Identification and characterization of transcriptional regulation of the Mannheimia haemolytica ferric uptake regulator
The ferric uptake regulator (Fur) is an iron-dependent transcriptional regulator that regulates genes related to iron acquisition, oxidative stress response, and various other functions. Transcription of fur is typically self-regulating and sensitive to iron and oxidative stress. Following the ident...
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description | The ferric uptake regulator (Fur) is an iron-dependent transcriptional regulator that regulates genes related to iron acquisition, oxidative stress response, and various other functions. Transcription of
fur is typically self-regulating and sensitive to iron and oxidative stress. Following the identification of a
fur gene in the genome of the bovine pathogen
Mannheimia haemolytica, an attempt was made to characterize the transcriptional control of
M. haemolytica fur. Northern blotting, RT-PCR, and primer extension were done to determine that
M. haemolytica fur is transcribed using three distinct promoters, two of which are located within the upstream
fldA gene. The third promoter is located upstream of a conserved hypothetical protein and drives transcription of a tricistronic message. Quantitative real time PCR experiments indicated that unlike current models of Fur regulation,
M. haemolytica fur transcription is unchanged by iron depletion at logarithmic phase and repressed by iron depletion at stationary phase. |
doi_str_mv | 10.1016/j.vetmic.2007.04.035 |
format | Article |
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fur is typically self-regulating and sensitive to iron and oxidative stress. Following the identification of a
fur gene in the genome of the bovine pathogen
Mannheimia haemolytica, an attempt was made to characterize the transcriptional control of
M. haemolytica fur. Northern blotting, RT-PCR, and primer extension were done to determine that
M. haemolytica fur is transcribed using three distinct promoters, two of which are located within the upstream
fldA gene. The third promoter is located upstream of a conserved hypothetical protein and drives transcription of a tricistronic message. Quantitative real time PCR experiments indicated that unlike current models of Fur regulation,
M. haemolytica fur transcription is unchanged by iron depletion at logarithmic phase and repressed by iron depletion at stationary phase.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2007.04.035</identifier><identifier>PMID: 17544233</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; beta-Galactosidase - genetics ; Biological and medical sciences ; Blotting, Northern - methods ; Blotting, Northern - veterinary ; DNA primers ; Ferric uptake regulator ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; genes ; Genetics ; Iron ; Iron - deficiency ; Iron - metabolism ; Mannheimia haemolytica ; Mannheimia haemolytica - enzymology ; Mannheimia haemolytica - genetics ; Mannheimia haemolytica - metabolism ; microbial genetics ; microbial physiology ; Microbiology ; molecular cloning ; molecular genetics ; Molecular Sequence Data ; Northern blotting ; operon ; Oxidative Stress ; physiological regulation ; promoter regions ; Promoter Regions, Genetic ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; reverse transcriptase polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; Superoxide Dismutase - genetics ; transcription (genetics) ; Transcription regulation ; Transcription, Genetic ; uptake mechanisms</subject><ispartof>Veterinary microbiology, 2007-10, Vol.124 (3), p.298-309</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-648f0afbf89b3c3925c7f066939d6114c26d7a7cbb387a15f004245dd4daf5513</citedby><cites>FETCH-LOGICAL-c445t-648f0afbf89b3c3925c7f066939d6114c26d7a7cbb387a15f004245dd4daf5513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2007.04.035$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19033411$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17544233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gioia, Jason</creatorcontrib><creatorcontrib>Highlander, Sarah K.</creatorcontrib><title>Identification and characterization of transcriptional regulation of the Mannheimia haemolytica ferric uptake regulator</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The ferric uptake regulator (Fur) is an iron-dependent transcriptional regulator that regulates genes related to iron acquisition, oxidative stress response, and various other functions. Transcription of
fur is typically self-regulating and sensitive to iron and oxidative stress. Following the identification of a
fur gene in the genome of the bovine pathogen
Mannheimia haemolytica, an attempt was made to characterize the transcriptional control of
M. haemolytica fur. Northern blotting, RT-PCR, and primer extension were done to determine that
M. haemolytica fur is transcribed using three distinct promoters, two of which are located within the upstream
fldA gene. The third promoter is located upstream of a conserved hypothetical protein and drives transcription of a tricistronic message. Quantitative real time PCR experiments indicated that unlike current models of Fur regulation,
M. haemolytica fur transcription is unchanged by iron depletion at logarithmic phase and repressed by iron depletion at stationary phase.</description><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>beta-Galactosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern - methods</subject><subject>Blotting, Northern - veterinary</subject><subject>DNA primers</subject><subject>Ferric uptake regulator</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>genes</subject><subject>Genetics</subject><subject>Iron</subject><subject>Iron - deficiency</subject><subject>Iron - metabolism</subject><subject>Mannheimia haemolytica</subject><subject>Mannheimia haemolytica - enzymology</subject><subject>Mannheimia haemolytica - genetics</subject><subject>Mannheimia haemolytica - metabolism</subject><subject>microbial genetics</subject><subject>microbial physiology</subject><subject>Microbiology</subject><subject>molecular cloning</subject><subject>molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Northern blotting</subject><subject>operon</subject><subject>Oxidative Stress</subject><subject>physiological regulation</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>Superoxide Dismutase - genetics</subject><subject>transcription (genetics)</subject><subject>Transcription regulation</subject><subject>Transcription, Genetic</subject><subject>uptake mechanisms</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUlvFDEQhS0EIpPAP0DQF7j1UN56uSBFEYRIQRwgZ6vaS8ZDL4PtDgq_Hrd60NzgZKn8vefye4S8orClQKv3--2DTYPXWwZQb0FsgcsnZEObmpdMCvaUbIDXTUkpl2fkPMY9AIi2gufkjNZSCMb5hvy6MXZM3nmNyU9jgaMp9A4D6mSD_70OJ1ekgGPUwR-WAfZFsPdzf7rd2eILjuPO-sFjsUM7TP1jyqaFsyF4XcyHhD_sX9kUXpBnDvtoXx7PC3L36eP3q8_l7dfrm6vL21ILIVNZicYBus41bcc1b5nUtYOqanlrKkqFZpWpsdZdx5saqXT5i0xIY4RBJyXlF-Td6nsI08_ZxqQGH7XtexztNEdVNQwkFe1_QQYih1ovjmIFdZhiDNapQ_ADhkdFQS3NqL1am1FLMwqEys1k2euj_9wN1pxExyoy8PYIYNTYuxy49vHEtcC5oMv7b1bO4aTwPmTm7hsDygEaxoA3mfiwEjYH--BtUFF7O2prfLA6KTP5f-_6B_lGucE</recordid><startdate>20071006</startdate><enddate>20071006</enddate><creator>Gioia, Jason</creator><creator>Highlander, Sarah K.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20071006</creationdate><title>Identification and characterization of transcriptional regulation of the Mannheimia haemolytica ferric uptake regulator</title><author>Gioia, Jason ; Highlander, Sarah K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-648f0afbf89b3c3925c7f066939d6114c26d7a7cbb387a15f004245dd4daf5513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>beta-Galactosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern - methods</topic><topic>Blotting, Northern - veterinary</topic><topic>DNA primers</topic><topic>Ferric uptake regulator</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>genes</topic><topic>Genetics</topic><topic>Iron</topic><topic>Iron - deficiency</topic><topic>Iron - metabolism</topic><topic>Mannheimia haemolytica</topic><topic>Mannheimia haemolytica - enzymology</topic><topic>Mannheimia haemolytica - genetics</topic><topic>Mannheimia haemolytica - metabolism</topic><topic>microbial genetics</topic><topic>microbial physiology</topic><topic>Microbiology</topic><topic>molecular cloning</topic><topic>molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Northern blotting</topic><topic>operon</topic><topic>Oxidative Stress</topic><topic>physiological regulation</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>Superoxide Dismutase - genetics</topic><topic>transcription (genetics)</topic><topic>Transcription regulation</topic><topic>Transcription, Genetic</topic><topic>uptake mechanisms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gioia, Jason</creatorcontrib><creatorcontrib>Highlander, Sarah K.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gioia, Jason</au><au>Highlander, Sarah K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of transcriptional regulation of the Mannheimia haemolytica ferric uptake regulator</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2007-10-06</date><risdate>2007</risdate><volume>124</volume><issue>3</issue><spage>298</spage><epage>309</epage><pages>298-309</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The ferric uptake regulator (Fur) is an iron-dependent transcriptional regulator that regulates genes related to iron acquisition, oxidative stress response, and various other functions. Transcription of
fur is typically self-regulating and sensitive to iron and oxidative stress. Following the identification of a
fur gene in the genome of the bovine pathogen
Mannheimia haemolytica, an attempt was made to characterize the transcriptional control of
M. haemolytica fur. Northern blotting, RT-PCR, and primer extension were done to determine that
M. haemolytica fur is transcribed using three distinct promoters, two of which are located within the upstream
fldA gene. The third promoter is located upstream of a conserved hypothetical protein and drives transcription of a tricistronic message. Quantitative real time PCR experiments indicated that unlike current models of Fur regulation,
M. haemolytica fur transcription is unchanged by iron depletion at logarithmic phase and repressed by iron depletion at stationary phase.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17544233</pmid><doi>10.1016/j.vetmic.2007.04.035</doi><tpages>12</tpages></addata></record> |
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subjects | Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology beta-Galactosidase - genetics Biological and medical sciences Blotting, Northern - methods Blotting, Northern - veterinary DNA primers Ferric uptake regulator Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial genes Genetics Iron Iron - deficiency Iron - metabolism Mannheimia haemolytica Mannheimia haemolytica - enzymology Mannheimia haemolytica - genetics Mannheimia haemolytica - metabolism microbial genetics microbial physiology Microbiology molecular cloning molecular genetics Molecular Sequence Data Northern blotting operon Oxidative Stress physiological regulation promoter regions Promoter Regions, Genetic Repressor Proteins - genetics Repressor Proteins - metabolism reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - veterinary Superoxide Dismutase - genetics transcription (genetics) Transcription regulation Transcription, Genetic uptake mechanisms |
title | Identification and characterization of transcriptional regulation of the Mannheimia haemolytica ferric uptake regulator |
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