016/j.rmed.2007.05.018</identifier><identifier>PMID: 17618102</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adolescent ; Biological and medical sciences ; Child ; Female ; Healthy children ; Humans ; Induced sputum ; Interleukin-8 ; Interleukin-8 - analysis ; Interleukin-8 - immunology ; Leukocyte Count ; Male ; Medical sciences ; Neutrophil differential count ; Neutrophils - immunology ; Oxidant stress ; Oxidative Stress - immunology ; Pneumology ; Pulmonary/Respiratory ; Sputum - cytology ; Sputum - immunology</subject><ispartof>Respiratory medicine, 2007-10, Vol.101 (10), p.2108-2112</ispartof><rights>Elsevier Ltd</rights><rights>2007 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-72f7a651d2df777fd6302e36421c2fc5cfa13343139d0b088ceb772863726513</citedby><cites>FETCH-LOGICAL-c577t-72f7a651d2df777fd6302e36421c2fc5cfa13343139d0b088ceb772863726513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rmed.2007.05.018$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19085463$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17618102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kulkarni, Neeta</creatorcontrib><creatorcontrib>Cooke, Marcus S</creatorcontrib><creatorcontrib>Grigg, Jonathan</creatorcontrib><title>Neutrophils in induced sputum from healthy children: Role of interleukin-8 and oxidative stress</title><title>Respiratory medicine</title><addtitle>Respir Med</addtitle><description>Summary Background It is unclear why the neutrophil differential count in induced sputum (IS) from normal children is highly variable. Since levels of neutrophil chemoattractant cytokines and oxidative stress are determinants of airway neutrophilia in animal models, we sought to determine the association between IS neutrophils from healthy children and (i) interleukin-8 (IL-8) and (ii) the oxidative stress marker 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG). Methods IS was done using hypertonic saline. The proportion (differential %) and the absolute number of IS neutrophils were determined by light microscopy. IS IL-8 and 8-oxodG were determined using ELISA. Spearman's rank correlation (Rs) was used to assess relationship between variables. Results Adequate IS samples for analysis were obtained from 64/114 healthy children. The median (interquartile range) neutrophil differential count ( n =64) was 20.6% (5.67–56), and absolute neutrophil count ( n =53) was 0.11×106 (0.01–0.77). Both the % neutrophils, and the absolute neutrophil count were associated with IL-8 (Rs=0.67, p <0.0001 and 0.60, p <0.0001, respectively), but there was no association between IS neutrophil variables and 8-oxodG ( n =40). The repeatability (intraclass correlation coefficient—Ri) of the neutrophil differential count was 0.58 ( n =15). Conclusions The variation in the proportion and number of neutrophils in the lower airway of healthy children is associated with IL-8, but not with oxidative stress. The IS neutrophil differential count in healthy children is relatively stable over several months.</description><subject>Adolescent</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Female</subject><subject>Healthy children</subject><subject>Humans</subject><subject>Induced sputum</subject><subject>Interleukin-8</subject><subject>Interleukin-8 - analysis</subject><subject>Interleukin-8 - immunology</subject><subject>Leukocyte Count</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neutrophil differential count</subject><subject>Neutrophils - immunology</subject><subject>Oxidant stress</subject><subject>Oxidative Stress - immunology</subject><subject>Pneumology</subject><subject>Pulmonary/Respiratory</subject><subject>Sputum - cytology</subject><subject>Sputum - immunology</subject><issn>0954-6111</issn><issn>1532-3064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk-LFDEQxYMo7jj6BTxIg-it20rSSXpkEWTxHywKuveQSSpMZrs7Y9K9ON_eNDO4sAehIIf8XuXVSxHykkJDgcp3-yYN6BoGoBoQDdDuEVlRwVnNQbaPyQo2oq0lpfSCPMt5DwCbtoWn5IIqSTsKbEX0d5ynFA-70OcqjKXcbNFV-TBP81D5FIdqh6afdsfKFsglHN9XP2OPVfSFnjD1ON-Gse4qM7oq_gnOTOEOqzwlzPk5eeJNn_HF-VyTm8-fbq6-1tc_vny7-nhdW6HUVCvmlZGCOua8Uso7yYEhly2jlnkrrDeU85ZTvnGwha6zuFWKdZIrVmR8Td6e2h5S_D1jnvQQssW-NyPGOWvZsVYxwQr4-gG4j3MaizVNgQtoBS-1JuxE2RRzTuj1IYXBpGOB9JK93usle71kr0Hokn0RvTq3nrfL3T_JOewCvDkDJlvT-2RGG_I9t4FOtJIX7vLEYQnsLmDS2QYcy7eEhHbSLob_-_jwQG77MIby4i0eMd_PqzPToH8tW7IsCSgoJkv9BeeZtdw</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Kulkarni, Neeta</creator><creator>Cooke, Marcus S</creator><creator>Grigg, Jonathan</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>ASE</scope><scope>FPQ</scope><scope>H94</scope><scope>K6X</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>Neutrophils in induced sputum from healthy children: Role of interleukin-8 and oxidative stress</title><author>Kulkarni, Neeta ; Cooke, Marcus S ; Grigg, Jonathan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-72f7a651d2df777fd6302e36421c2fc5cfa13343139d0b088ceb772863726513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adolescent</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Female</topic><topic>Healthy children</topic><topic>Humans</topic><topic>Induced sputum</topic><topic>Interleukin-8</topic><topic>Interleukin-8 - analysis</topic><topic>Interleukin-8 - immunology</topic><topic>Leukocyte Count</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neutrophil differential count</topic><topic>Neutrophils - immunology</topic><topic>Oxidant stress</topic><topic>Oxidative Stress - immunology</topic><topic>Pneumology</topic><topic>Pulmonary/Respiratory</topic><topic>Sputum - cytology</topic><topic>Sputum - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kulkarni, Neeta</creatorcontrib><creatorcontrib>Cooke, Marcus S</creatorcontrib><creatorcontrib>Grigg, Jonathan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>British Nursing Index</collection><collection>British Nursing Index (BNI) (1985 to Present)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>British Nursing Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Respiratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kulkarni, Neeta</au><au>Cooke, Marcus S</au><au>Grigg, Jonathan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutrophils in induced sputum from healthy children: Role of interleukin-8 and oxidative stress</atitle><jtitle>Respiratory medicine</jtitle><addtitle>Respir Med</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>101</volume><issue>10</issue><spage>2108</spage><epage>2112</epage><pages>2108-2112</pages><issn>0954-6111</issn><eissn>1532-3064</eissn><abstract>Summary Background It is unclear why the neutrophil differential count in induced sputum (IS) from normal children is highly variable. Since levels of neutrophil chemoattractant cytokines and oxidative stress are determinants of airway neutrophilia in animal models, we sought to determine the association between IS neutrophils from healthy children and (i) interleukin-8 (IL-8) and (ii) the oxidative stress marker 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG). Methods IS was done using hypertonic saline. The proportion (differential %) and the absolute number of IS neutrophils were determined by light microscopy. IS IL-8 and 8-oxodG were determined using ELISA. Spearman's rank correlation (Rs) was used to assess relationship between variables. Results Adequate IS samples for analysis were obtained from 64/114 healthy children. The median (interquartile range) neutrophil differential count ( n =64) was 20.6% (5.67–56), and absolute neutrophil count ( n =53) was 0.11×106 (0.01–0.77). Both the % neutrophils, and the absolute neutrophil count were associated with IL-8 (Rs=0.67, p <0.0001 and 0.60, p <0.0001, respectively), but there was no association between IS neutrophil variables and 8-oxodG ( n =40). The repeatability (intraclass correlation coefficient—Ri) of the neutrophil differential count was 0.58 ( n =15). Conclusions The variation in the proportion and number of neutrophils in the lower airway of healthy children is associated with IL-8, but not with oxidative stress. The IS neutrophil differential count in healthy children is relatively stable over several months.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>17618102</pmid><doi>10.1016/j.rmed.2007.05.018</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Biological and medical sciences Child Female Healthy children Humans Induced sputum Interleukin-8 Interleukin-8 - analysis Interleukin-8 - immunology Leukocyte Count Male Medical sciences Neutrophil differential count Neutrophils - immunology Oxidant stress Oxidative Stress - immunology Pneumology Pulmonary/Respiratory Sputum - cytology Sputum - immunology |
| title | Neutrophils in induced sputum from healthy children: Role of interleukin-8 and oxidative stress |
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