Altered development of neuronal progenitor cells after stimulation with autistic blood sera
Abstract Changes of brain structure and functions in people with autism may result from altered neuronal development, however, no adequate cellular or animal models are available to study neurogenesis in autism. Neuronal development can be modeled in culture of neuronal progenitor cells (NPCs) stimu...
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| Veröffentlicht in: | Brain research 2007-09, Vol.1168, p.11-20 |
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| creator | Mazur-Kolecka, Bozena Cohen, Ira L Jenkins, Edmund C Kaczmarski, Wojciech Flory, Michael Frackowiak, Janusz |
| description | Abstract Changes of brain structure and functions in people with autism may result from altered neuronal development, however, no adequate cellular or animal models are available to study neurogenesis in autism. Neuronal development can be modeled in culture of neuronal progenitor cells (NPCs) stimulated with serum to differentiate into neurons. Because sera from people with autism and age-matched controls contain different levels of numerous biologically active factors, we hypothesized that development of human NPCs induced to differentiate into neurons with sera from children with autism reflects the altered early neuronal development that leads to autism. The control and autistic sera were collected from siblings aged below 6 years that lived in the same environment. The effect of sera on differentiation of NPC neurospheres into neuronal colonies was tested in 72-h-long cultures by morphometry, immunocytochemistry and immunoblotting. We found that sera from children with autism significantly reduced NPCs' proliferation, but stimulated cell migration, development of small neurons with processes, length of processes and synaptogenesis. These results suggest that development of network of processes and synaptogenesis – the specific events in the brain during postnatal ontogenesis – are altered in autism. Further studies in this cell culture model may explain some of the cellular alterations described in autistic patients. |
| doi_str_mv | 10.1016/j.brainres.2007.06.084 |
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Neuronal development can be modeled in culture of neuronal progenitor cells (NPCs) stimulated with serum to differentiate into neurons. Because sera from people with autism and age-matched controls contain different levels of numerous biologically active factors, we hypothesized that development of human NPCs induced to differentiate into neurons with sera from children with autism reflects the altered early neuronal development that leads to autism. The control and autistic sera were collected from siblings aged below 6 years that lived in the same environment. The effect of sera on differentiation of NPC neurospheres into neuronal colonies was tested in 72-h-long cultures by morphometry, immunocytochemistry and immunoblotting. We found that sera from children with autism significantly reduced NPCs' proliferation, but stimulated cell migration, development of small neurons with processes, length of processes and synaptogenesis. These results suggest that development of network of processes and synaptogenesis – the specific events in the brain during postnatal ontogenesis – are altered in autism. Further studies in this cell culture model may explain some of the cellular alterations described in autistic patients.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2007.06.084</identifier><identifier>PMID: 17706942</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Autism ; Autistic Disorder - blood ; Biological and medical sciences ; Bromodeoxyuridine - metabolism ; Cell Count - methods ; Cell culture ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Proliferation - drug effects ; Cell Size - drug effects ; Cells, Cultured ; Cerebral Cortex - cytology ; Child clinical studies ; Child, Preschool ; Developmental disorders ; Electrophoresis, Capillary - methods ; Female ; Fetus ; Humans ; Infant ; Infantile autism ; Male ; Medical sciences ; Morphometry ; Nerve Tissue Proteins - metabolism ; Neurogenesis ; Neurology ; Neuronal progenitor cell ; Neurons - drug effects ; Psychology. Psychoanalysis. Psychiatry ; Psychopathology. Psychiatry ; Serum ; Serum Globulins - pharmacology ; Stem Cells - drug effects ; Stem Cells - physiology</subject><ispartof>Brain research, 2007-09, Vol.1168, p.11-20</ispartof><rights>Elsevier B.V.</rights><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-25557985eacf9e17cb461b68c4013c955d9e8996593cad95b27b0a0d1661b8223</citedby><cites>FETCH-LOGICAL-c482t-25557985eacf9e17cb461b68c4013c955d9e8996593cad95b27b0a0d1661b8223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2007.06.084$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19061972$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17706942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mazur-Kolecka, Bozena</creatorcontrib><creatorcontrib>Cohen, Ira L</creatorcontrib><creatorcontrib>Jenkins, Edmund C</creatorcontrib><creatorcontrib>Kaczmarski, Wojciech</creatorcontrib><creatorcontrib>Flory, Michael</creatorcontrib><creatorcontrib>Frackowiak, Janusz</creatorcontrib><title>Altered development of neuronal progenitor cells after stimulation with autistic blood sera</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Abstract Changes of brain structure and functions in people with autism may result from altered neuronal development, however, no adequate cellular or animal models are available to study neurogenesis in autism. Neuronal development can be modeled in culture of neuronal progenitor cells (NPCs) stimulated with serum to differentiate into neurons. Because sera from people with autism and age-matched controls contain different levels of numerous biologically active factors, we hypothesized that development of human NPCs induced to differentiate into neurons with sera from children with autism reflects the altered early neuronal development that leads to autism. The control and autistic sera were collected from siblings aged below 6 years that lived in the same environment. The effect of sera on differentiation of NPC neurospheres into neuronal colonies was tested in 72-h-long cultures by morphometry, immunocytochemistry and immunoblotting. We found that sera from children with autism significantly reduced NPCs' proliferation, but stimulated cell migration, development of small neurons with processes, length of processes and synaptogenesis. These results suggest that development of network of processes and synaptogenesis – the specific events in the brain during postnatal ontogenesis – are altered in autism. Further studies in this cell culture model may explain some of the cellular alterations described in autistic patients.</description><subject>Autism</subject><subject>Autistic Disorder - blood</subject><subject>Biological and medical sciences</subject><subject>Bromodeoxyuridine - metabolism</subject><subject>Cell Count - methods</subject><subject>Cell culture</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Size - drug effects</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - cytology</subject><subject>Child clinical studies</subject><subject>Child, Preschool</subject><subject>Developmental disorders</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Female</subject><subject>Fetus</subject><subject>Humans</subject><subject>Infant</subject><subject>Infantile autism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Morphometry</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurogenesis</subject><subject>Neurology</subject><subject>Neuronal progenitor cell</subject><subject>Neurons - drug effects</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopathology. Psychiatry</subject><subject>Serum</subject><subject>Serum Globulins - pharmacology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - physiology</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk-L1TAUxYsoznP0KwzZ6K71Jm2TZiMOw_gHBlyoILgIaXqreabJM0lH5tub8p4MuJlVSPide889uVV1QaGhQPnrfTNGbX3E1DAA0QBvYOgeVTs6CFZz1sHjagcAvB6kbM-qZynty7VtJTytzqgQwGXHdtX3S5cx4kQmvEUXDgv6TMJMPK4xeO3IIYYf6G0OkRh0LhE9FwFJ2S6r09kGT_7Y_JPoNdvyaMjoQphIwqifV09m7RK-OJ3n1dd311-uPtQ3n95_vLq8qU03sFyzvu-FHHrUZpZIhRk7Tkc-mA5oa2TfTxLLELyXrdGT7EcmRtAwUV6wgbH2vHp1rFu8_l4xZbXYtJnVHsOaFC9QwfoHQSq47DmDAvIjaGJIKeKsDtEuOt4pCmrLX-3Vv_zVlr8Crkr-RXhx6rCOC073slPgBXh5AnQy2s1Re2PTPSeBUyk27u2RwxLcrcWokrHoDU42oslqCvZhL2_-K2Gc9bZ0_YV3mPZhjeWDy9QqMQXq87Yt27KAANqx4Vv7F12xvLQ</recordid><startdate>20070907</startdate><enddate>20070907</enddate><creator>Mazur-Kolecka, Bozena</creator><creator>Cohen, Ira L</creator><creator>Jenkins, Edmund C</creator><creator>Kaczmarski, Wojciech</creator><creator>Flory, Michael</creator><creator>Frackowiak, Janusz</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20070907</creationdate><title>Altered development of neuronal progenitor cells after stimulation with autistic blood sera</title><author>Mazur-Kolecka, Bozena ; Cohen, Ira L ; Jenkins, Edmund C ; Kaczmarski, Wojciech ; Flory, Michael ; Frackowiak, Janusz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-25557985eacf9e17cb461b68c4013c955d9e8996593cad95b27b0a0d1661b8223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Autism</topic><topic>Autistic Disorder - blood</topic><topic>Biological and medical sciences</topic><topic>Bromodeoxyuridine - metabolism</topic><topic>Cell Count - methods</topic><topic>Cell culture</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Size - drug effects</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - cytology</topic><topic>Child clinical studies</topic><topic>Child, Preschool</topic><topic>Developmental disorders</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Female</topic><topic>Fetus</topic><topic>Humans</topic><topic>Infant</topic><topic>Infantile autism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Morphometry</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurogenesis</topic><topic>Neurology</topic><topic>Neuronal progenitor cell</topic><topic>Neurons - drug effects</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopathology. Psychiatry</topic><topic>Serum</topic><topic>Serum Globulins - pharmacology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mazur-Kolecka, Bozena</creatorcontrib><creatorcontrib>Cohen, Ira L</creatorcontrib><creatorcontrib>Jenkins, Edmund C</creatorcontrib><creatorcontrib>Kaczmarski, Wojciech</creatorcontrib><creatorcontrib>Flory, Michael</creatorcontrib><creatorcontrib>Frackowiak, Janusz</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mazur-Kolecka, Bozena</au><au>Cohen, Ira L</au><au>Jenkins, Edmund C</au><au>Kaczmarski, Wojciech</au><au>Flory, Michael</au><au>Frackowiak, Janusz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered development of neuronal progenitor cells after stimulation with autistic blood sera</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2007-09-07</date><risdate>2007</risdate><volume>1168</volume><spage>11</spage><epage>20</epage><pages>11-20</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Abstract Changes of brain structure and functions in people with autism may result from altered neuronal development, however, no adequate cellular or animal models are available to study neurogenesis in autism. 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| subjects | Autism Autistic Disorder - blood Biological and medical sciences Bromodeoxyuridine - metabolism Cell Count - methods Cell culture Cell Differentiation - drug effects Cell Differentiation - physiology Cell Proliferation - drug effects Cell Size - drug effects Cells, Cultured Cerebral Cortex - cytology Child clinical studies Child, Preschool Developmental disorders Electrophoresis, Capillary - methods Female Fetus Humans Infant Infantile autism Male Medical sciences Morphometry Nerve Tissue Proteins - metabolism Neurogenesis Neurology Neuronal progenitor cell Neurons - drug effects Psychology. Psychoanalysis. Psychiatry Psychopathology. Psychiatry Serum Serum Globulins - pharmacology Stem Cells - drug effects Stem Cells - physiology |
| title | Altered development of neuronal progenitor cells after stimulation with autistic blood sera |
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