NuMA distribution and microtubule configuration in rabbit oocytes and cloned embryos
The assembly of microtubules and the distribution of NuMA were analyzed in rabbit oocytes and early cloned embryos. α-Tubulin was localized around the periphery of the germinal vesicle (GV). After germinal vesicle breakdown (GVBD), multi-arrayed microtubules were found tightly associated with the co...
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| Veröffentlicht in: | Reproduction (Cambridge, England) England), 2006-12, Vol.132 (6), p.869-876 |
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| creator | Yan, Li-Ying Huang, Jun-Cheng Zhu, Zi-Yu Lei, Zi-Li Shi, Li-Hong Nan, Chang-Long Zhao, Zhen-Jun OuYang, Ying-Chun Song, Xiang-Fen Sun, Qing-Yuan Chen, Da-Yuan |
| description | The assembly of microtubules and the distribution of NuMA were analyzed in rabbit oocytes and early cloned embryos. α-Tubulin was localized around the periphery of the germinal vesicle (GV). After germinal vesicle breakdown (GVBD), multi-arrayed microtubules were found tightly associated with the condensed chromosomes and assembled into spindles. After the enucleated oocyte was fused with a fibroblast, microtubules were observed around the introduced nucleus in most reconstructed embryos and formed a transient spindle 2–4 h post-fusion (hpf). A mass of microtubules surrounded the swollen pseudo-pronucleus 5 hpf and a normal spindle was formed 13 hpf in cloned embryos. NuMAwas detected in the nucleus in germinal vesicle-stage oocytes, and it was concentrated at the spindle poles in both meiotic and mitotic metaphase. In both donor cell nucleus and enucleated oocyte cytoplasm, NuMA was not detected, while NuMA reappeared in pseudo-pronucleus as reconstructed embryo development proceeded. However, no evident NuMA staining was observed in the poles of transient spindle and first mitotic spindle in nuclear transfer eggs. These results indicate that NuMA localization and its spindle pole tethering function are different during rabbit oocyte meiosis and cloned embryo mitosis. |
| doi_str_mv | 10.1530/rep.1.01224 |
| format | Article |
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After germinal vesicle breakdown (GVBD), multi-arrayed microtubules were found tightly associated with the condensed chromosomes and assembled into spindles. After the enucleated oocyte was fused with a fibroblast, microtubules were observed around the introduced nucleus in most reconstructed embryos and formed a transient spindle 2–4 h post-fusion (hpf). A mass of microtubules surrounded the swollen pseudo-pronucleus 5 hpf and a normal spindle was formed 13 hpf in cloned embryos. NuMAwas detected in the nucleus in germinal vesicle-stage oocytes, and it was concentrated at the spindle poles in both meiotic and mitotic metaphase. In both donor cell nucleus and enucleated oocyte cytoplasm, NuMA was not detected, while NuMA reappeared in pseudo-pronucleus as reconstructed embryo development proceeded. However, no evident NuMA staining was observed in the poles of transient spindle and first mitotic spindle in nuclear transfer eggs. These results indicate that NuMA localization and its spindle pole tethering function are different during rabbit oocyte meiosis and cloned embryo mitosis.</description><identifier>ISSN: 1470-1626</identifier><identifier>EISSN: 1741-7899</identifier><identifier>DOI: 10.1530/rep.1.01224</identifier><identifier>PMID: 17127747</identifier><language>eng</language><publisher>Colchester: Society for Reproduction and Fertility</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; cloning (animals) ; Cloning, Organism ; Early stages. Segmentation. Gastrulation. Neurulation ; Embryo, Mammalian - chemistry ; Embryo, Mammalian - ultrastructure ; embryogenesis ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; germinal vesicle ; Male ; Meiosis ; Metaphase ; Microscopy, Confocal ; microtubules ; Microtubules - ultrastructure ; Mitosis ; Nuclear Matrix-Associated Proteins - analysis ; Nuclear Transfer Techniques ; nuclear transplantation ; Oocytes - chemistry ; Oocytes - ultrastructure ; Rabbits ; Spindle Apparatus - ultrastructure ; tubulin ; Tubulin - ultrastructure ; Vertebrates: reproduction</subject><ispartof>Reproduction (Cambridge, England), 2006-12, Vol.132 (6), p.869-876</ispartof><rights>2006 Society for Reproduction and Fertility</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b526t-753db95e948906c310f576885820df8bc00ae7442e00fcf9ab36e6cee6edd4523</citedby><cites>FETCH-LOGICAL-b526t-753db95e948906c310f576885820df8bc00ae7442e00fcf9ab36e6cee6edd4523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18345702$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17127747$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Li-Ying</creatorcontrib><creatorcontrib>Huang, Jun-Cheng</creatorcontrib><creatorcontrib>Zhu, Zi-Yu</creatorcontrib><creatorcontrib>Lei, Zi-Li</creatorcontrib><creatorcontrib>Shi, Li-Hong</creatorcontrib><creatorcontrib>Nan, Chang-Long</creatorcontrib><creatorcontrib>Zhao, Zhen-Jun</creatorcontrib><creatorcontrib>OuYang, Ying-Chun</creatorcontrib><creatorcontrib>Song, Xiang-Fen</creatorcontrib><creatorcontrib>Sun, Qing-Yuan</creatorcontrib><creatorcontrib>Chen, Da-Yuan</creatorcontrib><title>NuMA distribution and microtubule configuration in rabbit oocytes and cloned embryos</title><title>Reproduction (Cambridge, England)</title><addtitle>Reproduction</addtitle><description>The assembly of microtubules and the distribution of NuMA were analyzed in rabbit oocytes and early cloned embryos. α-Tubulin was localized around the periphery of the germinal vesicle (GV). After germinal vesicle breakdown (GVBD), multi-arrayed microtubules were found tightly associated with the condensed chromosomes and assembled into spindles. After the enucleated oocyte was fused with a fibroblast, microtubules were observed around the introduced nucleus in most reconstructed embryos and formed a transient spindle 2–4 h post-fusion (hpf). A mass of microtubules surrounded the swollen pseudo-pronucleus 5 hpf and a normal spindle was formed 13 hpf in cloned embryos. NuMAwas detected in the nucleus in germinal vesicle-stage oocytes, and it was concentrated at the spindle poles in both meiotic and mitotic metaphase. In both donor cell nucleus and enucleated oocyte cytoplasm, NuMA was not detected, while NuMA reappeared in pseudo-pronucleus as reconstructed embryo development proceeded. However, no evident NuMA staining was observed in the poles of transient spindle and first mitotic spindle in nuclear transfer eggs. These results indicate that NuMA localization and its spindle pole tethering function are different during rabbit oocyte meiosis and cloned embryo mitosis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>cloning (animals)</subject><subject>Cloning, Organism</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>Embryo, Mammalian - chemistry</subject><subject>Embryo, Mammalian - ultrastructure</subject><subject>embryogenesis</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>germinal vesicle</subject><subject>Male</subject><subject>Meiosis</subject><subject>Metaphase</subject><subject>Microscopy, Confocal</subject><subject>microtubules</subject><subject>Microtubules - ultrastructure</subject><subject>Mitosis</subject><subject>Nuclear Matrix-Associated Proteins - analysis</subject><subject>Nuclear Transfer Techniques</subject><subject>nuclear transplantation</subject><subject>Oocytes - chemistry</subject><subject>Oocytes - ultrastructure</subject><subject>Rabbits</subject><subject>Spindle Apparatus - ultrastructure</subject><subject>tubulin</subject><subject>Tubulin - ultrastructure</subject><subject>Vertebrates: reproduction</subject><issn>1470-1626</issn><issn>1741-7899</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0Etv1DAUBeAIgegDVuwhG9igDNeP2M6yqqAgFVjQri3buZ4aJfFgJ6rm3-NORqqEBKxsyZ-PfU9VvSKwIS2DDwl3G7IBQil_Up0SyUkjVdc9LXsuoSGCipPqLOefAKRVUjyvTogkVEouT6ubb8vXi7oPeU7BLnOIU22mvh6DS3Fe7DJg7eLkw3ZJ5nAapjoZa8Ncx-j2M-aDd0OcsK9xtGkf84vqmTdDxpfH9by6_fTx5vJzc_396svlxXVjWyrmRrast12LHVcdCMcI-FYKpVpFoffKOgCDknOKAN75zlgmUDhEgX3PW8rOq3dr7i7FXwvmWY8hOxwGM2FcshaKqNIE_BdSkKxjjBf4foVl_JwTer1LYTRprwnoh7Z1aVsTfWi76NfH2MWO2D_aY70FvD0Ck50ZfDKTC_nRKcZbCQ-D0NXdhe3dfUiobYjZBZzm4IMzf3n9zXrJm6jNNpXg2x8UCANCyg-UKoKs4o-0f830G1wZss4</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Yan, Li-Ying</creator><creator>Huang, Jun-Cheng</creator><creator>Zhu, Zi-Yu</creator><creator>Lei, Zi-Li</creator><creator>Shi, Li-Hong</creator><creator>Nan, Chang-Long</creator><creator>Zhao, Zhen-Jun</creator><creator>OuYang, Ying-Chun</creator><creator>Song, Xiang-Fen</creator><creator>Sun, Qing-Yuan</creator><creator>Chen, Da-Yuan</creator><general>Society for Reproduction and Fertility</general><general>BioScientifica Ltd</general><general>Portland</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>NuMA distribution and microtubule configuration in rabbit oocytes and cloned embryos</title><author>Yan, Li-Ying ; Huang, Jun-Cheng ; Zhu, Zi-Yu ; Lei, Zi-Li ; Shi, Li-Hong ; Nan, Chang-Long ; Zhao, Zhen-Jun ; OuYang, Ying-Chun ; Song, Xiang-Fen ; Sun, Qing-Yuan ; Chen, Da-Yuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b526t-753db95e948906c310f576885820df8bc00ae7442e00fcf9ab36e6cee6edd4523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>cloning (animals)</topic><topic>Cloning, Organism</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>Embryo, Mammalian - chemistry</topic><topic>Embryo, Mammalian - ultrastructure</topic><topic>embryogenesis</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>germinal vesicle</topic><topic>Male</topic><topic>Meiosis</topic><topic>Metaphase</topic><topic>Microscopy, Confocal</topic><topic>microtubules</topic><topic>Microtubules - ultrastructure</topic><topic>Mitosis</topic><topic>Nuclear Matrix-Associated Proteins - analysis</topic><topic>Nuclear Transfer Techniques</topic><topic>nuclear transplantation</topic><topic>Oocytes - chemistry</topic><topic>Oocytes - ultrastructure</topic><topic>Rabbits</topic><topic>Spindle Apparatus - ultrastructure</topic><topic>tubulin</topic><topic>Tubulin - ultrastructure</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Li-Ying</creatorcontrib><creatorcontrib>Huang, Jun-Cheng</creatorcontrib><creatorcontrib>Zhu, Zi-Yu</creatorcontrib><creatorcontrib>Lei, Zi-Li</creatorcontrib><creatorcontrib>Shi, Li-Hong</creatorcontrib><creatorcontrib>Nan, Chang-Long</creatorcontrib><creatorcontrib>Zhao, Zhen-Jun</creatorcontrib><creatorcontrib>OuYang, Ying-Chun</creatorcontrib><creatorcontrib>Song, Xiang-Fen</creatorcontrib><creatorcontrib>Sun, Qing-Yuan</creatorcontrib><creatorcontrib>Chen, Da-Yuan</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Li-Ying</au><au>Huang, Jun-Cheng</au><au>Zhu, Zi-Yu</au><au>Lei, Zi-Li</au><au>Shi, Li-Hong</au><au>Nan, Chang-Long</au><au>Zhao, Zhen-Jun</au><au>OuYang, Ying-Chun</au><au>Song, Xiang-Fen</au><au>Sun, Qing-Yuan</au><au>Chen, Da-Yuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NuMA distribution and microtubule configuration in rabbit oocytes and cloned embryos</atitle><jtitle>Reproduction (Cambridge, England)</jtitle><addtitle>Reproduction</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>132</volume><issue>6</issue><spage>869</spage><epage>876</epage><pages>869-876</pages><issn>1470-1626</issn><eissn>1741-7899</eissn><abstract>The assembly of microtubules and the distribution of NuMA were analyzed in rabbit oocytes and early cloned embryos. α-Tubulin was localized around the periphery of the germinal vesicle (GV). After germinal vesicle breakdown (GVBD), multi-arrayed microtubules were found tightly associated with the condensed chromosomes and assembled into spindles. After the enucleated oocyte was fused with a fibroblast, microtubules were observed around the introduced nucleus in most reconstructed embryos and formed a transient spindle 2–4 h post-fusion (hpf). A mass of microtubules surrounded the swollen pseudo-pronucleus 5 hpf and a normal spindle was formed 13 hpf in cloned embryos. NuMAwas detected in the nucleus in germinal vesicle-stage oocytes, and it was concentrated at the spindle poles in both meiotic and mitotic metaphase. In both donor cell nucleus and enucleated oocyte cytoplasm, NuMA was not detected, while NuMA reappeared in pseudo-pronucleus as reconstructed embryo development proceeded. However, no evident NuMA staining was observed in the poles of transient spindle and first mitotic spindle in nuclear transfer eggs. These results indicate that NuMA localization and its spindle pole tethering function are different during rabbit oocyte meiosis and cloned embryo mitosis.</abstract><cop>Colchester</cop><pub>Society for Reproduction and Fertility</pub><pmid>17127747</pmid><doi>10.1530/rep.1.01224</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Cells, Cultured cloning (animals) Cloning, Organism Early stages. Segmentation. Gastrulation. Neurulation Embryo, Mammalian - chemistry Embryo, Mammalian - ultrastructure embryogenesis Embryology: invertebrates and vertebrates. Teratology Female Fertilization in Vitro Fundamental and applied biological sciences. Psychology germinal vesicle Male Meiosis Metaphase Microscopy, Confocal microtubules Microtubules - ultrastructure Mitosis Nuclear Matrix-Associated Proteins - analysis Nuclear Transfer Techniques nuclear transplantation Oocytes - chemistry Oocytes - ultrastructure Rabbits Spindle Apparatus - ultrastructure tubulin Tubulin - ultrastructure Vertebrates: reproduction |
| title | NuMA distribution and microtubule configuration in rabbit oocytes and cloned embryos |
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