Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening
White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to pre...
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| Veröffentlicht in: | Diseases of aquatic organisms 2006-10, Vol.72 (2), p.93-99 |
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| container_title | Diseases of aquatic organisms |
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| creator | LI YUAN XIAOHUA ZHANG NAN XIAO LINGFEN DAI WEIZHOU CHEN CHENSONG JIA RUOHONG ZHAO HEMMINGSEN, Scan M HEPING DAI |
| description | White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease. |
| doi_str_mv | 10.3354/dao072093 |
| format | Article |
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Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.</description><identifier>ISSN: 0177-5103</identifier><identifier>EISSN: 1616-1580</identifier><identifier>DOI: 10.3354/dao072093</identifier><identifier>PMID: 17140131</identifier><identifier>CODEN: DAOREO</identifier><language>eng</language><publisher>Oldendorf: Inter-Research</publisher><subject>Amino Acid Sequence ; Animal aquaculture ; Animal productions ; Animals ; Antibodies, Viral - chemistry ; Antibodies, Viral - genetics ; Antibodies, Viral - immunology ; Antibodies, Viral - isolation & purification ; Base Sequence ; Biological and medical sciences ; Cells, Cultured ; Crustacea ; Decapoda ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Hemolymph - immunology ; Immune Sera - immunology ; Invertebrate aquaculture ; Male ; Marine ; Molecular Sequence Data ; Penaeidae - cytology ; Penaeidae - virology ; Peptide Library ; Sequence Alignment ; Swine ; White spot syndrome virus ; White spot syndrome virus 1 - genetics ; White spot syndrome virus 1 - immunology ; White spot syndrome virus 1 - isolation & purification ; White spot syndrome virus 1 - ultrastructure</subject><ispartof>Diseases of aquatic organisms, 2006-10, Vol.72 (2), p.93-99</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-17bbebf587bab94ea2856ba3d77d9ea842629d768fcea8a9b80eb3de84e5ccd53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3759,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18267903$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17140131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LI YUAN</creatorcontrib><creatorcontrib>XIAOHUA ZHANG</creatorcontrib><creatorcontrib>NAN XIAO</creatorcontrib><creatorcontrib>LINGFEN DAI</creatorcontrib><creatorcontrib>WEIZHOU CHEN</creatorcontrib><creatorcontrib>CHENSONG JIA</creatorcontrib><creatorcontrib>RUOHONG ZHAO</creatorcontrib><creatorcontrib>HEMMINGSEN, Scan M</creatorcontrib><creatorcontrib>HEPING DAI</creatorcontrib><title>Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening</title><title>Diseases of aquatic organisms</title><addtitle>Dis Aquat Organ</addtitle><description>White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.</description><subject>Amino Acid Sequence</subject><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Antibodies, Viral - chemistry</subject><subject>Antibodies, Viral - genetics</subject><subject>Antibodies, Viral - immunology</subject><subject>Antibodies, Viral - isolation & purification</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Crustacea</subject><subject>Decapoda</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemolymph - immunology</subject><subject>Immune Sera - immunology</subject><subject>Invertebrate aquaculture</subject><subject>Male</subject><subject>Marine</subject><subject>Molecular Sequence Data</subject><subject>Penaeidae - cytology</subject><subject>Penaeidae - virology</subject><subject>Peptide Library</subject><subject>Sequence Alignment</subject><subject>Swine</subject><subject>White spot syndrome virus</subject><subject>White spot syndrome virus 1 - genetics</subject><subject>White spot syndrome virus 1 - immunology</subject><subject>White spot syndrome virus 1 - isolation & purification</subject><subject>White spot syndrome virus 1 - ultrastructure</subject><issn>0177-5103</issn><issn>1616-1580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0DtrHDEUBWBhEuyN7cJ_IKhJIMXE0mj0Ko2JE4MhhR8phyvpzlphVlpLs4bxr88EL3GZ6nLhO6c4hJxx9lUI2Z0HyEy3zIoDsuKKq4ZLw96RFeNaN5IzcUQ-1PqbMd5ayQ_JEde8Y1zwFRmuA6YpDtHDFHOieaBAf93ePtCEu6nAGF9iWtPqr54pLNDlMFM30-0jrJGGWLcjzHRC_5jymNfzggKNiT7HqeQlVhDTUnBC3g8wVjzd32Nyf_Xt7vJHc_Pz-_XlxU3jhbZTw7Vz6AZptANnO4TWSOVABK2DRTBdq1obtDKDXz6wzjB0IqDpUHofpDgmn197tyU_7bBO_SZWj-MICfOu9sq0THVS_RdyK7Qwpl3gl1foS6614NBvS9xAmXvO-r_r9__WX-zHfenObTC8yf3cC_i0B1A9jEOB5GN9c6ZV2jIh_gBOO45X</recordid><startdate>20061017</startdate><enddate>20061017</enddate><creator>LI YUAN</creator><creator>XIAOHUA ZHANG</creator><creator>NAN XIAO</creator><creator>LINGFEN DAI</creator><creator>WEIZHOU CHEN</creator><creator>CHENSONG JIA</creator><creator>RUOHONG ZHAO</creator><creator>HEMMINGSEN, Scan M</creator><creator>HEPING DAI</creator><general>Inter-Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20061017</creationdate><title>Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening</title><author>LI YUAN ; XIAOHUA ZHANG ; NAN XIAO ; LINGFEN DAI ; WEIZHOU CHEN ; CHENSONG JIA ; RUOHONG ZHAO ; HEMMINGSEN, Scan M ; HEPING DAI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-17bbebf587bab94ea2856ba3d77d9ea842629d768fcea8a9b80eb3de84e5ccd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Antibodies, Viral - chemistry</topic><topic>Antibodies, Viral - genetics</topic><topic>Antibodies, Viral - immunology</topic><topic>Antibodies, Viral - isolation & purification</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Crustacea</topic><topic>Decapoda</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemolymph - immunology</topic><topic>Immune Sera - immunology</topic><topic>Invertebrate aquaculture</topic><topic>Male</topic><topic>Marine</topic><topic>Molecular Sequence Data</topic><topic>Penaeidae - cytology</topic><topic>Penaeidae - virology</topic><topic>Peptide Library</topic><topic>Sequence Alignment</topic><topic>Swine</topic><topic>White spot syndrome virus</topic><topic>White spot syndrome virus 1 - genetics</topic><topic>White spot syndrome virus 1 - immunology</topic><topic>White spot syndrome virus 1 - isolation & purification</topic><topic>White spot syndrome virus 1 - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LI YUAN</creatorcontrib><creatorcontrib>XIAOHUA ZHANG</creatorcontrib><creatorcontrib>NAN XIAO</creatorcontrib><creatorcontrib>LINGFEN DAI</creatorcontrib><creatorcontrib>WEIZHOU CHEN</creatorcontrib><creatorcontrib>CHENSONG JIA</creatorcontrib><creatorcontrib>RUOHONG ZHAO</creatorcontrib><creatorcontrib>HEMMINGSEN, Scan M</creatorcontrib><creatorcontrib>HEPING DAI</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Diseases of aquatic organisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LI YUAN</au><au>XIAOHUA ZHANG</au><au>NAN XIAO</au><au>LINGFEN DAI</au><au>WEIZHOU CHEN</au><au>CHENSONG JIA</au><au>RUOHONG ZHAO</au><au>HEMMINGSEN, Scan M</au><au>HEPING DAI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening</atitle><jtitle>Diseases of aquatic organisms</jtitle><addtitle>Dis Aquat Organ</addtitle><date>2006-10-17</date><risdate>2006</risdate><volume>72</volume><issue>2</issue><spage>93</spage><epage>99</epage><pages>93-99</pages><issn>0177-5103</issn><eissn>1616-1580</eissn><coden>DAOREO</coden><abstract>White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.</abstract><cop>Oldendorf</cop><pub>Inter-Research</pub><pmid>17140131</pmid><doi>10.3354/dao072093</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Diseases of aquatic organisms, 2006-10, Vol.72 (2), p.93-99 |
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| source | MEDLINE; Inter-Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animal aquaculture Animal productions Animals Antibodies, Viral - chemistry Antibodies, Viral - genetics Antibodies, Viral - immunology Antibodies, Viral - isolation & purification Base Sequence Biological and medical sciences Cells, Cultured Crustacea Decapoda Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Hemolymph - immunology Immune Sera - immunology Invertebrate aquaculture Male Marine Molecular Sequence Data Penaeidae - cytology Penaeidae - virology Peptide Library Sequence Alignment Swine White spot syndrome virus White spot syndrome virus 1 - genetics White spot syndrome virus 1 - immunology White spot syndrome virus 1 - isolation & purification White spot syndrome virus 1 - ultrastructure |
| title | Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening |
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