Characterization of swine leukocyte antigen polymorphism by sequence-based and PCR-SSP methods in Meishan pigs
Resource herds of swine leukocyte antigen (SLA)-characterized pigs are an important tool for the study of immune responses, disease resistance, and production traits. They are also valuable large animal models for biomedical research, such as transplantation. The Meishan breed of pig is an economica...
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| Veröffentlicht in: | Immunogenetics (New York) 2006-11, Vol.58 (11), p.873-882 |
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| creator | Ho, Chak-Sum Rochelle, Erin S Martens, Gregory W Schook, Lawrence B Smith, Douglas M |
| description | Resource herds of swine leukocyte antigen (SLA)-characterized pigs are an important tool for the study of immune responses, disease resistance, and production traits. They are also valuable large animal models for biomedical research, such as transplantation. The Meishan breed of pig is an economically significant breed that is available at several research institutions in the United States. We have characterized the SLA polymorphism of the breeding stock in the herd maintained at the University of Illinois and developed a simple assay to SLA type individuals within that herd. We have used a reverse transcription-polymerase chain reaction (RT-PCR)-based SLA typing method to clone and DNA sequence 19 SLA alleles at three SLA class Ia (SLA-1, SLA-2, and SLA-3) and two SLA class II (SLA-DRB1 and SLA-DQB1) loci. Based on this sequence information, a rapid SLA typing assay was developed to discriminate each allele using PCR with sequence-specific primers (PCR-SSP). Using this method, we were able to characterize the entire Meishan breeding stock and identify four SLA haplotypes present in the herd. The combination of SLA typing by cloning and DNA sequencing with PCR-SSP is therefore a valuable tool for the characterization of SLA alleles and haplotypes in resource herds of pigs. |
| doi_str_mv | 10.1007/s00251-006-0145-y |
| format | Article |
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They are also valuable large animal models for biomedical research, such as transplantation. The Meishan breed of pig is an economically significant breed that is available at several research institutions in the United States. We have characterized the SLA polymorphism of the breeding stock in the herd maintained at the University of Illinois and developed a simple assay to SLA type individuals within that herd. We have used a reverse transcription-polymerase chain reaction (RT-PCR)-based SLA typing method to clone and DNA sequence 19 SLA alleles at three SLA class Ia (SLA-1, SLA-2, and SLA-3) and two SLA class II (SLA-DRB1 and SLA-DQB1) loci. Based on this sequence information, a rapid SLA typing assay was developed to discriminate each allele using PCR with sequence-specific primers (PCR-SSP). Using this method, we were able to characterize the entire Meishan breeding stock and identify four SLA haplotypes present in the herd. The combination of SLA typing by cloning and DNA sequencing with PCR-SSP is therefore a valuable tool for the characterization of SLA alleles and haplotypes in resource herds of pigs.</description><identifier>ISSN: 0093-7711</identifier><identifier>EISSN: 1432-1211</identifier><identifier>DOI: 10.1007/s00251-006-0145-y</identifier><identifier>PMID: 17039361</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Animal models ; Animals ; Base Sequence ; Cloning ; Deoxyribonucleic acid ; Disease resistance ; DNA ; DNA - analysis ; DNA - genetics ; Gene Frequency ; Haplotypes ; Histocompatibility Antigens Class I - genetics ; Histocompatibility Antigens Class II ; Hogs ; Molecular Sequence Data ; Pedigree ; Polymerase Chain Reaction - methods ; Polymorphism, Genetic ; Research institutions ; Swine ; Swine - classification ; Swine - genetics</subject><ispartof>Immunogenetics (New York), 2006-11, Vol.58 (11), p.873-882</ispartof><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-fa65f7e96d3ac8027c1e066f5828c5c1fd61b4c2bafdca6ded490f1c190d46933</citedby><cites>FETCH-LOGICAL-c423t-fa65f7e96d3ac8027c1e066f5828c5c1fd61b4c2bafdca6ded490f1c190d46933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17039361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ho, Chak-Sum</creatorcontrib><creatorcontrib>Rochelle, Erin S</creatorcontrib><creatorcontrib>Martens, Gregory W</creatorcontrib><creatorcontrib>Schook, Lawrence B</creatorcontrib><creatorcontrib>Smith, Douglas M</creatorcontrib><title>Characterization of swine leukocyte antigen polymorphism by sequence-based and PCR-SSP methods in Meishan pigs</title><title>Immunogenetics (New York)</title><addtitle>Immunogenetics</addtitle><description>Resource herds of swine leukocyte antigen (SLA)-characterized pigs are an important tool for the study of immune responses, disease resistance, and production traits. They are also valuable large animal models for biomedical research, such as transplantation. The Meishan breed of pig is an economically significant breed that is available at several research institutions in the United States. We have characterized the SLA polymorphism of the breeding stock in the herd maintained at the University of Illinois and developed a simple assay to SLA type individuals within that herd. We have used a reverse transcription-polymerase chain reaction (RT-PCR)-based SLA typing method to clone and DNA sequence 19 SLA alleles at three SLA class Ia (SLA-1, SLA-2, and SLA-3) and two SLA class II (SLA-DRB1 and SLA-DQB1) loci. Based on this sequence information, a rapid SLA typing assay was developed to discriminate each allele using PCR with sequence-specific primers (PCR-SSP). Using this method, we were able to characterize the entire Meishan breeding stock and identify four SLA haplotypes present in the herd. The combination of SLA typing by cloning and DNA sequencing with PCR-SSP is therefore a valuable tool for the characterization of SLA alleles and haplotypes in resource herds of pigs.</description><subject>Animal models</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning</subject><subject>Deoxyribonucleic acid</subject><subject>Disease resistance</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA - genetics</subject><subject>Gene Frequency</subject><subject>Haplotypes</subject><subject>Histocompatibility Antigens Class I - genetics</subject><subject>Histocompatibility Antigens Class II</subject><subject>Hogs</subject><subject>Molecular Sequence Data</subject><subject>Pedigree</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Genetic</subject><subject>Research institutions</subject><subject>Swine</subject><subject>Swine - classification</subject><subject>Swine - genetics</subject><issn>0093-7711</issn><issn>1432-1211</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1v1DAURS0EotPCD2CDLBbsDH624yRLNOKjUiuqtqwtx37puCT2YCeqwq8noxkJiQ2rtzn36j4dQt4A_wCc1x8L56ICxrlmHFTFlmdkA0oKBgLgOdlw3kpW1wBn5LyUR86haoV-Sc6g5rKVGjYkbnc2WzdhDr_tFFKkqaflKUSkA84_k1smpDZO4QEj3adhGVPe70IZabfQgr9mjA5ZZwv6FfP0ZnvL7u5u6IjTLvlCQ6TXGMrOrunwUF6RF70dCr4-3Qvy48vn--03dvX96-X20xVzSsiJ9VZXfY2t9tK6hovaAXKt-6oRjasc9F5Dp5zobO-d1R69ankPDlrulW6lvCDvj737nNaNZTJjKA6HwUZMczG6gQZaVf8XhLZqQNYH8N0_4GOac1yfMFpIoRToaoXgCLmcSsnYm30Oo82LAW4OysxRmVmVmYMys6yZt6fiuRvR_02cHMk_qquSyg</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Ho, Chak-Sum</creator><creator>Rochelle, Erin S</creator><creator>Martens, Gregory W</creator><creator>Schook, Lawrence B</creator><creator>Smith, Douglas M</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061101</creationdate><title>Characterization of swine leukocyte antigen polymorphism by sequence-based and PCR-SSP methods in Meishan pigs</title><author>Ho, Chak-Sum ; Rochelle, Erin S ; Martens, Gregory W ; Schook, Lawrence B ; Smith, Douglas M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-fa65f7e96d3ac8027c1e066f5828c5c1fd61b4c2bafdca6ded490f1c190d46933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning</topic><topic>Deoxyribonucleic acid</topic><topic>Disease resistance</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA - genetics</topic><topic>Gene Frequency</topic><topic>Haplotypes</topic><topic>Histocompatibility Antigens Class I - genetics</topic><topic>Histocompatibility Antigens Class II</topic><topic>Hogs</topic><topic>Molecular Sequence Data</topic><topic>Pedigree</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Genetic</topic><topic>Research institutions</topic><topic>Swine</topic><topic>Swine - classification</topic><topic>Swine - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ho, Chak-Sum</creatorcontrib><creatorcontrib>Rochelle, Erin S</creatorcontrib><creatorcontrib>Martens, Gregory W</creatorcontrib><creatorcontrib>Schook, Lawrence B</creatorcontrib><creatorcontrib>Smith, Douglas M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunogenetics (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ho, Chak-Sum</au><au>Rochelle, Erin S</au><au>Martens, Gregory W</au><au>Schook, Lawrence B</au><au>Smith, Douglas M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of swine leukocyte antigen polymorphism by sequence-based and PCR-SSP methods in Meishan pigs</atitle><jtitle>Immunogenetics (New York)</jtitle><addtitle>Immunogenetics</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>58</volume><issue>11</issue><spage>873</spage><epage>882</epage><pages>873-882</pages><issn>0093-7711</issn><eissn>1432-1211</eissn><abstract>Resource herds of swine leukocyte antigen (SLA)-characterized pigs are an important tool for the study of immune responses, disease resistance, and production traits. They are also valuable large animal models for biomedical research, such as transplantation. The Meishan breed of pig is an economically significant breed that is available at several research institutions in the United States. We have characterized the SLA polymorphism of the breeding stock in the herd maintained at the University of Illinois and developed a simple assay to SLA type individuals within that herd. We have used a reverse transcription-polymerase chain reaction (RT-PCR)-based SLA typing method to clone and DNA sequence 19 SLA alleles at three SLA class Ia (SLA-1, SLA-2, and SLA-3) and two SLA class II (SLA-DRB1 and SLA-DQB1) loci. Based on this sequence information, a rapid SLA typing assay was developed to discriminate each allele using PCR with sequence-specific primers (PCR-SSP). Using this method, we were able to characterize the entire Meishan breeding stock and identify four SLA haplotypes present in the herd. The combination of SLA typing by cloning and DNA sequencing with PCR-SSP is therefore a valuable tool for the characterization of SLA alleles and haplotypes in resource herds of pigs.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>17039361</pmid><doi>10.1007/s00251-006-0145-y</doi><tpages>10</tpages></addata></record> |
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| subjects | Animal models Animals Base Sequence Cloning Deoxyribonucleic acid Disease resistance DNA DNA - analysis DNA - genetics Gene Frequency Haplotypes Histocompatibility Antigens Class I - genetics Histocompatibility Antigens Class II Hogs Molecular Sequence Data Pedigree Polymerase Chain Reaction - methods Polymorphism, Genetic Research institutions Swine Swine - classification Swine - genetics |
| title | Characterization of swine leukocyte antigen polymorphism by sequence-based and PCR-SSP methods in Meishan pigs |
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