Analysis of intracellular short organic acid-coenzyme A esters from actinomycetes using liquid chromatography-electrospray ionization-mass spectrometry

A method employing silicone oil density centrifugation, solid-phase extraction (SPE) cleanup, and LC-ESI-MS/MS analysis was developed for the rapid, selective, sensitive, and quantitative detection of an intracellular pool of short organic acid-CoA esters in actinomycetes. The detection limit was de...

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Veröffentlicht in:	Journal of mass spectrometry. 2007-09, Vol.42 (9), p.1136-1147
Hauptverfasser:	Park, Je Won, Jung, Won Seok, Park, Sung Ryeol, Park, Byoung Chul, Yoon, Yeo Joon
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinobacteria - metabolism actinomycetes Acyl Coenzyme A - analysis Bacterial Proteins Bacteriology Biological and medical sciences Chromatography, Liquid CoA-esters Coenzyme A - analysis Esters - analysis Fundamental and applied biological sciences. Psychology LC-ESI-MS/MS metabolomics Microbiology Morphology, structure, chemical composition polyketide Polyketide Synthases - analysis Spectrometry, Mass, Electrospray Ionization Streptomyces - metabolism
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