Augmented induction of CD8+ cytotoxic T-cell response and antitumor effect by DCs pulsed with virus-like particles packaging with CpG
Abstract The present study aims at establishing a novel vaccine procedure based on HBc-VLP-pulsed DCs. Immature mice BMDCs could capture HBc-VLP or HBc-VLP packaging CpG efficiently and present the antigen to syngeneic mice spleen T cells in vitro . Immunization with DCs showed that compared to VLP-...
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Veröffentlicht in: | Cancer letters 2007-10, Vol.256 (1), p.90-100 |
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description | Abstract The present study aims at establishing a novel vaccine procedure based on HBc-VLP-pulsed DCs. Immature mice BMDCs could capture HBc-VLP or HBc-VLP packaging CpG efficiently and present the antigen to syngeneic mice spleen T cells in vitro . Immunization with DCs showed that compared to VLP-pulsed DCs, VLP packaging CpG-pulsed DCs elicit stronger T-cell responses in vivo , as measured by both intracellular production of IFN-γ and in vivo killing assays by Ag-specific T cells. In the B16-pIR-HH tumor therapy model, the growth of established tumors was significantly inhibited by single immunization of DCs pulsed with HBc-VLP packaged with CpG, resulting in significantly longer survival of immunized animals and strikingly, high frequencies (>10% of CD8+ cells) of protective CTL could be induced and maintained. The mice immunized with DCs treated with HBc-VLP, however, trigger an antitumor effect at the early phase of vaccination, after 20 days of tumor injection, the tumor growth inhibition of VLP-pulsed DCs vaccination was decreased gradually and the fact could be interpreted by the decreasing number of antigen-specific CD8+ T-cell and IFN-γ+ -producing CD8+ T cell. This study therefore shows that the use of HBc-VLP packaging CpG-pulsed DCs could facilitate the development of effective T-cell-based vaccines. |
doi_str_mv | 10.1016/j.canlet.2007.06.004 |
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Immature mice BMDCs could capture HBc-VLP or HBc-VLP packaging CpG efficiently and present the antigen to syngeneic mice spleen T cells in vitro . Immunization with DCs showed that compared to VLP-pulsed DCs, VLP packaging CpG-pulsed DCs elicit stronger T-cell responses in vivo , as measured by both intracellular production of IFN-γ and in vivo killing assays by Ag-specific T cells. In the B16-pIR-HH tumor therapy model, the growth of established tumors was significantly inhibited by single immunization of DCs pulsed with HBc-VLP packaged with CpG, resulting in significantly longer survival of immunized animals and strikingly, high frequencies (>10% of CD8+ cells) of protective CTL could be induced and maintained. The mice immunized with DCs treated with HBc-VLP, however, trigger an antitumor effect at the early phase of vaccination, after 20 days of tumor injection, the tumor growth inhibition of VLP-pulsed DCs vaccination was decreased gradually and the fact could be interpreted by the decreasing number of antigen-specific CD8+ T-cell and IFN-γ+ -producing CD8+ T cell. This study therefore shows that the use of HBc-VLP packaging CpG-pulsed DCs could facilitate the development of effective T-cell-based vaccines.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2007.06.004</identifier><identifier>PMID: 17656012</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Antitumoral experiment ; Bone marrow ; Cancer Vaccines - immunology ; CD8-Positive T-Lymphocytes - immunology ; Cell Proliferation ; CpG ; CTL ; DCs ; Dendritic Cells - immunology ; Dendritic Cells - virology ; HBc-VLP ; Hematology, Oncology and Palliative Medicine ; Hepatitis B - immunology ; Hepatitis B - pathology ; Hepatitis B - therapy ; Hepatitis B Core Antigens - immunology ; Hepatitis B virus - immunology ; Humans ; Immune system ; Immunization ; Immunotherapy ; Interferon-gamma - metabolism ; Lymphocytes ; Mice ; Mice, Transgenic ; Molecular weight ; Neoplasms - immunology ; Neoplasms - therapy ; Oligodeoxyribonucleotides ; Peptides ; T cell receptors ; T-Lymphocytes - immunology ; T-Lymphocytes - metabolism ; T-Lymphocytes - virology ; T-Lymphocytes, Cytotoxic - immunology ; Transmission electron microscopy ; Tumors ; Vaccines ; Virion - immunology</subject><ispartof>Cancer letters, 2007-10, Vol.256 (1), p.90-100</ispartof><rights>2007</rights><rights>Copyright Elsevier Limited Oct 18, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-264c48792f51d6b7e2ef8b93bb72b812041c92ac207258c488b585445b8dd3293</citedby><cites>FETCH-LOGICAL-c474t-264c48792f51d6b7e2ef8b93bb72b812041c92ac207258c488b585445b8dd3293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.canlet.2007.06.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17656012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Shuxia</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>He, Ying</creatorcontrib><creatorcontrib>Ren, Ding</creatorcontrib><creatorcontrib>Guo, Yingjun</creatorcontrib><creatorcontrib>Sun, Shuhan</creatorcontrib><title>Augmented induction of CD8+ cytotoxic T-cell response and antitumor effect by DCs pulsed with virus-like particles packaging with CpG</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Abstract The present study aims at establishing a novel vaccine procedure based on HBc-VLP-pulsed DCs. Immature mice BMDCs could capture HBc-VLP or HBc-VLP packaging CpG efficiently and present the antigen to syngeneic mice spleen T cells in vitro . Immunization with DCs showed that compared to VLP-pulsed DCs, VLP packaging CpG-pulsed DCs elicit stronger T-cell responses in vivo , as measured by both intracellular production of IFN-γ and in vivo killing assays by Ag-specific T cells. In the B16-pIR-HH tumor therapy model, the growth of established tumors was significantly inhibited by single immunization of DCs pulsed with HBc-VLP packaged with CpG, resulting in significantly longer survival of immunized animals and strikingly, high frequencies (>10% of CD8+ cells) of protective CTL could be induced and maintained. The mice immunized with DCs treated with HBc-VLP, however, trigger an antitumor effect at the early phase of vaccination, after 20 days of tumor injection, the tumor growth inhibition of VLP-pulsed DCs vaccination was decreased gradually and the fact could be interpreted by the decreasing number of antigen-specific CD8+ T-cell and IFN-γ+ -producing CD8+ T cell. This study therefore shows that the use of HBc-VLP packaging CpG-pulsed DCs could facilitate the development of effective T-cell-based vaccines.</description><subject>Animals</subject><subject>Antitumoral experiment</subject><subject>Bone marrow</subject><subject>Cancer Vaccines - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cell Proliferation</subject><subject>CpG</subject><subject>CTL</subject><subject>DCs</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - virology</subject><subject>HBc-VLP</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Hepatitis B - immunology</subject><subject>Hepatitis B - pathology</subject><subject>Hepatitis B - therapy</subject><subject>Hepatitis B Core Antigens - immunology</subject><subject>Hepatitis B virus - immunology</subject><subject>Humans</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Immunotherapy</subject><subject>Interferon-gamma - metabolism</subject><subject>Lymphocytes</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Molecular weight</subject><subject>Neoplasms - immunology</subject><subject>Neoplasms - therapy</subject><subject>Oligodeoxyribonucleotides</subject><subject>Peptides</subject><subject>T cell receptors</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - metabolism</subject><subject>T-Lymphocytes - virology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Transmission electron microscopy</subject><subject>Tumors</subject><subject>Vaccines</subject><subject>Virion - immunology</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkFr3DAQhU1pabZp_0EpgkIuxduRLFnaSyFsmqQQ6KHpWdjyeKtdr-RKctr9Af3fldmFQC45iLl874k3b4riPYUlBVp_3i5N4wZMSwYgl1AvAfiLYkGVZKVcKXhZLKACXlaqEmfFmxi3ACC4FK-LMyprUQNli-Lf5bTZo0vYEeu6ySTrHfE9WV-pT8Qckk_-rzXkvjQ4DCRgHL2LSBrX5ZdsmvY-EOx7NIm0B3K1jmSchpjt_tj0izzYMMVysDskYxOSNQNmoDG7ZmPd5sisx5u3xau-yap3p3le_Lz-er--Le--33xbX96VhkueSlZzw5VcsV7Qrm4lMuxVu6raVrJWUQacmhVrDAPJhMqoaoUSnItWdV3FVtV5cXH0HYP_PWFMem_jnKxx6Keoa8U4MBDPglRK4JVkGfz4BNz6KbgcQlMxr5tTrjLFj5QJPsaAvR6D3TfhoCnouU291cc29dymhlrnNrPsw8l8avfYPYpO9WXgyxHAvLQHi0FHY9EZ7GzIjejO2-d-eGpgBuusaYYdHjA-ZtGRadA_5ouaDwokAFOcV_8B2bHGTw</recordid><startdate>20071018</startdate><enddate>20071018</enddate><creator>Song, Shuxia</creator><creator>Wang, Yue</creator><creator>Zhang, Yan</creator><creator>Wang, Fang</creator><creator>He, Ying</creator><creator>Ren, Ding</creator><creator>Guo, Yingjun</creator><creator>Sun, Shuhan</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071018</creationdate><title>Augmented induction of CD8+ cytotoxic T-cell response and antitumor effect by DCs pulsed with virus-like particles packaging with CpG</title><author>Song, Shuxia ; Wang, Yue ; Zhang, Yan ; Wang, Fang ; He, Ying ; Ren, Ding ; Guo, Yingjun ; Sun, Shuhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-264c48792f51d6b7e2ef8b93bb72b812041c92ac207258c488b585445b8dd3293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antitumoral experiment</topic><topic>Bone marrow</topic><topic>Cancer Vaccines - immunology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cell Proliferation</topic><topic>CpG</topic><topic>CTL</topic><topic>DCs</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - virology</topic><topic>HBc-VLP</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Hepatitis B - immunology</topic><topic>Hepatitis B - pathology</topic><topic>Hepatitis B - therapy</topic><topic>Hepatitis B Core Antigens - immunology</topic><topic>Hepatitis B virus - immunology</topic><topic>Humans</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Immunotherapy</topic><topic>Interferon-gamma - metabolism</topic><topic>Lymphocytes</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Molecular weight</topic><topic>Neoplasms - immunology</topic><topic>Neoplasms - therapy</topic><topic>Oligodeoxyribonucleotides</topic><topic>Peptides</topic><topic>T cell receptors</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - metabolism</topic><topic>T-Lymphocytes - virology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Transmission electron microscopy</topic><topic>Tumors</topic><topic>Vaccines</topic><topic>Virion - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Shuxia</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>He, Ying</creatorcontrib><creatorcontrib>Ren, Ding</creatorcontrib><creatorcontrib>Guo, Yingjun</creatorcontrib><creatorcontrib>Sun, Shuhan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Shuxia</au><au>Wang, Yue</au><au>Zhang, Yan</au><au>Wang, Fang</au><au>He, Ying</au><au>Ren, Ding</au><au>Guo, Yingjun</au><au>Sun, Shuhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Augmented induction of CD8+ cytotoxic T-cell response and antitumor effect by DCs pulsed with virus-like particles packaging with CpG</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2007-10-18</date><risdate>2007</risdate><volume>256</volume><issue>1</issue><spage>90</spage><epage>100</epage><pages>90-100</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Abstract The present study aims at establishing a novel vaccine procedure based on HBc-VLP-pulsed DCs. Immature mice BMDCs could capture HBc-VLP or HBc-VLP packaging CpG efficiently and present the antigen to syngeneic mice spleen T cells in vitro . Immunization with DCs showed that compared to VLP-pulsed DCs, VLP packaging CpG-pulsed DCs elicit stronger T-cell responses in vivo , as measured by both intracellular production of IFN-γ and in vivo killing assays by Ag-specific T cells. In the B16-pIR-HH tumor therapy model, the growth of established tumors was significantly inhibited by single immunization of DCs pulsed with HBc-VLP packaged with CpG, resulting in significantly longer survival of immunized animals and strikingly, high frequencies (>10% of CD8+ cells) of protective CTL could be induced and maintained. The mice immunized with DCs treated with HBc-VLP, however, trigger an antitumor effect at the early phase of vaccination, after 20 days of tumor injection, the tumor growth inhibition of VLP-pulsed DCs vaccination was decreased gradually and the fact could be interpreted by the decreasing number of antigen-specific CD8+ T-cell and IFN-γ+ -producing CD8+ T cell. This study therefore shows that the use of HBc-VLP packaging CpG-pulsed DCs could facilitate the development of effective T-cell-based vaccines.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>17656012</pmid><doi>10.1016/j.canlet.2007.06.004</doi><tpages>11</tpages></addata></record> |
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subjects | Animals Antitumoral experiment Bone marrow Cancer Vaccines - immunology CD8-Positive T-Lymphocytes - immunology Cell Proliferation CpG CTL DCs Dendritic Cells - immunology Dendritic Cells - virology HBc-VLP Hematology, Oncology and Palliative Medicine Hepatitis B - immunology Hepatitis B - pathology Hepatitis B - therapy Hepatitis B Core Antigens - immunology Hepatitis B virus - immunology Humans Immune system Immunization Immunotherapy Interferon-gamma - metabolism Lymphocytes Mice Mice, Transgenic Molecular weight Neoplasms - immunology Neoplasms - therapy Oligodeoxyribonucleotides Peptides T cell receptors T-Lymphocytes - immunology T-Lymphocytes - metabolism T-Lymphocytes - virology T-Lymphocytes, Cytotoxic - immunology Transmission electron microscopy Tumors Vaccines Virion - immunology |
title | Augmented induction of CD8+ cytotoxic T-cell response and antitumor effect by DCs pulsed with virus-like particles packaging with CpG |
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