Clonorchis sinensis: Expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-LPAP homologue

From a Clonorchis sinensis adult cDNA plasmid library, a cDNA clone encoding a novel lysophosphatidic acid phosphatase (LPAP) homologue was isolated. The predicted molecular weight of putative protein was 48.8 kDa and the deduced amino acid sequence had 45%, 32%, and 29% identity with LPAP of Schist...

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Veröffentlicht in:	Experimental parasitology 2007-10, Vol.117 (2), p.157-164
Hauptverfasser:	Hu, Fengyu, Yu, Xinbing, Ma, Changling, Zhou, Hongjuan, Zhou, Zhenwen, Li, Yanwen, Lu, Fangli, Xu, Jin, Wu, Zhongdao, Hu, Xuchu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Animals Antibodies, Helminth - blood Antigens, Helminth - biosynthesis Antigens, Helminth - chemistry Antigens, Helminth - genetics Antigens, Helminth - immunology Biological and medical sciences Blotting, Western C. sinensis Cloning, Molecular Clonorchiasis - diagnosis Clonorchiasis - immunology Clonorchis sinensis Clonorchis sinensis - enzymology Clonorchis sinensis - genetics Clonorchis sinensis - immunology complementary DNA Consensus Sequence CsLPAP Danio rerio DNA, Complementary - chemistry DNA, Helminth - chemistry Electrophoresis, Polyacrylamide Gel ELISA Enzyme-Linked Immunosorbent Assay ES antigen excretory-secretory products excretory–secretory antigen Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation, Enzymologic Humans Immune Sera - immunology immunologic techniques IPTG isopropyl-β- d-thiogalactopyranoside Life cycle. Host-agent relationship. Pathogenesis Localization LPAP LPAP of Clonorchis sinensis lysophosphatidic acid phosphatase lysophosphatidic acid phosphatase gene molecular cloning Molecular Sequence Data nucleotide sequences Phosphoric Monoester Hydrolases - biosynthesis Phosphoric Monoester Hydrolases - chemistry Phosphoric Monoester Hydrolases - genetics Phosphoric Monoester Hydrolases - immunology polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate Protozoa recombinant antigens Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - isolation & purification Schistosoma japonicum SDS–PAGE Sequence Alignment sequence homology Serodiagnosis tissue distribution Trematode
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