Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides
Abstract Cationically modified poly( d , l -lactide- co -glycolide) (PLGA) nanoparticles have recently been introduced as novel carriers for DNA/RNA delivery. The colloidal characteristics of the nanoparticles—particle size and surface charge—are considered the most significant determinants in the c...
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| Veröffentlicht in: | Nanomedicine 2007-09, Vol.3 (3), p.173-183 |
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| creator | Nafee, Noha, PharmD Taetz, Sebastian, PharmD Schneider, Marc, PhD Schaefer, Ulrich F., PhD Lehr, Claus-Michael, PhD |
| description | Abstract Cationically modified poly( d , l -lactide- co -glycolide) (PLGA) nanoparticles have recently been introduced as novel carriers for DNA/RNA delivery. The colloidal characteristics of the nanoparticles—particle size and surface charge—are considered the most significant determinants in the cellular uptake and trafficking of the nanoparticles. Therefore, our aim was to introduce chitosan-coated PLGA nanoparticles, whose size and charge are tunable to adapt for a specific task. The results showed that biodegradable nanoparticles as small as 130 nm and adjustable surface charge can be tailored controlling the process parameters. As a proof of concept, the overall potential of these particulate carriers to bind the antisense oligonucleotides, 2′- O -methyl-RNA, and improve their cellular uptake was demonstrated. The study proved the efficacy of chitosan-coated PLGA nanoparticles as a flexible and efficient delivery system for antisense oligonucleotides to lung cancer cells. |
| doi_str_mv | 10.1016/j.nano.2007.03.006 |
| format | Article |
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The colloidal characteristics of the nanoparticles—particle size and surface charge—are considered the most significant determinants in the cellular uptake and trafficking of the nanoparticles. Therefore, our aim was to introduce chitosan-coated PLGA nanoparticles, whose size and charge are tunable to adapt for a specific task. The results showed that biodegradable nanoparticles as small as 130 nm and adjustable surface charge can be tailored controlling the process parameters. As a proof of concept, the overall potential of these particulate carriers to bind the antisense oligonucleotides, 2′- O -methyl-RNA, and improve their cellular uptake was demonstrated. The study proved the efficacy of chitosan-coated PLGA nanoparticles as a flexible and efficient delivery system for antisense oligonucleotides to lung cancer cells.</description><identifier>ISSN: 1549-9634</identifier><identifier>EISSN: 1549-9642</identifier><identifier>DOI: 10.1016/j.nano.2007.03.006</identifier><identifier>PMID: 17692575</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antisense oligonucleotides ; Cell Line, Tumor ; Chitosan ; Chitosan - chemistry ; Coated Materials, Biocompatible ; Crystallization - methods ; DNA - administration & dosage ; DNA - genetics ; DNA - pharmacokinetics ; Drug Carriers - chemistry ; Drug Compounding - methods ; Gene delivery ; Humans ; Internal Medicine ; Lactic Acid - chemistry ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Materials Testing ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Particle Size ; PLGA nanoparticles ; Polyglycolic Acid - chemistry ; Polymers - chemistry ; RNA, Antisense - administration & dosage ; RNA, Antisense - genetics ; RNA, Antisense - pharmacokinetics ; Telomerase inhibitors ; Transfection - methods</subject><ispartof>Nanomedicine, 2007-09, Vol.3 (3), p.173-183</ispartof><rights>Elsevier Inc.</rights><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-3b815cbe7c58be156d81831d367ae288cbd78c3b28e383f6690dbb5d8afb4ed83</citedby><cites>FETCH-LOGICAL-c506t-3b815cbe7c58be156d81831d367ae288cbd78c3b28e383f6690dbb5d8afb4ed83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.nano.2007.03.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17692575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nafee, Noha, PharmD</creatorcontrib><creatorcontrib>Taetz, Sebastian, PharmD</creatorcontrib><creatorcontrib>Schneider, Marc, PhD</creatorcontrib><creatorcontrib>Schaefer, Ulrich F., PhD</creatorcontrib><creatorcontrib>Lehr, Claus-Michael, PhD</creatorcontrib><title>Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides</title><title>Nanomedicine</title><addtitle>Nanomedicine</addtitle><description>Abstract Cationically modified poly( d , l -lactide- co -glycolide) (PLGA) nanoparticles have recently been introduced as novel carriers for DNA/RNA delivery. The colloidal characteristics of the nanoparticles—particle size and surface charge—are considered the most significant determinants in the cellular uptake and trafficking of the nanoparticles. Therefore, our aim was to introduce chitosan-coated PLGA nanoparticles, whose size and charge are tunable to adapt for a specific task. The results showed that biodegradable nanoparticles as small as 130 nm and adjustable surface charge can be tailored controlling the process parameters. As a proof of concept, the overall potential of these particulate carriers to bind the antisense oligonucleotides, 2′- O -methyl-RNA, and improve their cellular uptake was demonstrated. The study proved the efficacy of chitosan-coated PLGA nanoparticles as a flexible and efficient delivery system for antisense oligonucleotides to lung cancer cells.</description><subject>Antisense oligonucleotides</subject><subject>Cell Line, Tumor</subject><subject>Chitosan</subject><subject>Chitosan - chemistry</subject><subject>Coated Materials, Biocompatible</subject><subject>Crystallization - methods</subject><subject>DNA - administration & dosage</subject><subject>DNA - genetics</subject><subject>DNA - pharmacokinetics</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Compounding - methods</subject><subject>Gene delivery</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Lactic Acid - chemistry</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Materials Testing</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Particle Size</subject><subject>PLGA nanoparticles</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Polymers - chemistry</subject><subject>RNA, Antisense - administration & dosage</subject><subject>RNA, Antisense - genetics</subject><subject>RNA, Antisense - pharmacokinetics</subject><subject>Telomerase inhibitors</subject><subject>Transfection - methods</subject><issn>1549-9634</issn><issn>1549-9642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksuKFDEYhQtRnHH0BVxIVu6qJpeuVEpEaFodhWYUL-uQSv5y0qaSniQ12O_iw5qiGwUXusrtfCdwzl9VTwluCCb8ctd45UNDMe4azBqM-b3qnLSrvu75it7_vWers-pRSjuMWYdx_7A6Ix3vadu159XPzY3NISlf66AyGPRxe7VGi-9exWy1g4TGENHr6_Xlp-s1MuDsHcTDCwTjCDqjMKJ8A4tmmp3KNnhUSDVBhphQOekw7R38OD4pb1COyqeFXS4Krny2CXwCFJz9FvxcPg3ZGkiPqwejcgmenNaL6uvbN1827-rth6v3m_W21i3muWaDIK0eoNOtGIC03AgiGDGMdwqoEHowndBsoAKYYCPnPTbD0BqhxmEFRrCL6vnRdx_D7Qwpy8kmDc4pD2FOkgvKaDH6r5D0XPSU9kVIj0IdQ0oRRrmPdlLxIAmWS3lyJ5eQ5VKexEyW8gr07OQ-DxOYP8iprSJ4eRRACePOQpRJW_AajI0lT2mC_bf_q79w7ay3WrnvcIC0C3P0JWZJZKISy8_L-CzTg8vUYNG37BcAlsPC</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Nafee, Noha, PharmD</creator><creator>Taetz, Sebastian, PharmD</creator><creator>Schneider, Marc, PhD</creator><creator>Schaefer, Ulrich F., PhD</creator><creator>Lehr, Claus-Michael, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070901</creationdate><title>Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides</title><author>Nafee, Noha, PharmD ; Taetz, Sebastian, PharmD ; Schneider, Marc, PhD ; Schaefer, Ulrich F., PhD ; Lehr, Claus-Michael, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-3b815cbe7c58be156d81831d367ae288cbd78c3b28e383f6690dbb5d8afb4ed83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Antisense oligonucleotides</topic><topic>Cell Line, Tumor</topic><topic>Chitosan</topic><topic>Chitosan - chemistry</topic><topic>Coated Materials, Biocompatible</topic><topic>Crystallization - methods</topic><topic>DNA - administration & dosage</topic><topic>DNA - genetics</topic><topic>DNA - pharmacokinetics</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Compounding - methods</topic><topic>Gene delivery</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Lactic Acid - chemistry</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Materials Testing</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - ultrastructure</topic><topic>Particle Size</topic><topic>PLGA nanoparticles</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Polymers - chemistry</topic><topic>RNA, Antisense - administration & dosage</topic><topic>RNA, Antisense - genetics</topic><topic>RNA, Antisense - pharmacokinetics</topic><topic>Telomerase inhibitors</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nafee, Noha, PharmD</creatorcontrib><creatorcontrib>Taetz, Sebastian, PharmD</creatorcontrib><creatorcontrib>Schneider, Marc, PhD</creatorcontrib><creatorcontrib>Schaefer, Ulrich F., PhD</creatorcontrib><creatorcontrib>Lehr, Claus-Michael, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nafee, Noha, PharmD</au><au>Taetz, Sebastian, PharmD</au><au>Schneider, Marc, PhD</au><au>Schaefer, Ulrich F., PhD</au><au>Lehr, Claus-Michael, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides</atitle><jtitle>Nanomedicine</jtitle><addtitle>Nanomedicine</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>3</volume><issue>3</issue><spage>173</spage><epage>183</epage><pages>173-183</pages><issn>1549-9634</issn><eissn>1549-9642</eissn><abstract>Abstract Cationically modified poly( d , l -lactide- co -glycolide) (PLGA) nanoparticles have recently been introduced as novel carriers for DNA/RNA delivery. The colloidal characteristics of the nanoparticles—particle size and surface charge—are considered the most significant determinants in the cellular uptake and trafficking of the nanoparticles. Therefore, our aim was to introduce chitosan-coated PLGA nanoparticles, whose size and charge are tunable to adapt for a specific task. The results showed that biodegradable nanoparticles as small as 130 nm and adjustable surface charge can be tailored controlling the process parameters. As a proof of concept, the overall potential of these particulate carriers to bind the antisense oligonucleotides, 2′- O -methyl-RNA, and improve their cellular uptake was demonstrated. The study proved the efficacy of chitosan-coated PLGA nanoparticles as a flexible and efficient delivery system for antisense oligonucleotides to lung cancer cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17692575</pmid><doi>10.1016/j.nano.2007.03.006</doi><tpages>11</tpages></addata></record> |
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| subjects | Antisense oligonucleotides Cell Line, Tumor Chitosan Chitosan - chemistry Coated Materials, Biocompatible Crystallization - methods DNA - administration & dosage DNA - genetics DNA - pharmacokinetics Drug Carriers - chemistry Drug Compounding - methods Gene delivery Humans Internal Medicine Lactic Acid - chemistry Lung Neoplasms - genetics Lung Neoplasms - metabolism Materials Testing Nanoparticles - chemistry Nanoparticles - ultrastructure Particle Size PLGA nanoparticles Polyglycolic Acid - chemistry Polymers - chemistry RNA, Antisense - administration & dosage RNA, Antisense - genetics RNA, Antisense - pharmacokinetics Telomerase inhibitors Transfection - methods |
| title | Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides |
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