Heterologous expression, purification, and properties of a potato protein inhibitor of serine proteinases

The gene PKPI-B10 [AF536175] encoding in potato (Solanum tuberosum L., cv. Istrinskii) a Kunitz-type protein inhibitor of proteinases (PKPI) has been cloned into the pET23a vector and then expressed in Escherichia coli. The recombinant protein PKPI-B10 obtained as inclusion bodies was denatured, sep...

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Veröffentlicht in:	Biochemistry (Moscow) 2006-11, Vol.71 (11), p.1176-1182
Hauptverfasser:	Speranskaya, A S, Krinitsina, A A, Revina, T A, Gerasimova, N G, Keruchen'ko, Ya S, Shevelev, A B, Valueva, T A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Cloning, Molecular E coli Enzymes Escherichia coli Escherichia coli - metabolism Ion exchange Liquid chromatography Molecular Sequence Data Potatoes Protein folding Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Sequence Homology, Amino Acid Serine Proteinase Inhibitors - genetics Serine Proteinase Inhibitors - isolation & purification Serine Proteinase Inhibitors - metabolism Solanum tuberosum Solanum tuberosum - genetics Transformation, Genetic
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