Distribution and Characteristics of Listeria monocytogenes Isolates from Surface Waters of the South Nation River Watershed, Ontario, Canada
Listeria monocytogenes is a facultative intracellular pathogen thought to be widely distributed in the environment. We investigated the prevalence and characteristics of L. monocytogenes isolates from surface waters derived from catchments within the South Nation River watershed (Ontario, Canada). T...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2007-09, Vol.73 (17), p.5401-5410 |
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| creator | Lyautey, Emilie Lapen, David R Wilkes, Graham McCleary, Katherine Pagotto, Franco Tyler, Kevin Hartmann, Alain Piveteau, Pascal Rieu, Aurélie Robertson, William J Medeiros, Diane T Edge, Thomas A Gannon, Victor Topp, Edward |
| description | Listeria monocytogenes is a facultative intracellular pathogen thought to be widely distributed in the environment. We investigated the prevalence and characteristics of L. monocytogenes isolates from surface waters derived from catchments within the South Nation River watershed (Ontario, Canada). This watershed is dominated by urban and rural development, livestock and crop production, and wildlife habitats. From June to November 2005, a total of 314 surface water samples were collected biweekly from 22 discrete sampling sites characterized by various upstream land uses. Presumptive Listeria spp. were isolated using a selective enrichment and isolation procedure, and 75 L. monocytogenes isolates were identified based on colony morphology, hemolytic activity, and amplification of three pathogenicity genes: iap, inlA, and hlyA. Thirty-two of 314 (10%) surface water samples were positive for the presence of L. monocytogenes, but detection ranged between 0 and 27% depending on the sampling date. Isolates belonging to serovar group 1/2a, 3a (50%) and group 4b, 4d, 4e (32%) were dominant. L. monocytogenes populations were resolved into 13 EcoRI ribotypes and 21 ApaI and 21 AscI pulsotypes. These had Simpson indexes of discrimination of up to 0.885. Lineage I-related isolates were dominant (61%) during the summer, whereas lineage II isolates were dominant (77%) in the fall. Isolates were, on average, resistant to 6.1 ± 2.1 antibiotics out of 17 tested. Half of the L. monocytogenes isolates exhibited potential virulence linked to the production of a functional internalin A, and some isolates were found to be moderately to highly virulent by in vitro Caco-2 plaque formation assay (up to 28% of entry). There was a statistically significant link between the occurrence of L. monocytogenes and proximity to an upstream dairy farm and degree of cropped land. Our data indicate that L. monocytogenes is widespread in the studied catchments, where it could represent a public health issue related to agricultural land use. |
| doi_str_mv | 10.1128/AEM.00354-07 |
| format | Article |
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We investigated the prevalence and characteristics of L. monocytogenes isolates from surface waters derived from catchments within the South Nation River watershed (Ontario, Canada). This watershed is dominated by urban and rural development, livestock and crop production, and wildlife habitats. From June to November 2005, a total of 314 surface water samples were collected biweekly from 22 discrete sampling sites characterized by various upstream land uses. Presumptive Listeria spp. were isolated using a selective enrichment and isolation procedure, and 75 L. monocytogenes isolates were identified based on colony morphology, hemolytic activity, and amplification of three pathogenicity genes: iap, inlA, and hlyA. Thirty-two of 314 (10%) surface water samples were positive for the presence of L. monocytogenes, but detection ranged between 0 and 27% depending on the sampling date. Isolates belonging to serovar group 1/2a, 3a (50%) and group 4b, 4d, 4e (32%) were dominant. L. monocytogenes populations were resolved into 13 EcoRI ribotypes and 21 ApaI and 21 AscI pulsotypes. These had Simpson indexes of discrimination of up to 0.885. Lineage I-related isolates were dominant (61%) during the summer, whereas lineage II isolates were dominant (77%) in the fall. Isolates were, on average, resistant to 6.1 ± 2.1 antibiotics out of 17 tested. Half of the L. monocytogenes isolates exhibited potential virulence linked to the production of a functional internalin A, and some isolates were found to be moderately to highly virulent by in vitro Caco-2 plaque formation assay (up to 28% of entry). There was a statistically significant link between the occurrence of L. monocytogenes and proximity to an upstream dairy farm and degree of cropped land. Our data indicate that L. monocytogenes is widespread in the studied catchments, where it could represent a public health issue related to agricultural land use.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.00354-07</identifier><identifier>PMID: 17630309</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Agriculture ; Animals ; Anti-Bacterial Agents - pharmacology ; Bacteria ; Bacterial Typing Techniques ; Biological and medical sciences ; Culture Media ; Ecology, environment ; Ecosystem ; Environmental impact ; Environmental Microbiology ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Genotype ; Humans ; Life Sciences ; Listeria monocytogenes ; Listeria monocytogenes - classification ; Listeria monocytogenes - genetics ; Listeria monocytogenes - isolation & purification ; Listeria monocytogenes - pathogenicity ; Microbial Sensitivity Tests ; Microbiology ; Ontario ; Phenotype ; Rivers ; Rivers - microbiology ; Seasons ; Social Planning ; Surface water ; Urban Renewal ; Virulence - genetics ; Watersheds</subject><ispartof>Applied and Environmental Microbiology, 2007-09, Vol.73 (17), p.5401-5410</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Sep 2007</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2007, American Society for Microbiology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c598t-bd77c0452d2626573ee15e165ddeac6940ebd4200429236272874d8378fb935b3</citedby><cites>FETCH-LOGICAL-c598t-bd77c0452d2626573ee15e165ddeac6940ebd4200429236272874d8378fb935b3</cites><orcidid>0000-0002-1210-251X ; 0000-0003-0754-197X ; 0000-0001-6034-5068</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2042075/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2042075/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19050347$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17630309$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02667136$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Lyautey, Emilie</creatorcontrib><creatorcontrib>Lapen, David R</creatorcontrib><creatorcontrib>Wilkes, Graham</creatorcontrib><creatorcontrib>McCleary, Katherine</creatorcontrib><creatorcontrib>Pagotto, Franco</creatorcontrib><creatorcontrib>Tyler, Kevin</creatorcontrib><creatorcontrib>Hartmann, Alain</creatorcontrib><creatorcontrib>Piveteau, Pascal</creatorcontrib><creatorcontrib>Rieu, Aurélie</creatorcontrib><creatorcontrib>Robertson, William J</creatorcontrib><creatorcontrib>Medeiros, Diane T</creatorcontrib><creatorcontrib>Edge, Thomas A</creatorcontrib><creatorcontrib>Gannon, Victor</creatorcontrib><creatorcontrib>Topp, Edward</creatorcontrib><title>Distribution and Characteristics of Listeria monocytogenes Isolates from Surface Waters of the South Nation River Watershed, Ontario, Canada</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Listeria monocytogenes is a facultative intracellular pathogen thought to be widely distributed in the environment. We investigated the prevalence and characteristics of L. monocytogenes isolates from surface waters derived from catchments within the South Nation River watershed (Ontario, Canada). This watershed is dominated by urban and rural development, livestock and crop production, and wildlife habitats. From June to November 2005, a total of 314 surface water samples were collected biweekly from 22 discrete sampling sites characterized by various upstream land uses. Presumptive Listeria spp. were isolated using a selective enrichment and isolation procedure, and 75 L. monocytogenes isolates were identified based on colony morphology, hemolytic activity, and amplification of three pathogenicity genes: iap, inlA, and hlyA. Thirty-two of 314 (10%) surface water samples were positive for the presence of L. monocytogenes, but detection ranged between 0 and 27% depending on the sampling date. Isolates belonging to serovar group 1/2a, 3a (50%) and group 4b, 4d, 4e (32%) were dominant. L. monocytogenes populations were resolved into 13 EcoRI ribotypes and 21 ApaI and 21 AscI pulsotypes. These had Simpson indexes of discrimination of up to 0.885. Lineage I-related isolates were dominant (61%) during the summer, whereas lineage II isolates were dominant (77%) in the fall. Isolates were, on average, resistant to 6.1 ± 2.1 antibiotics out of 17 tested. Half of the L. monocytogenes isolates exhibited potential virulence linked to the production of a functional internalin A, and some isolates were found to be moderately to highly virulent by in vitro Caco-2 plaque formation assay (up to 28% of entry). There was a statistically significant link between the occurrence of L. monocytogenes and proximity to an upstream dairy farm and degree of cropped land. Our data indicate that L. monocytogenes is widespread in the studied catchments, where it could represent a public health issue related to agricultural land use.</description><subject>Agriculture</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteria</subject><subject>Bacterial Typing Techniques</subject><subject>Biological and medical sciences</subject><subject>Culture Media</subject><subject>Ecology, environment</subject><subject>Ecosystem</subject><subject>Environmental impact</subject><subject>Environmental Microbiology</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - classification</subject><subject>Listeria monocytogenes - genetics</subject><subject>Listeria monocytogenes - isolation & purification</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Ontario</subject><subject>Phenotype</subject><subject>Rivers</subject><subject>Rivers - microbiology</subject><subject>Seasons</subject><subject>Social Planning</subject><subject>Surface water</subject><subject>Urban Renewal</subject><subject>Virulence - genetics</subject><subject>Watersheds</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhiMEomXhxhksJJCQNmVsx3Z8QVothVZaqMRScbQcx9m42sTFThb1P_Cj8X6IhV44-WOeeWc8frPsOYYzjEn5bnb--QyAsiIH8SA7xSDLnFHKH2anAFLmhBRwkj2J8QYACuDl4-wEC06BgjzNfn1wcQiuGgfne6T7Gs1bHbQZbEgBZyLyDVqkbTpr1Pnem7vBr2xvI7qMfq2HtGmC79ByDI02Fn1PV2GXNrQWLf04tOiL3sl_dRsbDkBr6ym66gcdnJ-iue51rZ9mjxq9jvbZYZ1k1x_Pv80v8sXVp8v5bJEbJsshr2ohDBSM1IQTzgS1FjOLOatrqw2XBdiqLkh6LZGEciJIKYq6pKJsKklZRSfZ-73u7Vh1tja2H4Jeq9vgOh3ulNdO_RvpXatWfqNIkgTBksDbvUB7L-1itlDbOyCcC0z5Bif2zaFY8D9GGwfVuWjseq1768eoeEkww0L-FyTAQfD01ZPs1T3wxo-hTxNLDJNcSLwtO91DJvgYg23-9IlBbY2jknHUzjgKRMJf_D2SI3xwSgJeHwAdjV43QffGxSMngQEtxLG51q3any5YpWOntO2UoElNsQK2zb3cQ432Sq-S1dT1kqQAQAkEqKS_AUHk32Q</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Lyautey, Emilie</creator><creator>Lapen, David R</creator><creator>Wilkes, Graham</creator><creator>McCleary, Katherine</creator><creator>Pagotto, Franco</creator><creator>Tyler, Kevin</creator><creator>Hartmann, Alain</creator><creator>Piveteau, Pascal</creator><creator>Rieu, Aurélie</creator><creator>Robertson, William J</creator><creator>Medeiros, Diane T</creator><creator>Edge, Thomas A</creator><creator>Gannon, Victor</creator><creator>Topp, Edward</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7QH</scope><scope>7TV</scope><scope>7UA</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1210-251X</orcidid><orcidid>https://orcid.org/0000-0003-0754-197X</orcidid><orcidid>https://orcid.org/0000-0001-6034-5068</orcidid></search><sort><creationdate>20070901</creationdate><title>Distribution and Characteristics of Listeria monocytogenes Isolates from Surface Waters of the South Nation River Watershed, Ontario, Canada</title><author>Lyautey, Emilie ; Lapen, David R ; Wilkes, Graham ; McCleary, Katherine ; Pagotto, Franco ; Tyler, Kevin ; Hartmann, Alain ; Piveteau, Pascal ; Rieu, Aurélie ; Robertson, William J ; Medeiros, Diane T ; Edge, Thomas A ; Gannon, Victor ; Topp, Edward</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c598t-bd77c0452d2626573ee15e165ddeac6940ebd4200429236272874d8378fb935b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agriculture</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteria</topic><topic>Bacterial Typing Techniques</topic><topic>Biological and medical sciences</topic><topic>Culture Media</topic><topic>Ecology, environment</topic><topic>Ecosystem</topic><topic>Environmental impact</topic><topic>Environmental Microbiology</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - classification</topic><topic>Listeria monocytogenes - genetics</topic><topic>Listeria monocytogenes - isolation & purification</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Ontario</topic><topic>Phenotype</topic><topic>Rivers</topic><topic>Rivers - microbiology</topic><topic>Seasons</topic><topic>Social Planning</topic><topic>Surface water</topic><topic>Urban Renewal</topic><topic>Virulence - genetics</topic><topic>Watersheds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lyautey, Emilie</creatorcontrib><creatorcontrib>Lapen, David R</creatorcontrib><creatorcontrib>Wilkes, Graham</creatorcontrib><creatorcontrib>McCleary, Katherine</creatorcontrib><creatorcontrib>Pagotto, Franco</creatorcontrib><creatorcontrib>Tyler, Kevin</creatorcontrib><creatorcontrib>Hartmann, Alain</creatorcontrib><creatorcontrib>Piveteau, Pascal</creatorcontrib><creatorcontrib>Rieu, Aurélie</creatorcontrib><creatorcontrib>Robertson, William J</creatorcontrib><creatorcontrib>Medeiros, Diane T</creatorcontrib><creatorcontrib>Edge, Thomas A</creatorcontrib><creatorcontrib>Gannon, Victor</creatorcontrib><creatorcontrib>Topp, Edward</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lyautey, Emilie</au><au>Lapen, David R</au><au>Wilkes, Graham</au><au>McCleary, Katherine</au><au>Pagotto, Franco</au><au>Tyler, Kevin</au><au>Hartmann, Alain</au><au>Piveteau, Pascal</au><au>Rieu, Aurélie</au><au>Robertson, William J</au><au>Medeiros, Diane T</au><au>Edge, Thomas A</au><au>Gannon, Victor</au><au>Topp, Edward</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution and Characteristics of Listeria monocytogenes Isolates from Surface Waters of the South Nation River Watershed, Ontario, Canada</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>73</volume><issue>17</issue><spage>5401</spage><epage>5410</epage><pages>5401-5410</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Listeria monocytogenes is a facultative intracellular pathogen thought to be widely distributed in the environment. We investigated the prevalence and characteristics of L. monocytogenes isolates from surface waters derived from catchments within the South Nation River watershed (Ontario, Canada). This watershed is dominated by urban and rural development, livestock and crop production, and wildlife habitats. From June to November 2005, a total of 314 surface water samples were collected biweekly from 22 discrete sampling sites characterized by various upstream land uses. Presumptive Listeria spp. were isolated using a selective enrichment and isolation procedure, and 75 L. monocytogenes isolates were identified based on colony morphology, hemolytic activity, and amplification of three pathogenicity genes: iap, inlA, and hlyA. Thirty-two of 314 (10%) surface water samples were positive for the presence of L. monocytogenes, but detection ranged between 0 and 27% depending on the sampling date. Isolates belonging to serovar group 1/2a, 3a (50%) and group 4b, 4d, 4e (32%) were dominant. L. monocytogenes populations were resolved into 13 EcoRI ribotypes and 21 ApaI and 21 AscI pulsotypes. These had Simpson indexes of discrimination of up to 0.885. Lineage I-related isolates were dominant (61%) during the summer, whereas lineage II isolates were dominant (77%) in the fall. Isolates were, on average, resistant to 6.1 ± 2.1 antibiotics out of 17 tested. Half of the L. monocytogenes isolates exhibited potential virulence linked to the production of a functional internalin A, and some isolates were found to be moderately to highly virulent by in vitro Caco-2 plaque formation assay (up to 28% of entry). There was a statistically significant link between the occurrence of L. monocytogenes and proximity to an upstream dairy farm and degree of cropped land. Our data indicate that L. monocytogenes is widespread in the studied catchments, where it could represent a public health issue related to agricultural land use.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>17630309</pmid><doi>10.1128/AEM.00354-07</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-1210-251X</orcidid><orcidid>https://orcid.org/0000-0003-0754-197X</orcidid><orcidid>https://orcid.org/0000-0001-6034-5068</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 2007-09, Vol.73 (17), p.5401-5410 |
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| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Agriculture Animals Anti-Bacterial Agents - pharmacology Bacteria Bacterial Typing Techniques Biological and medical sciences Culture Media Ecology, environment Ecosystem Environmental impact Environmental Microbiology Freshwater Fundamental and applied biological sciences. Psychology Genotype Humans Life Sciences Listeria monocytogenes Listeria monocytogenes - classification Listeria monocytogenes - genetics Listeria monocytogenes - isolation & purification Listeria monocytogenes - pathogenicity Microbial Sensitivity Tests Microbiology Ontario Phenotype Rivers Rivers - microbiology Seasons Social Planning Surface water Urban Renewal Virulence - genetics Watersheds |
| title | Distribution and Characteristics of Listeria monocytogenes Isolates from Surface Waters of the South Nation River Watershed, Ontario, Canada |
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