Validation of a quantitative PCR diagnostic method for detection of the microsporidian Ovipleistophora ovariae in the cyprinid fish Notemigonus crysoleucas

Microsporidian parasites are easily detected by light microscopy when infections are heavy and spores are present. However, early infections without spores, or light infections with low numbers of spores, are easily missed. This limitation has made it difficult to conduct investigations into microsp...

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Veröffentlicht in:	Diseases of aquatic organisms 2007-07, Vol.76 (3), p.215-221
Hauptverfasser:	PHELPS, Nicholas B. D, GOODWIN, Andrew E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal, plant and microbial ecology Animals Base Sequence Biological and medical sciences Cyprinidae DNA, Fungal - chemistry DNA, Fungal - genetics Female Fish Diseases - diagnosis Fish Diseases - microbiology Freshwater Fundamental and applied biological sciences. Psychology General aspects. Techniques Glugea stephani Male Methods and techniques (sampling, tagging, trapping, modelling...) Microsporidia - genetics Microsporidia - isolation & purification Microsporidiosis - diagnosis Microsporidiosis - microbiology Microsporidiosis - veterinary Molecular Sequence Data Notemigonus crysoleucas Nucleospora salmonis Ovipleistophora Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Sensitivity and Specificity Sequence Alignment Spores, Fungal - genetics Spores, Fungal - isolation & purification
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