Tissue Reactions of Adenoviral, Adeno-Associated Viral, and Liposome–Plasmid Vectors in Tendons and Comparison With Early-Stage Healing Responses of Injured Flexor Tendons
Delivery of growth factor genes that may substantially increase the healing rate of injured digital flexor tendons is a new application of gene therapy. Adenoviral, adeno-associated viral (AAV), and liposome–plasmid vectors have been used to deliver genes to tendons, but the tendon reactions to thes...
Gespeichert in:
| Veröffentlicht in: | The Journal of hand surgery (American ed.) 2006-12, Vol.31 (10), p.1652-1660 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1660 |
|---|---|
| container_issue | 10 |
| container_start_page | 1652 |
| container_title | The Journal of hand surgery (American ed.) |
| container_volume | 31 |
| creator | Zhu, Bei Cao, Yi Xin, Ke-Qin Wang, Xiao Tian Summerhayes, Ian C. Liu, Paul Y. Tang, Jin Bo |
| description | Delivery of growth factor genes that may substantially increase the healing rate of injured digital flexor tendons is a new application of gene therapy. Adenoviral, adeno-associated viral (AAV), and liposome–plasmid vectors have been used to deliver genes to tendons, but the tendon reactions to these vectors—particularly in contrast to the healing responses in the injured tendons—were unknown. This study was designed to compare the tissue reactions of the earlier-mentioned vectors in tendons with the healing responses of injured flexor tendons.
Forty-two flexor digitorum profundus tendons of 6 New Zealand white rabbits were used. Eighteen tendons were divided into 3 groups of 6 each and injected with different vectors: adenoviral vector, AAV2-luciferase vector, or pCMV-β vector with liposome. Another 12 tendons were cut and repaired. At 3, 7, and 14 days, the tendons were harvested and stained with hematoxylin and eosin. Normal flexor tendons were harvested as controls.
The tissue reactions of the liposome–plasmid vector in tendons were the most prominent among the 3 vectors tested. The adenoviral vector elicited a moderate degree of tissue reaction. The AAV2 vector caused remarkable reactions in epitenon but almost no reactions in endotenon. Early-stage tissue reactions were more robust in the injured tendons. Compared with early-stage inflammatory and healing responses, the reactions elicited by these vectors were less severe.
The 3 gene delivery systems tested elicit less severe tissue reactions in flexor tendons compared with early-stage inflammatory changes in injured tendons. Adenoviral and AAV vectors elicit less severe tissue reactions than liposome–plasmid vectors. The AAV2 vector appears to cause almost no reaction in endotenon. In terms of tissue reactions, the adenoviral and AAV2 vectors, in particular AAV2, are suitable gene delivery systems for future gene transfer to the tendon
in vivo. |
| doi_str_mv | 10.1016/j.jhsa.2006.09.007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68212207</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0363502306010252</els_id><sourcerecordid>68212207</sourcerecordid><originalsourceid>FETCH-LOGICAL-c411t-a8c269e2cc0f8a8c789c76fcc1f66e88230f18309ee40cc7410a2b541a7395fe3</originalsourceid><addsrcrecordid>eNp9kc9u1DAQxiMEokvhBTigCAlOJIzt_JW4rFYtrbQSCBY4Wq4zaR0l9taTVPTGO_AcvBRPgtMsqsSBk8ee33zzyV8UPWeQMmDF2y7trkilHKBIoU4BygfRiuWCJUVeZA-jFYhCJDlwcRQ9IeoAwpTIH0dHrGRZLqpyFf3aGaIJ40-o9Gicpdi18bpB626MV_2bpU7WRE4bNWITf13elW3irdk7cgP-_vHzY69oMKGLenSeYmPjHdpmFpzJjRv2yhtyNv5mxqv4RPn-Nvk8qkuMz1D1xl4GC7QPPN5ZOLfd5MO20x6_O_9X62n0qFU94bPDeRx9OT3Zbc6S7Yf355v1NtEZY2OiKs2LGrnW0FbhUla1LotWa9YWBVYVF9CySkCNmIHWZcZA8Ys8Y6oUdd6iOI5eL7p7764npFEOhjT2vbLoJpJFxRnnUAbw5T9g5yZvgzfJGWQ18BICxBdIe0fksZV7bwblbyUDOScpOzknKeckJdQS7pRfHJSniwGb-5FDdAF4dQAUadW3Xllt6J6rRJ7XLAvcu4XD8GE3Br0kbdBqbIwPYcnGmf_5-AOnHr73</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>210490270</pqid></control><display><type>article</type><title>Tissue Reactions of Adenoviral, Adeno-Associated Viral, and Liposome–Plasmid Vectors in Tendons and Comparison With Early-Stage Healing Responses of Injured Flexor Tendons</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Zhu, Bei ; Cao, Yi ; Xin, Ke-Qin ; Wang, Xiao Tian ; Summerhayes, Ian C. ; Liu, Paul Y. ; Tang, Jin Bo</creator><creatorcontrib>Zhu, Bei ; Cao, Yi ; Xin, Ke-Qin ; Wang, Xiao Tian ; Summerhayes, Ian C. ; Liu, Paul Y. ; Tang, Jin Bo</creatorcontrib><description>Delivery of growth factor genes that may substantially increase the healing rate of injured digital flexor tendons is a new application of gene therapy. Adenoviral, adeno-associated viral (AAV), and liposome–plasmid vectors have been used to deliver genes to tendons, but the tendon reactions to these vectors—particularly in contrast to the healing responses in the injured tendons—were unknown. This study was designed to compare the tissue reactions of the earlier-mentioned vectors in tendons with the healing responses of injured flexor tendons.
Forty-two flexor digitorum profundus tendons of 6 New Zealand white rabbits were used. Eighteen tendons were divided into 3 groups of 6 each and injected with different vectors: adenoviral vector, AAV2-luciferase vector, or pCMV-β vector with liposome. Another 12 tendons were cut and repaired. At 3, 7, and 14 days, the tendons were harvested and stained with hematoxylin and eosin. Normal flexor tendons were harvested as controls.
The tissue reactions of the liposome–plasmid vector in tendons were the most prominent among the 3 vectors tested. The adenoviral vector elicited a moderate degree of tissue reaction. The AAV2 vector caused remarkable reactions in epitenon but almost no reactions in endotenon. Early-stage tissue reactions were more robust in the injured tendons. Compared with early-stage inflammatory and healing responses, the reactions elicited by these vectors were less severe.
The 3 gene delivery systems tested elicit less severe tissue reactions in flexor tendons compared with early-stage inflammatory changes in injured tendons. Adenoviral and AAV vectors elicit less severe tissue reactions than liposome–plasmid vectors. The AAV2 vector appears to cause almost no reaction in endotenon. In terms of tissue reactions, the adenoviral and AAV2 vectors, in particular AAV2, are suitable gene delivery systems for future gene transfer to the tendon
in vivo.</description><identifier>ISSN: 0363-5023</identifier><identifier>EISSN: 1531-6564</identifier><identifier>DOI: 10.1016/j.jhsa.2006.09.007</identifier><identifier>PMID: 17145387</identifier><identifier>CODEN: JHSUDV</identifier><language>eng</language><publisher>New york, NY: Elsevier Inc</publisher><subject>adeno-associated viral vectors ; adenoviral vectors ; Animals ; beta-Galactosidase - genetics ; Biological and medical sciences ; Cell Proliferation ; Cytomegalovirus - genetics ; Flexor tendon ; gene therapy ; Genetic Vectors ; healing response ; Inflammation - pathology ; Liposomes ; Luciferases - genetics ; Lymphocytes - pathology ; Medical sciences ; Neutrophils - pathology ; Orthopedic surgery ; Plasmids - genetics ; Rabbits ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tendon Injuries - pathology ; Tendons - pathology ; tissue reactions ; Wound Healing - genetics</subject><ispartof>The Journal of hand surgery (American ed.), 2006-12, Vol.31 (10), p.1652-1660</ispartof><rights>2006 American Society for Surgery of the Hand</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Churchill Livingstone Inc., Medical Publishers Dec 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-a8c269e2cc0f8a8c789c76fcc1f66e88230f18309ee40cc7410a2b541a7395fe3</citedby><cites>FETCH-LOGICAL-c411t-a8c269e2cc0f8a8c789c76fcc1f66e88230f18309ee40cc7410a2b541a7395fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0363502306010252$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18355914$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17145387$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Bei</creatorcontrib><creatorcontrib>Cao, Yi</creatorcontrib><creatorcontrib>Xin, Ke-Qin</creatorcontrib><creatorcontrib>Wang, Xiao Tian</creatorcontrib><creatorcontrib>Summerhayes, Ian C.</creatorcontrib><creatorcontrib>Liu, Paul Y.</creatorcontrib><creatorcontrib>Tang, Jin Bo</creatorcontrib><title>Tissue Reactions of Adenoviral, Adeno-Associated Viral, and Liposome–Plasmid Vectors in Tendons and Comparison With Early-Stage Healing Responses of Injured Flexor Tendons</title><title>The Journal of hand surgery (American ed.)</title><addtitle>J Hand Surg Am</addtitle><description>Delivery of growth factor genes that may substantially increase the healing rate of injured digital flexor tendons is a new application of gene therapy. Adenoviral, adeno-associated viral (AAV), and liposome–plasmid vectors have been used to deliver genes to tendons, but the tendon reactions to these vectors—particularly in contrast to the healing responses in the injured tendons—were unknown. This study was designed to compare the tissue reactions of the earlier-mentioned vectors in tendons with the healing responses of injured flexor tendons.
Forty-two flexor digitorum profundus tendons of 6 New Zealand white rabbits were used. Eighteen tendons were divided into 3 groups of 6 each and injected with different vectors: adenoviral vector, AAV2-luciferase vector, or pCMV-β vector with liposome. Another 12 tendons were cut and repaired. At 3, 7, and 14 days, the tendons were harvested and stained with hematoxylin and eosin. Normal flexor tendons were harvested as controls.
The tissue reactions of the liposome–plasmid vector in tendons were the most prominent among the 3 vectors tested. The adenoviral vector elicited a moderate degree of tissue reaction. The AAV2 vector caused remarkable reactions in epitenon but almost no reactions in endotenon. Early-stage tissue reactions were more robust in the injured tendons. Compared with early-stage inflammatory and healing responses, the reactions elicited by these vectors were less severe.
The 3 gene delivery systems tested elicit less severe tissue reactions in flexor tendons compared with early-stage inflammatory changes in injured tendons. Adenoviral and AAV vectors elicit less severe tissue reactions than liposome–plasmid vectors. The AAV2 vector appears to cause almost no reaction in endotenon. In terms of tissue reactions, the adenoviral and AAV2 vectors, in particular AAV2, are suitable gene delivery systems for future gene transfer to the tendon
in vivo.</description><subject>adeno-associated viral vectors</subject><subject>adenoviral vectors</subject><subject>Animals</subject><subject>beta-Galactosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation</subject><subject>Cytomegalovirus - genetics</subject><subject>Flexor tendon</subject><subject>gene therapy</subject><subject>Genetic Vectors</subject><subject>healing response</subject><subject>Inflammation - pathology</subject><subject>Liposomes</subject><subject>Luciferases - genetics</subject><subject>Lymphocytes - pathology</subject><subject>Medical sciences</subject><subject>Neutrophils - pathology</subject><subject>Orthopedic surgery</subject><subject>Plasmids - genetics</subject><subject>Rabbits</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Tendon Injuries - pathology</subject><subject>Tendons - pathology</subject><subject>tissue reactions</subject><subject>Wound Healing - genetics</subject><issn>0363-5023</issn><issn>1531-6564</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQxiMEokvhBTigCAlOJIzt_JW4rFYtrbQSCBY4Wq4zaR0l9taTVPTGO_AcvBRPgtMsqsSBk8ee33zzyV8UPWeQMmDF2y7trkilHKBIoU4BygfRiuWCJUVeZA-jFYhCJDlwcRQ9IeoAwpTIH0dHrGRZLqpyFf3aGaIJ40-o9Gicpdi18bpB626MV_2bpU7WRE4bNWITf13elW3irdk7cgP-_vHzY69oMKGLenSeYmPjHdpmFpzJjRv2yhtyNv5mxqv4RPn-Nvk8qkuMz1D1xl4GC7QPPN5ZOLfd5MO20x6_O_9X62n0qFU94bPDeRx9OT3Zbc6S7Yf355v1NtEZY2OiKs2LGrnW0FbhUla1LotWa9YWBVYVF9CySkCNmIHWZcZA8Ys8Y6oUdd6iOI5eL7p7764npFEOhjT2vbLoJpJFxRnnUAbw5T9g5yZvgzfJGWQ18BICxBdIe0fksZV7bwblbyUDOScpOzknKeckJdQS7pRfHJSniwGb-5FDdAF4dQAUadW3Xllt6J6rRJ7XLAvcu4XD8GE3Br0kbdBqbIwPYcnGmf_5-AOnHr73</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Zhu, Bei</creator><creator>Cao, Yi</creator><creator>Xin, Ke-Qin</creator><creator>Wang, Xiao Tian</creator><creator>Summerhayes, Ian C.</creator><creator>Liu, Paul Y.</creator><creator>Tang, Jin Bo</creator><general>Elsevier Inc</general><general>Elsevier</general><general>Elsevier Science Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Tissue Reactions of Adenoviral, Adeno-Associated Viral, and Liposome–Plasmid Vectors in Tendons and Comparison With Early-Stage Healing Responses of Injured Flexor Tendons</title><author>Zhu, Bei ; Cao, Yi ; Xin, Ke-Qin ; Wang, Xiao Tian ; Summerhayes, Ian C. ; Liu, Paul Y. ; Tang, Jin Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-a8c269e2cc0f8a8c789c76fcc1f66e88230f18309ee40cc7410a2b541a7395fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>adeno-associated viral vectors</topic><topic>adenoviral vectors</topic><topic>Animals</topic><topic>beta-Galactosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation</topic><topic>Cytomegalovirus - genetics</topic><topic>Flexor tendon</topic><topic>gene therapy</topic><topic>Genetic Vectors</topic><topic>healing response</topic><topic>Inflammation - pathology</topic><topic>Liposomes</topic><topic>Luciferases - genetics</topic><topic>Lymphocytes - pathology</topic><topic>Medical sciences</topic><topic>Neutrophils - pathology</topic><topic>Orthopedic surgery</topic><topic>Plasmids - genetics</topic><topic>Rabbits</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Tendon Injuries - pathology</topic><topic>Tendons - pathology</topic><topic>tissue reactions</topic><topic>Wound Healing - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Bei</creatorcontrib><creatorcontrib>Cao, Yi</creatorcontrib><creatorcontrib>Xin, Ke-Qin</creatorcontrib><creatorcontrib>Wang, Xiao Tian</creatorcontrib><creatorcontrib>Summerhayes, Ian C.</creatorcontrib><creatorcontrib>Liu, Paul Y.</creatorcontrib><creatorcontrib>Tang, Jin Bo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of hand surgery (American ed.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Bei</au><au>Cao, Yi</au><au>Xin, Ke-Qin</au><au>Wang, Xiao Tian</au><au>Summerhayes, Ian C.</au><au>Liu, Paul Y.</au><au>Tang, Jin Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue Reactions of Adenoviral, Adeno-Associated Viral, and Liposome–Plasmid Vectors in Tendons and Comparison With Early-Stage Healing Responses of Injured Flexor Tendons</atitle><jtitle>The Journal of hand surgery (American ed.)</jtitle><addtitle>J Hand Surg Am</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>31</volume><issue>10</issue><spage>1652</spage><epage>1660</epage><pages>1652-1660</pages><issn>0363-5023</issn><eissn>1531-6564</eissn><coden>JHSUDV</coden><abstract>Delivery of growth factor genes that may substantially increase the healing rate of injured digital flexor tendons is a new application of gene therapy. Adenoviral, adeno-associated viral (AAV), and liposome–plasmid vectors have been used to deliver genes to tendons, but the tendon reactions to these vectors—particularly in contrast to the healing responses in the injured tendons—were unknown. This study was designed to compare the tissue reactions of the earlier-mentioned vectors in tendons with the healing responses of injured flexor tendons.
Forty-two flexor digitorum profundus tendons of 6 New Zealand white rabbits were used. Eighteen tendons were divided into 3 groups of 6 each and injected with different vectors: adenoviral vector, AAV2-luciferase vector, or pCMV-β vector with liposome. Another 12 tendons were cut and repaired. At 3, 7, and 14 days, the tendons were harvested and stained with hematoxylin and eosin. Normal flexor tendons were harvested as controls.
The tissue reactions of the liposome–plasmid vector in tendons were the most prominent among the 3 vectors tested. The adenoviral vector elicited a moderate degree of tissue reaction. The AAV2 vector caused remarkable reactions in epitenon but almost no reactions in endotenon. Early-stage tissue reactions were more robust in the injured tendons. Compared with early-stage inflammatory and healing responses, the reactions elicited by these vectors were less severe.
The 3 gene delivery systems tested elicit less severe tissue reactions in flexor tendons compared with early-stage inflammatory changes in injured tendons. Adenoviral and AAV vectors elicit less severe tissue reactions than liposome–plasmid vectors. The AAV2 vector appears to cause almost no reaction in endotenon. In terms of tissue reactions, the adenoviral and AAV2 vectors, in particular AAV2, are suitable gene delivery systems for future gene transfer to the tendon
in vivo.</abstract><cop>New york, NY</cop><pub>Elsevier Inc</pub><pmid>17145387</pmid><doi>10.1016/j.jhsa.2006.09.007</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-5023 |
| ispartof | The Journal of hand surgery (American ed.), 2006-12, Vol.31 (10), p.1652-1660 |
| issn | 0363-5023 1531-6564 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68212207 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | adeno-associated viral vectors adenoviral vectors Animals beta-Galactosidase - genetics Biological and medical sciences Cell Proliferation Cytomegalovirus - genetics Flexor tendon gene therapy Genetic Vectors healing response Inflammation - pathology Liposomes Luciferases - genetics Lymphocytes - pathology Medical sciences Neutrophils - pathology Orthopedic surgery Plasmids - genetics Rabbits Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tendon Injuries - pathology Tendons - pathology tissue reactions Wound Healing - genetics |
| title | Tissue Reactions of Adenoviral, Adeno-Associated Viral, and Liposome–Plasmid Vectors in Tendons and Comparison With Early-Stage Healing Responses of Injured Flexor Tendons |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T18%3A04%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tissue%20Reactions%20of%20Adenoviral,%20Adeno-Associated%20Viral,%20and%20Liposome%E2%80%93Plasmid%20Vectors%20in%20Tendons%20and%20Comparison%20With%20Early-Stage%20Healing%20Responses%20of%20Injured%20Flexor%20Tendons&rft.jtitle=The%20Journal%20of%20hand%20surgery%20(American%20ed.)&rft.au=Zhu,%20Bei&rft.date=2006-12-01&rft.volume=31&rft.issue=10&rft.spage=1652&rft.epage=1660&rft.pages=1652-1660&rft.issn=0363-5023&rft.eissn=1531-6564&rft.coden=JHSUDV&rft_id=info:doi/10.1016/j.jhsa.2006.09.007&rft_dat=%3Cproquest_cross%3E68212207%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=210490270&rft_id=info:pmid/17145387&rft_els_id=S0363502306010252&rfr_iscdi=true |