Utility of the polymerase chain reaction in the diagnosis of tuberculous meningitis
Due to inconsistent clinical presentations and the lack of a rapid, sensitive and specific test, tuberculous meningitis (TBM) is particularly difficult to diagnose. The present study was carried out to determine the utility of the polymerase chain reaction (PCR) using INS primers in the diagnosis of...
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| Veröffentlicht in: | Research in microbiology 2006-12, Vol.157 (10), p.967-970 |
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| creator | Desai, Dhyanesh Nataraj, Gita Kulkarni, Savita Bichile, Lata Mehta, Preeti Baveja, Sujata Rajan, Ramakrishna Raut, Abhijit Shenoy, Asha |
| description | Due to inconsistent clinical presentations and the lack of a rapid, sensitive and specific test, tuberculous meningitis (TBM) is particularly difficult to diagnose. The present study was carried out to determine the utility of the polymerase chain reaction (PCR) using INS primers in the diagnosis of TBM and to compare the efficacy of two different DNA extraction protocols. Fifty-seven cerebrospinal fluid (CSF) samples from suspected cases of meningitis—30 definitive/possible TBM and 27 non-TBM—were processed for microscopy, culture and PCR. Results of computer tomographic (CT) scan findings were noted. The results of smear, culture and PCR were compared using culture and/or clinical response to treatment as the gold standard. The sensitivity of microscopy, culture, CT scan and PCR was 3.3%, 26.7%, 60.0% and 66.7%, respectively. PCR following QIAmp DNA extraction had a sensitivity of 66.7% compared to PCR following a DNA extraction protocol based on the use of cetyl trimethyl ammonium bromide (CTAB) (50%). PCR was positive in all culture-positive CSF samples using either extraction method. PCR is a rapid and sensitive technique; above all, it can diagnose tuberculous meningitis at a very early stage. |
| doi_str_mv | 10.1016/j.resmic.2006.08.002 |
| format | Article |
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The present study was carried out to determine the utility of the polymerase chain reaction (PCR) using INS primers in the diagnosis of TBM and to compare the efficacy of two different DNA extraction protocols. Fifty-seven cerebrospinal fluid (CSF) samples from suspected cases of meningitis—30 definitive/possible TBM and 27 non-TBM—were processed for microscopy, culture and PCR. Results of computer tomographic (CT) scan findings were noted. The results of smear, culture and PCR were compared using culture and/or clinical response to treatment as the gold standard. The sensitivity of microscopy, culture, CT scan and PCR was 3.3%, 26.7%, 60.0% and 66.7%, respectively. PCR following QIAmp DNA extraction had a sensitivity of 66.7% compared to PCR following a DNA extraction protocol based on the use of cetyl trimethyl ammonium bromide (CTAB) (50%). PCR was positive in all culture-positive CSF samples using either extraction method. PCR is a rapid and sensitive technique; above all, it can diagnose tuberculous meningitis at a very early stage.</description><identifier>ISSN: 0923-2508</identifier><identifier>EISSN: 1769-7123</identifier><identifier>DOI: 10.1016/j.resmic.2006.08.002</identifier><identifier>PMID: 17008064</identifier><language>eng</language><publisher>Paris: Elsevier SAS</publisher><subject>Biological and medical sciences ; CT scan ; Diagnosis ; DNA extraction ; Fundamental and applied biological sciences. Psychology ; Humans ; Microbiology ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Prospective Studies ; Sensitivity and Specificity ; Tomography, X-Ray Computed ; Tuberculosis, Meningeal - cerebrospinal fluid ; Tuberculosis, Meningeal - diagnosis ; Tuberculosis, Meningeal - microbiology ; Tuberculous meningitis</subject><ispartof>Research in microbiology, 2006-12, Vol.157 (10), p.967-970</ispartof><rights>2006 Elsevier SAS</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-1e434800eee72dbae71e28d3a28fe6588448b2ac5262a45924c6f1b11f33d2e93</citedby><cites>FETCH-LOGICAL-c467t-1e434800eee72dbae71e28d3a28fe6588448b2ac5262a45924c6f1b11f33d2e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.resmic.2006.08.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27928,27929,45999</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18348790$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17008064$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Desai, Dhyanesh</creatorcontrib><creatorcontrib>Nataraj, Gita</creatorcontrib><creatorcontrib>Kulkarni, Savita</creatorcontrib><creatorcontrib>Bichile, Lata</creatorcontrib><creatorcontrib>Mehta, Preeti</creatorcontrib><creatorcontrib>Baveja, Sujata</creatorcontrib><creatorcontrib>Rajan, Ramakrishna</creatorcontrib><creatorcontrib>Raut, Abhijit</creatorcontrib><creatorcontrib>Shenoy, Asha</creatorcontrib><title>Utility of the polymerase chain reaction in the diagnosis of tuberculous meningitis</title><title>Research in microbiology</title><addtitle>Res Microbiol</addtitle><description>Due to inconsistent clinical presentations and the lack of a rapid, sensitive and specific test, tuberculous meningitis (TBM) is particularly difficult to diagnose. The present study was carried out to determine the utility of the polymerase chain reaction (PCR) using INS primers in the diagnosis of TBM and to compare the efficacy of two different DNA extraction protocols. Fifty-seven cerebrospinal fluid (CSF) samples from suspected cases of meningitis—30 definitive/possible TBM and 27 non-TBM—were processed for microscopy, culture and PCR. Results of computer tomographic (CT) scan findings were noted. The results of smear, culture and PCR were compared using culture and/or clinical response to treatment as the gold standard. The sensitivity of microscopy, culture, CT scan and PCR was 3.3%, 26.7%, 60.0% and 66.7%, respectively. PCR following QIAmp DNA extraction had a sensitivity of 66.7% compared to PCR following a DNA extraction protocol based on the use of cetyl trimethyl ammonium bromide (CTAB) (50%). PCR was positive in all culture-positive CSF samples using either extraction method. PCR is a rapid and sensitive technique; above all, it can diagnose tuberculous meningitis at a very early stage.</description><subject>Biological and medical sciences</subject><subject>CT scan</subject><subject>Diagnosis</subject><subject>DNA extraction</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Prospective Studies</subject><subject>Sensitivity and Specificity</subject><subject>Tomography, X-Ray Computed</subject><subject>Tuberculosis, Meningeal - cerebrospinal fluid</subject><subject>Tuberculosis, Meningeal - diagnosis</subject><subject>Tuberculosis, Meningeal - microbiology</subject><subject>Tuberculous meningitis</subject><issn>0923-2508</issn><issn>1769-7123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3DAUhUVoSaZJ_0Ep3jQ7O1cPS_ImUELSFgJdNFkLWb5O7uDHRLID8-9jdwaya1f3Lr5zOHyMfeFQcOD6altETD2FQgDoAmwBIE7Yhhtd5YYL-YFtoBIyFyXYM_YppS0AL41Rp-yMGwALWm3Yn8eJOpr22dhm0zNmu7Hb9xh9wiw8exqyiD5MNA7Z8q9AQ_5pGBOlv4m5xhjmbpxT1uNAwxNNlC7Yx9Z3CT8f7zl7vLt9uPmZ3__-8evm-30elDZTzlFJZQEQ0Yim9mg4CttIL2yLurRWKVsLH0qhhVdlJVTQLa85b6VsBFbynF0eendxfJkxTa6nFLDr_IDLIqet4NZw-C_IK1kZK9dGdQBDHFOK2LpdpN7HvePgVutu6w7W3WrdgXWL9SX29dg_1z0276Gj5gX4dgR8Cr5rox8CpXfOLiJMtQ69PnC4aHsljC4FwiFgQxHD5JqR_r3kDTImomM</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Desai, Dhyanesh</creator><creator>Nataraj, Gita</creator><creator>Kulkarni, Savita</creator><creator>Bichile, Lata</creator><creator>Mehta, Preeti</creator><creator>Baveja, Sujata</creator><creator>Rajan, Ramakrishna</creator><creator>Raut, Abhijit</creator><creator>Shenoy, Asha</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Utility of the polymerase chain reaction in the diagnosis of tuberculous meningitis</title><author>Desai, Dhyanesh ; Nataraj, Gita ; Kulkarni, Savita ; Bichile, Lata ; Mehta, Preeti ; Baveja, Sujata ; Rajan, Ramakrishna ; Raut, Abhijit ; Shenoy, Asha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-1e434800eee72dbae71e28d3a28fe6588448b2ac5262a45924c6f1b11f33d2e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>CT scan</topic><topic>Diagnosis</topic><topic>DNA extraction</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Prospective Studies</topic><topic>Sensitivity and Specificity</topic><topic>Tomography, X-Ray Computed</topic><topic>Tuberculosis, Meningeal - cerebrospinal fluid</topic><topic>Tuberculosis, Meningeal - diagnosis</topic><topic>Tuberculosis, Meningeal - microbiology</topic><topic>Tuberculous meningitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Desai, Dhyanesh</creatorcontrib><creatorcontrib>Nataraj, Gita</creatorcontrib><creatorcontrib>Kulkarni, Savita</creatorcontrib><creatorcontrib>Bichile, Lata</creatorcontrib><creatorcontrib>Mehta, Preeti</creatorcontrib><creatorcontrib>Baveja, Sujata</creatorcontrib><creatorcontrib>Rajan, Ramakrishna</creatorcontrib><creatorcontrib>Raut, Abhijit</creatorcontrib><creatorcontrib>Shenoy, Asha</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Research in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Desai, Dhyanesh</au><au>Nataraj, Gita</au><au>Kulkarni, Savita</au><au>Bichile, Lata</au><au>Mehta, Preeti</au><au>Baveja, Sujata</au><au>Rajan, Ramakrishna</au><au>Raut, Abhijit</au><au>Shenoy, Asha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utility of the polymerase chain reaction in the diagnosis of tuberculous meningitis</atitle><jtitle>Research in microbiology</jtitle><addtitle>Res Microbiol</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>157</volume><issue>10</issue><spage>967</spage><epage>970</epage><pages>967-970</pages><issn>0923-2508</issn><eissn>1769-7123</eissn><abstract>Due to inconsistent clinical presentations and the lack of a rapid, sensitive and specific test, tuberculous meningitis (TBM) is particularly difficult to diagnose. The present study was carried out to determine the utility of the polymerase chain reaction (PCR) using INS primers in the diagnosis of TBM and to compare the efficacy of two different DNA extraction protocols. Fifty-seven cerebrospinal fluid (CSF) samples from suspected cases of meningitis—30 definitive/possible TBM and 27 non-TBM—were processed for microscopy, culture and PCR. Results of computer tomographic (CT) scan findings were noted. The results of smear, culture and PCR were compared using culture and/or clinical response to treatment as the gold standard. The sensitivity of microscopy, culture, CT scan and PCR was 3.3%, 26.7%, 60.0% and 66.7%, respectively. PCR following QIAmp DNA extraction had a sensitivity of 66.7% compared to PCR following a DNA extraction protocol based on the use of cetyl trimethyl ammonium bromide (CTAB) (50%). PCR was positive in all culture-positive CSF samples using either extraction method. 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| ispartof | Research in microbiology, 2006-12, Vol.157 (10), p.967-970 |
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| subjects | Biological and medical sciences CT scan Diagnosis DNA extraction Fundamental and applied biological sciences. Psychology Humans Microbiology Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Polymerase chain reaction Polymerase Chain Reaction - methods Prospective Studies Sensitivity and Specificity Tomography, X-Ray Computed Tuberculosis, Meningeal - cerebrospinal fluid Tuberculosis, Meningeal - diagnosis Tuberculosis, Meningeal - microbiology Tuberculous meningitis |
| title | Utility of the polymerase chain reaction in the diagnosis of tuberculous meningitis |
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