Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice
Our previous studies have shown that the combined administration of drugs elevating extracellular adenosine, i.e. dipyridamole (DP) and adenosine monophosphate (AMP), enhances murine hematopoiesis and potentiates the action of granulocyte colony-stimulating factor (G-CSF). In this study, colony-stim...
Gespeichert in:
| Veröffentlicht in: | Physiological research 2007-01, Vol.56 (4), p.463-474 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 474 |
|---|---|
| container_issue | 4 |
| container_start_page | 463 |
| container_title | Physiological research |
| container_volume | 56 |
| creator | Weiterová, L Hofer, M Pospísil, M Znojil, V Streitová, D |
| description | Our previous studies have shown that the combined administration of drugs elevating extracellular adenosine, i.e. dipyridamole (DP) and adenosine monophosphate (AMP), enhances murine hematopoiesis and potentiates the action of granulocyte colony-stimulating factor (G-CSF). In this study, colony-stimulating activity (CSA) of blood serum of mice treated with DP+AMP, G-CSF or all these drugs in combination, i.e. the ability of the sera to stimulate the growth of GM-CFC colonies, was assayed in vitro. Furthermore, the concentration of GM-CSF and IL-6 in the sera was determined. Administration of DP+AMP was found to enhance significantly serum CSA at all time intervals of serum sampling including 24 h after the last injection of the tested drugs. Additive effects of DP+AMP and G-CSF on serum CSA were noted at early intervals after administration of the drugs. Furthermore, IL-6 levels were significantly elevated in the sera of mice which were administered DP+AMP either alone or in combination with G-CSF. Our results show that the effects of DP+AMP are indirect, mediated through the induction of some cytokine(s) and/or growth factor(s) and that extracellular adenosine can act in cooperation with G-CSF. These findings contribute to the further elucidation of the role of adenosine in hematopoiesis. |
| doi_str_mv | 10.33549/physiolres.931019 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68246403</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68246403</sourcerecordid><originalsourceid>FETCH-LOGICAL-c323t-b59d5242517c768b30aeaef8ae1b0cfbc3270ee4ad24bd8a045f24840d002af63</originalsourceid><addsrcrecordid>eNpdkTtPwzAUhS0EouXxBxhQxMCW4lccZ0S8JSQWmCPHuQGXxC52XNGJv45LK1Viuh7O98lHB6EzgmeMFby6WnysgnG9hzCrGMGk2kNTIjHNq6pk-2iKpaC55FhO0FEIc4xpiUt2iCZEVLTgQkzRz62P7yGDHpZqNPY9g-_RKw19H3vlM9WCdcFYSK_BWBNG8NBmxmZLs3TptlFDFsDHIdOud3aVh9EMif2TKT2apRlXmbJrKME9xE9jc7FWDEbDCTroVB_gdHuP0dv93evNY_788vB0c_2ca0bZmDdF1RaU04KUuhSyYViBgk4qIA3WXZNSJQbgqqW8aaXCvOgoT9XbVFp1gh2jy4134d1XhDDWgwnrmsqCi6EWknLBMUvBi3_BuYvepr_VlFDKJMFrG92EtHcheOjqhTeD8qua4Ppvm3q3Tb3ZJkHnW3NsBmh3yHYM9gt_JZDJ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>212238106</pqid></control><display><type>article</type><title>Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Weiterová, L ; Hofer, M ; Pospísil, M ; Znojil, V ; Streitová, D</creator><creatorcontrib>Weiterová, L ; Hofer, M ; Pospísil, M ; Znojil, V ; Streitová, D</creatorcontrib><description>Our previous studies have shown that the combined administration of drugs elevating extracellular adenosine, i.e. dipyridamole (DP) and adenosine monophosphate (AMP), enhances murine hematopoiesis and potentiates the action of granulocyte colony-stimulating factor (G-CSF). In this study, colony-stimulating activity (CSA) of blood serum of mice treated with DP+AMP, G-CSF or all these drugs in combination, i.e. the ability of the sera to stimulate the growth of GM-CFC colonies, was assayed in vitro. Furthermore, the concentration of GM-CSF and IL-6 in the sera was determined. Administration of DP+AMP was found to enhance significantly serum CSA at all time intervals of serum sampling including 24 h after the last injection of the tested drugs. Additive effects of DP+AMP and G-CSF on serum CSA were noted at early intervals after administration of the drugs. Furthermore, IL-6 levels were significantly elevated in the sera of mice which were administered DP+AMP either alone or in combination with G-CSF. Our results show that the effects of DP+AMP are indirect, mediated through the induction of some cytokine(s) and/or growth factor(s) and that extracellular adenosine can act in cooperation with G-CSF. These findings contribute to the further elucidation of the role of adenosine in hematopoiesis.</description><identifier>ISSN: 0862-8408</identifier><identifier>EISSN: 1802-9973</identifier><identifier>DOI: 10.33549/physiolres.931019</identifier><identifier>PMID: 16925466</identifier><language>eng</language><publisher>Czech Republic: Institute of Physiology</publisher><subject>Adenosine - metabolism ; Adenosine Monophosphate - metabolism ; Adenosine Monophosphate - pharmacology ; Animals ; Bone marrow ; Cells ; Colony-Forming Units Assay ; Dipyridamole - pharmacology ; Female ; Granulocyte Colony-Stimulating Factor - metabolism ; Granulocyte Colony-Stimulating Factor - pharmacology ; Granulocyte-Macrophage Colony-Stimulating Factor - blood ; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism ; Hematopoiesis - drug effects ; Hematopoietic Stem Cells - drug effects ; Hematopoietic Stem Cells - metabolism ; Interleukin-6 - blood ; Interleukin-6 - metabolism ; Kinases ; Male ; Mice ; Mice, Inbred ICR ; Proteins ; Rodents ; Time Factors ; Up-Regulation</subject><ispartof>Physiological research, 2007-01, Vol.56 (4), p.463-474</ispartof><rights>Copyright Institute of Physiology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16925466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weiterová, L</creatorcontrib><creatorcontrib>Hofer, M</creatorcontrib><creatorcontrib>Pospísil, M</creatorcontrib><creatorcontrib>Znojil, V</creatorcontrib><creatorcontrib>Streitová, D</creatorcontrib><title>Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice</title><title>Physiological research</title><addtitle>Physiol Res</addtitle><description>Our previous studies have shown that the combined administration of drugs elevating extracellular adenosine, i.e. dipyridamole (DP) and adenosine monophosphate (AMP), enhances murine hematopoiesis and potentiates the action of granulocyte colony-stimulating factor (G-CSF). In this study, colony-stimulating activity (CSA) of blood serum of mice treated with DP+AMP, G-CSF or all these drugs in combination, i.e. the ability of the sera to stimulate the growth of GM-CFC colonies, was assayed in vitro. Furthermore, the concentration of GM-CSF and IL-6 in the sera was determined. Administration of DP+AMP was found to enhance significantly serum CSA at all time intervals of serum sampling including 24 h after the last injection of the tested drugs. Additive effects of DP+AMP and G-CSF on serum CSA were noted at early intervals after administration of the drugs. Furthermore, IL-6 levels were significantly elevated in the sera of mice which were administered DP+AMP either alone or in combination with G-CSF. Our results show that the effects of DP+AMP are indirect, mediated through the induction of some cytokine(s) and/or growth factor(s) and that extracellular adenosine can act in cooperation with G-CSF. These findings contribute to the further elucidation of the role of adenosine in hematopoiesis.</description><subject>Adenosine - metabolism</subject><subject>Adenosine Monophosphate - metabolism</subject><subject>Adenosine Monophosphate - pharmacology</subject><subject>Animals</subject><subject>Bone marrow</subject><subject>Cells</subject><subject>Colony-Forming Units Assay</subject><subject>Dipyridamole - pharmacology</subject><subject>Female</subject><subject>Granulocyte Colony-Stimulating Factor - metabolism</subject><subject>Granulocyte Colony-Stimulating Factor - pharmacology</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - blood</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</subject><subject>Hematopoiesis - drug effects</subject><subject>Hematopoietic Stem Cells - drug effects</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Interleukin-6 - blood</subject><subject>Interleukin-6 - metabolism</subject><subject>Kinases</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Time Factors</subject><subject>Up-Regulation</subject><issn>0862-8408</issn><issn>1802-9973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkTtPwzAUhS0EouXxBxhQxMCW4lccZ0S8JSQWmCPHuQGXxC52XNGJv45LK1Viuh7O98lHB6EzgmeMFby6WnysgnG9hzCrGMGk2kNTIjHNq6pk-2iKpaC55FhO0FEIc4xpiUt2iCZEVLTgQkzRz62P7yGDHpZqNPY9g-_RKw19H3vlM9WCdcFYSK_BWBNG8NBmxmZLs3TptlFDFsDHIdOud3aVh9EMif2TKT2apRlXmbJrKME9xE9jc7FWDEbDCTroVB_gdHuP0dv93evNY_788vB0c_2ca0bZmDdF1RaU04KUuhSyYViBgk4qIA3WXZNSJQbgqqW8aaXCvOgoT9XbVFp1gh2jy4134d1XhDDWgwnrmsqCi6EWknLBMUvBi3_BuYvepr_VlFDKJMFrG92EtHcheOjqhTeD8qua4Ppvm3q3Tb3ZJkHnW3NsBmh3yHYM9gt_JZDJ</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Weiterová, L</creator><creator>Hofer, M</creator><creator>Pospísil, M</creator><creator>Znojil, V</creator><creator>Streitová, D</creator><general>Institute of Physiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice</title><author>Weiterová, L ; Hofer, M ; Pospísil, M ; Znojil, V ; Streitová, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-b59d5242517c768b30aeaef8ae1b0cfbc3270ee4ad24bd8a045f24840d002af63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenosine - metabolism</topic><topic>Adenosine Monophosphate - metabolism</topic><topic>Adenosine Monophosphate - pharmacology</topic><topic>Animals</topic><topic>Bone marrow</topic><topic>Cells</topic><topic>Colony-Forming Units Assay</topic><topic>Dipyridamole - pharmacology</topic><topic>Female</topic><topic>Granulocyte Colony-Stimulating Factor - metabolism</topic><topic>Granulocyte Colony-Stimulating Factor - pharmacology</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - blood</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</topic><topic>Hematopoiesis - drug effects</topic><topic>Hematopoietic Stem Cells - drug effects</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Interleukin-6 - blood</topic><topic>Interleukin-6 - metabolism</topic><topic>Kinases</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Proteins</topic><topic>Rodents</topic><topic>Time Factors</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weiterová, L</creatorcontrib><creatorcontrib>Hofer, M</creatorcontrib><creatorcontrib>Pospísil, M</creatorcontrib><creatorcontrib>Znojil, V</creatorcontrib><creatorcontrib>Streitová, D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Physiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weiterová, L</au><au>Hofer, M</au><au>Pospísil, M</au><au>Znojil, V</au><au>Streitová, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice</atitle><jtitle>Physiological research</jtitle><addtitle>Physiol Res</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>56</volume><issue>4</issue><spage>463</spage><epage>474</epage><pages>463-474</pages><issn>0862-8408</issn><eissn>1802-9973</eissn><abstract>Our previous studies have shown that the combined administration of drugs elevating extracellular adenosine, i.e. dipyridamole (DP) and adenosine monophosphate (AMP), enhances murine hematopoiesis and potentiates the action of granulocyte colony-stimulating factor (G-CSF). In this study, colony-stimulating activity (CSA) of blood serum of mice treated with DP+AMP, G-CSF or all these drugs in combination, i.e. the ability of the sera to stimulate the growth of GM-CFC colonies, was assayed in vitro. Furthermore, the concentration of GM-CSF and IL-6 in the sera was determined. Administration of DP+AMP was found to enhance significantly serum CSA at all time intervals of serum sampling including 24 h after the last injection of the tested drugs. Additive effects of DP+AMP and G-CSF on serum CSA were noted at early intervals after administration of the drugs. Furthermore, IL-6 levels were significantly elevated in the sera of mice which were administered DP+AMP either alone or in combination with G-CSF. Our results show that the effects of DP+AMP are indirect, mediated through the induction of some cytokine(s) and/or growth factor(s) and that extracellular adenosine can act in cooperation with G-CSF. These findings contribute to the further elucidation of the role of adenosine in hematopoiesis.</abstract><cop>Czech Republic</cop><pub>Institute of Physiology</pub><pmid>16925466</pmid><doi>10.33549/physiolres.931019</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0862-8408 |
| ispartof | Physiological research, 2007-01, Vol.56 (4), p.463-474 |
| issn | 0862-8408 1802-9973 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68246403 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adenosine - metabolism Adenosine Monophosphate - metabolism Adenosine Monophosphate - pharmacology Animals Bone marrow Cells Colony-Forming Units Assay Dipyridamole - pharmacology Female Granulocyte Colony-Stimulating Factor - metabolism Granulocyte Colony-Stimulating Factor - pharmacology Granulocyte-Macrophage Colony-Stimulating Factor - blood Granulocyte-Macrophage Colony-Stimulating Factor - metabolism Hematopoiesis - drug effects Hematopoietic Stem Cells - drug effects Hematopoietic Stem Cells - metabolism Interleukin-6 - blood Interleukin-6 - metabolism Kinases Male Mice Mice, Inbred ICR Proteins Rodents Time Factors Up-Regulation |
| title | Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T11%3A43%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Drugs%20elevating%20extracellular%20adenosine%20administered%20in%20vivo%20induce%20serum%20colony-stimulating%20activity%20and%20interleukin-6%20in%20mice&rft.jtitle=Physiological%20research&rft.au=Weiterov%C3%A1,%20L&rft.date=2007-01-01&rft.volume=56&rft.issue=4&rft.spage=463&rft.epage=474&rft.pages=463-474&rft.issn=0862-8408&rft.eissn=1802-9973&rft_id=info:doi/10.33549/physiolres.931019&rft_dat=%3Cproquest_cross%3E68246403%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=212238106&rft_id=info:pmid/16925466&rfr_iscdi=true |