Enhanced Fc-Dependent Cellular Cytotoxicity of Fc Fusion Proteins Derived from TNF Receptor II and LFA-3 by Fucose Removal from Asn-Linked Oligosaccharides

Fucose removal from complex-type oligosaccharide of human IgGs results in a major enhancement of Fc-dependent cellular cytotoxicity. The aim of this study was to determine the effect of fucose removal on the effector function of another class of clinically important molecules that can effect cellula...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 2006-12, Vol.140 (6), p.777-783
Hauptverfasser:	Shoji-Hosaka, Emi, Kobayashi, Yukari, Wakitani, Masako, Uchida, Kazuhisa, Niwa, Rinpei, Nakamura, Kazuyasu, Shitara, Kenya
Format:	Artikel
Sprache:	eng
Schlagworte:	ADCC Animals Antibody-Dependent Cell Cytotoxicity - drug effects antibody-dependent cellular cytotoxicity CD58 Antigens - immunology CDC Chinese hamster ovary CHO CHO Cells complement-dependent cytotoxicity Cricetinae Cricetulus cytotoxicity defucosylation ELISA enzyme-linked immunosorbent assay Etanercept Fc fusion FcγRIIIa Fucose - chemistry high-performance anion exchange chromatography HPAC Humans immunoglobulin Immunoglobulin Fc Fragments - immunology Immunoglobulin G - immunology lactate dehydrogenase LDH leukocyte function antigen-3 LFA-3 means of fluorescence intensity MFI natural killer oligosaccharide Oligosaccharides - chemistry Oligosaccharides - immunology PBMC PBS peripheral blood mononuclear cell phosphate buffered saline Receptors, IgG - immunology Receptors, Tumor Necrosis Factor - immunology Recombinant Fusion Proteins - immunology TNFRII tumor necrosis factor receptor II
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