Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation
Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year...
Gespeichert in:
| Veröffentlicht in: | Virchows Archiv : an international journal of pathology 2007-09, Vol.451 (3), p.691-699 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 699 |
|---|---|
| container_issue | 3 |
| container_start_page | 691 |
| container_title | Virchows Archiv : an international journal of pathology |
| container_volume | 451 |
| creator | KUDO, Naoko OGOSE, Akira HOTTA, Tetsuo KAWASHIMA, Hiroyuki WENGUANG GU UMEZU, Hajime TOYAMA, Tsuyoshi ENDO, Naoto |
| description | Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year-old man. We established the cell line (NDCS-1) from a pleural effusion of the metastatic lung tumor. The cell line was characterized by a the G-banded karyotype, polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, spectral karyotyping, and reverse transcriptase PCR (RT-PCR). The tumor exhibited complex karyotypes and a high frequency of chromosomal amplication with p53 mutation. This tumor revealed an osteoblastic and chondroblastic character in vitro and in severe combined immunodeficiency mice. The expression and phosphorylation of platelet-derived growth factor receptor-beta, which seemed to play a major role in the malignant phenotype of chondrosarcoma, was confirmed by RT-PCR and Western blotting. To our knowledge, this is the first report of the establishment of a human dedifferentiated chondrosarcoma. |
| doi_str_mv | 10.1007/s00428-007-0426-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68211306</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2220030831</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-904b072e5ef88e93cb452ce4399a1892f89cbea1157c26ca55e72e4542cf62623</originalsourceid><addsrcrecordid>eNpdkV9r2zAUxcXoWNJsH2AvQxS6N2_6Y8nWYwnpOgjsZXsWsnyFFWwpleyWffsqJNDSp3vg_s7lSAehr5T8oIQ0PzMhNWurIqsiZMU_oDWtOasYJ80VWhNVi0py2qzQdc4HQhhtqfyEVrSRgjeSrdGwy7PpRp-HCcKMo8MhPsGIh2UyAffQe-cglZU3M_TYDjH0KWaTbJwMtjCOePQB8LOfBxzzDLEbTZ69xW-dPobP6KMzY4Yvl7lB_-53f7cP1f7Pr9_bu31luZBzpUjdkYaBANe2oLjtasEs1FwpQ1vFXKtsB4ZS0VgmrRECCl2LmlknmWR8g76f7x5TfFwgz3ry-ZTTBIhL1rJllHIiC3jzDjzEJYWSTbeMSMWEUgWiZ8iWR-cETh-Tn0z6rynRpw70uQN9kqcONC-eb5fDSzdB_-q4fHoBbi-AydaMLplgfX7lVEknZcNfADAxkBE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>820692599</pqid></control><display><type>article</type><title>Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>KUDO, Naoko ; OGOSE, Akira ; HOTTA, Tetsuo ; KAWASHIMA, Hiroyuki ; WENGUANG GU ; UMEZU, Hajime ; TOYAMA, Tsuyoshi ; ENDO, Naoto</creator><creatorcontrib>KUDO, Naoko ; OGOSE, Akira ; HOTTA, Tetsuo ; KAWASHIMA, Hiroyuki ; WENGUANG GU ; UMEZU, Hajime ; TOYAMA, Tsuyoshi ; ENDO, Naoto</creatorcontrib><description>Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year-old man. We established the cell line (NDCS-1) from a pleural effusion of the metastatic lung tumor. The cell line was characterized by a the G-banded karyotype, polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, spectral karyotyping, and reverse transcriptase PCR (RT-PCR). The tumor exhibited complex karyotypes and a high frequency of chromosomal amplication with p53 mutation. This tumor revealed an osteoblastic and chondroblastic character in vitro and in severe combined immunodeficiency mice. The expression and phosphorylation of platelet-derived growth factor receptor-beta, which seemed to play a major role in the malignant phenotype of chondrosarcoma, was confirmed by RT-PCR and Western blotting. To our knowledge, this is the first report of the establishment of a human dedifferentiated chondrosarcoma.</description><identifier>ISSN: 0945-6317</identifier><identifier>EISSN: 1432-2307</identifier><identifier>DOI: 10.1007/s00428-007-0426-3</identifier><identifier>PMID: 17653762</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Adult ; Animals ; Bacteria ; Biological and medical sciences ; Biomedical materials ; Blotting, Western ; Cell Differentiation ; Cell Division ; Cell Line, Tumor ; Chondrosarcoma ; Chondrosarcoma - genetics ; Chondrosarcoma - pathology ; Chromosome Aberrations ; Conformation ; Diseases of the osteoarticular system ; Femoral Neoplasms - genetics ; Femoral Neoplasms - pathology ; Femur ; Genes, p53 - genetics ; Growth factors ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Karyotypes ; Karyotyping ; Lung Neoplasms - pathology ; Lung Neoplasms - secondary ; Male ; Medical sciences ; Metastases ; Mice ; Mice, SCID ; Molecular conformation ; Mutation ; Neoplasm Transplantation ; Osteoblastogenesis ; Osteoblasts ; Osteoblasts - pathology ; Osteosarcoma ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Phenotypes ; Phosphorylation ; Platelet-derived growth factor ; Platelet-derived growth factor receptors ; Pleural effusion ; Pleural Effusion - cytology ; Polymerase chain reaction ; Polymorphism ; Polymorphism, Single-Stranded Conformational ; Receptor, Platelet-Derived Growth Factor beta - genetics ; Receptor, Platelet-Derived Growth Factor beta - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA-directed DNA polymerase ; Sarcoma ; Severe combined immunodeficiency ; Tumors ; Tumors of striated muscle and skeleton ; Western blotting</subject><ispartof>Virchows Archiv : an international journal of pathology, 2007-09, Vol.451 (3), p.691-699</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2007.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-904b072e5ef88e93cb452ce4399a1892f89cbea1157c26ca55e72e4542cf62623</citedby><cites>FETCH-LOGICAL-c356t-904b072e5ef88e93cb452ce4399a1892f89cbea1157c26ca55e72e4542cf62623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19063667$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17653762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KUDO, Naoko</creatorcontrib><creatorcontrib>OGOSE, Akira</creatorcontrib><creatorcontrib>HOTTA, Tetsuo</creatorcontrib><creatorcontrib>KAWASHIMA, Hiroyuki</creatorcontrib><creatorcontrib>WENGUANG GU</creatorcontrib><creatorcontrib>UMEZU, Hajime</creatorcontrib><creatorcontrib>TOYAMA, Tsuyoshi</creatorcontrib><creatorcontrib>ENDO, Naoto</creatorcontrib><title>Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation</title><title>Virchows Archiv : an international journal of pathology</title><addtitle>Virchows Arch</addtitle><description>Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year-old man. We established the cell line (NDCS-1) from a pleural effusion of the metastatic lung tumor. The cell line was characterized by a the G-banded karyotype, polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, spectral karyotyping, and reverse transcriptase PCR (RT-PCR). The tumor exhibited complex karyotypes and a high frequency of chromosomal amplication with p53 mutation. This tumor revealed an osteoblastic and chondroblastic character in vitro and in severe combined immunodeficiency mice. The expression and phosphorylation of platelet-derived growth factor receptor-beta, which seemed to play a major role in the malignant phenotype of chondrosarcoma, was confirmed by RT-PCR and Western blotting. To our knowledge, this is the first report of the establishment of a human dedifferentiated chondrosarcoma.</description><subject>Adult</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biomedical materials</subject><subject>Blotting, Western</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Cell Line, Tumor</subject><subject>Chondrosarcoma</subject><subject>Chondrosarcoma - genetics</subject><subject>Chondrosarcoma - pathology</subject><subject>Chromosome Aberrations</subject><subject>Conformation</subject><subject>Diseases of the osteoarticular system</subject><subject>Femoral Neoplasms - genetics</subject><subject>Femoral Neoplasms - pathology</subject><subject>Femur</subject><subject>Genes, p53 - genetics</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Karyotypes</subject><subject>Karyotyping</subject><subject>Lung Neoplasms - pathology</subject><subject>Lung Neoplasms - secondary</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metastases</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Molecular conformation</subject><subject>Mutation</subject><subject>Neoplasm Transplantation</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteoblasts - pathology</subject><subject>Osteosarcoma</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Phenotypes</subject><subject>Phosphorylation</subject><subject>Platelet-derived growth factor</subject><subject>Platelet-derived growth factor receptors</subject><subject>Pleural effusion</subject><subject>Pleural Effusion - cytology</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Receptor, Platelet-Derived Growth Factor beta - genetics</subject><subject>Receptor, Platelet-Derived Growth Factor beta - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA-directed DNA polymerase</subject><subject>Sarcoma</subject><subject>Severe combined immunodeficiency</subject><subject>Tumors</subject><subject>Tumors of striated muscle and skeleton</subject><subject>Western blotting</subject><issn>0945-6317</issn><issn>1432-2307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkV9r2zAUxcXoWNJsH2AvQxS6N2_6Y8nWYwnpOgjsZXsWsnyFFWwpleyWffsqJNDSp3vg_s7lSAehr5T8oIQ0PzMhNWurIqsiZMU_oDWtOasYJ80VWhNVi0py2qzQdc4HQhhtqfyEVrSRgjeSrdGwy7PpRp-HCcKMo8MhPsGIh2UyAffQe-cglZU3M_TYDjH0KWaTbJwMtjCOePQB8LOfBxzzDLEbTZ69xW-dPobP6KMzY4Yvl7lB_-53f7cP1f7Pr9_bu31luZBzpUjdkYaBANe2oLjtasEs1FwpQ1vFXKtsB4ZS0VgmrRECCl2LmlknmWR8g76f7x5TfFwgz3ry-ZTTBIhL1rJllHIiC3jzDjzEJYWSTbeMSMWEUgWiZ8iWR-cETh-Tn0z6rynRpw70uQN9kqcONC-eb5fDSzdB_-q4fHoBbi-AydaMLplgfX7lVEknZcNfADAxkBE</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>KUDO, Naoko</creator><creator>OGOSE, Akira</creator><creator>HOTTA, Tetsuo</creator><creator>KAWASHIMA, Hiroyuki</creator><creator>WENGUANG GU</creator><creator>UMEZU, Hajime</creator><creator>TOYAMA, Tsuyoshi</creator><creator>ENDO, Naoto</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20070901</creationdate><title>Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation</title><author>KUDO, Naoko ; OGOSE, Akira ; HOTTA, Tetsuo ; KAWASHIMA, Hiroyuki ; WENGUANG GU ; UMEZU, Hajime ; TOYAMA, Tsuyoshi ; ENDO, Naoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-904b072e5ef88e93cb452ce4399a1892f89cbea1157c26ca55e72e4542cf62623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Biomedical materials</topic><topic>Blotting, Western</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Cell Line, Tumor</topic><topic>Chondrosarcoma</topic><topic>Chondrosarcoma - genetics</topic><topic>Chondrosarcoma - pathology</topic><topic>Chromosome Aberrations</topic><topic>Conformation</topic><topic>Diseases of the osteoarticular system</topic><topic>Femoral Neoplasms - genetics</topic><topic>Femoral Neoplasms - pathology</topic><topic>Femur</topic><topic>Genes, p53 - genetics</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Karyotypes</topic><topic>Karyotyping</topic><topic>Lung Neoplasms - pathology</topic><topic>Lung Neoplasms - secondary</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metastases</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Molecular conformation</topic><topic>Mutation</topic><topic>Neoplasm Transplantation</topic><topic>Osteoblastogenesis</topic><topic>Osteoblasts</topic><topic>Osteoblasts - pathology</topic><topic>Osteosarcoma</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Phenotypes</topic><topic>Phosphorylation</topic><topic>Platelet-derived growth factor</topic><topic>Platelet-derived growth factor receptors</topic><topic>Pleural effusion</topic><topic>Pleural Effusion - cytology</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Receptor, Platelet-Derived Growth Factor beta - genetics</topic><topic>Receptor, Platelet-Derived Growth Factor beta - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA-directed DNA polymerase</topic><topic>Sarcoma</topic><topic>Severe combined immunodeficiency</topic><topic>Tumors</topic><topic>Tumors of striated muscle and skeleton</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KUDO, Naoko</creatorcontrib><creatorcontrib>OGOSE, Akira</creatorcontrib><creatorcontrib>HOTTA, Tetsuo</creatorcontrib><creatorcontrib>KAWASHIMA, Hiroyuki</creatorcontrib><creatorcontrib>WENGUANG GU</creatorcontrib><creatorcontrib>UMEZU, Hajime</creatorcontrib><creatorcontrib>TOYAMA, Tsuyoshi</creatorcontrib><creatorcontrib>ENDO, Naoto</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Virchows Archiv : an international journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KUDO, Naoko</au><au>OGOSE, Akira</au><au>HOTTA, Tetsuo</au><au>KAWASHIMA, Hiroyuki</au><au>WENGUANG GU</au><au>UMEZU, Hajime</au><au>TOYAMA, Tsuyoshi</au><au>ENDO, Naoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation</atitle><jtitle>Virchows Archiv : an international journal of pathology</jtitle><addtitle>Virchows Arch</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>451</volume><issue>3</issue><spage>691</spage><epage>699</epage><pages>691-699</pages><issn>0945-6317</issn><eissn>1432-2307</eissn><abstract>Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year-old man. We established the cell line (NDCS-1) from a pleural effusion of the metastatic lung tumor. The cell line was characterized by a the G-banded karyotype, polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, spectral karyotyping, and reverse transcriptase PCR (RT-PCR). The tumor exhibited complex karyotypes and a high frequency of chromosomal amplication with p53 mutation. This tumor revealed an osteoblastic and chondroblastic character in vitro and in severe combined immunodeficiency mice. The expression and phosphorylation of platelet-derived growth factor receptor-beta, which seemed to play a major role in the malignant phenotype of chondrosarcoma, was confirmed by RT-PCR and Western blotting. To our knowledge, this is the first report of the establishment of a human dedifferentiated chondrosarcoma.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>17653762</pmid><doi>10.1007/s00428-007-0426-3</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0945-6317 |
| ispartof | Virchows Archiv : an international journal of pathology, 2007-09, Vol.451 (3), p.691-699 |
| issn | 0945-6317 1432-2307 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68211306 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Animals Bacteria Biological and medical sciences Biomedical materials Blotting, Western Cell Differentiation Cell Division Cell Line, Tumor Chondrosarcoma Chondrosarcoma - genetics Chondrosarcoma - pathology Chromosome Aberrations Conformation Diseases of the osteoarticular system Femoral Neoplasms - genetics Femoral Neoplasms - pathology Femur Genes, p53 - genetics Growth factors Humans Investigative techniques, diagnostic techniques (general aspects) Karyotypes Karyotyping Lung Neoplasms - pathology Lung Neoplasms - secondary Male Medical sciences Metastases Mice Mice, SCID Molecular conformation Mutation Neoplasm Transplantation Osteoblastogenesis Osteoblasts Osteoblasts - pathology Osteosarcoma Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Phenotypes Phosphorylation Platelet-derived growth factor Platelet-derived growth factor receptors Pleural effusion Pleural Effusion - cytology Polymerase chain reaction Polymorphism Polymorphism, Single-Stranded Conformational Receptor, Platelet-Derived Growth Factor beta - genetics Receptor, Platelet-Derived Growth Factor beta - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA-directed DNA polymerase Sarcoma Severe combined immunodeficiency Tumors Tumors of striated muscle and skeleton Western blotting |
| title | Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T22%3A25%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Establishment%20of%20novel%20human%20dedifferentiated%20chondrosarcoma%20cell%20line%20with%20osteoblastic%20differentiation&rft.jtitle=Virchows%20Archiv%20:%20an%20international%20journal%20of%20pathology&rft.au=KUDO,%20Naoko&rft.date=2007-09-01&rft.volume=451&rft.issue=3&rft.spage=691&rft.epage=699&rft.pages=691-699&rft.issn=0945-6317&rft.eissn=1432-2307&rft_id=info:doi/10.1007/s00428-007-0426-3&rft_dat=%3Cproquest_cross%3E2220030831%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=820692599&rft_id=info:pmid/17653762&rfr_iscdi=true |