A Clean, More Efficient Method for In-Solution Digestion of Protein Mixtures without Detergent or Urea

Proteolytic digestion of a complicated protein mixture from an organelle or whole-cell lysate is usually carried out in a dilute solution of a denaturing buffer, such as 1−2 M urea. Urea must be subsequently removed by C18 beads before downstream analysis such as HPLC/MS/MS or complete methylation f...

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Veröffentlicht in:	Journal of proteome research 2006-12, Vol.5 (12), p.3446-3452
Hauptverfasser:	Kim, Sung Chan, Chen, Yue, Mirza, Shama, Xu, Yingda, Lee, Jaeick, Liu, Pingsheng, Zhao, Yingming
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Cell Extracts - chemistry Chemical Precipitation Computational Biology HeLa Cells Humans Mass Spectrometry Molecular Sequence Data Proteins - chemistry Proteins - isolation & purification Proteomics - methods Trichloroacetic Acid Trypsin
Online-Zugang:	Volltext
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