Pigments from Uroleucon nigrotuberculatum Induce Apoptosis in HL60 Human Leukemia Cells, Implicating Intracellular Oxidative Stress and Activation of Caspases
Two pigmented compounds from Uroleucon nigrotuberculatum, rhododactynaphin-jc-1 (H427) and rhododactynaphin-jc-2 (H373), significantly diminished the cell viability of HL60 cells with IC50 of 10 μM and 30 μM, respectively, in an 18 h-dye uptake assay. Both H427 and H373 augmented the levels of intra...
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| Veröffentlicht in: | Biological & Pharmaceutical Bulletin 2006, Vol.29(12), pp.2383-2387 |
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| creator | Suzuki, Shinya Tomita, Mariko Hyodo, Masumi Horikawa, Mitsuyo Tsunoda, Tetsuto Sato, Masao |
| description | Two pigmented compounds from Uroleucon nigrotuberculatum, rhododactynaphin-jc-1 (H427) and rhododactynaphin-jc-2 (H373), significantly diminished the cell viability of HL60 cells with IC50 of 10 μM and 30 μM, respectively, in an 18 h-dye uptake assay. Both H427 and H373 augmented the levels of intracellular reactive oxygen species (ROS) and induced apoptosis as demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis. ROS augmentation by both H427 and H373 was inhibited by N-acetylcysteine (NAC) and α-tocopherol. The apoptosis induced by H427 was inhibited efficiently with NAC and caspase-8 inhibitor but less efficiently with α-tocopherol and caspase-9 inhibitor. These findings suggested that these pigments have pro-apoptotic activities via oxidative stress. |
| doi_str_mv | 10.1248/bpb.29.2383 |
| format | Article |
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Both H427 and H373 augmented the levels of intracellular reactive oxygen species (ROS) and induced apoptosis as demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis. ROS augmentation by both H427 and H373 was inhibited by N-acetylcysteine (NAC) and α-tocopherol. The apoptosis induced by H427 was inhibited efficiently with NAC and caspase-8 inhibitor but less efficiently with α-tocopherol and caspase-9 inhibitor. These findings suggested that these pigments have pro-apoptotic activities via oxidative stress.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.29.2383</identifier><identifier>PMID: 17142968</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Acetylcysteine - pharmacology ; alpha-Tocopherol - pharmacology ; Animals ; aphid ; Aphids - chemistry ; apoptosis ; Apoptosis - drug effects ; caspase ; Caspases - metabolism ; Enzyme Activation ; HL-60 Cells ; Humans ; In Situ Nick-End Labeling ; Oxidative Stress ; Pigments, Biological - pharmacology ; reactive oxygen species</subject><ispartof>Biological and Pharmaceutical Bulletin, 2006, Vol.29(12), pp.2383-2387</ispartof><rights>2006 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-91ca92ecb526c358e568882e4ffedad1498240974703730d8b58729af03a040c3</citedby><cites>FETCH-LOGICAL-c691t-91ca92ecb526c358e568882e4ffedad1498240974703730d8b58729af03a040c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17142968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Shinya</creatorcontrib><creatorcontrib>Tomita, Mariko</creatorcontrib><creatorcontrib>Hyodo, Masumi</creatorcontrib><creatorcontrib>Horikawa, Mitsuyo</creatorcontrib><creatorcontrib>Tsunoda, Tetsuto</creatorcontrib><creatorcontrib>Sato, Masao</creatorcontrib><creatorcontrib>Facully of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Tokushima Bunri University</creatorcontrib><title>Pigments from Uroleucon nigrotuberculatum Induce Apoptosis in HL60 Human Leukemia Cells, Implicating Intracellular Oxidative Stress and Activation of Caspases</title><title>Biological & Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Two pigmented compounds from Uroleucon nigrotuberculatum, rhododactynaphin-jc-1 (H427) and rhododactynaphin-jc-2 (H373), significantly diminished the cell viability of HL60 cells with IC50 of 10 μM and 30 μM, respectively, in an 18 h-dye uptake assay. Both H427 and H373 augmented the levels of intracellular reactive oxygen species (ROS) and induced apoptosis as demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis. ROS augmentation by both H427 and H373 was inhibited by N-acetylcysteine (NAC) and α-tocopherol. The apoptosis induced by H427 was inhibited efficiently with NAC and caspase-8 inhibitor but less efficiently with α-tocopherol and caspase-9 inhibitor. These findings suggested that these pigments have pro-apoptotic activities via oxidative stress.</description><subject>Acetylcysteine - pharmacology</subject><subject>alpha-Tocopherol - pharmacology</subject><subject>Animals</subject><subject>aphid</subject><subject>Aphids - chemistry</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>caspase</subject><subject>Caspases - metabolism</subject><subject>Enzyme Activation</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Oxidative Stress</subject><subject>Pigments, Biological - pharmacology</subject><subject>reactive oxygen species</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkVuLFDEQhRtR3HH1yXcJCL5oj7n0JXkcB90ZGFhB9zmk09Vjxu6kzUX0z_hbTTOzLvhSgaqvzilyiuIlwWtCK_6-m7s1FWvKOHtUrAir2rKmpH5crLAgvGxIza-KZyGcMMYtpuxpcUVaUlHR8FXx57M5TmBjQIN3E7rzboSknUXWHL2LqQOv06himtDe9kkD2sxuji6YgIxFu0OD0S5NyqIDpO8wGYW2MI7hHdpP82i0isYe82r0Sud-lvLo9pfpc_8noC_RQwhI2R5tdO7kbrZ2A9qqMKsA4XnxZFBjgBeX97q4-_Tx63ZXHm5v9tvNodSNILEURCtBQXc1bTSrOdQN55xCNQzQq55UgtMKi7ZqMWsZ7nlX85YKNWCmcIU1uy7enHVn734kCFFOJiwHKwsuBdlwiptMZvD1f-DJJW_zbZJUlcjehPNMvT1T2rsQPAxy9mZS_rckWC6hyRyapEIuoWX61UUzdRP0D-wlpQzcnIE8zV86OjsaCw_OOrSdcaOTFONGYkwFoRJnq0V-KS3BDResykofzkqnENUR_lkpH40e4f6svH6pi8D9UH9TXoJlfwH4GMG6</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Suzuki, Shinya</creator><creator>Tomita, Mariko</creator><creator>Hyodo, Masumi</creator><creator>Horikawa, Mitsuyo</creator><creator>Tsunoda, Tetsuto</creator><creator>Sato, Masao</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Pigments from Uroleucon nigrotuberculatum Induce Apoptosis in HL60 Human Leukemia Cells, Implicating Intracellular Oxidative Stress and Activation of Caspases</title><author>Suzuki, Shinya ; 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Both H427 and H373 augmented the levels of intracellular reactive oxygen species (ROS) and induced apoptosis as demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis. ROS augmentation by both H427 and H373 was inhibited by N-acetylcysteine (NAC) and α-tocopherol. The apoptosis induced by H427 was inhibited efficiently with NAC and caspase-8 inhibitor but less efficiently with α-tocopherol and caspase-9 inhibitor. These findings suggested that these pigments have pro-apoptotic activities via oxidative stress.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>17142968</pmid><doi>10.1248/bpb.29.2383</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylcysteine - pharmacology alpha-Tocopherol - pharmacology Animals aphid Aphids - chemistry apoptosis Apoptosis - drug effects caspase Caspases - metabolism Enzyme Activation HL-60 Cells Humans In Situ Nick-End Labeling Oxidative Stress Pigments, Biological - pharmacology reactive oxygen species |
| title | Pigments from Uroleucon nigrotuberculatum Induce Apoptosis in HL60 Human Leukemia Cells, Implicating Intracellular Oxidative Stress and Activation of Caspases |
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