Cloning and characterization of the chromosomal arsenic resistance genes from Acidithiobacillus caldus and enhanced arsenic resistance on conjugal transfer of ars genes located on transposon TnAtcArs
Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa Correspondence Douglas E. Rawlings der{at}sun.ac.za All strains of the moderately thermophilic, acidophilic, sulphur-oxidizing bacterium Acidithiobacillus caldus that have been tested contain a set o...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2006-12, Vol.152 (12), p.3551-3560 |
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| creator | Kotze, Andre A Tuffin, I. Marla Deane, Shelly M Rawlings, Douglas E |
| description | Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
Correspondence Douglas E. Rawlings der{at}sun.ac.za
All strains of the moderately thermophilic, acidophilic, sulphur-oxidizing bacterium Acidithiobacillus caldus that have been tested contain a set of chromosomal arsenic resistance genes. Highly arsenic-resistant strains isolated from commercial arsenopyrite bio-oxidation tanks contain additional transposon-located (Tn AtcArs ) arsenic resistance genes. The chromosomal At. caldus ars genes were cloned and found to consist of arsR and arsC genes transcribed in one direction, and arsB in the opposite direction. The arsRC genes were co-transcribed with ORF1, and arsB with ORF5 in both At. caldus and Escherichia coli , although deletion of ORFs 1 and 5 did not appear to affect resistance to arsenate or arsenite in E. coli . ORFs 1 and 5 have not previously been reported as part of the ars operons, and had high amino acid identity to hypothetical proteins from Polaromonas naphthalenivorus (76 %) and Legionella pneumophila (60 %), respectively. Reporter-gene studies showed that the arsenic operon of transposon origin (Tn AtcArs ) was expressed at a higher level, and was less tightly regulated in E. coli than were the At. caldus ars genes of chromosomal origin. Plasmid pSa-mediated conjugal transfer of Tn AtcArs from E. coli to At. caldus strains lacking the transposon was successful, and resulted in greatly increased levels of resistance to arsenite.
Abbreviations: NCBI, National Centre for Biotechnology Information
The GenBank/EMBL/DDBJ accession number for the sequence of the insert of pAtcars4 reported in this paper is DQ810790. |
| doi_str_mv | 10.1099/mic.0.29247-0 |
| format | Article |
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Correspondence Douglas E. Rawlings der{at}sun.ac.za
All strains of the moderately thermophilic, acidophilic, sulphur-oxidizing bacterium Acidithiobacillus caldus that have been tested contain a set of chromosomal arsenic resistance genes. Highly arsenic-resistant strains isolated from commercial arsenopyrite bio-oxidation tanks contain additional transposon-located (Tn AtcArs ) arsenic resistance genes. The chromosomal At. caldus ars genes were cloned and found to consist of arsR and arsC genes transcribed in one direction, and arsB in the opposite direction. The arsRC genes were co-transcribed with ORF1, and arsB with ORF5 in both At. caldus and Escherichia coli , although deletion of ORFs 1 and 5 did not appear to affect resistance to arsenate or arsenite in E. coli . ORFs 1 and 5 have not previously been reported as part of the ars operons, and had high amino acid identity to hypothetical proteins from Polaromonas naphthalenivorus (76 %) and Legionella pneumophila (60 %), respectively. Reporter-gene studies showed that the arsenic operon of transposon origin (Tn AtcArs ) was expressed at a higher level, and was less tightly regulated in E. coli than were the At. caldus ars genes of chromosomal origin. Plasmid pSa-mediated conjugal transfer of Tn AtcArs from E. coli to At. caldus strains lacking the transposon was successful, and resulted in greatly increased levels of resistance to arsenite.
Abbreviations: NCBI, National Centre for Biotechnology Information
The GenBank/EMBL/DDBJ accession number for the sequence of the insert of pAtcars4 reported in this paper is DQ810790.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/mic.0.29247-0</identifier><identifier>PMID: 17159207</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Acidithiobacillus - drug effects ; Acidithiobacillus - genetics ; Acidithiobacillus caldus ; Action of physical and chemical agents on bacteria ; Anti-Bacterial Agents - pharmacology ; Arsenates - pharmacology ; Arsenites - pharmacology ; Bacteriology ; Biological and medical sciences ; Chromosomes, Bacterial - genetics ; Cloning, Molecular ; Comamonadaceae - genetics ; Conjugation, Genetic ; DNA Transposable Elements - genetics ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Drug Resistance, Bacterial - genetics ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Gene Transfer, Horizontal ; Genes, Bacterial ; Genetics ; Legionella pneumophila ; Legionella pneumophila - genetics ; Metabolism. Enzymes ; Microbiology ; Molecular Sequence Data ; Open Reading Frames ; Operon ; R Factors - genetics ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Transcription, Genetic</subject><ispartof>Microbiology (Society for General Microbiology), 2006-12, Vol.152 (12), p.3551-3560</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-76d76155befd1cb3f6252d2bc71ffffe7569271aea763aba429eb4139eee06c3</citedby><cites>FETCH-LOGICAL-c426t-76d76155befd1cb3f6252d2bc71ffffe7569271aea763aba429eb4139eee06c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18380982$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17159207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kotze, Andre A</creatorcontrib><creatorcontrib>Tuffin, I. Marla</creatorcontrib><creatorcontrib>Deane, Shelly M</creatorcontrib><creatorcontrib>Rawlings, Douglas E</creatorcontrib><title>Cloning and characterization of the chromosomal arsenic resistance genes from Acidithiobacillus caldus and enhanced arsenic resistance on conjugal transfer of ars genes located on transposon TnAtcArs</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
Correspondence Douglas E. Rawlings der{at}sun.ac.za
All strains of the moderately thermophilic, acidophilic, sulphur-oxidizing bacterium Acidithiobacillus caldus that have been tested contain a set of chromosomal arsenic resistance genes. Highly arsenic-resistant strains isolated from commercial arsenopyrite bio-oxidation tanks contain additional transposon-located (Tn AtcArs ) arsenic resistance genes. The chromosomal At. caldus ars genes were cloned and found to consist of arsR and arsC genes transcribed in one direction, and arsB in the opposite direction. The arsRC genes were co-transcribed with ORF1, and arsB with ORF5 in both At. caldus and Escherichia coli , although deletion of ORFs 1 and 5 did not appear to affect resistance to arsenate or arsenite in E. coli . ORFs 1 and 5 have not previously been reported as part of the ars operons, and had high amino acid identity to hypothetical proteins from Polaromonas naphthalenivorus (76 %) and Legionella pneumophila (60 %), respectively. Reporter-gene studies showed that the arsenic operon of transposon origin (Tn AtcArs ) was expressed at a higher level, and was less tightly regulated in E. coli than were the At. caldus ars genes of chromosomal origin. Plasmid pSa-mediated conjugal transfer of Tn AtcArs from E. coli to At. caldus strains lacking the transposon was successful, and resulted in greatly increased levels of resistance to arsenite.
Abbreviations: NCBI, National Centre for Biotechnology Information
The GenBank/EMBL/DDBJ accession number for the sequence of the insert of pAtcars4 reported in this paper is DQ810790.</description><subject>Acidithiobacillus - drug effects</subject><subject>Acidithiobacillus - genetics</subject><subject>Acidithiobacillus caldus</subject><subject>Action of physical and chemical agents on bacteria</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Arsenates - pharmacology</subject><subject>Arsenites - pharmacology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromosomes, Bacterial - genetics</subject><subject>Cloning, Molecular</subject><subject>Comamonadaceae - genetics</subject><subject>Conjugation, Genetic</subject><subject>DNA Transposable Elements - genetics</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Transfer, Horizontal</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Legionella pneumophila</subject><subject>Legionella pneumophila - genetics</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Operon</subject><subject>R Factors - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription, Genetic</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c-L3CAUB_BQWrrbbY-9Fi_tYSFTNTHG4zD0Fyz0Mncx5mXikuhUDaX7D-6_1ZedwF4KzcWgH79P3iuK94zuGFXq8-zsju644rUs6YvimtWNKDlt6Uv8rwQtaSv5VfEmpXtK8ZCy18UVk0woTuV18XiYgnf-RIzviR1NNDZDdA8mu-BJGEgeAfdjmEMKs5mIiQm8syRCcikbb4GcwEMiAxqyt653eXShM9ZN05KINVOPyxoPflx9_68MLGaDv19OWCJH49MAcS2PdMufgjUZL6N8Amd8kCdHv892H9Pb4tVgpgTvtvWmOH79cjx8L-9-fvtx2N-VtuZNLmXTy4YJ0cHQM9tVQ8MF73lnJRvwAykaxSUzYGRTmc7UXEFXs0oBAG1sdVN8usSeY_i1QMp6dsnCNBkPYUm6aTnqRv0XMtUKLqhAWF6gjSGlCIM-Rzeb-EczqtcJ40WrqX6asKboP2zBSzdD_6y3kSL4uAGTsPkD9sq69OzaqqWq5ehuL250p_G3i6Cxz1grhs6FtSgTXDOuKyFY9RcJg8Nz</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Kotze, Andre A</creator><creator>Tuffin, I. Marla</creator><creator>Deane, Shelly M</creator><creator>Rawlings, Douglas E</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Cloning and characterization of the chromosomal arsenic resistance genes from Acidithiobacillus caldus and enhanced arsenic resistance on conjugal transfer of ars genes located on transposon TnAtcArs</title><author>Kotze, Andre A ; Tuffin, I. Marla ; Deane, Shelly M ; Rawlings, Douglas E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-76d76155befd1cb3f6252d2bc71ffffe7569271aea763aba429eb4139eee06c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acidithiobacillus - drug effects</topic><topic>Acidithiobacillus - genetics</topic><topic>Acidithiobacillus caldus</topic><topic>Action of physical and chemical agents on bacteria</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Arsenates - pharmacology</topic><topic>Arsenites - pharmacology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chromosomes, Bacterial - genetics</topic><topic>Cloning, Molecular</topic><topic>Comamonadaceae - genetics</topic><topic>Conjugation, Genetic</topic><topic>DNA Transposable Elements - genetics</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Transfer, Horizontal</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Legionella pneumophila</topic><topic>Legionella pneumophila - genetics</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>Operon</topic><topic>R Factors - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kotze, Andre A</creatorcontrib><creatorcontrib>Tuffin, I. Marla</creatorcontrib><creatorcontrib>Deane, Shelly M</creatorcontrib><creatorcontrib>Rawlings, Douglas E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kotze, Andre A</au><au>Tuffin, I. Marla</au><au>Deane, Shelly M</au><au>Rawlings, Douglas E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of the chromosomal arsenic resistance genes from Acidithiobacillus caldus and enhanced arsenic resistance on conjugal transfer of ars genes located on transposon TnAtcArs</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>152</volume><issue>12</issue><spage>3551</spage><epage>3560</epage><pages>3551-3560</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
Correspondence Douglas E. Rawlings der{at}sun.ac.za
All strains of the moderately thermophilic, acidophilic, sulphur-oxidizing bacterium Acidithiobacillus caldus that have been tested contain a set of chromosomal arsenic resistance genes. Highly arsenic-resistant strains isolated from commercial arsenopyrite bio-oxidation tanks contain additional transposon-located (Tn AtcArs ) arsenic resistance genes. The chromosomal At. caldus ars genes were cloned and found to consist of arsR and arsC genes transcribed in one direction, and arsB in the opposite direction. The arsRC genes were co-transcribed with ORF1, and arsB with ORF5 in both At. caldus and Escherichia coli , although deletion of ORFs 1 and 5 did not appear to affect resistance to arsenate or arsenite in E. coli . ORFs 1 and 5 have not previously been reported as part of the ars operons, and had high amino acid identity to hypothetical proteins from Polaromonas naphthalenivorus (76 %) and Legionella pneumophila (60 %), respectively. Reporter-gene studies showed that the arsenic operon of transposon origin (Tn AtcArs ) was expressed at a higher level, and was less tightly regulated in E. coli than were the At. caldus ars genes of chromosomal origin. Plasmid pSa-mediated conjugal transfer of Tn AtcArs from E. coli to At. caldus strains lacking the transposon was successful, and resulted in greatly increased levels of resistance to arsenite.
Abbreviations: NCBI, National Centre for Biotechnology Information
The GenBank/EMBL/DDBJ accession number for the sequence of the insert of pAtcars4 reported in this paper is DQ810790.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>17159207</pmid><doi>10.1099/mic.0.29247-0</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acidithiobacillus - drug effects Acidithiobacillus - genetics Acidithiobacillus caldus Action of physical and chemical agents on bacteria Anti-Bacterial Agents - pharmacology Arsenates - pharmacology Arsenites - pharmacology Bacteriology Biological and medical sciences Chromosomes, Bacterial - genetics Cloning, Molecular Comamonadaceae - genetics Conjugation, Genetic DNA Transposable Elements - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics Drug Resistance, Bacterial - genetics Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Deletion Gene Expression Regulation, Bacterial Gene Transfer, Horizontal Genes, Bacterial Genetics Legionella pneumophila Legionella pneumophila - genetics Metabolism. Enzymes Microbiology Molecular Sequence Data Open Reading Frames Operon R Factors - genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic |
| title | Cloning and characterization of the chromosomal arsenic resistance genes from Acidithiobacillus caldus and enhanced arsenic resistance on conjugal transfer of ars genes located on transposon TnAtcArs |
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