Regulation of estrogen receptor α by estradiol in pregnant and estradiol treated rats
Estrogens play an important role in tissue metabolism through specific regulation of several intracellular pathways. We studied ERα regulation in muscle and adipose tissue from pregnant and estradiol treated rats. In both groups, we identified three different ERα inmunoreactive proteins (80, 67 and...
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| Veröffentlicht in: | Steroids 2006-12, Vol.71 (13), p.1052-1061 |
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| creator | Alonso, Ana Fernández, Rebeca Ordóñez, Patricia Moreno, María Patterson, Ángeles M. González, Celestino |
| description | Estrogens play an important role in tissue metabolism through specific regulation of several intracellular pathways. We studied ERα regulation in muscle and adipose tissue from pregnant and estradiol treated rats. In both groups, we identified three different ERα inmunoreactive proteins (80, 67 and 46
kDa) using total protein extracts. Because it has been showed that estrogens are able to promote rapid effects in several cellular models, we looked for three ERα-related proteins at plasma membrane. In skeletal muscle of both groups, we positively identified the three ERα-related isoforms in plasma membrane, but in adipose tissue from pregnant we were not able to identify ERα67, and in estradiol treated animals ERα80 was absent. Taking together, our results showed a tissue-specific regulation of whole-cell ERα-related proteins and ERα located at plasma membrane, which should be involved in non-genomic actions of 17β-estradiol. The role of the three ERα inmunoreactive proteins is unknown, however, seems probably related to rapid activation of signalling pathways. |
| doi_str_mv | 10.1016/j.steroids.2006.09.004 |
| format | Article |
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kDa) using total protein extracts. Because it has been showed that estrogens are able to promote rapid effects in several cellular models, we looked for three ERα-related proteins at plasma membrane. In skeletal muscle of both groups, we positively identified the three ERα-related isoforms in plasma membrane, but in adipose tissue from pregnant we were not able to identify ERα67, and in estradiol treated animals ERα80 was absent. Taking together, our results showed a tissue-specific regulation of whole-cell ERα-related proteins and ERα located at plasma membrane, which should be involved in non-genomic actions of 17β-estradiol. The role of the three ERα inmunoreactive proteins is unknown, however, seems probably related to rapid activation of signalling pathways.</description><identifier>ISSN: 0039-128X</identifier><identifier>EISSN: 1878-5867</identifier><identifier>DOI: 10.1016/j.steroids.2006.09.004</identifier><identifier>PMID: 17030051</identifier><identifier>CODEN: STEDAM</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Western ; Cell Membrane - metabolism ; Estradiol - pharmacology ; Estradiol treatment ; Estrogen receptor ; Estrogen Receptor alpha - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Pregnancy ; Rat ; Rats ; Vertebrates: endocrinology</subject><ispartof>Steroids, 2006-12, Vol.71 (13), p.1052-1061</ispartof><rights>2006 Elsevier Inc.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-3f241d24e74843c1c40494f5bcbe1196bd71cc19cff203ccf8cfb7601687f5e13</citedby><cites>FETCH-LOGICAL-c396t-3f241d24e74843c1c40494f5bcbe1196bd71cc19cff203ccf8cfb7601687f5e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.steroids.2006.09.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18336450$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17030051$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alonso, Ana</creatorcontrib><creatorcontrib>Fernández, Rebeca</creatorcontrib><creatorcontrib>Ordóñez, Patricia</creatorcontrib><creatorcontrib>Moreno, María</creatorcontrib><creatorcontrib>Patterson, Ángeles M.</creatorcontrib><creatorcontrib>González, Celestino</creatorcontrib><title>Regulation of estrogen receptor α by estradiol in pregnant and estradiol treated rats</title><title>Steroids</title><addtitle>Steroids</addtitle><description>Estrogens play an important role in tissue metabolism through specific regulation of several intracellular pathways. We studied ERα regulation in muscle and adipose tissue from pregnant and estradiol treated rats. In both groups, we identified three different ERα inmunoreactive proteins (80, 67 and 46
kDa) using total protein extracts. Because it has been showed that estrogens are able to promote rapid effects in several cellular models, we looked for three ERα-related proteins at plasma membrane. In skeletal muscle of both groups, we positively identified the three ERα-related isoforms in plasma membrane, but in adipose tissue from pregnant we were not able to identify ERα67, and in estradiol treated animals ERα80 was absent. Taking together, our results showed a tissue-specific regulation of whole-cell ERα-related proteins and ERα located at plasma membrane, which should be involved in non-genomic actions of 17β-estradiol. The role of the three ERα inmunoreactive proteins is unknown, however, seems probably related to rapid activation of signalling pathways.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Membrane - metabolism</subject><subject>Estradiol - pharmacology</subject><subject>Estradiol treatment</subject><subject>Estrogen receptor</subject><subject>Estrogen Receptor alpha - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Pregnancy</topic><topic>Rat</topic><topic>Rats</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alonso, Ana</creatorcontrib><creatorcontrib>Fernández, Rebeca</creatorcontrib><creatorcontrib>Ordóñez, Patricia</creatorcontrib><creatorcontrib>Moreno, María</creatorcontrib><creatorcontrib>Patterson, Ángeles M.</creatorcontrib><creatorcontrib>González, Celestino</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alonso, Ana</au><au>Fernández, Rebeca</au><au>Ordóñez, Patricia</au><au>Moreno, María</au><au>Patterson, Ángeles M.</au><au>González, Celestino</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of estrogen receptor α by estradiol in pregnant and estradiol treated rats</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>71</volume><issue>13</issue><spage>1052</spage><epage>1061</epage><pages>1052-1061</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><coden>STEDAM</coden><abstract>Estrogens play an important role in tissue metabolism through specific regulation of several intracellular pathways. 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kDa) using total protein extracts. Because it has been showed that estrogens are able to promote rapid effects in several cellular models, we looked for three ERα-related proteins at plasma membrane. In skeletal muscle of both groups, we positively identified the three ERα-related isoforms in plasma membrane, but in adipose tissue from pregnant we were not able to identify ERα67, and in estradiol treated animals ERα80 was absent. Taking together, our results showed a tissue-specific regulation of whole-cell ERα-related proteins and ERα located at plasma membrane, which should be involved in non-genomic actions of 17β-estradiol. The role of the three ERα inmunoreactive proteins is unknown, however, seems probably related to rapid activation of signalling pathways.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>17030051</pmid><doi>10.1016/j.steroids.2006.09.004</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Blotting, Western Cell Membrane - metabolism Estradiol - pharmacology Estradiol treatment Estrogen receptor Estrogen Receptor alpha - physiology Female Fundamental and applied biological sciences. Psychology Pregnancy Rat Rats Vertebrates: endocrinology |
| title | Regulation of estrogen receptor α by estradiol in pregnant and estradiol treated rats |
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