Long-Term Field Release of Bioluminescent Sinorhizobium meliloti Strains to Assess the Influence of a recA Mutation on the Strains' Survival

A field release experiment was carried out to study the fate of the isogenic, firefly luciferase (luc) gene-tagged Sinorhizobium meliloti strains L1 (RecA-) and L33 (RecA⁺) in the environment. Both strains were released at concentrations of approximately 10⁶ cfu g-¹ soil in replicate and randomized...

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Veröffentlicht in:Microbial ecology 2006-10, Vol.52 (3), p.583-595
Hauptverfasser: Selbitschka, W, Keller, M, Miethling-Graff, R, Dresing, U, Schwieger, F, Krahn, I, Homann, I, Dammann-Kalinowski, T, Pühler, A, Tebbe, C. C
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container_issue 3
container_start_page 583
container_title Microbial ecology
container_volume 52
creator Selbitschka, W
Keller, M
Miethling-Graff, R
Dresing, U
Schwieger, F
Krahn, I
Homann, I
Dammann-Kalinowski, T
Pühler, A
Tebbe, C. C
description A field release experiment was carried out to study the fate of the isogenic, firefly luciferase (luc) gene-tagged Sinorhizobium meliloti strains L1 (RecA-) and L33 (RecA⁺) in the environment. Both strains were released at concentrations of approximately 10⁶ cfu g-¹ soil in replicate and randomized field plots, which had been sown with alfalfa (Medicago sativa). The survival of both strains during the following 7 years could be subdivided into three phases: a sharp decline for more than two orders of magnitude within the first 4 months (phase I), followed by fluctuations around an average number of 10⁴ cfu g-¹ soil for nearly 4 years (phase II), and a further decline to approximately 60 cfu g-¹ (phase III). At most sampling dates, no significant differences in the survival of both strains were detected, indicating that the recA gene function was dispensable under these environmental conditions. During the field inoculation, both strains were dispersed accidentally by wind in small numbers to noninoculated field plots. Strain L33 established at a concentration of more than 10³ cfu g-¹ soil with subsequent seasonal fluctuations. Although strain L1 must have been disseminated to a similar extent, it could never be recovered from noninoculated field plots, indicating that the recA mutation interfered with the strain's capability to establish there. At the beginning of the field experiment, an indigenous alfalfa-nodulating population was below the limit of detection. In the following years, however, an indigenous population arose, which finally outcompeted both strains for saprophytic growth and alfalfa nodulation. RecA- strain L1 was outcompeted for alfalfa nodulation slightly faster than its RecA⁺ counterpart L33. The diversity of the indigenous population was characterized by employing the Enterobacterial Repetitive Intergenic Consensus polymerase chain reaction fingerprint method. Typing of 2731 root nodule isolates revealed a total of 38 fingerprint groups. More than 80% of the isolates could be grouped into six dominant fingerprint groups, indicating that a few dominant bacterial strain types had outcompeted the released strains.
doi_str_mv 10.1007/s00248-006-9056-6
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C</creator><creatorcontrib>Selbitschka, W ; Keller, M ; Miethling-Graff, R ; Dresing, U ; Schwieger, F ; Krahn, I ; Homann, I ; Dammann-Kalinowski, T ; Pühler, A ; Tebbe, C. C</creatorcontrib><description>A field release experiment was carried out to study the fate of the isogenic, firefly luciferase (luc) gene-tagged Sinorhizobium meliloti strains L1 (RecA-) and L33 (RecA⁺) in the environment. Both strains were released at concentrations of approximately 10⁶ cfu g-¹ soil in replicate and randomized field plots, which had been sown with alfalfa (Medicago sativa). The survival of both strains during the following 7 years could be subdivided into three phases: a sharp decline for more than two orders of magnitude within the first 4 months (phase I), followed by fluctuations around an average number of 10⁴ cfu g-¹ soil for nearly 4 years (phase II), and a further decline to approximately 60 cfu g-¹ (phase III). At most sampling dates, no significant differences in the survival of both strains were detected, indicating that the recA gene function was dispensable under these environmental conditions. During the field inoculation, both strains were dispersed accidentally by wind in small numbers to noninoculated field plots. Strain L33 established at a concentration of more than 10³ cfu g-¹ soil with subsequent seasonal fluctuations. Although strain L1 must have been disseminated to a similar extent, it could never be recovered from noninoculated field plots, indicating that the recA mutation interfered with the strain's capability to establish there. At the beginning of the field experiment, an indigenous alfalfa-nodulating population was below the limit of detection. In the following years, however, an indigenous population arose, which finally outcompeted both strains for saprophytic growth and alfalfa nodulation. RecA- strain L1 was outcompeted for alfalfa nodulation slightly faster than its RecA⁺ counterpart L33. The diversity of the indigenous population was characterized by employing the Enterobacterial Repetitive Intergenic Consensus polymerase chain reaction fingerprint method. Typing of 2731 root nodule isolates revealed a total of 38 fingerprint groups. 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Psychology ; Gene Transfer, Horizontal ; Indigenous populations ; Inoculation ; Luminescence ; Medicago sativa ; Medicago sativa - microbiology ; Microbiology ; Miscellaneous ; Mutation ; Nitrogen - metabolism ; Nodules ; Organisms, Genetically Modified ; Phenotype ; Polymerase Chain Reaction ; Random Allocation ; Rec A Recombinases - genetics ; Root nodules ; Seasons ; Sinorhizobium meliloti ; Sinorhizobium meliloti - genetics ; Sinorhizobium meliloti - growth &amp; development ; Soil inoculation ; Soil Microbiology ; Soil samples</subject><ispartof>Microbial ecology, 2006-10, Vol.52 (3), p.583-595</ispartof><rights>Copyright 2006 Springer Science+Business Media, Inc.</rights><rights>2007 INIST-CNRS</rights><rights>Springer Science+Business Media, LLC 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-d53c2492c4c2650e8df8b69728b88dbcbfad583c6bd3d38dd8adac951dfbe0a23</citedby><cites>FETCH-LOGICAL-c433t-d53c2492c4c2650e8df8b69728b88dbcbfad583c6bd3d38dd8adac951dfbe0a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25153411$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25153411$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57996,58229</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=18353224$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16924432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Selbitschka, W</creatorcontrib><creatorcontrib>Keller, M</creatorcontrib><creatorcontrib>Miethling-Graff, R</creatorcontrib><creatorcontrib>Dresing, U</creatorcontrib><creatorcontrib>Schwieger, F</creatorcontrib><creatorcontrib>Krahn, I</creatorcontrib><creatorcontrib>Homann, I</creatorcontrib><creatorcontrib>Dammann-Kalinowski, T</creatorcontrib><creatorcontrib>Pühler, A</creatorcontrib><creatorcontrib>Tebbe, C. C</creatorcontrib><title>Long-Term Field Release of Bioluminescent Sinorhizobium meliloti Strains to Assess the Influence of a recA Mutation on the Strains' Survival</title><title>Microbial ecology</title><addtitle>Microb Ecol</addtitle><description>A field release experiment was carried out to study the fate of the isogenic, firefly luciferase (luc) gene-tagged Sinorhizobium meliloti strains L1 (RecA-) and L33 (RecA⁺) in the environment. Both strains were released at concentrations of approximately 10⁶ cfu g-¹ soil in replicate and randomized field plots, which had been sown with alfalfa (Medicago sativa). The survival of both strains during the following 7 years could be subdivided into three phases: a sharp decline for more than two orders of magnitude within the first 4 months (phase I), followed by fluctuations around an average number of 10⁴ cfu g-¹ soil for nearly 4 years (phase II), and a further decline to approximately 60 cfu g-¹ (phase III). 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RecA- strain L1 was outcompeted for alfalfa nodulation slightly faster than its RecA⁺ counterpart L33. The diversity of the indigenous population was characterized by employing the Enterobacterial Repetitive Intergenic Consensus polymerase chain reaction fingerprint method. Typing of 2731 root nodule isolates revealed a total of 38 fingerprint groups. 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The survival of both strains during the following 7 years could be subdivided into three phases: a sharp decline for more than two orders of magnitude within the first 4 months (phase I), followed by fluctuations around an average number of 10⁴ cfu g-¹ soil for nearly 4 years (phase II), and a further decline to approximately 60 cfu g-¹ (phase III). At most sampling dates, no significant differences in the survival of both strains were detected, indicating that the recA gene function was dispensable under these environmental conditions. During the field inoculation, both strains were dispersed accidentally by wind in small numbers to noninoculated field plots. Strain L33 established at a concentration of more than 10³ cfu g-¹ soil with subsequent seasonal fluctuations. Although strain L1 must have been disseminated to a similar extent, it could never be recovered from noninoculated field plots, indicating that the recA mutation interfered with the strain's capability to establish there. At the beginning of the field experiment, an indigenous alfalfa-nodulating population was below the limit of detection. In the following years, however, an indigenous population arose, which finally outcompeted both strains for saprophytic growth and alfalfa nodulation. RecA- strain L1 was outcompeted for alfalfa nodulation slightly faster than its RecA⁺ counterpart L33. The diversity of the indigenous population was characterized by employing the Enterobacterial Repetitive Intergenic Consensus polymerase chain reaction fingerprint method. Typing of 2731 root nodule isolates revealed a total of 38 fingerprint groups. More than 80% of the isolates could be grouped into six dominant fingerprint groups, indicating that a few dominant bacterial strain types had outcompeted the released strains.</abstract><cop>New York, NY</cop><pub>New York : Springer-Verlag</pub><pmid>16924432</pmid><doi>10.1007/s00248-006-9056-6</doi><tpages>13</tpages></addata></record>
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identifier ISSN: 0095-3628
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1432-184X
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source MEDLINE; Jstor Complete Legacy; Springer Nature - Complete Springer Journals
subjects Agricultural soils
Air Microbiology
Alfalfa
Bacteria
Bacteriology
Biological and medical sciences
Biomass
Carbon - metabolism
Colony Count, Microbial
DNA Fingerprinting
Ecosystem
Environmental conditions
Fluctuations
Fundamental and applied biological sciences. Psychology
Gene Transfer, Horizontal
Indigenous populations
Inoculation
Luminescence
Medicago sativa
Medicago sativa - microbiology
Microbiology
Miscellaneous
Mutation
Nitrogen - metabolism
Nodules
Organisms, Genetically Modified
Phenotype
Polymerase Chain Reaction
Random Allocation
Rec A Recombinases - genetics
Root nodules
Seasons
Sinorhizobium meliloti
Sinorhizobium meliloti - genetics
Sinorhizobium meliloti - growth & development
Soil inoculation
Soil Microbiology
Soil samples
title Long-Term Field Release of Bioluminescent Sinorhizobium meliloti Strains to Assess the Influence of a recA Mutation on the Strains' Survival
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